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Suryanti Suryanti
Fakultas Pertanian Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

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Identifikasi Ras Fisiologis Fusarium oxysporum f.sp. cubense Berdasarkan Sifat Kompatibel Secara Vegetatif dan Pembentukan Bahan Volatil Arif Wibowo; Suryanti Suryanti; Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 8, No 1 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (10171.163 KB) | DOI: 10.22146/jpti.10099

Abstract

Race characterization of F. oxysporum Schlecht. f.sp. cubense (E.F. Smith) Snyd. & Hans. by determining disease reaction is difficult because the result may be biased due to the variability of growing condition. This study is aimed to identify physiological races of F. oxysporum f.sp. cubense in banana plantation in the province of Daerah Istimewa Yogyakarta by examining the relation of the fungal pathogen isolates. The identification of physiological races of F. oxysporum f.sp. cubense was based on vegetative compatibility groups (VCGs). The research was conducted in The Laboratory of Mycology Faculty of Agriculture GMU on March to November 2000. Observation of heterokarion formed by the mutant of F. oxysporum f.sp. cubense on selective medium was carried out in order to identify the compatibility of two different fungal isolates. Nitrate non utilizing (nit) mutants obtained without mutagen were used as the label. Nit mutant obtained from the same wild type isolates could form heterokarion on minimal agar medium containing NaNO3 as nitrogen source. Eleven isolates of F. oxysporum f.sp. cubense have been isolated from several cultivars of banana. Vegetative compatibility tests showed that of 11 eleven isolates, there were six different group VCGs where four of them formed volatile compound in rice medium whereas two of them did not.
Identifikasi dan Virulensi Fusarium oxysporum f.sp. cubense RAS 4 Dwi Kiswanti; Suryanti Suryanti; Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 16, No 1 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.166 KB) | DOI: 10.22146/jpti.11748

Abstract

The aim of this study was to identify and to detect the virulence of Fusarium oxysporum f.sp. cubense (Foc) race 4. The isolates consisted of BNT-2, KD-1, U-8, BK, A-2, and A-13. Monospore isolation was done to obtain stable isolates. The detection and characteristics of isolates were observed on Komada medium. The diameter of colony and color was observed from underside of culture in petridish, while the shape of colony was observed from the upper side. Virulence test was conducted on Cavendish cultivar seedlings. Banana seedlings were inoculated with Foc cultured on rice medium (20 g/kg soil). The result indicated that A-13, U-8, BNT-2, and BK, were very virulent isolates; while A-2 and KD-1 were virulent. All isolates were detected as Foc race 4, with mild yellowish color and laccinated colonies on Komada medium.
Pengimbasan Ketahanan Pisang terhadap Penyakit Layu Fusarium dengan Asam Salisilat In Vitro Suryanti Suryanti; Yufita Dwi Chinta; Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11770

Abstract

Salicylic acid (SA) is an important signal in plant defense. It is used as induced resistance agent against Fusarium wilt. An artificial induction was conducted by shaking the shoot groups of banana tissue culture in liquid medium of Murashige-Skoog (MS). MS medium was added with 0 ppm (as control), ⅛ LC50, ¼ LC50, ½ LC50, and LC50 concentration of SA. Alive shoot groups were subcultured for about three months and were acclimated. Resistance test had been conducted in glass house by inoculated six months old banana seedlings with Fusarium oxysporum f.sp. cubense (Foc). Leaves symptom were observed based on Leaf Symptom Index (LSI) and corm discoloration based on Rhizome Discoloration Index (RDI). The result of this research showed that induced banana seedlings had higher plant resistance to Fusarium wilt than control.
Kisaran Inang Hama Krepes Suryanti Suryanti; Tri Harjaka
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11863

Abstract

Krepes is an acarina which caused damaged to Auricularia sp. cultivation. The fungi attacked by acarina will be dead and decayed. The host range test were done by inoculating krepes on several edible mushroom and on several stages of cultivated Auricularia sp. The research show that krepes attacks only Auricularia sp., and attack all stages of cultivated Auricularia sp.,
Pengendalian Penyakit Layu Pisang dengan Fusarium Nonpatogenik dan Fluorescent pseudomonads Christanti Sumardiyono; Arif Wibowo; Suryanti Suryanti
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11865

