The Biology learning process in the eight grade class of Junior High School IX Bandar Lampung is dominated by the teacher whose uses lecuring teaching strategis. This situation needs students to note the information fast and properly. The purpose of this study was to determine the effect of using mind map noting technique to improve students' biotechnology concepts mastery and students' learning activities. Students of class IXE and class IXC was chosen as sample using randomize block design sampling technique. The study used pre-post test non equivalent design, and obtained of the quantitative data using and N-gain score.The qualitative data were aspects of students` learning activities. Quantitative data were analyzed using SPSS 17 software while the qualitative data were analyzed descriptively.The results showed that the effect of mind map noting technique had not significantly improved the students`s concepts mastery. However the students` activities increased as much as 23.06%. Thus, the use of mind map noting techniques can influence students` learning activities and students` mastery of biotechnology concepts. Keywords: mind map, activity, concept, biotechnology.