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All Journal IPTEK The Journal of Engineering Seminar Nasional Teknik Kimia Kejuangan I-Com : Indonesian Community Journal Sewagati Jurnal Teknik Kimia dan Lingkungan
Lailatul Qomariyah
Departemen Teknik Kimia Industri, Institut Teknologi Sepuluh Nopember, Kampus ITS, Keputih, Sukolilo, Surabaya 60111
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Materials Science & Nanotechnology Mechanical Engineering Public Health Social Sciences Other

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Journal : IPTEK The Journal of Engineering

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The Production of Gelatin from Snapper Scales (Lutjanus camphecanus Sp.) through Enzymatic Pretreatment Warlinda Eka Triastuti; Suprapto Suprapto; Elly Agustiani; Lailatul Qomariyah; Agung Subyakto; Treisnaning Widasgantri; Marchel Abednego Septa K
IPTEK The Journal of Engineering Vol 8, No 1 (2022)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23378557.v8i1.a12860

Abstract

Fish wastes such as fish bones and scales have a potential to be used as a gelatin source. It can be used as an alternative material to replace pork or bovine which ordinarily conflicted with religion issue. Gelatin from fish has different characteristics compared with mammalian source. It has several advantageous properties such as high amount of hydrophobic amino acids, less proline and hydroxy proline, lower gelling ability, and melting point. In this study, gelatin was produced from fish waste especially snapper scales using the bromelain enzyme at the pre-treatment stage to improve its characteristic. The snapper scales soaking in hot water at 80⁰C for about 30 minutes to remove the fat which attached the scales. A pre-treatment has been conducted using bromelain enzyme solution at various concentrations of 1%, 2%, 3%, 4% and 5% for 6 hours to remove non-collagenous proteins and loosen the bonds of the fish scales. The next step is demineralization using a 15% concentration of citric acid solution for 3 days. The ossein formed in the demineralization stage was then hydrolyzed at 50⁰C and 70⁰C for 9 hours with stirring at 300 rpm. The best gelatin yield was obtained from the pretreatment of bromelain enzyme solution with a concentration of 3% at a hydrolysis temperature of 70⁰C with a yield of 9.13%, pH value of 3.86, water content of 8%, ash content of 0.80%. FTIR spectra shows the presence of functional groups correspond to gelatin formation, such as carbon, hydrogen, hydroxyl group (O-H), carbonyl group (C=O), amine group (N-H) and alkene group (C=C). The heavy metal content test showed that the gelatin contained no heavy metal content of lead (Pb), mercury (Hg), cadmium (Cd) and arsenic (As).
Co-Authors Achmad Dwitama Karisma Agung Subyakto Agus Surono Agustiani, Elly Ahmad Nezard Dhiaulhaq Altway, Saidah Danawati Hari Prajitno Faizal Johan Atletiko Irfan Ramadhitya Marchel Abednego Septa K Rachmaniah, Orchidea Rivai Wardhani Sultan Hendra Mahardi Kalloka Sulthan Rabbani Suprapto Suprapto Suprapto Suprapto Treisnaning Widasgantri Triastuti, Warlinda Eka Yuni Kurniati Yuni Kurniati Zel Andesra Zuchrillah, Daril Ridho