Abstract

The study was aimed to know the ability of fluorescent pseudomonads and nonpathogenic Fusarium for controlling fusarium wilt of banan. The research was conducted both in the laboratory and glass ouse in the Faculty of Agriculture Gadjah Mada University. Laboratorial trial incuded the examining of antagonistic capacity in vitro and the population of these two antagonistic microorganism in compost medium. The examination of the effect of these two microorganisms in compost medium against fusarium wilt of banana Ambon Kuning cultivar was conducted in a glass house. The result showed that there was no antagonistic mechanism occured between fluorescent pseudomonads and nonpathogenic Fusarium. The treatment of compost with fluorescent pseudomonads or/and nonpathogenic Fusarium showed that there were differences on the optimum population of these two antagonistic microorganisms. Fluorescent pseudomonads attained its optimum population one week after inoculation while nonpathogenic Fusarium attained its optimum population two weeks after inoculation. The glass house trial showed taht compost enriched with two microorganisms with one week inocubation time reduced percentage of wilted leaves, although it was nonsignificant with control treatment. Field experiment should be conducted with higher population density of antagonist microorganisms.
Pengendalian Penyakit Layu Fusarium pada Pisang dengan Inokulasi Jamur Mikoriza Vesikular Arbuskular pada Bibit Suryanti Suryanti; Arif Wibowo; Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9375.633 KB) | DOI: 10.22146/jpti.12231

Abstract

Fusarium wilt disease caused by Fusarium oxysporum fsp. cubense is the most important and destructive disease on banana. The pathogen is soil-borne and can survive in soil without the occurence of the host. The Vesicular Arbuscular Mychorriza (VAM) is a symbiotic associated fungi and plant roots. The VAM which infected plant can induce the resistance to pathogen. Banana seedlings obtained from tissue culture one free from pathogen. The seedling was inoculated with VAM to induce the resistance to Fusarium wilt disease. The result showed that the seedling inoculated with VAM can reduce diseases intensity of Fusarium wilt on banana.
Kajian Beberapa Jamur Entomopatogenik pada Ulat Daun Kubis Hijau, Plutella xylostella Tri Harjaka; Suryanti Suryanti
Jurnal Perlindungan Tanaman Indonesia Vol 8, No 2 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12362

Abstract

The use of entomopathogenic fungi for controlling Diamond Back Moth (DBM), Plutella xylostella L. still limited. Even though there are some entomopathogenic fungi could infect DBM. The aim of this research is to know the kind of entomopatogenic fungi on DBM to be used as biological control agent. Some isolates of  fungi were collected  from DBM infected by  the fungi  on field  in Central Java and  Yogyakarta. Fungi infecting DBM was  isolated, and cultured on Potato Dextrose Agar (PDA). Purification, identification and  infection tests were done to know  the potency of each fungal isolates. The results showed thatfive species of fungi infecting DBM, are Beauveria bassiana, Metarhizium anisopliae, Paecilomyces sp. Entomophthora sp.and Hirsutella sp.
Pemanfaatan Antibodi Monoklonal dalam I-ELISA untuk Deteksi Penyebab Penyakit Busuk Pucuk Kelapa (Phytophthora palmivora) Susamto Somowiyarjo; Daisy Prapto Sriyanti; Mulyadi Mulyadi; Suryanti Suryanti; Y. M.S. Maryudani; Bambang Hadisutrisno
Jurnal Perlindungan Tanaman Indonesia Vol 5, No 2 (1999)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12762

Abstract

Mice monoclonal antibodies (MCAs) against Phytophthora palmivora, Butl. (the pathogen of coconut bud rot) have been produced and tested. The selected hybridoma (Ab-PM.3) secreted monoclonal antibodies of IgM subclass and the titer in vitro of 10^2 - 1O^3, was employed to develop Indirect-Enzyme Linked lmmunosorbent Assay. This assay was able to differenciate coconut isolate of P. palmivora not only from cocoa and pepper isolate of the same species, but also from other fungus isolated from coconut bud rot diseased coconut (Fusarium sp., Colletotrichum sp., and Thielaviopsis sp.). This assay was able to differenciate P. pulmivora isolate from diseased coconut with those isolated from cocoa and pepper, and other fungus isolated from diseased coconut, thereby providing specific diagnostic tool for coconut bud rot which is needed for supporting the application of the concept of integrated pest mangement on coconut.
Co-Authors Arif Wibowo Bambang Hadisutrisno Christanti Sumardiyono Daisy Prapto Sriyanti Dwi Kiswanti Mulyadi Mulyadi Susamto Somowiyarjo Tri Harjaka Y. M.S. Maryudani Yufita Dwi Chinta