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All Journal Jurnal Ilmu Dasar Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Ilmiah Inovasi
Netty Ermawati
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Agriculture, Biological Sciences & Forestry Control & Systems Engineering Decision Sciences, Operations Research & Management Education Mathematics Other

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Isolation and Characterization of Gene Encoded Tonoplast Intrinsic Proteins from Halophyte Plant Salicornia herbacea Netty Ermawati
Jurnal ILMU DASAR Vol 12 No 1 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1326.445 KB)

Abstract

Salicornia herbacea is a succulent halophyte plant which grows optimally under 300 mM NaCl. These plants may have acquired specific genes that are essential for tolerating salt. To obtain insight into the comprehensive molecular characteristics related to the salt tolerance mechanism, we performed a screening of salt-inducible genes in S. herbacea shoots by differential display. A comparative analysis of gene expression between control and salt-stressed conditions led to the detection of 6 cDNA clones induced by salt. Sequence analysis and database searching revealed that all of the clones have homology with tonoplast intrinsic proteins. We designated them as ShTIP1 to ShTIP6 (S. herbacea Tonoplast Intrinsic Proteins). One the identified genes, ShTIP6 showed higher induced under salt stress compared to other ShTIP genes. In this study, we characterized the expression of ShTIP6 in different organs and stresses.
KEMAMPUAN KOMPETISI RELATIVE TEBU-PRG EVENT-5 TERHADAP GULMA DAUN SEMPIT DAN DAUN LEBAR MELALUI PENDEKATAN REPLACEMENT SERIES (RELATIVE COMPETITIVE ABILITY OF SUGARCANE EVENT-5 AND ITS PRIMARY WEEDS USING REPLACEMENT SERIES APPROACH) Netty Ermawati; Nurmala Sari; Novita Cholifah Ida; Bambang Sugiharto
Jurnal Ilmiah Inovasi Vol 16 No 1 (2016): April
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jii.v16i1.113

Abstract

Event-5 is a genetically modified (GM) sugarcane that engineered by introducing the Sucrose-Phosphate Synthase (SPS) gene to enhance the sugar productivity. To fulfill the security requirements of genetically modified products in the safety agriculture and the enviroment, it is necessary to test the competitive ability to the primary weeds which grow in the sugarcane cultivation areas. This study aimed to determine whether the transgenic sugarcane (Event-5) potentially invasive to the weeds at the critical stage of growth, that could effect the environmental balances. This research was conducted at Screen House and Laboratory for Biosciences, State Polytechnic of Jember, designed using the replacement series method in a Randomized Complete Block Design without factorial treatment. The combination treatments were Event-5, Non-transgenic sugarcane (NT) and the weeds (Ciperus rotundus and Portulaca olecarea) densities which consists of thirty-three treatments. The competitive ability test was determined by the rate of vegetative growth and the relative dry weight biomass of Event-5, NT plant and the weeds. The results indicated that the growth rate of Event-5, as well as the accumulation of dry weight of transgenic sugarcane were highly decrease than those of the weeds. The relative crowding coefficient (RCC) value of Event-5 showed lower compared to the P. olecarea weed, and sligtly higher to the C. rotundus weed. The RCC value of weed (C. rotundus) was higher than the RCC value of Event-5, suggested that the weed was potentially invasive to the transgenic sugarcane, and the Event-5 was not potential as a weed. 
OPTIMASI TRANSFORMASI GENETIKA MELALUI AGROBACTERIUM PADA TANAMAN PADI Muhammad Hazmi; Netty Ermawati; Bambang Sugiharto
AGRITROP Vol 12, No 1 (2014): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agr.v12i1.694

Abstract

Keberhasilan transformasi genetika pada tanaman padi sangat dipengaruhi oleh karakternya yang bersifat recalsitrant, terutama padi jenis indica yang banyak ditanam oleh petani di Indonesia. Oleh karena itu, optimasi metode selalu diperlukan sebelum melakukan transformasi genetika pada tanaman padi. Transformasi gen GUS sering digunakan sebagai sarana optimasi metode transformasi sebelum melakukan overekspresi gen yang sesungguhnya. Penelitian ini bertujuan untuk memastikan bahwa komponen transformasi gen melalui Agrobacterium tumefaciens dapat bekerja secara efektif pada tanaman padi. Penelitian dilaksanakan di Laboratorium Biosain Politeknik Jember dari bulan Maret sampai dengan Desember 2013. Konstruk gen GUS diperoleh dari Laboratorium Biologi Molekuler Universitas Jember. Padi yang digunakan adalah varietas Inpari 20 dari BPTP Malang dan CV Dongjin dari Korea Selatan sebanyak 20 biji dari setiap verietas. Hasil penelitian menunjukkan bahwa transformasi gen GUS melalui Agrobacterium tumefaciens berhasil menginfeksi eksplan. Eksplan yang diinfeksi tumbuh pada media seleksi antibiotik higromisin sampai umur 65 hari setelah infeksi. Hasil uji GUS menunjukkan bahwa gen GUS tidak terekspresi pada tanaman Padi. Hal ini menunjukkan bahwa ada kemungkinan DNA promoter dan plasmit pCAMBIA tidak kompatibel pada tanaman padi. Agrobacterium tumefaciens dapat digunakan untuk transformasi genetika pada tanaman padi, tetapi perlu DNA promoter dan plasmit yang kompatibel.Kata kunci: Optimasi, transformasi, gen GUS,  Agrobacterium tumefaciens, dan padi.
Co-Authors Bambang Sugiharto Muhammad Hazmi Novita Cholifah Ida Nurmala Sari