Indonesia Chimica Acta
Volume 12, No.1: June 2019

Collagen Extraction from Bone of Lutjanus sp. and Toxicity Assay

Leliani Ramli (Hasanuddin University)
Hasnah Natsir (Hasanuddin University)
Seniwati Dali (Hasanuddin University)
Sartika Danial (Hasanuddin University)



Article Info

Publish Date
30 May 2019

Abstract

Indonesia is a maritime country with the potential fisheries resources. However, utilization only revolves around the meat, while other parts have not been used optimally, especially fish bones which have the potential to produce collagen, so it needs to be developed. The aims of this study ware extracted collagen from bone of Lutjanus sp. and determine its the Anticancer activity. The collagen was extracted by using hydroextraction method and identification by FTIR. The initial screening anticancer activity was done by using Brine Shrimp Lethality Test (BSLT) method for toxicity assay. The results showed that the yield of collagen was 4.535% with protein concentration was 8,815 mg/mL. Identified collagen from spectrum of amide A, B, I, II, and III at 3421.72; 2926.01; 1651.07; 1541.12; 1240.23 cm-1. The toxicity test was shown in LC50 values of  8,760 μg/mL. The collagen from Lutjanus sp. bone can be used as natural anticancer agent.

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Journal Info

Abbrev

ica

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering

Description

Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) is a peer-reviewed research journal that is devoted to the dissemination of new and original knowledge in all branches of chemistry. The result of research and development in the fields of chemistry in both experimental and theory/ computation, ...