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All Journal HAYATI Journal of Biosciences Agroland : Jurnal Ilmu-Ilmu Pertanian Jurnal Matematika & Sains Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Rizkita Rachmi Esyanti
Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Mathematics

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Pengaruh Pemberian Elisitor Homogenat Jamur Pythium aphanidermatum (Edson) Fitzp. terhadap Kandungan Ajmalisin dalam Kultur Akar Catharantus roseus (L) G. Don Mukarlina Mukarlina; Rizkita Rachmi Esyanti; Arbayah Hamonangan Siregar
Jurnal Matematika & Sains Vol 11, No 2 (2006)
Publisher : Institut Teknologi Bandung

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Abstract

An experiment to study the effect of elicitor from Pythium aphanidermatum (Edson) Fitzp. on ajmalicine content of Catharanthus roseus (L) G. Don. root culture has been conducted. The C. roseus root culture in Zenk medium (1977) with the the addition of 10-7 M of napthalene acetic acid (NAA) and 10-7 M of benzylaminopurine (BAP) as growth factors was elicited with autoclaved P. aphanidermatum homogenate at concentrations of 0.05, 0.5, 1.0 and 50 mg DW/mL, then harvested at 18, 24, 36, 48, and 72 h after elicitation. The ajmalicine content was analyzed qualitatively and quantitatively by HPLC (High Pressure Liquid Chromatography) connected to chromatopack CR-7A plus. The result showed that ajmalicine content was influenced significantly by the concentration of elicitor and harvesting time. The highest ajmalicine content on the roots i.e. 165.6 ± 1.1 μg/g DW with the increment percentage of 181,6% was obtained by addition of 5.0 mg DW/mL of elicitor for 24 h, while on the media, the highest ajmalicine content i.e. 291.5 ± 0.9 mg DW/mL with the increment percentage of 306,5% was obtained by addition of 5.0 mg DW/mL elicitor for 36 hours. These results indicated that elicitation might be responsible for the higher ajmalicine content in the media compared to its accumulation in the root.
Pengaruh Triptofan pada Pertumbuhan dan Kandungan Katarantin dari Kalus Catharanthus roseus Dingse Pandiangan; Dennie Heroike Rompas; Henry Fonda Aritonang; Rizkita Rachmi Esyanti; Erly Marwan
Jurnal Matematika & Sains Vol 11, No 4 (2006)
Publisher : Institut Teknologi Bandung

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Research on the growth and catharanthine content of Catharanthus roseus callus which were treated with tryptophan had been done. Wet and dry weight of Catharanthus roseus callus decreased when treated with 100, 125 and 225 mg/L tryptophan, but callus treated with 150, 175 and 200 mg/L tryptophan grew better. Catharanthine contents of the kontrol callus was 423,22 µg/g dw. The treatment calli contains 485,00, 588,32, 875,10, 905,26, 784,10 and 950,54 µg/g dw of catharanthine respectively. The optimal tryptophan treatment for callus growth and catharanthine production was 175 mg/L tryptophan with a 113,90 %. increase of catharanthine content.
Effect of 2,4-D on Indirect Somatic Embryogenesis and Surface Structural Changes in Garlic (Allium sativum L.) cv. Lumbu Hijau Totik Sri Mariani; Hiroshi Miyake; Rizkita Rachmi Esyanti; Ida Nurwendah
Jurnal Matematika & Sains Vol 8, No 4 (2003)
Publisher : Institut Teknologi Bandung

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A study concerning the effect of 2,4-D on indirect somatic embryogenesis and surface structural changes in garlic (Allium sativum L.) cv. Lumbu Hijau was conducted. The purposes of this study were to evaluate the effect of 2,4-D on the induction of somatic embryogenesis and to observe the developmental stage of somatic embryo as well as the surface structural changes of somatic embryo in garlic. Root tips explants were cultured on embryogenic callus induction medium (ECIM). Somatic embryos were then transferred to the embryo maturation medium (EMM), desiccated and subsequently transferred to the repetitive somatic embryogenesis medium (RSEM). Observation by dissecting microscope showed that embryogenic callus and somatic embryo was formed at ECIM containing 0.1 μM 2,4-D. At EMM containing 0.01 μM 2,4-D somatic embryo developed into mature somatic embryo. Somatic embryo underwent repetitive somatic embryogenesis that consisted of globular and mature somatic embryo at RSEM, without plant growth regulator. Observation by scanning electron microscopy showed that the development al stages of garlic somatic embryo consisted of proembryo, transition phase from proembryo to globular, globular, mature embryo and single cotyledon stages. Effect of 2,4-D on the cell surface structure could be seen on each somatic embryo developmental stage. In ECIM containing 0,1 μM 2,4-D, proembryo, transition embryo and globular embryo were formed. The surface of proembryo was smooth whereas fibrillar material as cellulose microfibril, was observed during the transition from proembryo to globular stage. The surface of globular stage was entirely covered by a new cell wall. Subsequently, in 0.01 μM 2,4-D, mature embryo and single cotyledon were formed, which their cell wall surface was stable. From this study, it could be concluded that the optimum concentration of 2,4-D for induction of embryogenic callus and initiation of somatic embryo was 0.1 μM. Decreasing of 2,4-D concentration to 0.01 μM resulted in the development of globular embryo to mature and single cotyledon stage. Repetitive somatic embryogenesis occurred in RSEM medium without plant growth regulator. Based on this result it can be concluded that each somatic embryo developmental stage had a typical characteristic of surface structure.
Production Pattern of Ajmalicine in Catharanthus roseus (L.) G. Don. Cell Aggregates Culture in the Airlift Bioreactor RIZKITA RACHMI ESYANTI; AIDA MUSPIAH
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.025 KB) | DOI: 10.4308/hjb.13.4.161

