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All Journal Indonesian Journal of Applied Sciences Majalah Kedokteran Bandung Althea Medical Journal Jurnal Farmasi Klinik Indonesia Global Medical and Health Communication UNEJ e-Proceeding The Indonesian Biomedical Journal Sari Pediatri Jurnal Sistem Kesehatan Bunda Edu-Midwifery Journal (BEMJ) Majalah Kedokteran Neurosains Jurnal Integrasi Kesehatan dan Sains Jurnal Riset Kedokteran Bandung Conference Series : Medical Science
Herri S. Sastramihardja
Bagian Farmakologi, Fakultas Kedokteran, Universitas Islam Bandung, Bandung
Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Soursop Effect in Cervical Cancer Apoptosys Mechanism Yuniarti, Lelly; Sastramihardja, Herri; Purbaningsih, Wida; Tejasari, Maya; Respati, Titik; Hestu, Enggar; Adithya, Agly
Global Medical & Health Communication (GMHC) Vol 2, No 1 (2014)
Publisher : Fakultas Kedokteran Universitas Islam Bandung

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Abstract Cervical cancer is the fifth leading cancer cause of women death  in Indonesia.  Acetogenin, flavonoids, and tannins in sour sop leaves have anti-cancer effects through regulated genes which involved in apoptotic process such as in Caspase-3. This study aimed to determine the effect of ethanol extract of sour sop leaves to apoptosis and Caspase-3 gene expression in HeLa cell cultures. This was an in vitro study using HeLa cell culture samples  divided into 4 groups. The first group was HeLa cell cultures without  sour sop leaves ethanol extract. The 2nd ,3rd, and 4th group were HeLa cells cultures which were given sour sop leaves ethanol extract with concentration of 60 μg/mL, 120 mg / mL, and 240 mg / mL respectivelly. Apoptosis in each group was examined using TUNEL method and the expression of the caspase 3 gene by RT-PCR. One Way ANOVA with confidence level of 95% were used as statistical analysis.The result showed the effect of the sour sop leaves ethanol extract increased the apoptosis percentage in HeLa cells culture but did not affect the gene expression of Caspase-3.Key words: apoptosis, caspase-3, sour sop leaves Efek Daun sirsak dalam Mekanisme Apoptosis Kanker Serviks Abstrak Kanker serviks adalah penyebab kematian kelima untuk wanita di Indonesia. Asetogenin, flavonoid, dan tannin dalam daun sirsak terbukti mempunyai pengaruh anti kanker melalui regulasi gen melalui proses apoptosis seperti pada caspase-3. Penelitian ini bertujuan untuk menentukan efek ekstrak etanol daun sirsak untuk apoptosis dan ekspresi gen caspase-3 dalam kultur sel HeLa. Penelitian ini adalah penelitian in vitro menggunakan sel kultur HeLa yang dibagi menjadi 4 kelompok. Grup pertama adalah sel kultur HeLa tanpa ekstrak etanol daun sirsak sedangkan grup dua, tiga dan empat adalah sel kultur HeLa yang mendapat ekstraks etanol daun sirsak dengan konsentrasi  60μg/mL, 120 mg / mL, dan 240 mg / mL secara bertururt turut. Apoptosis dalam setiap kelompok diperiksa dengan menggunakan metode  terminal deoxynucleotidyl transferase-mediated (TUNEL) dan ekspresi gen caspase-3 dengan menggunakan reverse transciptase-polimerase chain reaction (RT-PCR). One Way analysis of varians (ANOVA) dengan tingkat kepercayaan 95% digunakan untuk analisis statistik. Hasil penelitian menunjukkan bahwa ekstrak etanol daun sirsak meningkatkan persentase apoptosis dari kultur sel HeLa tetepi tidak berpengaruh pada ekspresi gen Caspase- 3. Kata kunci: Apoptosis, Caspase-3, ekstrak daun sirsak 
Peran Kedelai (Glycine Max L) dalam Pencegahan Apoptosis pada Cedera Jaringan Hati Tejasari, Maya; Shahib, Nurhalim; Iwan, Djanuarsih; Sastramihardja, Herri S
Global Medical & Health Communication (GMHC) Vol 2, No 1 (2014)
Publisher : Fakultas Kedokteran Universitas Islam Bandung

