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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Norman Arie Prayogo
Center of Maritime Bioscience, Jenderal Soedirman University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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MOLECULAR CLONING OF THE VITELLOGENIN GENE IN THE HARD-LIPPED BARB (Osteochillus hasseltii C.V) AND PHOTOPERIOD'S EFFECTS ON GENE EXPRESSION Norman Arie Prayogo; Asrul Sahri Siregar; Purnama Sukardi; Yasumasa Bessho
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 3 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.482 KB) | DOI: 10.11598/btb.2018.25.3.876

Abstract

Photoperiod affects fish reproduction as it regulates activities of the endocrine glands, which produce the hormones needed for geonadal growth and development, gametogenesis, and reproductive cycles. This study aimed to determine the effects of photoperiod on the hard-lipped barb's reproductive performance by exposing the fish to three photoperiod treatments (light hour: L, darks hour: D), namely 14L:10D (control), 8L:16D (short photoperiod) and 18L:6D (long photoperiod), with four aquaria, each containing 9 fish, serving as replicates. The fish were kept under these photoperiods for 8 weeks. Liver activity, the observable variable in the study, was evaluated by measuring vitellogenin gene expression. Normalized data were then subjected to ANOVA, followed by Tukey's range test. The hard-lipped barb's vitellogenin cDNA was found have a 1136 bp sequence and the vitellogenin precursors encoded cDNA comprising 378 amino acids. The vitellogenin gene in each experimental group saw a significant increase on average when exposed to longer photoperiods (P<0.05), and the highest levels of vitellogenin gene expression occurred under long photoperiods (LP, 18 h light:6 h dark). These results indicate that longer photoperiods stimulate and improve the hard-lipped barb's reproductive performance.
EFFECTS OF PHOTOPERIODS ON THE CLONED GTH GENES IN HARD-LIPPED BARB (Osteochillus hasseltii) Norman Arie Prayogo; Taufik Budi Pramono; Asrul Sahri Siregar; Purnama Sukardi; Masashi Kawaichi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 26 No. 3 (2019)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.3.1100

Abstract

Photoperiod is one factor that regulates the endocrine gland’s production of hormones necessary for gonadal growth and development, gametogenesis and reproductive cycles in fish reproduction. However, studies on the influence of photoperiod on tropical fishes are still limited. Hence, this study investigates the effects of photoperiod on the hard-lipped barb, particularly, the genetic expression of its cloned gonadotropin hormones I (GtH-I) and II (GtH-II) genes. Experiment was designed using three treatments; 14L: 10D (control), 8L: 16D (short photoperiods), and 18L: 6D (long photoperiods). Four aquariums with nine fishes/tank were used, serving as replicates. The fishes were kept under these different photoperiod lengths for eight weeks.  Pituitary activities were observed by measuring the GtH genetic expression.  The length of cDNA GtH-I was 222 bp, and the cDNA GtH-II was 354 bp. The GtH-I precursors encoded by cDNA consisted of 67 amino acids, including mature peptide. The level of GtH gene expression significantly increased as longer photoperiods were administered. The results indicated that photoperiod lengths affect the hard-lipped barb reproductive performance.
Co-Authors Asrul Sahri Siregar Asrul Sahri Siregar Masashi Kawaichi Purnama Sukardi Purnama Sukardi Taufik Budi Pramono Yasumasa Bessho