Abstract

A research has been conducted to optimize the rate of aeration and initial weight of cell aggregates in the production of ajmalicine in Catharanthus roseus cell culture in airlift bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.50 x 10-6 M naphthalene acetic acid (NAA) and 10-5 M benzyl amino purine (BAP). Cell aggregates were sub-cultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with the rate of 0.25 l min-1 and 0.34 l min-1, respectively. The pattern of ajmalicine production in bioreactor were observed in every three days within 24 days. Qualitative and quantitative analysis were conducted using HPLC connected to Cromatopac CL-7A Plus. The results showed that the cell aggregates and medium contain ajmalicine. The highest concentration was obtained in combination of 30 g/fw and 0.34 l min-1 aeration compare to 20 g/fw - 0.25 l min-1, 20 g/fw - 0.34 l min-1, as well as 30 g/fw – 0.25 l min-1/sup>. The highest ajmalicine content in cell aggregates was obtained on the 12 days (79.23 µg g-) whilst in medium was obtained in the 18thsup> days (981.15 µg l-). Key words: ajmalicine, cell aggregates culture, C. roseus, airlift bioreactor
Pengaruh Elisitasi dengan Verticillium dahliae Kleb dan Rhizoctonia Solani Kuhn terhadap kandungan Gosipol dalam kalus Gossypium hirsutum L pada beberapa tingkat Subkultur Suci Rahayu; Rizkita Rachmi Esyanti; Arbayah H Siregar
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.140

Abstract

An experiment on gossypol content hasbeen conducted on callus derived from Gossypium hirsutum L. subcultures 5, 6, and 7 after elicitation with Verticillium dahliae Kleb and Rhizoctonia solani Kuhn. Callus was cultured on solid Linsmaier and Skoog  (LS)  medium  supplemented  with 10 –5 M NAA and 10 –6 M 2,4-D, and subcultured for 5, 6, and 7 times. Callus was then elicited with 40  μg dry weight /ml homogenate of fungi derived from V. dahliae and R. solani, and harvested 0, 2, 4, and 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively using High Performance Liquid Chromatography (HPLC). The maximum gossypol content elicited with R. solani was obtained on callus subculture 5, which was harvested on 4 days after elicitation, i.e. 177,995 ±  0,248 μg/g dry weight,  whilst that with V. dahliae  was obtained on subculture 5, harvested four days after elicitation, i.e.108,021± 0,507 μg/g dry weight. The gossypol content of control callus on subculture 5, 6, and 7 was 37,885 ± 0,779 μg/g dry weight  23,170 ±  0,003 μg/g dry weight, 12,284 ± 0,221 μg/g dry weight, respectively. The subculture level, elicitor type, and harvesting time gave significant effect on gossypol content of G. hirsutum callus culture.
ANTAGONISITAS DAN EFEKTIVITAS Trichoderma sp DALAM MENEKAN PERKEMBANGAN Phytophthora palmivora PADA BUAH KAKAO Umrah Umrah; Tjandra Anggraini; Rizkita Rachmi Esyanti; I Nyoman P. Aryantha
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 16, No 1 (2009)
Publisher : Universitas Tadulako

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Abstract

The purpose of the study was to select several Trichoderma sp based on its sporalation capabilities, and antagonistic character, and effectiveness to control P. palmivora on cocoa pod.  The study consisted of two stages. In the first stage, a Completely Randomised design was used which consisted of seven treatments with three replicates: T. viridae, collection of  Laboratory of Plant Pest and Diseases of Bogor Institute of Agriculture (T-A), T harzianum, collection of Laboratory of Plant Pest and Diseases of Bogor Institute of Agriculture (T-B), T harzianum,  collection of  Coffee and Cacao Research centre of Jember (T-C), T koningii, collection of  Coffee and Cacao Research Centre of Jember (T-D), T. Trichoderma sp 1, collection of Laboratory of Microbiology  Life Sciences Centre of Bandung Institute of Technology    (T-E), Trichoderma sp 2, collection of Laboratory of Microbiology  Life Sciences Centre of Bandung Institute of Technology (T-F) and Trichoderma sp, collection of Laboratory of Plant Pest and Diseases University of Tadulako (T-G). Parameters measured were sporulation capability of Trichoderma sp, percentage of antagonisticity of Trichoderma sp towards in vitro P. palmivora . The second stage of the study was to determine the effectiveness of Trichoderma antagonisticity, either individual or mixed cultures in controlling the development of  P. palmivora on cocoa pod. The study was designed with seven treatments with three replicates:  T-D, T-E, T-G, T-DE  (mixed culture of T-D and T-E),  T-DG  (mixed culture of T-D and T-G), T-EG ( mixed culture of T-E and T-G), T-DEG (mixed culture of  T-D, T-E, and T-G). The results of the study showed that at in vitro condition the kinds of Trichoderma sp which had the highest antagonisticity on P. palmivora were  Trichoderma sp (T-G, T-E and T-D). They were effective to control P. palmivora on cocoo pod either in individual or mixed cultures.  However, T-G will be further developed due to its highest effectiveness as a biological control compared to the others.
Co-Authors AIDA MUSPIAH Anak Agung Istri Sri Wiadnyani Arbayah H Siregar Arbayah Hamonangan Siregar Dennie Heroike Rompas Dingse Pandiangan Erly Marwan Henry Aritonang Hiroshi Miyake I Nyoman P. Aryantha Ida Nurwendah Suci Rahayu Tjandra Anggraini Totik Sri Mariani Umrah Umrah