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Abstrak Pada liver injury akibat berbagai sebab, terjadi apoptosis sel yang sangat banyak yang dapat memengaruhi fungsi metabolik hati.  Isoflavon kedelai telah diketahui dapat mencegah apoptosis sel pada folikel ovarium dan osteoblas. Penelitian ini bertujuan untuk mengetahui pengaruh kedelai pada pencegahan apoptosis sel pada jaringan hati mencit yang diinduksi CCl4.  Penelitian dilakukan menggunakan 30 ekor mencit jantan galur DDY berumur 8─10 minggu yang dibagi dalam 6 kelompok perlakuan.  Kelompok 1 merupakan kontrol positif yang hanya diberi makanan pelet standar selama 3 minggu kemudian diberi 0,2 mL larutan CCl4 per oral selama 4 hari. Kelompok 2 merupakan kontrol negatif yang hanya diberi makanan pelet standar dan tidak diberi CCl4, sedangkan kelompok 3─6 merupakan kelompok uji yang selain diberi makanan pelet standar juga diberi kedelai dengan kadar berturut-turut 145,6 mg/hari, 218,4 mg/hari, 291,2 mg/hari dan 364 mg/hari selama 3 minggu kemudian diberi 0,2 mL larutan CCl4 peroral selama 4 hari.  Seluruh kelompok kemudian dikorbankan dan diambil organ hatinya untuk dilakukan pemeriksaan histokimia terminal deoxynucleotidyl transferase-mediated dUTP Nick end labeling (TUNEL).  Parameter yang diukur adalah jumlah apoptosis sel pada sayatan jaringan hati mencit menggunakan mikroskop cahaya.  Data disajikan dan dianalisis secara statistik menggunakan uji analysis of varians (ANOVA) untuk menganalisis perbedaan antar kelompok. Hasil penelitian memperlihatkan bahwa dari hasil pemeriksaan imunohistokimia TUNEL tampak jumlah sel yang mengalami apoptosis pada kelompok yang diberi kedelai lebih sedikit dibandingkan dengan kelompok yang tidak diberi kedelai. Analisis uji ANOVA antara kelompok tersebut menunjukan perbedaaan yang signifikan dengan nilai p<0,05. Simpulan, bahwa pemberian kedelai dapat mencegah apoptosis sel pada jaringan hati mencit yang diinduksi CCl4.  Kata kunci : Apoptosis,  CCl4, isoflavon, kedelai, liver injury, TUNEL Soy (Glycine max L.) Prevent Apoptotic Cells in Liver Tissue Injury Abstract In the state of liver injury by any cause there are numerous apoptotic cells influencing metabolic function of the liver.  Soy isoflavone (Glycine max L.) known to have effect that inhibit apoptotic cells in follicle and osteoblast.  The aim of this study is to evaluate whether soy has anti apoptotic effect of  CCl4 induced liver  injury in mice. This study use 30 male DDY mice 8─10 weeks old, divided into 6 groups.   Group I acted as positive control, received standard pellet for 3 weeks and induced by 0,2 mLCCl4 per oral.  Group II, the negative control, received only standard pellet.  Group III─VI received standard pellet and treated by soybean extract 145.6 mg, 218.4 mg, 291.2 mg and 364 mg per day respectively administrated orally for 3 weeks and then induced by 0.2 ml CCl4 per oral.  After 4 days of CCl4 induced, the effect of soybean extract was evaluated using histo-chemistry evaluation Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).  The identification and quantification of the apoptotic cells in mice liver tissue were done using light microscopy and showed that the TUNEL immune-histochemical examination. The results showed that the number of cells undergoing apoptosis in the group treated by soybean extract were less than the group that was not treated. The results enhanced by analysis of varians (ANOVA)  between the groups showed a significant difference with p<0.05. In conclusion, soy administrated orally could  prevent apoptotic cells in liver tissue.   Key words:  Apoptotic, CCl4, isoflavone, liver injury, soybean, TUNEL 
Solanumnigrum L. as a Hepatoprotective Agent Rizky, Muthiana; Sastramihardja, Herri S.; Nur, Ismet Muchtar
Althea Medical Journal Vol 2, No 1 (2015)
Publisher : Althea Medical Journal

