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All Journal BERITA BIOLOGI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Arbayah H Siregar
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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POLA PRODUKSI AJMALISIN DARIKULTUR AGREGAT SEL Catharanthus roseus (L) G Don. DALAM BIOREAKTOR Aida Muspiah; Rizkita Rahmi Esyanti; Arbayah H Siregar; Erman Tritama
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1212

Abstract

A research has been conducted to optimize the rate of aeration and initial cell aggregates weight in the production of ajmalicine in Catharanthus roseus cell culture in bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.5 x 10"6 M NAA ('naphthalene acetic acid') and 10s M BAP ('benzyl amino purine'). Cell aggregates were subcultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with the rate of 0.2460 L/min and 0,3405 L/min, respectively. The pattern of ajmalicine production in bioreactor were observed every 3 days for 24 days.Qualitative and quantitative analysis were conducted using HPLC connected to Cromatopac CL-7APlus. The results showed that the cell aggregates and medium contain ajmalicine. The highest concentration was obtained in combination of 30 g/fw and 0.3405 L/min aeration compare to 20 g/fw - 0,246 L/min, 20 g/fw- 0,3405 L/min, as well as 30 g/fw - 0,2460 L/min. The highest ajmalicine content in cell aggregates was obtained on the 12"d days (79.23ug/g) whilst in medium was obtained in the 18th days (981.15 ug/L).
PENGARUH PEMBERIAN ELISITOR EKSTRAK KHAMIR Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN AJMALISIN DALAM KULTUR AGREGAT SEL Catharanthus roseus (L.) G. Don. Jujun Ratnasari; Arbayah H Siregar; Rizkita RE
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1118

Abstract

There were many ways to obtain high production of secondary metabolites in plant tissue culture; among the other is elicitation.An experiment to study the effect of elicitor derived from Saccharomyces cerevisiae Hansen extract on ajmalicine content in cell aggregates culture of Catharanthus roseus (L.) G.Don, has been conducted. The media used for callus induction and cell aggregates culture were Zenk 6 5(1977) with addition of 2.5 x 10" M Naphthalene Acetic Acid (NAA) and 10' M 6-Benzilaminopurine (BAP).The cell aggregates culturewas subcultured three times and then elicitated with elicitor derived from autoclaved 5.cerevisiae extract at concentrations 0.5, 1.0, 2.5%,and harvested at 18, 24,and 36 hours after elicitation.The ajmalicine was analyzed qualitatively and quantitatively by using High Pressure Liquid Chromatography (HPLC) connected to chromatopack CR-7A Plus. The cell aggregates of C. roseus culture produced ajmalicine both in the cells and the media.The result of elicitation showed that ajmalicine content was influenced significantly by concentration and harvesting time. The highest ajmalicine content in the cell aggregates was 25.288 ± 0.102 jig/g dw, whilst that in media was 524.600 ± 0.566 \\ML. The optimum concentration of S. cerevisiae extract was 0.5%, and the best harvesting time was 24 hours.
PENGARUH PEMBERIAN EKSTRAK Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN GOSIPOL PADA KULTUR KALUS Gossypium hirsutum L. Rama Riana Sitinjak; Arbayah H Siregar; Rizkita RE
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i2.1133

Abstract

This study concentrated on the effect of Saccharomyces cerevisiae Hansen extract on gosipol content of Gossypium hirsutum L callus cultures. There were many ways commonly used to obtain high secondary metabolites product by using tissue culture,i.e. elicitation.The objective of this study was to increase the production ofgossypol on G. hirsutum callus cultures using elicitor derived from S. cerevisiae.The callus grew optimally and produced gossypol on Linsmaierand Skoog (LS) medium with the addition of 10 M NAA and 10 M 2,4-D. Callus derived from cotyledon was subcultured five times.Callus was then elicited with elicitor derived from autoclaved S. cerevisiae and concentration of elicitor tested were 0.5, 1.0 and 2.5% (b/v). The harvesting times were 0, 2, 4, dan 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively by using High Performance Liquid Chromatography (HPLC).Gossypol content was influenced significantly by concentration of elicitor and harvesting time.The maximum ofgossypol content was obtained on the 4 day after elicitation i.e. 469.233 ± 2.332 pg/g dry weight in the elicited callus of G. hirsutum.
Pengaruh Elisitasi dengan Verticillium dahliae Kleb dan Rhizoctonia Solani Kuhn terhadap kandungan Gosipol dalam kalus Gossypium hirsutum L pada beberapa tingkat Subkultur Suci Rahayu; Rizkita Rachmi Esyanti; Arbayah H Siregar
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.140

Abstract

An experiment on gossypol content hasbeen conducted on callus derived from Gossypium hirsutum L. subcultures 5, 6, and 7 after elicitation with Verticillium dahliae Kleb and Rhizoctonia solani Kuhn. Callus was cultured on solid Linsmaier and Skoog  (LS)  medium  supplemented  with 10 –5 M NAA and 10 –6 M 2,4-D, and subcultured for 5, 6, and 7 times. Callus was then elicited with 40  μg dry weight /ml homogenate of fungi derived from V. dahliae and R. solani, and harvested 0, 2, 4, and 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively using High Performance Liquid Chromatography (HPLC). The maximum gossypol content elicited with R. solani was obtained on callus subculture 5, which was harvested on 4 days after elicitation, i.e. 177,995 ±  0,248 μg/g dry weight,  whilst that with V. dahliae  was obtained on subculture 5, harvested four days after elicitation, i.e.108,021± 0,507 μg/g dry weight. The gossypol content of control callus on subculture 5, 6, and 7 was 37,885 ± 0,779 μg/g dry weight  23,170 ±  0,003 μg/g dry weight, 12,284 ± 0,221 μg/g dry weight, respectively. The subculture level, elicitor type, and harvesting time gave significant effect on gossypol content of G. hirsutum callus culture.
Co-Authors AIDA MUSPIAH Erman Tritama Jujun Ratnasari Rama Riana Sitinjak Rizkita Rachmi Esyanti Rizkita Rahmi Esyanti Rizkita RE Suci Rahayu