Article Per Year (5 Year)
p-Index From 2019 - 2024
1.116
P-Index
This Author published in this journals
All Journal Biogenesis: Jurnal Ilmiah Biologi Journal of Tropical Biodiversity and Biotechnology AGROMIX Berkala Ilmiah Biologi
Siti Sumarmi
Fakultas Biologi Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
The Effectiveness of Red Spinach (Amaranthus tricolor L.) and Green Spinach (Amaranthus hybridus L.) Extracts for Bacillus thuringiensis var. kurstaki Protectant against UVB Radiation for the Control of Armyworm (Spodoptera litura Fab.) Siti Sumarmi; Mifta Arlinda; Sukirno Sukirno
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 2 (2020): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53004

Abstract

Spodoptera litura Fab. is an insect that damage cultivated plants in Indonesia. Efforts to control it can be done by using biological agents for example by using Bacillus thuringiensis kurstaki (Btk.). Unfortunately, the Btk. is easily degraded by UV radiation. This research aimed to study the effectiveness of red and green spinach as UVB protection for Btk. and to observe the pathogenicity of Btk. formulations against armyworm. Furthermore, the sublethal effect of Btk. against S. litura was investigated. The morphology of the endospore, protein crystal, and bacterium were observed under a contrast phase microscope. The extracts at 2% (w/v) were mixed with Btk. suspensions at 5 x 104, 5x105, and 5x106 (spores/ml), respectively. The formulations then exposed under Ultraviolet B (UVB) lights for 3, 6, and 9 hours then tested against the 3rd larval instar of armyworm. The larval mortality was observed daily and the analysis of variance was analyzed by one way anova. The sublethal effects of the treatment to the pupal and adult stages were observed when the moths emerge. The results showed that the larval mortality caused by  Btk. mixed with red spinach ranged from 11.7 to 26.7%. The sublethal effects of Btk. resulted in smaller sizes of pupae and imago, darker pupae, and wings abnormality of the adult stage, compared to any control treatment. The morphological observation of the bacteria showed that extracts gave UV protection against UVB. These results suggested that red and green spinach potentially can be used as a protectant for Btk. against UVB.
Efek subletal campuran ekstrak daun srikaya (Annona squamosa L.) dan rimpang kunyit (Curcuma domestica Val.) terhadap larva Spodoptera litura F. Ramadhan Taufika; Siti Sumarmi; Setyo Andi Nugroho

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.008 KB) | DOI: 10.35891/agx.v11i1.1901

Abstract

Spodoptera litura (Lepidoptera: Noctuidae) larvae are polyphagous insects whose populations cause damage to various types of plantations, horticulture, and food so that control efforts need to be done. One way to control the environment of environmentally friendly S. litura larvae is to use a plant-based insecticide, a mixture of A. squamosa leaf extract and C. domestica rhizome (test extract appendix). The purpose of this study was to determine the subletal effect of the test extract mixture on S. litura larvae, namely the duration of larvae and pupae stage, pupae weight, morphology and imago viability, and the ability of female imago to lay eggs to produce offspring. The test was carried out by the method of feed dip into the test extract mixture. Data on pupae weight were analyzed with ANOVA, followed by Duncan's further test with a 5% confidence level using SPSS 21 program. The results showed that the test extract mixture extended the stages of larvae and pupae, reduced pupa weight, imago wings did not develop fully, reduced imago size, and did not imago able to produce eggs. The results of this study could then be further tested on a greenhouse scale and photochemical analysis of a test extract mixture to determine bioactive compounds that act as plant-based insecticides.
Pemeliharaan ulat grayak (Spodoptera litura Fabricius) (Lepidoptera: Noctuidae) menggunakan pakan buatan pada skala laboratorium Ramadhan Taufika; Siti Sumarmi; Dian Hartatie

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1105.998 KB) | DOI: 10.35891/agx.v13i1.2866

Abstract

Spodoptera litura is widely used as a test in a research controlling pests in the laboratory so it needs to be maintained to get uniform test insects in large numbers. Rearing of insects in laboratory can use natural food or artificial diets. The purpose of this study was to know the number and percentage of deaths in each phase, the alleged cause of death in each phase, and know the passed of rearing S. litura in laboratory using artificial diet. Rearing of insects is carried out in the Entomology laboratory, Faculty of Biology, Universitas Gadjah Mada. Artificial diet used is Poitout diet with modification. Data obtained from the results of rearing of S. liturain laboratory are then arranged in the form of life tables at the end of the phase, the alleged cause of death, and the percentage of deaths. The results showed the passed of rearing in laboratory using artificial diet was 74.8%. Larvae death by15.7%, pupae that did not develop into imago by 11.3%, imago death by 4.7%, and eggs that did not passed is 18.2%. Alleged causes of death in all phases add to the contamination of diet by fungi, larvae eaten by other larvae, temperature and the capacity of the rearing chamber that are not compatible with the breeding of insects.
Atlas moth (Attacus atlas Linnaeus, 1758) silk sericin protein: The effect of extraction methods and storage time on its content Hipny Alwandri; Sukirno Sukirno; Siti Sumarmi
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 1 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i1.24373

