Claim Missing Document
Check
Articles

Found 7 Documents
Search

MORPHOLOGY AND CELL BIOMASS OF SPONGE Aaptos aaptos AND Meutia Samira Ismet; Dedi Soedharma; Hefni Effendi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 3 No. 2 (2011): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (362.701 KB) | DOI: 10.29244/jitkt.v3i2.7829

Abstract

Aaptos aaptos and Petrosia sp. sponges are known for their ability to produce potential marine bioactive compound. As a metazoan animal with simple body structure, the morphology and it association with symbiont-bacteria could influence their bioactive compound both type and activity, as much as their habitat adaptation. In order to determine morphology and its cell biomass of Aaptos aaptos dan Petrosia sp., samples were taken from the West Pari Island, at 7 m depth. Preserved samples (in 4% formaldehyde) were examined using a histological mounting and centrifugation method to separate the cells fraction of sponge’s tissues. A. aaptos sponge has a soft body structure with 55.9% skeleton-forming fraction, 14.2% sponge cell fraction and 29.9% bacteria fraction. Meanwhile, Petrosia sp. sponge has a rigid body with dominant skeleton-forming fraction (68.6%), and lesser sponge cell and bacteria associated (19.7% and 11.7%, respectively).Keywords: A. aaptos, Petrosia sp, morphology, cell biomass
COMPOSITION AND ANTIBACTERIAL ACTIVITIES OF MARINE SPONGES FROM DIFFERENT SEAGRASS ECOSYSTEMS IN KEPULAUAN SERIBU WATERS, JAKARTA Meutia Samira Ismet; Dietriech G. Bengen; Ocky Karna Radjasa; Mujizat Kawaroe
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (779.723 KB) | DOI: 10.29244/jitkt.v8i2.15838

Abstract

A seagrass ecosystem has high productivity that supports many species of associated invertebrates, including sponges. However, seagrass beds with different habitat condition, including seagrass species composition and architectural morphotypes could affect the associative sponge in the ecosystem.   This study is aimed to find out about sponge species composition in different seagrass beds. The observation was conducted in two seagrass ecosystems at the east (site 1) and at the southeast (site 2) of Pramuka Island, Kepulauan Seribu, near Jakarta Bay, Indonesia. The Belt Transect was used to assess the density of sponges on the seagrass ecosystem, which was placed parallel to the coastline. The sponges species has higher number at the east than the southeast, with both sites was dominated by Spirastrella sp. and Agelas conifera. Correspondence Analysis (CA) results showed that sponges community has close interaction with seagrass abundance especially Cymodocea rotundata and Enhalus acoroides and architectural characteristic (patchily or continues meadows). Antibacterial assay of sponges tissue showed that only 7 sponge species has activity against targeting bacteria. The CA results also showed that sponge antibacterial activity was not correlated with seagrass species, with low bactericide and bacteriastatic activities. The implication of this result showed that sponges community can grow up at seagrass ecosystem eventhough their potential secondary metabolite activities is very low probably due to lack of stimulus mechanisms in the environment. 
AKUMULASI MIKROPLASTIK PADA SPESIES IKAN EKONOMIS PENTING DI PERAIRAN PULAU BARRANGLOMPO, MAKASSAR Rahmat Sawalman; Neviaty Putri Zamani; Shinta Werorilangi; Meutia Samira Ismet
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 13 No. 2 (2021): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v13i2.34587

Abstract

Microplastics (MPs) have been found in various marine compartments especially in fish, and there is limited information regarding the accumulation of MPs in fish organs. Therefore, this study aimed to investigate the presence of microplastics in economically important fish organs (Hemiramphus far, Siganus virgatus, and Lethrinus lentjan) at Barranglompo Island Waters, Makassar, and also to identify the characteristics of microplastics including color, shape, and size. Each organ was extracted using KOH 20% and the MPs characteristics were observed visually using a stereomicroscope. The result showed that MPs were found in gills, gastrointestinal tract, and flesh of three fish species. The detection rate of MPs in H. far, S. virgatus, and L. lentjan was 100%, 100%, and 82%, respectively. The most common MP color was blue, the shape was predominantly line, and the most abundant size range was 1–5 mm. Further studies are needed to investigate the accumulation of MPs in fish organs experimentally in the laboratory.
STRUKTUR DAN SEBARAN VEGETASI MANGROVE BERDASARKAN KUALITAS LINGKUNGAN BIOFISIK DI TAMAN HUTAN RAYA NGURAH RAI BALI Ajie Imamsyah; Dietriech Geoffrey Bengen; Meutia Samira Ismet
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 14 No 1 (2020)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.577 KB) | DOI: 10.24843/EJES.2020.v14.i01.p08

Abstract

Mangrove are typical coastal plants whose habitat is influenced by tidal and substrate characteristics. Mangrove ecosystems have a variety of functions that can be utilized both in terms of physical, biological, and economic. Utilization of mangrove ecosystems that do not pay attention to environmental sustainability and conservation aspects cause damage of a whole coastal ecosystems. This study aims at analyze the structure and distribution of mangrove vegetation based on biophysical environment quality. This research was conducted on February until March 2017 at Ngurah Rai Forest Park Bali. The results of study show that the conditions of temperature, pH, and salinity are still in accordance with the specified quality standards, however dissolved oxygen has been classified as polluted by anthropogenic waste. The analysis of the sturcture and distribution of mangrove vegetation found that species of S. alba dominated at the location research, followed by oleh R. apiculata, R. mucronata, B. gymnorrhiza, R. stylosa, X. granatum, A. marina, and A. corniculatum. Overall, the results showed that the mangrove condition was in the damaged cateogry with a mangrove density value < 1000 ind/ha. Keywords: Coastal, Environmental, Mangrove, Vegetation
Kontaminasi Mikroplastik pada Perna viridis di Teluk Lampung Zakiyah Rahim; Neviaty Putri Zamani; Meutia Samira Ismet
Jurnal Kelautan Tropis Vol 25, No 1 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i1.12722