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Abstract

  Background: Liver damage may be caused by various factors. SolanumnigrumL.fruit is known to contain flavonoid antioxidant which is responsible for its hepatoprotective effect. A study was conducted to determine the protective effect of Solanumnigrum L. fruit infusion (SNFI) on CCl4-induced hepatic cell damage in rats. Methods: A complete randomized experimental study was conducted on 25 male Wistar strain-white rats (Rattusnorvegicus) which were divided into five groups during the period of September– October 2012. Group I (negative control) was given standardized food and water; group II (positive control) was induced by carbon tetrachloride (CCl4) 10% paraffin intraperitoneally by 8 mL/kg body weight on the 8th day of the study; group III, IV, V (treated) were given Solanumnigrum L. fruit infusion (SNFI) by 22.5g/100mL, 45 g/100mL and 90g/100mL concentrations for 8 days, respectively, prior to CCl4 induction. The calculation of necrotic liver cells was performed in 48 hours after induction. Data were statistically analyzed using Kruskal-Wallis test followed by Mann-Whitney post-hoc test. Results: The percentage of necrosis liver cells in group III, IV, and V was smaller compared to  the positive control group. The protective effect of SNFI against CCl4-induced hepatotoxicity may be related to its ability to elevate the antioxidant agent in the body. There were significant differences in necrotic between Group II and group III, IV, V which were treated with SNFI. Conclusion: Further investigation is required to characterize the active ingredients and the mechanism of SNF action to confirm the hepatoprotective and antioxidant effects.Keywords: CCl4, hepatoprotective, Solanumnigrum L.DOI: 10.15850/amj.v2n1.433  
The Effect of Breadfruit Leaves Infusion on Acute Renal Failure Rat model Abstract Hardani, Muhamad Risdan; Sastramihardja, Herri S.; Afiatin, Afiatin
Althea Medical Journal Vol 2, No 4 (2015)
Publisher : Althea Medical Journal

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Background : Empirically, breadfruit (Artocarpus altilis) leaf has been used as traditional medicine for several diseases, such as acute renal failure. This research is aimed at finding the effects of breadfruit leaf infusion on blood creatinine levels in rats to model acute renal failure.Methods : This research is an experimental study, using 30 male wistar rats, weighing 150–250 g, divided into 5 groups. The first group is a negative control and the second group is a positive control. Groups 2–5 were induced with Gentamicine and peroxicam for 7 days, and then groups 3–5 given infusion of breadfruit leaf in different doses for 7 days, and then blood creatinine levels were tested at the 15th day. This data was analyzed using a multiple comparison Dunnett T3 test because this data is not homogenous. This research was done in pharmacology laboratorium universitas padjadjaran from Oktober–November 2012.Result : Bases on average creatinine levels of 0.62, 0.87, 0.98, and 0.87 mg/dL observed for the diffetent groups, it was statistically show that significantly different creatinine levels, differing by 0.038 (p < 0.05) were observed for the control group and the group which was given an infusion of breadfruit leaf.Conclusion : It can be concluded that infusions of breadfruit leaf do have an effects on blood creatinine levels in acute renal failure.Key words : acute renal failure, breadfruit leaf, creatinine  DOI: 10.15850/amj.v2n4.527
Knowledge and Attitude towards Prevention and Management of Hypertension in Jatinangor Sub-district Malahayati, Kartika; Supriyadi, Rudi; Sastramihardja, Herri S.
Althea Medical Journal Vol 3, No 2 (2016)
Publisher : Althea Medical Journal