Abstract

.
Effectivity of Spodoptera littoralis Nucleopolyhedrovirus (SpliMNPV) and Natural Additives Mixtures against Spodoptera litura Fab. (Lepidoptera: Noctuidae) on Cabbage Plants Sukirno Sukirno; Bellia Alda Ayu Prasetya; Aryo Seto Pandu; Siti Sumarmi; Hari Purwanto; Ignatius Sudaryadi; Suparmin Suparmin; RC Hidayat Soesilohadi
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.71134

Abstract

Armyworm (Spodoptera litura Fab.) is one of the agricultural pests that can cause huge losses especially for Indonesian farmers because it is damaging various crops, especially cabbage (Brassica oleifera L.). Spodoptera littoralis nucleopolyhedrovirus (SpliMNPV) is one of the biological agent which is effective for the management of the Spodoptera litura. However, because of UV radiation it easily degraded when applied in the fields. This study was aimed to determine the effectivity of several indigenous plants for UV protectant of SpliMNPV for controlling armyworm at greenhouse scale. Extracts of 2% (w/v) of turmeric rhizome, red betel leaf, moringa leaf, and clove flower, were formulated with SpliMNPV and sprayed evenly onto two-month- old cabbages. The experiment used five replicates with six periods of sunlight exposures (0, 1, 3, 5, 7, and 15 days). A commercial product of deltamethrin was used as a comparison. The sprayed leaves were then used as a bioassay by using 25 individuals of one day old 1st larval instar by five replicates. The results showed that the turmeric additive was the most effective as a UV protectant and effectively prolonged the half-life of SpliMNPV to 4.12 days, while for clove, moringa leaf, and red betel was 2.48, 2.15, and 2.28 days, respectively.
Effectivity of Entomopathogen Fungus Metarhizium anisopliae (Metchnikoff) Sorokin to Mortality of Aedes aegypti Linnaeus, 1762 Larvae Amanda Novitasari; Agustina Citra Windianingsih; Thiwuk Leres Kinanti; Siti Sumarmi; Sukirno; Hidayat Soesilohadi
Berkala Ilmiah Biologi Vol 14 No 1 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i1.4774

Abstract

The Aedes aegypti mosquito is the vector that causes the spread of the Dengue virus that causes Dengue Hemorrhagic Fever. Breaking the chain of spread of Dengue fever has been done a lot, but it can cause negative impacts to environment. It is necessary to use other alternative solutions. One of them uses natural bioinsecticides from entomopathogenic fungi. The entomopathogenic fungus that has been widely developed as a bioinsecticide is Metarhizium anisopliae. Metarhizium anisopliae is a fungus which has larvicidal activity. Isolates of Metarhizium anisopliae were obtained from the Center for Forecasting Plant Pest Organisms, Karawang, West Java and Aedes aegypti larvae were obtained from Parasitology Laboratory, Faculty of Medicine, Public Health, and Nursing, UGM. The research was conducted from November 2021-April 2022 at the Entomology Laboratory, Faculty of Biology and the Parasitology Laboratory, Faculty of Medicine, Public Health, and Nursing, UGM, Yogyakarta. The method in this research is the culture and manufacture of a suspension of the fungus Metarhizium anisopliae with an average conidia density 0,9 x 107, 0,57 x 106, 1,175 x 105, 5,25 x 104, 3 x 104, 12,5 x 103 of conidia/mL, rearing of Aedes aegypti, bioassay, and data analysis using Probit Test with SPSS software version 23 to determine LC50. The effective concentration to kill 50% of larvae was 0.9 x 107 conidia/ml. There was no significant effect between each different concentration in killing Aedes aegypti larvae. The LC50 value obtained was 1.4 x 107. Metarhizium anisopliae was effectively to control Aedes aegypti larvae.
Co-Authors Agustina Citra Windianingsih Amanda Novitasari Aryo Seto Pandu Bellia Alda Ayu Prasetya Dian Hartatie Hari Purwanto Hidayat Soesilohadi Hipny Alwandri Ignatius Sudaryadi Mifta Arlinda R.C. Hidayat Soesilohadi Ramadhan Taufika Setyo Andi Nugroho SUKIRNO Sukirno Sukirno Sukirno Sukirno Suparmin Suparmin Thiwuk Leres Kinanti