Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal Jurnal AgroBiogen
Muhamad Herman
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian Jl. Tentara Pelajar 3A, Bogor 16111
Agriculture, Biological Sciences & Forestry

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Analisis Integrasi dan Segregasi Gen Ketahanan terhadap Hawar Daun pada Progeni F1 Hasil Persilangan Tanaman Kentang Transgenik dengan Non Transgenik Alberta Dinar Ambarwati; Agus Purwito; Muhamad Herman; S. M. Sumaraow; Hajrial Aswidinnoor
Jurnal AgroBiogen Vol 5, No 1 (2009): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n1.2009.p25-31

Abstract

Potato late blight, caused by Phytophthora infestans is one of the most devastating plant diseases. Potato yield losses due to this disease ranged from 47-100%. Frequent intervals and high rates of fungicide spray, currently practiced by potato growers to control the disease are expensive. Host resistance is an alternative control measure that is more economically and environmentally sustainable. Development of late blight resistant plants was conducted by crossing RB transgenic Katahdin SP904 andSP951 as male and two susceptible (Atlantic, Granola) varieties as female parents. F1 progenies were molecularly characterized for the integration of the RB transgene and evaluated for their segregations. Crossing data of Atlantic x transgenic Katahdin SP904 and SP951 produced 71 (57.72%)berries with average number of seeds per berry 139.58 and 83 (41.29%) berries with average number of seeds/berry 85.23, respectively. Granola x transgenic Katahdin SP904 and SP951 crosses gave higher results in terms of berry set (79.55 and 84.44%, respectively) than Atlantic x transgenic Katahdin crosses. A total of 554 F1 progenies were analyzed for thepresence of the RB PCR marker. An expected 619-bp and 840-bp band were amplified in the progenies that contain the RB gene. The RB gene was integrated in 65 (45.45%), 77 (47.83%), 47 (45.63%), and 71 (48.30%) F1 progenies of Atlantic x transgenic Katahdin SP904, Atlantic x transgenic Katahdin SP951, Granola x transgenic Katahdin SP904, andGranola x transgenic Katahdin SP951, respectively. Chisquare tests showed that all the four cross combinations followed a 1 : 1 segregation ratio.
Transformasi Gen Antisens ACC Oksidase pada Pepaya dengan Teknik Penembakan Partikel Diani Damayanti; Sudarsono Sudarsono; Ika Mariska; Muhamad Herman
Jurnal AgroBiogen Vol 5, No 1 (2009): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n1.2009.p32-38

Abstract

Papaya (Carica papaya L.) is a climacteric fruit that exhibit avery fast ripening rate. Ethylene controls the ripening eventin the papaya fruit. 1-aminocyclopropane-1-carbocxylic acid(ACC) oxidase gene encodes a specific enzyme for ethylenebiosynthesis. The gene had become a target for manipulationto make a gene construct of an antisense ACC oxidaseto regenerate transgenic papaya that has a characteristic ofdelayed ripening. The objective of the experiment is to engineer transgenic papaya that has a delayed ripening characteristic by transforming papaya with the antisense ACC oxidase gene through particle bombardment technique. The immature embryos of papaya variety Burungwere used for the explants. Antisense ACC oxidase and reporter (gus) genes were co-transformed to papaya calli. Four hundreds eighteen calli were bombarded by the antisense ACC oxidase gene. The transformation experiment resulted 25 putatives transgenic plants out of fifty plantsacclimatized in a greenhouse. Gus gene expression assay observed at 9 days after bombardment showed that the papaya explants bombarded twice at 9 cm shoot distance had 53.3% transformation rate of gus positive and 5.25 blue spots number in average. The results of PCR analysis showed that four out of 25 transgenic putative papaya plants (TR6, TR9, TR20, dan TR24), indicated a positive PCR of the antisense ACC oxidase gene with the amplified fragment DNA size of 800 base pair.
Co-Authors Agus Purwito Alberta Dinar Ambarwati Diani Damayanti HAJRIAL ASWIDINNOOR Ika Mariska S. M. Sumaraow Sudarsono Sudarsono