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Background: There is an increase in prevalence of hypertension all over the world as well as Indonesia. Hypertension affects almost all body organs and systems. Hypertension prevention and management are influenced by knowledge and attitude towards the disease. The aim of this study was to assess knowledge and attitude towards prevention and management of hypertension.Methods: This was a descriptive study with cross–sectional approach conducted in Jatinangor sub-district in 2013. Sample sizes were determined based on proportion of the case in population and precision estimates (95% confidence intervals). One hundred twenty respondents aged 18–60 year-old were selected from population using stratified random sampling method. Hypertensions knowledge and attitude were assessed using validated questioner as the scale measurement. Frequency of knowledge level was presented as three ordinal categories; good, moderate, and poor. Attitude of hypertension was presented as frequency in two categories; relatively positive and relatively negative.Results: Out of 120 respondents administered the questioner, 42.50% had good knowledge; 41.67% had moderate knowledge; 15.83% had poor knowledge related to hypertension prevention and 21.67% had good knowledge; 19.17% had moderate knowledge; 59.17% had poor knowledge related to management of hypertension. Hypertension attitude of respondents were 60.83% relatively positive in topics of hypertension prevention and 39.17% in topics of hypertension management.Conclusions: Respondents are knowledgeable about hypertension prevention but are less knowledgeable about its management. Respondents have relatively positive attitudes towards hypertension prevention. In the other hand, respondents have more relatively negative attitudes towards its management. [AMJ.2016;3(2):222–9]DOI: 10.15850/amj.v3n2.801
Polypharmacy Treatment of Hypertensionin Public Health Centers A/P Nayagam, Vasanthi; Afiatin, Afiatin; Sastramihardja, Herri S.
Althea Medical Journal Vol 3, No 4 (2016)
Publisher : Althea Medical Journal

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Abstract

Background: Hypertension is one of the most prominent global diseases. Despite the availability of effective therapies, hypertension remains poorly controlled in Indonesia. In many cases, patient’s noncompliance may be attributable to the low patients’ knowledge, attitude, and life-style practices such as polypharmacy. Polypharmacy is defined as the administration of many drugs at in one prescription. Polypharmacy increases expenses, possible adverse reaction to a single agent, incidence of drug interactions, and decreases patient’s compliance This study aimed to identify the practice of polypharmacy in hypertension treatment in primary health centers.Methods: A cross-sectional descriptive observational study was carried out on 60 patients from two primary health centers in Jatinangor, West Java, Indonesia in October 2013. Sociodemographic profile, degree of hypertension, types of antihypertensive drugs, concomitant drugs given together with antihypertensive drugs, and treatment compliance data were collected and presented in tables and figures.Results: The incidence of hypertension was more common among male patients compared to female patients. Thirty-three patients (55%) have low compliance to their medication. Twenty-nine patients (48%) received single drug and 31 patients (52%) received more than one drugs.Conclusions: The percentage of polypharmacy practice in treating hypertension in primary health centers is 52%. The most frequently prescribed anti-hypertensive are angiotensin-converting enzyme (ACE) inhibitors and calcium-channel blockers (CCB). Most of hypertensive patients have low compliance to therapy. [AMJ.2016;3(4):633–9] DOI: 10.15850/amj.v3n4.951
Hepatoprotective Effect of Solanum melongena/Eggplant against Acute Hepatitis Komara, Nabhan; Sastramihardja, Herri S.; Afiati, Afiati
Althea Medical Journal Vol 2, No 1 (2015)
Publisher : Althea Medical Journal

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Abstract

   Background: Hepatitis is a liver inflammation that can be acute or chronic and may cause damage to hepatocytes such as necrosis.. Hepatocyte necrosis can be inhibited by antioxidants such as flavonoids found in Solanum melongena fruit. This study aimed to determine the hepatoprotective effect of Solanum melongena fruit infusion to inhibit hepatocytes damage in CCl4-induced rats.Methods: Twenty five male Wistar rats were randomly divided into five groups, and adapted for 7 days before the experimental study. Negative and positive groups were given aquadest, Group III−V were given Solanum melongena fruit infusion containing 1.125 grams, 2.25 grams and 4.5 grams orally for 9 days. At the 9th day, all rats were induced by 8 mL/kgBW of 10% CCl4 in paraffin, except for the negative group. Rats were sacrificed on the 11th day, and liver biopsy preparations were made. Hepatocyte necrosis was counted and was analyzed by Kruskal-Wallis test and Mann-Whitney test. Results: The study showed that the percentage of necrotic hepatocytes in group III, IV a nd V were lower than in the positive group. Using Mann-Whitney test, there were significant differences in negative group, group III, and group V (p<0.05). Meanwhile, unsignificant difference was seen between the positive group and group IV (p>0.05). Kruskal-Wallis test showed that there weresignificantly differences among groups (p<0.05). Conclusions: Solanum melongena fruit infusion has hepatoprotective effects against acute hepatitis in rat model histopathologically.Keywords: CCl4, flavonoids, hepatocyte, necrosis, Solanum melongena DOI: 10.15850/amj.v2n1.434  
Analgesic Usage in Elderly at Public Health Center: A study in West Java, Indonesias Putro, Gembong Soeyono; Sastramihardja, Herri S.; Dwipa, Lazuardhi
Althea Medical Journal Vol 4, No 1 (2017)
Publisher : Althea Medical Journal

