Ni Nyoman Purwani
Department Of Health, Faculty Of Vocational Studies, Universitas Airlangga, Surabaya-Indonesia

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ENZIM: APLIKASI DI BIDANG KESEHATAN SEBAGAI AGEN TERAPI Ni Nyoman Purwani
QUANTUM: Jurnal Inovasi Pendidikan Sains Vol 9, No 2 (2018): Oktober 2018
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/quantum.v9i2.5574

Abstract

Enzim merupakan biokatalis untuk berbagai macam reaksi dan mengontrol semua proses metabolisme yang berlangsung pada tubuh manusia mulai dari hal sederhana seperti mengatur pencernaan sampai ke tingkat yang lebih komplek seperti pengaturan sistem kekebalan tubuh manusia. Ketiadaan enzim dapat menyebabkan keseimbangan proses metabolisme tubuh terganggu. Beberapa penelitian terbaru mengenai enzim telah mempelajari kemungkinan enzim untuk dapat mengatasi penyakit jantung , ganggunan pencernaan dan juga kanker. Namun demikian hanya sedikit informasi mengenai enzim-enzim yang telah digunakan untuk mengatasi berbagai jenis penyakit. Karena itu pada review ini akan dibahas jenis-jenis enzim dengan aplikasinya di bidang kesehatan.
ISOLASI DAN PENENTUAN INDEKSHIDROLISIS BAKTERI AMILOLITIK DARI TANAH SEDIMENT MANGROVE DI WONOREJO, SURABAYA Aliyah Siti Sundari; Ni Nyoman Purwani; Anita Kurniati
QUANTUM: Jurnal Inovasi Pendidikan Sains Vol 10, No 1 (2019): April 2019
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/quantum.v10i1.5879

Abstract

Mangrove sediment is a habitat for various bacteria, one of them is amylolytic bacteria which has the potential to produce amylase enzyme. Amylase enzyme has many benefits in industry, textiles and medical. The mangrove ecosystem area has special soil characteristics, which have the opportunity to have microorganism diversity, one of which is the mangrove ecosystem in the Wonorejo region, Surabaya. This study aims to obtain potential amylolytic bacteria from potential amylolytic isolates derived from mangrove sediment. Soil bacteria were isolated in Starch media for 2% agar and tested with Lugol’s Iodine reagents to measure their amylolytic index. Character isolates observed included colony morphology, Gram staining, and motility. Of the 27 isolates found there were 3 isolates with the highest index values in their activity, namely isolates A.7, A.27 and A.64. Characterization results showed that isolates A.7 and A.64 were Gram negative bacteria, and isolates A.27 were Gram positive bacteria. And the motility results for the three isolates were negative, with the results of a positive catalase test.
ANALYSIS OF THE INCIDENT RATE OF PEOPLE WITH A POTENTIAL RISK FOR HYPERTENTION AND DIABETES IN KENJERAN SUB-DISTRICT, SURABAYA Maya Septriana; Aliyah Siti Sundari; Diyantoro Diyantoro; Dwi Wahyu Indriati; Anita Kurniati; Ni Nyoman Purwani; Tofan Agung Eka Prasetya; Fadilatus Sukma Ika Noviarni; Shelly Wulandari; Myrna Adianti
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 1 No. 1 (2019): June 2019
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.389 KB) | DOI: 10.20473/dc.V1.I1.2019.11-15

Abstract

Background: The number of people with diabetes mellitus in Indonesia is increasing every year. The data in 2016 showed that 1 of 11 adults could have diabetes mellitus. Uncontrolled and untreated diabetes mellitus can cause complications. Objectives: This activity aimed to determine the group of age that have a potential risk of hypertension and diabetes so that it could minimize the prevalence of disease and optimize human productivity. Methods: (1) Counseling about hypertension and diabetes, (2) Screening the people with a potential risk of hypertension using tensimeter and diabetes prediction using point-of-care testing (POCT) and urine dipstick. Results: The data were collected from 75 respondents with characteristics about 85,33% female and 14,67% male. About 25 respondents (33,33%) were suspected of hypertension. Based on age group, it showed that the highest incident rate of hypertension was found in the age group of 41-60 years (41,86%), followed by the age group of 61-80 years (20%), 21-40 years (13,33%), and age under 20 years and above 80 years (0%). The majority of respondents had a normal glucose level(90,67%). Based on gender, there was 1 male respondent (9,09%)and5female respondents  (7,81%) having a glucose level higher than normal value (hyperglycemia). Conclusion:  The age group with the highest potential risk for hypertension was 41-60 years, while for diabetes was 61-80 years. This activity provided information to prevent the potential risk leading to hypertension and diabetes.
RAPID AND SPESIFIC DETECTION OF MYCOBACTERIUM TUBERCULOSIS USING POLYMERASE CHAIN REACTION Anita Kurniati; Desak Nyoman Surya Suameitra Dewi; Ni Nyoman Purwani
Journal of Vocational Health Studies Vol. 3 No. 2 (2019): November 2019 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1701.629 KB) | DOI: 10.20473/jvhs.V3.I2.2019.83-88

Abstract

Background: Tuberculosis (TB) is one of the major causes of health burden worldwide, especially in lower middle-income countries. TB is caused by Mycobacterium tuberculosis (MTB) and characterized by severe condition incuding coughing and fever. Purpose: To review the current methods for detection of TB using Polymerase Chain Reaction (PCR). Review: several studies have been done to give valuable insight into TB transmission, diagnosis, and treatment, however research  is constantly  needed  to decrease the incidence of eradicate TB. This infectious disease still give big health problem in all over the world by being second in causing high mortality rates after HIV/AIDS.  A specific, sensitive, rapid and cheap method for TB and other mycobacteria diagnosis in clinical specimen is a desperate needed in the laboratory diagnosis and hence management of tuberculosis. PCR as one of nucleic acid amplification assays have revolutionized MTB detection. Since it was first invented in fifteen years ago, it’s been through many developments. Conclusion: PCR  is one of the most specific and sensitive method currently available for TB diagnosis that can also detect in in all types of specimens obtained from TB patients.