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All Journal Journal of Tropical Biodiversity and Biotechnology Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS)
Sukirno Sukirno
Faculty Of Biology, Gadjah Mada University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Other

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The Effectiveness of Red Spinach (Amaranthus tricolor L.) and Green Spinach (Amaranthus hybridus L.) Extracts for Bacillus thuringiensis var. kurstaki Protectant against UVB Radiation for the Control of Armyworm (Spodoptera litura Fab.) Siti Sumarmi; Mifta Arlinda; Sukirno Sukirno
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 2 (2020): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53004

Abstract

Spodoptera litura Fab. is an insect that damage cultivated plants in Indonesia. Efforts to control it can be done by using biological agents for example by using Bacillus thuringiensis kurstaki (Btk.). Unfortunately, the Btk. is easily degraded by UV radiation. This research aimed to study the effectiveness of red and green spinach as UVB protection for Btk. and to observe the pathogenicity of Btk. formulations against armyworm. Furthermore, the sublethal effect of Btk. against S. litura was investigated. The morphology of the endospore, protein crystal, and bacterium were observed under a contrast phase microscope. The extracts at 2% (w/v) were mixed with Btk. suspensions at 5 x 104, 5x105, and 5x106 (spores/ml), respectively. The formulations then exposed under Ultraviolet B (UVB) lights for 3, 6, and 9 hours then tested against the 3rd larval instar of armyworm. The larval mortality was observed daily and the analysis of variance was analyzed by one way anova. The sublethal effects of the treatment to the pupal and adult stages were observed when the moths emerge. The results showed that the larval mortality caused by  Btk. mixed with red spinach ranged from 11.7 to 26.7%. The sublethal effects of Btk. resulted in smaller sizes of pupae and imago, darker pupae, and wings abnormality of the adult stage, compared to any control treatment. The morphological observation of the bacteria showed that extracts gave UV protection against UVB. These results suggested that red and green spinach potentially can be used as a protectant for Btk. against UVB.
Pemanfaatan pekarangan dan limbah rumah tangga untuk budidaya lele sebagai upaya memenuhi pangan keluarga selama pandemi covid-19 Aprilia Sufi Subiastuti; Budi Setiadi Daryono; Sukirno Sukirno
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 5, No 1 (2022): Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v5i1.11323

Abstract

Pandemi Covid-19 menyebabkan perubahan kondisi sosial ekonomi masyarakat Indonesia. Sekitar 40% pekerja atau pelaku usaha mengalami perubahan pendapatan. Hal ini sedikit banyak mempengaruhi kemampuan masyarakat dalam memenuhi kebutuhan pangan keluarga. Pengabdian masyarakat ini bertujuan untuk memberikan edukasi kepada masyarakat untuk mengoptimalkan pemanfaatan pekarangan dan limbah rumah tangga untuk memenuhi kebutuhan pangan keluarga melalui budidaya lele. Pelaksanaan program dibagi ke dalam tiga tahap, yaitu pelatihan warga, pendampingan, dan evaluasi.Pelatihan warga meliputi pelatihan budidaya lele dan kangkung secara hidroponik menggunakan pot dan pelatihan pembuatan pakan lele dari limbah rumah tangga. Pendampingan dilakukan secara berkala setiap minggu hingga lele bisa dipanen. Evaluasi dilakukan di akhir kegiatan untuk mengetahui efektivitas dan potensi teknologi untuk dikomersilkan. Hasil dari program ini menunjukkan bahwa budidaya lele dan kangkung menggunakan pot dapat digunakan untuk memenuhi kebutuhan pangan keluarga dengan harga yang murah. Desain teknologi kolam portabel dari pot ini juga berpotensi untuk dikembangkan sebagai industri rumah tangga. Pakan lele dari limbah rumah tangga mampu menekan biaya pakan dalam budidaya lele hingga 20% dengan nilai rasio konversi pakan (FCR) sebesar 1.11 atau efisiens pakan hingga  90%. Pakan lele ini membantu pengurangan sampah sisa makanan dan sampah dapur hingga 100%.
Effectivity of Spodoptera littoralis Nucleopolyhedrovirus (SpliMNPV) and Natural Additives Mixtures against Spodoptera litura Fab. (Lepidoptera: Noctuidae) on Cabbage Plants Sukirno Sukirno; Bellia Alda Ayu Prasetya; Aryo Seto Pandu; Siti Sumarmi; Hari Purwanto; Ignatius Sudaryadi; Suparmin Suparmin; RC Hidayat Soesilohadi
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.71134

Abstract

Armyworm (Spodoptera litura Fab.) is one of the agricultural pests that can cause huge losses especially for Indonesian farmers because it is damaging various crops, especially cabbage (Brassica oleifera L.). Spodoptera littoralis nucleopolyhedrovirus (SpliMNPV) is one of the biological agent which is effective for the management of the Spodoptera litura. However, because of UV radiation it easily degraded when applied in the fields. This study was aimed to determine the effectivity of several indigenous plants for UV protectant of SpliMNPV for controlling armyworm at greenhouse scale. Extracts of 2% (w/v) of turmeric rhizome, red betel leaf, moringa leaf, and clove flower, were formulated with SpliMNPV and sprayed evenly onto two-month- old cabbages. The experiment used five replicates with six periods of sunlight exposures (0, 1, 3, 5, 7, and 15 days). A commercial product of deltamethrin was used as a comparison. The sprayed leaves were then used as a bioassay by using 25 individuals of one day old 1st larval instar by five replicates. The results showed that the turmeric additive was the most effective as a UV protectant and effectively prolonged the half-life of SpliMNPV to 4.12 days, while for clove, moringa leaf, and red betel was 2.48, 2.15, and 2.28 days, respectively.
Effectiveness of Liquid Organic Fertilizer Byproduct of Black Soldier Fly Maggot to the Growth of Mustard Plant (Brassica juncea L.) Laras Nur Janah; Anisa Azmi Nurrisky Apriliani; Afifah Nur Hidayah; Vania Adhelia; Sukirno Sukirno; Budi Setiadi Daryono
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.70530

Abstract

The need for consumption of mustard plant (Brassica juncea L.) has increased every year. One of the efforts to increase its production and quality by applications of inorganic and organic fertilizer. This study aimed to evaluate the productivity of mustard plants treated with liquid organic fertilizer, a by-product of black soldier fly (BSF), and inorganic fertilizer (NPK). Mustard plants were grown at Karanggayam Research Station, Caturtunggal, Depok, Sleman, Yogyakarta were treated with water as a control, NPK fertilizer, DoctoRS organic fertilizer at 0.05%, organic fertilizer A, and B at 0.1, 0.15, 0.20, and 0.25%. The effects of treatments to the phenotypic and the chlorophyll of the mustards were done after 2 weeks of treatments. The results showed that there were significant different on the stem height, number of leaves, leaf width, leaf length, and wet weight. These were in line with the total chlorophyll. Liquid organic fertilizer content analysis showed that DoctoRS liquid organic fertilizer and liquid organic fertilizer A were the most in accordance with the national standards for organic fertilizers on the parameters of pH, Mg, Ca, and TPC. 
Co-Authors Afifah Nur Hidayah Anisa Azmi Nurrisky Apriliani Aprilia Sufi Subiastuti Aryo Seto Pandu Bellia Alda Ayu Prasetya Budi Setiadi Daryono Budi Setiadi Daryono Hari Purwanto Ignatius Sudaryadi Laras Nur Janah Mifta Arlinda R.C. Hidayat Soesilohadi Siti Sumarmi Suparmin Suparmin Vania Adhelia