Andriana Kartikawati
Balai Penelitian Tanaman Rempah dan Obat Jl. Tentara Pelajar No. 3

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The Potency of Endophytic Bacteria Isolated From Red Ginger to Enhance Black Pepper Seedlings Growth Andriana Kartikawati; NFN Gusmaini
Buletin Penelitian Tanaman Rempah dan Obat Vol 29, No 1 (2018): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v29n1.2018.37-46

Abstract

Endophytic bacteria have many benefits such as phosphate solubilization, nitrogen  fixation, increasing mineral absorption as well as enhancing plant resistance against diseases. The research aimed to obtain endophytic bacteria potential to stimulate the growth of black pepper seedling. The first step was isolating endophytic bacteria from roots, rhizomes, and leaves of healthy red ginger. Characterization and selection were conducted by evaluating the capability on phosphate solubilization, nitrogen fixation, and indole acetic acid (IAA) hormone production of the isolated endophytic bacteria. There were 36 isolates consisted of 14 isolates from leaves, 9 isolates from roots and 13 isolates from rhizomes. Seventeen isolates were capable to dissolve phosphate, fix nitrogen and produce IAA or possessing those three characters, but only nine isolates were tested on pepper seedlings. The nine isolates were Ajc 1, Ajc 3, Ajc 7, Ajc 9, Rjc 1, Rjc 6, Djc 3, Djc 6, and Djc 11. Further evaluation of the nine isolates which were inoculated to pepper seedlings, indicated that Rjc1 isolate enhanced pepper seedlings height better than other isolates.
PEMANFAATAN PUPUK HAYATI (BIOFERTILIZER) PADA TANAMAN REMPAH DAN OBAT / Biofertilizer Utilization on Spices and Medicinal Plants Andriana Kartikawati; Octivia Trisilawati; Ireng Darwati
Perspektif Vol 16, No 1 (2017): Juni, 2017
Publisher : Puslitbang Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/psp.v16n1.2017.33-43

Abstract

ABSTRAK Peningkatan hasil produksi pertanian khususnya pada tanaman rempah dan obat lebih banyak menggunakan pupuk kimia. Hal tersebut dapat memberikan efek negatif bagi lingkungan, antara lain degradasi lahan baik secara fisik (degradasi struktur tanah), kimia dan biologi, serta polusi air tanah. Solusi yang diupayakan untuk menanggulangi dampak penggunaan pupuk kimia yaitu pemanfaatan mikroorganisme dalam pupuk hayati. Aplikasi pupuk hayati sudah dikembangkan secara luas pada tanaman rempah dan obat. Pupuk hayati menjaga lingkungan tanah yang kaya hara mikro dan makro melalui fiksasi nitrogen, pelarutan fosfor dan mineralisasi kalium, pelepasan zat pengatur tumbuh tanaman, produksi antibiotik dan biodegradasi bahan organik tanah. Mikroorganisme yang digunakan dalam pupuk hayati terdiri dari berbagai macam, seperti mikoriza, fungi dan bakteri, baik yang bersimbiosis dengan tanaman maupun yang hidup bebas di lingkungan.  Banyak penelitian yang telah dilakukan untuk mengetahui pengaruh pupuk hayati pada tanaman rempah dan obat. Hasil yang diperoleh menunjukkan bahwa pupuk hayati memberikan pengaruh positif pada berbagai tanaman rempah dan obat seperti lada, cengkeh, jahe, artemisia, ketumbar, panili, adas, dan lain-lain. Hal ini dapat dilihat pada peningkatan parameter pertumbuhan tanaman (tinggi, jumlah dan luas daun, perakaran), maupun hasil senyawa metabolit sekunder yang dihasilkan. Penggunaan pupuk hayati pada tanaman memberi dampak kesehatan manusia dan lingkungan.  ABSTRACT Increased agricultural production, especially in spices and medicinal plants utilize chemical fertilizers wildly. It could provide negative effect to the environment, including land degradation by physically (soil structure degradation), chemically and biologically, and also groundwater pollution. Attempted solutions to cope with the impact of chemical fertilizers utilization is by using beneficial microorganisms in the form of biofertilizer. Biofertilizer maintains soil environment having rich in micro and macro nutrients through N fixation, solubilize and mineralize phosphorus and potassium, release plant growth regulators, antibiotic production and biodegradation of soil organic matter. There are various kinds of microorganisms contain in biofertilizers, such as mycorrhiza, fungi and bacteria, which have mutual symbiotic with plants and also free-living microorganisms in their environment. The application of biological fertilizer has been developed extensively on spices and medicinal plants. Many studies have been conducted to find the effect of biofertilizer on spices and medicinal plants. The results showed that the usage of biofertilizers have a positive effect on various crops and medicinal spices such as pepper, clove, ginger, artemisia, coriander, vanilla, fennel, and others. It can be seen on the increase of plant growth and secondary metabolites content produced by these plants. 
The Potency of Endophytic Bacteria Isolated From Red Ginger to Enhance Black Pepper Seedlings Growth Andriana Kartikawati; NFN Gusmaini
Buletin Penelitian Tanaman Rempah dan Obat Vol 29, No 1 (2018): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v29n1.2018.37-46

Abstract

Endophytic bacteria have many benefits such as phosphate solubilization, nitrogen  fixation, increasing mineral absorption as well as enhancing plant resistance against diseases. The research aimed to obtain endophytic bacteria potential to stimulate the growth of black pepper seedling. The first step was isolating endophytic bacteria from roots, rhizomes, and leaves of healthy red ginger. Characterization and selection were conducted by evaluating the capability on phosphate solubilization, nitrogen fixation, and indole acetic acid (IAA) hormone production of the isolated endophytic bacteria. There were 36 isolates consisted of 14 isolates from leaves, 9 isolates from roots and 13 isolates from rhizomes. Seventeen isolates were capable to dissolve phosphate, fix nitrogen and produce IAA or possessing those three characters, but only nine isolates were tested on pepper seedlings. The nine isolates were Ajc 1, Ajc 3, Ajc 7, Ajc 9, Rjc 1, Rjc 6, Djc 3, Djc 6, and Djc 11. Further evaluation of the nine isolates which were inoculated to pepper seedlings, indicated that Rjc1 isolate enhanced pepper seedlings height better than other isolates.