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Abstract

Background: Various analgesics prescriptions for elderly are not appropriate according to the guideline and can cause the increase of side effects such as gastric problems. Puskesmas as a public health center in Indonesia has an important role in anticipating this problem. The objectives of this study was to identify the  analgesic usage in elderly patients at the public health center. Methods: This retrospective descriptive study was conducted for 3 months at Tanjungsari public health center, Sumedang, West Java, Indonesia, using total sampling. The data was taken from 417 medical records from 2013. The data taken from medical records were: sex, analgesic drug, diagnosis, and drug for gastric problem.Results: From the collected data, the most analgesics prescribed for the elderly patients was paracetamol, followed by Piroxicam, Mefenamic acid, and Ibuprofen. Not all of the elderly patients who received NSAIDs, were given gastric drug. Conclusions: The most prescribed analgesic drug given to elderly patients at the public health center is paracetamol. [AMJ.2017;4(1):16–9]DOI: 10.15850/amj.v4n1.1014
Hand hygiene, health care workers, methicillin-resistant Staphylococcus aureus, nosocomial infection, Staphylococcus aureus Gunadi, Julius; Sastramihardja, Herri S.; Dewi, Nina Susana
Althea Medical Journal Vol 2, No 2 (2015)
Publisher : Althea Medical Journal

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Background: Hypercholesterolemia is a major risk factor for coronary heart disease. Ear fungus (Auricularia auricula) contains soluble fiber (beta glucan) which is believed to have an effect of reducing cholesterol level. The aim of this study was to determine the effect of fungus infusion in reducing blood cholesterol.Methods: This study used the laboratory experimental method conducted in the pharmacology laboratory of Dr. Hasan Sadikin General Hospital Bandung. The subjects were 25 male Wistar rats which were randomly divided into 5 groups. The first group was given only distilled water as a negative control, the remaining groups were induced to be dyslipidemic and received fungus infusion with different doses of 18%, 36%, 72% and one group received no treatment.Results: The comparison of different doses showed all had the effect of reducing cholesterol levels compared to the positive control group (p<0.05). Each dose showed slight differences in their effectiveness, 18% (p=0.007; p<0.05), 36% (p=0.002; p<0.05), and 72% (p=0.014; p<0.05).Conclusions: ear fungus infusion had the effect of reducing cholesterol with the most effective concentration was 36%. [AMJ.2015;2(1):153–61]
Evaluasi Kualitas Hidup dengan Kuesioner EQ-5D pada Pasien Kanker Serviks Rawat Inap Sebelum dan Setelah Kemoterapi Suwendar, Suwendar; Fudholi, Achmad; Andayani, Tri Murti; Sastramihardja, Herri S
Indonesian Journal of Clinical Pharmacy Vol 6, No 1 (2017)
Publisher : Indonesian Journal of Clinical Pharmacy