Abstract

Fisheries activity in Lampung Bay which has been very active in recent years is the cultivation of green mussels (P. viridis) for consumption. Located in Teluk Betung Timur sub-district, this is considered appropriate because of the environmental parameters that support the life of P. viridis. The location of P. viridis cultivation is not far from coastal settlements (±500-600 m from settlements). Anthropogenic activities of coastal residents as well as the use of plastic materials in the cultivation are concerned to be the source of microplastic contamination that found in P. viridis. This study aims to determine the presence and characteristics of microplastics in P. viridis as well as to compare the abundance of microplastics in the gills and digestive organs. Taking 30 P. viridis by purposive sampling with homogeneous size. The samples were analyzed by microplastics in chemical oceanography and biology micro Laboratory, FPIK, IPB University. P. viridis tissue was destructed by using 10% KOH solution at a ratio of 1:3 (m/v) at 60 °C for 24 hours and then filtered using a 1.6 µm Whatman GF/A filter paper. Abundance of microplastics in whole soft tissue were 0.53±0.7 mgl/g. The gill organs were found to be more microplastic than the digestive organs (p<0.05). The fragments microplastic is most commonly found in intact tissues and digestive organs, while fiber is mostly found in the gills of P. viridis. Blue color and size <100 µm were most commonly found in intact tissues, gills and digestive organs of P. viridis.  Kegiatan perikanan di Teluk Lampung yang sangat aktif dalam beberapa tahun ini yaitu kultivasi kerang hijau (P. viridis) konsumsi. Berlokasi di kecamatan Teluk Betung Timur, hal ini dinilai tepat karena parameter lingkungan yang menunjang kehidupan P. viridis. Lokasi pembesaran P. viridis tidak jauh dari pemukiman penduduk pesisir (±500-600 m dari pemukiman). Kegiatan antropogenik penduduk pesisir juga penggunaan material plastik pada sistem pembesaran dikhawatirkan dapat menjadi sumber cemaran mikroplastik yang terdapat pada P. viridis. Penelitian ini bertujuan untuk mengetahui keberadaan dan karakteristik mikroplastik pada P. viridis juga membandingkan kelimpahan mikroplastik pada insang dan organ pencernaan. Pengambilan 30 P. viridis secara purposive sampling dengan ukuran homogen. Analisis mikroplastik dilakukan di laboratorium oseanografi kimia dan biologi mikro, FPIK, Universitas Pertanian Bogor. Destruksi jaringan P. viridis dengan menggunakan larutan KOH 10% perbandingan 1:3 (m/v) pada suhu 60 °C selama 24 jam lalu difiltrasi menggunakan filter paper whatman GF/A 1,6 µm. Mikroplastik tubuh kerang utuh yaitu 0,53±0,7 mgl/g. Organ insang lebih banyak ditemukan mikroplastik dibandingkan organ pencernaan (p<0,05). Bentuk fragmen paling banyak ditemukan di jaringan utuh dan organ pencernaan, sedangkan fiber banyak ditemukan di insang P. viridis. Warna biru dan ukuran <100 µm paling banyak ditemukan di jaringan utuh, insang maupun organ pencernaan P. viridis.
The Effect of Plastic Waste Attachment on Branching Coral to Zooxanthellae Abundance in the Kelapa Dua Island Waters, Seribu Islands Fadel Muhammad; Neviaty Putri Zamani; Meutia Samira Ismet; Muhammad Irlan Assidiq Kusuma Ramadhan
Jurnal Ilmiah Perikanan dan Kelautan Vol. 13 No. 1 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v13i1.18146

Abstract

HighlightLight intensity from plastic waste was positively correlated with the abundance of zooxanthellae in corals.The abundance of zooxanthellae between sack plastic and clear plastic treatment tended to be the same.The treatment of packaging plastic was treated with the lowest light intensity.The treatment of black plastic was the treatment that produced the lowest abundance of zooxanthellae. AbstractCoral growth in Indonesia was being disrupted due to the continued threat of environmental change, such as littering to the beach. This study aimed to analyze the effect of the attachment of different colored plastic waste to the rate of zooxanthellae abundance on branching corals in Kelapa Dua Island waters. Branching coral colony samples consisted of the genus Acropora and Porites with a total of 15 fragments of coral colonies at the depth of 80-90 cm were tested with a treatment of the attachment plastic wastes (control, clear plastic, plastic sack, plastic packaging, and black plastic) for four days. The zooxanthellae abundance was calculated using the APHA (American Public Health Association) formula. The results of zooxanthellae abundance on the closure treatment of branching coral samples with black plastic and control had the lowest and highest values by 4.25 x 105 cells/cm2 and 1.38 x 106 cells/cm2. The results of the linear regression test showed that there was a significant relationship between plastic waste with low light intensity and a decrease of zooxanthellae abundance in coral colonies with the equation Y of 972.78x + 653402, determination index (R2) of 0.68, and correlation index (r) of 0.83. These results indicated that the attachment of plastic waste to branching coral colonies could be a stressor on coral animals in reducing light intensity, which caused a reduction of zooxanthellae abundance as coral endosymbiont.
Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Siti Maysaroh; Meutia Samira Ismet; Beginer Subhan; Rita Andini; Enny Rimita Sembiring; Nurlita Putri Anggraini
Depik Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column