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All Journal Indonesia Chimica Acta
Leliani Ramli
Hasanuddin University
Chemical Engineering, Chemistry & Bioengineering

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Collagen Extraction from Bone of Lutjanus sp. and Toxicity Assay Leliani Ramli; Hasnah Natsir; Seniwati Dali; Sartika Danial
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 12, No.1: June 2019
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v12i1.5925

Abstract

Indonesia is a maritime country with the potential fisheries resources. However, utilization only revolves around the meat, while other parts have not been used optimally, especially fish bones which have the potential to produce collagen, so it needs to be developed. The aims of this study ware extracted collagen from bone of Lutjanus sp. and determine its the Anticancer activity. The collagen was extracted by using hydroextraction method and identification by FTIR. The initial screening anticancer activity was done by using Brine Shrimp Lethality Test (BSLT) method for toxicity assay. The results showed that the yield of collagen was 4.535% with protein concentration was 8,815 mg/mL. Identified collagen from spectrum of amide A, B, I, II, and III at 3421.72; 2926.01; 1651.07; 1541.12; 1240.23 cm-1. The toxicity test was shown in LC50 values of  8,760 μg/mL. The collagen from Lutjanus sp. bone can be used as natural anticancer agent.
Co-Authors Hasnah Natsir Sartika Danial Seniwati Dali