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Abstract

Kualitas hidup merupakan salah satu acuan keberhasilan dari upaya pengobatan termasuk kemoterapi pada pasien kanker. Pasien kanker yang menjalani kemoterapi dapat mengalami perubahan pada kualitas hidupnya. Tujuan penelitian ini adalah untuk menilai kualitas hidup pasien kanker serviks dengan menggunakan kuesioner EQ-5D sebelum dan setelah mendapatkan kemoterapi. Penelitian ini merupakan penelitian non-eksperimental bersifat analitik menurut perspektif pasien dengan teknik pengumpulan data secara prospektif. Subjek penelitian adalah pasien kanker serviks rawat inap kelas 3 yang memenuhi kriteria inklusi. Penelitian dilakukan di RSUP Dr. Hasan Sadikin Bandung. Data yang digunakan adalah data pasien dari bulan Juni sampai Desember 2015. Penelitian dilakukan dengan menggunakan kuesioner EQ-5D. Selanjutnya dilakukan perhitungan persentase permasalahan pasien, nilai indeks EQ-5D (utility) dan nilai EQ-5D VAS. Nilai utility dan EQ-5D VAS dianalisis secara statistik dengan uji Wilcoxon (α=5%) untuk melihat terjadinya perubahan kondisi yang bermakna sebelum dan setelah kemoterapi. Hasil menunjukkan bahwa setelah kemoterapi siklus pertama, persentase masalah kemampuan berjalan/bergerak dan kegiatan yang biasa dilakukan mengalami peningkatan, rasa sakit/ tidak nyaman dan rasa cemas/depresi mengalami penurunan, sedangkan perawatan diri tidak mengalami perubahan. Berdasarkan hasil uji statistik, setelah kemoterapi siklus pertama, nilai utility dan EQ-5D VAS mengalami peningkatan dan menunjukkan peningkatan bermakna (nilai T hitung masing-masing 2,0 dan 4,5) pada pasien stadium I.  
Co-Authors A/P Nayagam, Vasanthi Achmad Fudholi Achmad Fudholi Achmad Fudholi Achmad Fudholi Adjat Sedjati Rasyad Afiati Afiati Afiati Afiati Afiatin Afiatin Afiatin, Afiatin Agly Adithya Agly Adithya, Agly Alamsyah Aziz Anas Subarnas Anggia Skynta Ani M Maskoen Anita Fitriaturohmah Annisa Rahmah Furqaani Ardini Saptaningsih Raksanagara Arief Budi Yulianti Armaya Ariyoga Arsita Pratiwi Asep Sukohar Della Erriani Akaputri Devie Ismayanty Dicky Santosa Dida Akhmad Gurnida Djanuarsih Iwan Djanuarsih Iwan, Djanuarsih Djatnika Setiabudi Dwi Prasetyo Eman Sutrisna Enggar Hestu Enggar Hestu, Enggar Fajar Awalia Yulianto Fatmawati Karim Fenda Khafidhotenty Gembong Soeyono Putro Haifa Nur Bilqis Sahnella Hartanto Bayuaji Heda Melinda Nazaruddin Nataprawira Herman Susanto Herry Garna Hidayat Wijayanegara Ida Parwati Ike Rostikawati Husen Ismet Muchtar Nur Julius Gunadi Julius Gunadi, Julius Karimah Karimah Kartika Malahayati Lazuardhi Dwipa Lelly Yuniarti Leri Septiani Lina Herlina M. Nurhalim Shahib Ma&#039;mun Sutisna Ma&#039;mun Sutisna Ma&#039;mun Sutisna Malahayati, Kartika Mamun Sutisna Maya Tejasari Ma’mun Sutisna Meike Rachmawati Meta Maulida Damayanti Miranti Kania Dewi Muayah Muayah MUCHTAN SUJATNO Muhamad Al Hadi A A Muhamad Faishal Ramadhan Muhamad Risdan Hardani Muhamad Risdan Hardani, Muhamad Risdan Muhammad Alief Abdul ‘Aziiz Muhammad Ilham Halim Muthiana Rizky Muthiana Rizky, Muthiana Nabhan Komara Nabhan Komara, Nabhan Nadira Fajriani Nike Arta Puspitasari Nina Susana Dewi Nina Susana Dewi Novita Arya Cahyani Nurhalim Shahib Putro, Gembong Soeyono Ratu Intan Permatasari Robby Hermawan Roni Rowawi Roni Rowawi Rudi Supriyadi Ruswana Anwar Salma Raudhatusabrina Basuki Santun Bhekti Rahimah Selvi Puspan Sari Siska Nia Irasanti Siti Aminah Abdurachman Siti Sugih Siti Sugih Hartiningsih Sri Komalaningsih Suryani Soepadan Suryani Soepardan Suryani Suryani Suwendar Suwendar Tania Novy Tarsikah, Tarsikah Titik Respati Gilarsi Tri M. Andayani Tri Murti Andayani Trully D. Sitorus Trully Detty Sitorus Udin Sabarudin Udin Sabarudin Vasanthi A/P Nayagam WaOde Nur Syuhada Wida Purbaningsih Winni Maharani Wiwiek Setiowulan Yeni Mahwati Yeni Mahwati Yuktiana Kharisma