Dida Akhmad Gurnida
Department of Child Health, Faculty of Medicine, Unversitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung

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Association of Chest X-Rays Features with the Length of Stay in Suspected COVID-19 Status Zulmansyah Zulmansyah; Gaga Irawan Nugraha; Dwi Agustian; Dida Akhmad Gurnida
Global Medical & Health Communication (GMHC) Vol 9, No 2 (2021)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.315 KB) | DOI: 10.29313/gmhc.v9i2.7905

Abstract

Coronavirus disease 2019 (COVID-19) is a new type of respiratory infection that first emerged in December 2019 in Wuhan, Hubei, China. COVID-19 is caused by a new variant of virus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Examining the chest x-rays is one technique to diagnose COVID-19. This study aims to determine the association of chest x-rays features in suspected COVID-19 patients with the length of stay at Al-Ihsan Regional General Hospital West Java Province and Banten Regional General Hospital in June–November 2020. The subject was 29 COVID-19 people were confirmed, whereas 31 people were not. The statistical analysis used Pearson correlation and multi-linear regression. This study found that tuberculosis had a strong association with length of stay (p value=0.048, association=0.4), and also there is a robust association between bilateral pneumonia and duration of stay (p value=0.028, association=0.873). A linear regression model discovery of TB chest x-rays on the subject raises the treatment by 0.5867 days. In addition, the discovery of bilateral pneumonia increases the length of stay of treatment by 0.32218 days more than the discovery of unilateral pneumonia. In general, tuberculosis, bacterial pneumonia, viral pneumonia, and bilateral pneumonia together affected the outcome of length of stay of patients with suspected COVID-19. ASOSIASI GAMBARAN RONTGEN TORAKS DENGAN LAMA RAWAT PADA PASIEN SUSPEK COVID-19Coronavirus disease 2019 (COVID-19) adalah jenis infeksi saluran pernapasan baru yang pertama kali mewabah pada Desember 2019 di Wuhan, Hubei, Cina. COVID-19 disebabkan oleh varian virus baru yang disebut severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Pemeriksaan rontgen toraks merupakan salah satu teknik mendiagnosis COVID-19. Penelitian ini bertujuan mengetahui asosiasi gambaran rontgent toraks pada pasien suspek COVID-19 dengan lama rawat inap di RSUD Al-Ihsan Provinsi Jawa Barat dan RSUD Banten pada Juni–November 2020. Subjek penelitian adalah 29 orang terkonfirmasi COVID-19 dan 31 orang tidak. Analisis statistik menggunakan korelasi Pearson dan regresi multilinier. Penelitian ini menemukan bahwa tuberkulosis memiliki hubungan yang kuat dengan lama perawatan (p=0,048; asosiasi=0,4) dan juga terdapat hubungan yang kuat antara pneumonia bilateral dan lama perawatan (p=0,028; asosiasi=0,873). Penemuan model regresi linier rontgent toraks TB pada subjek meningkatkan risiko perawatan 0,5867 hari lebih lama. Selain itu, penemuan pneumonia bilateral meningkatkan risiko perawatan 0,32218 hari lebih lama dibanding dengan penemuan pneumonia unilateral. Secara umum, tuberkulosis, pneumonia bakteri, pneumonia viral, dan pneumonia bilateral secara bersama-sama berpengaruh terhadap luaran lama rawat inap pasien suspek COVID-19.
Effect of ESAT-6 on Phagocytosis Activity, ROS, NO, IFN-γ, and IL-10 in Peripheral Blood Mononuclear Cells of Pulmonary Tuberculosis Patients Dicky Santosa; Dida Akhmad Gurnida; Herri S. Sastramihardja; Anas Subarnas
Global Medical & Health Communication (GMHC) Vol 10, No 2 (2022)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.913 KB) | DOI: 10.29313/gmhc.v10i2.9797

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) that lives intracellularly. MTB can inhibit lysosomal enzymes and phagolysosomal fusion, which is challenging to eliminate. These are due to ESAT-6/CFP-10 originating from the RD1 region genome that expresses the Esx-1 type VII secretion system. Esx-1 encodes Esx-A (ESAT-6) and Esx-B (CFP-10), potential vaccine candidates still under research and development. ESAT-6/CFP-10 is predicted to affect macrophage phagocytic activity, IFN-γ, ROS/NO, and IL-10 levels. Several studies have begun to focus on the ESAT-6 antigen due to the high levels of ESAT-6 antibody found in pleural effusion and granuloma fluid. They can last up to 1 year compared to CFP-10 in experimental animals. This study aimed to analyze the effect of ESAT-6 on the phagocytic activity of macrophages, ROS/NO, IFN-γ, and IL-10 in peripheral blood mononuclear cells (PBMCs) cultures of pulmonary TB patients. It is experimental laboratory research with a post-test-only control group design with PBMCs from October 2019 to December 2020 in the Aretha Laboratory Bandung. There were two groups: the negative group (without ESAT-6) and the positive group (with ESAT-6). Six subjects were sampled at the Pindad Hospital in Bandung, and the research was carried out at the Aretha Laboratory in Bandung. Statistical analysis using paired t test. There was a significant difference between the negative and positive groups (p<0.05). ESAT-6 can decrease macrophage phagocytic activity, intracellular ROS/NO, and IFN-γ but increase IL-10 levels.