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All Journal Indonesia Chimica Acta Molekul: Jurnal Ilmiah Kimia
Seniwati Dali
Hasanuddin University
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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UTILIZATION OF ETHYL ACETATE FRACTION KETAPANG LEAF (Terminalia catappa) AS BIOREDUCTOR IN SYNTHESIS GOLD NANOPARTICLES AND ANALYSIS ANTIBACTERIAL PROPERTIES Sarwina Hafid; Muhammad Zakir; Seniwati Dali
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 8 No 2 - December 2015
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v8i2.2470

Abstract

Gold nanoparticles was synthesized by bioreduction method using ethyl acetate fraction of ketapang leaf (Terminalia catappa). Nanoparticles formed were characterized using spectrophotometer UV-Vis, Fourier Transform Infra Red (FTIR) and X-Ray Diffraction (XRD). In the   analysis   of   spectrophotometer   UV-Vis   gold   nanoparticles   with   a   precursor   solution concentration of 0.5 mM and 1 mM each has an absorption between 533.5-540 nm and 539-587 nm. Analysis of functional groups of gold nanoparticles was analyzed by Fourier Transform Infra Red (FTIR). Crystal system of gold nanoparticles is cubic with nanoparticles size of 17.13 nm. The evaluation of test antibacterial activity was performed using several bacteria test such as Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. Gold nanoparticles can not inhibit the activity of a test bacterium Staphylococcus aureus, Escherichia coli, and Bacillus subtilis so that differences in the concentration of gold nanoparticles did not affect the inhibition of bacteria.
Production and Characterization of Collagenase From Bacillus sp. 6-2 Isolated From Fish Liquid Waste Sartika Danial; Hasnah Natsir; Seniwati Dali; Leliani Leliani
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 12, No.1: June 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v12i1.5924

Abstract

Collagenases are enzyme that are able to hydrolyze native collagen into fragment collagen peptides. Collagenases and its hydrolysis products have received tremendous attention in medical and industrial applications. The present study was conducted to isolate and identify new collagenase producing bacteria from fish liquid waste, then produce and characterize collagenase. A total of 7 isolate from fish liquid waste were screened on selective medium containg 2 % collagen and its activity was confirmed by the formation of clear zone. Isolat 6-2 was positif as collagenase producer and identified as Bacillus sp. 6-2 by morphological and biochemical characteristics. The optimum fermentation time of enzyme was investigated. Collagenase crude extract was characterized by the effect of pH, temperature, and metal ions. Isolat 62 optimally produced collagenase enzyme after 30 h of incubation with activity of   0.072 U/mL and protein content of 3.768 mg/mL. The optimum pH and temperature were 7.0 and 40 oC, respectively. The enzyme was activated by 1 mM Ca2+and  Mg2+, and inhibited by   1 mM  Zn2+ and Co2+. Collagenase from Bacillus sp. 6-2 may have potentials for medical and industrial applications.
Collagen Extraction from Bone of Lutjanus sp. and Toxicity Assay Leliani Ramli; Hasnah Natsir; Seniwati Dali; Sartika Danial
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 12, No.1: June 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v12i1.5925

Abstract

Indonesia is a maritime country with the potential fisheries resources. However, utilization only revolves around the meat, while other parts have not been used optimally, especially fish bones which have the potential to produce collagen, so it needs to be developed. The aims of this study ware extracted collagen from bone of Lutjanus sp. and determine its the Anticancer activity. The collagen was extracted by using hydroextraction method and identification by FTIR. The initial screening anticancer activity was done by using Brine Shrimp Lethality Test (BSLT) method for toxicity assay. The results showed that the yield of collagen was 4.535% with protein concentration was 8,815 mg/mL. Identified collagen from spectrum of amide A, B, I, II, and III at 3421.72; 2926.01; 1651.07; 1541.12; 1240.23 cm-1. The toxicity test was shown in LC50 values of  8,760 μg/mL. The collagen from Lutjanus sp. bone can be used as natural anticancer agent.
Isolation and Identification of Cellulolytic Bacteria from Gut of Horn Beetle Larvae (Oryctes rhinoceros L.) Riskawati Riskawati; Hasnah Natsir; Seniwati Dali; Maswati Baharuddin
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6848

Abstract

The horn beetle larvae (Oryctes rhinoceros L.) contain symbiotic bacteria that are used to digest and degrade cellulose as food so that it has the potential to produce cellulase enzymes. This study aims to isolate, characterize and identify microbial symbionts from horn beetle larvae that have the potential to produce cellulase enzymes.The methods in this study include morphology and physiology identification of bacteria, qualitative and quantitative activity tests and species determination using 16S rRNA sequencing technique. Based on the results of morphological observations, five bacterial isolates were taken which has the potential as a cellulase producer is indicated by the presence of a clear zone that is produced when a qualitative test is carried out using congo red staining with different cellulolytic indices. Based on the quantitative bacterial activity test using UV-Vis, the highest activity was found in PES3 isolates at 1.62 x 10-2 and PES5 at 1.61 x 10-2. Species determination results found that PES3 isolates belonged to the genus Acinetobacter and PES5 belonged to Pseudomonas. In addition to the isolates obtained for the environment and the industrial sector, cellulolytic bacteria can provide added value such as hydrolyze cellulose waste into alternative fuels.
Isolation and Molecular Identification of Amylolytic Bacteria from Oryctes rhinoceros L. Larvae Decomposing Empty Palm Oil Fruit Bunches Sahriani Uto; Rugaiyah Arfah; Seniwati Dali; Maswati Baharuddin
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6957

Abstract

Oryctes rhinoceros L. is an organism that helps the decomposition of oil palm empty fruit bunches (OPEFB). In the larvae's intestines, there are symbiotic bacteria that are used in the process of food degradation in the digestive system, one of which is amylolytic bacteria. This study aims to isolate and molecular identify amylolytic bacteria that produce amylase enzymes from horn beetle larvae. The techniques are used to screen and isolate bacteria from horn beetle larvae. Bacterial identification was accomplished by microscopically identifying amylase-producing bacterial isolates, performing biochemical tests on selected bacterial isolates, quantifying amylase enzyme activity, and molecularly identifying 16S rRNA. The results of screening and bacterial isolation obtained five isolates. The largest amylolytic bacterial colony index value was obtained in the EA3 isolate, which was 1.370 mm. Bacterial isolates with the highest activity were found in isolates coded EA1 and EA2, namely 0.049 U/mL and 0.0479 U/mL. According to the findings of 16S rRNA molecular identification, isolates EA1 and EA2 had similarities with the bacteria Ochrobactrum sp. and Pseudomonas mendocina.
Co-Authors Hasnah Natsir Leliani Leliani Leliani Ramli Maswati Baharuddin Maswati Baharuddin Muhammad Zakir Riskawati Riskawati Rugaiyah Arfah Sahriani Uto Sartika Danial Sarwina Hafid