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S Murtini
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Immunomodulatory effect of tea mistletoe (Scurrula oortiana) extract on chicken embryos Murtini, S; Murwanti, R; Satrija, F; Hadnharyani, E
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.112 KB) | DOI: 10.14334/jitv.v11i3.525

Abstract

Tea mistletoe is one of medicinal herb which believed has an anticancer activity, it’s due to the capability of immunostimulator. The following research was carried out to determine the immunomodulatory effect of tea mistletoe (Scurrula oortiana) extract on chicken embryos. Twenty White Leghorn Specific Pathogen Free (SPF) 10 days old embryonated chicken eggs were divided into four groups of 5 eggs. The first group served as control and they were inoculated with aquabidestilate sterile. The second, third and fourth group was inoculated with 0.1 mg, 0.2 mg, and 0.4 mg S. oortiana extract/egg respectively. S. oortiana extract was inoculated via allantoic cavity. All experimental eggs were incubated at 37oC until day 21 and incubation was terminated before the embryos hatched. The embryos and the lymphoid organs (bursa of Fabricius, thymus and spleen) were weighed. Immunomodulatory effect of tea mistletoe extract was measured by counting the percentage of bursa of Fabricius active lymphoid follicle and the area of thymus medulla. The results showed tea mislestoe extract at the dose of 0.1mg, 0.2 mg and 0.4 mg have immunomodulatory effect on chicken embryos indicated by the increase of percentage of active lymphoid follicle of bursa Fabricius i.e. 68.8, 71.8 and 57.8% and increase area of thymus medulla i.e. 24.9 – 39.3% respectively compared to control group i.e. 22.6% of active lymphoid follicle of bursa Fabricius and 17.6% of thymus medulla area. It is concluded that S. oortiana extract at the dose of 0.1mg, 0.2 mg and 0.4 mg have immunomodulatory effect on chicken embryos. Key Words: Scurrula oortiana, Tea Mistletoe, Embryonated Chicken Egg, Immunomodulator
Prospek Pemanfaatan telur ayam berkhasiat anti virus avian influenza dalam usaha pengendalian infeksi virus flu burung dengan pendekatan pengebalan pasif Wibawan IWT; Rd Soejoedono; S Murtini; IGK Mahardika
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 3 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (639.376 KB)

Abstract

Production of polyclonal antibody against avian influenza type H5N1 and H5N2 was done in horse, cavia, and chicken using respective commercial avian influenza vaccine.keyword: egg yolk, passive immunization, specific lgY against AI virus H5N1Produksi antibodi poliklonal dalam serum kuda, serum ayam petelur, kuning telur dan pada serum marmut telah dapat dilakukan dengan menyuntikkan antigen virus H5N1 dan H5N2 yang dikemas dalam bentuk vaksin.Kata kunci: IgY spesifik terhadap virus AI H5N1, imunisasi pasif, kuning telur
Immunomodulatory effect of tea mistletoe (Scurrula oortiana) extract on chicken embryos S Murtini; R Murwanti; F Satrija; E Handharyani
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 3 (2006): SEPTEMBER 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.112 KB) | DOI: 10.14334/jitv.v11i3.525

Abstract

Tea mistletoe is one of medicinal herb which believed has an anticancer activity, it’s due to the capability of immunostimulator. The following research was carried out to determine the immunomodulatory effect of tea mistletoe (Scurrula oortiana) extract on chicken embryos. Twenty White Leghorn Specific Pathogen Free (SPF) 10 days old embryonated chicken eggs were divided into four groups of 5 eggs. The first group served as control and they were inoculated with aquabidestilate sterile. The second, third and fourth group was inoculated with 0.1 mg, 0.2 mg, and 0.4 mg S. oortiana extract/egg respectively. S. oortiana extract was inoculated via allantoic cavity. All experimental eggs were incubated at 37oC until day 21 and incubation was terminated before the embryos hatched. The embryos and the lymphoid organs (bursa of Fabricius, thymus and spleen) were weighed. Immunomodulatory effect of tea mistletoe extract was measured by counting the percentage of bursa of Fabricius active lymphoid follicle and the area of thymus medulla. The results showed tea mislestoe extract at the dose of 0.1mg, 0.2 mg and 0.4 mg have immunomodulatory effect on chicken embryos indicated by the increase of percentage of active lymphoid follicle of bursa Fabricius i.e. 68.8, 71.8 and 57.8% and increase area of thymus medulla i.e. 24.9 – 39.3% respectively compared to control group i.e. 22.6% of active lymphoid follicle of bursa Fabricius and 17.6% of thymus medulla area. It is concluded that S. oortiana extract at the dose of 0.1mg, 0.2 mg and 0.4 mg have immunomodulatory effect on chicken embryos.Key Words: Scurrula oortiana, Tea Mistletoe, Embryonated Chicken Egg, Immunomodulator
Co-Authors E Hadnharyani E Handharyani F Satrija I Gusti Ngurah Kade Mahardika R Murwanti Rd Soejoedono Wibawan IWT