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ISOLASI, PURIFIKASI PARSIAL DAN KARAKTERISASI ENZIM L-ASPARAGINASE DARI BAWANG PUTIH (Allium sativum) Martina Widhi Hapsari; Windy Rizkiprilisa; Nindi Kusumaningtyas; Novia Anggraeni
Science Technology and Management Journal Vol. 1 No. 2 (2021): Agustus 2021
Publisher : Fakultas Sains dan Teknologi, Universitas Nasional Karangturi

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Abstract

Proses pemanasan yang berlebih pada pengolahan makanan dapat memicu pembentukan akrilamida. Akrilamida merupakan zat beracun yang bersifat karsinogenik pada manusia. Salah satu pencegahan untuk mereduksi akrilamida yaitu dengan penambahan enzim L-asparaginase. Enzim L-Asparaginase dapat mencegah pembentukan akrilamida dengan mengkonversi asam amino L-Asparagin sebagai prekusornya menjadi bentuk asam amino lain yaitu asam L-Aspartat. L-Asparaginase dapat ditemukan pada berbagai organisme salah satunya adalah bawang putih. Tujuan dari penelitian ini adalah mampu mengisolasi dan mengetahui karakter optimum L-Asparaginase dari bawang putih (Allium sativum). Enzim L-Asparaginase dari bawang putih diperoleh melalui beberapa tahap. Tahap pertama yaitu isolasi L-Asparaginase dari bawang putih dan pemurnian melalui pengendapan dengan amonium sulfat dan dialisis. Tahap kedua yaitu uji aktivitas spesifik dan karakterisasi L-Asparaginase dengan cara menghitung jumlah amonia yang terbentuk menggunakan pereaksi Nessler dan memperoleh kadar protein total dengan metode Lowry. Hasil penelitian ini menunjukkan aktivitas spesifik tertinggi LAsparaginase dari bawang putih pada fraksi 5 (80-100%) yaitu 1054,444 U/mg protein. Kondisi optimum L-Asparaginase bebas yaitu pada suhu 37°C, pH 8,6 dan waktu inkubasi selama 30 menit.
POTENSI ACE (ANGIOTENSIN-I CONVERTING ENZYME) INHIBITOR DARI TEMPE KORO – KOROAN SEBAGAI PANGAN FUNGSIONAL ANTIHIPERTENSI Windy Rizkaprilisa; Martina Widhi Hapsari
Science Technology and Management Journal Vol. 2 No. 1 (2022): Januari 2022
Publisher : Fakultas Sains dan Teknologi, Universitas Nasional Karangturi

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Abstract

Koro-koroan dapat diolah menjadi tempe melalui proses fermentasi dengan Rhizopus sp.. Tempe koro berpotensi sebagai pangan fungsional antihipertensi karena menghasilkan peptide ACE inhibitor. Enzim protease memotong protein koro menjadi peptide yang lebih kecil pada proses fermentasi. Setelah melalui proses pencernaan, tempe koro tetap berpotensi sebagai ACE inhibitor karena terjadi proses hidrolisis oleh enzim pencernaan sehingga terjadi pemotongan peptide menjadi peptide yang lebih sederhana dan asam amino. Peptida dengan berat molekul kecil memiliki aktivitas penghambatan ACE yang lebih besar. Proses fermentasi dan hidrolisis pada sistem pencernaan dapat menghasilkan peptide dengan berat molekul kecil sehingga potensi ACE inhibiornya lebih besar.
Pelatihan Pembuatan Makanan Anti Stunting untuk Anak di Kampung Nelayan Tambak Rejo Windy Rizkaprilisa; Martina widhi Hapsari; Novia Anggraeni; Paulus Damar Bayu Murti; Anggara Mahardika
Jurnal Pengabdian Masyarakat (ABDIRA) Vol 2, No 4 (2022): Abdira, Oktober
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v2i4.192

Abstract

This public service activity was order to “Ayo Siaga Bencana Dapur Umum Cegah Stunting” in Kampung Nelayan Tambak Rejo. Kampung Nelayan Tambak Rejo was affected area by flooding due to high tides and beach abrasion, so the childrens can be affected stunting. This program aimed to educate parents about stunting prevention by providing nutritious anti-stunting food and training to make anti-stunting food. This activity consists of three stages: providing education about stunting prevention; training to make anti stunting food and distributing it to the citizens. Parents and children were very enthusiastic when tasted "Bola-bola Ubi Ikan Lele". It were made from nutritious ingredients that were easily to get and affordable price. Education about stunting prevention can be applied in their environment and training about making anti-stunting food can be an idea for food to be given to children.
Pelatihan Pembuatan Snack Bar dari Tepung Ubi Ungu sebagai Alternatif Camilan Sehat Martina Widhi Hapsari; Novia Anggraeni; Paulus Damar Bayu Murti; Anggara Mahardika; Windy Rizkiprilisa
Prima Abdika : Jurnal Pengabdian Masyarakat Vol. 2 No. 3 (2022): Volume 2 Nomor 3 Tahun 2022
Publisher : Program Studi Pendidikan Guru Sekolah Dasar Universitas Flores Ende

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/abdika.v2i3.2065

Abstract

Snacks are often consumed by people to temporarily relieve hunger. These snacks are usually high in sugar and fat and low in fiber. One of the healthy snacks consumed is a snack bar made from local food. In this training, developing a snack bar based on local food ingredients that is low in calories and high in fiber, namely a snack bar based on purple sweet potatoes flour. The purpose of this training is to introduce the community to product diversification using local food from purple sweet potatoes which can be processed into healthy snacks and basic skills on how to make the snack bar. The methods used in this training are lectures, demonstrations and questions and answers. The stages of implementing the activity are an explanation of local food, using purple sweet potato as a basic ingredient for healthy snacks. Next, the practice of making snack bars with the basic ingredients of purple sweet potato flour, peanuts and crispy rice. The results of the evaluation of the snack bar making training, namely the response of the training participants were very satisfied and enthusiastic in participating in this activity because participants could add new insights and knowledge in terms of local food snack bars. In addition, the training participants also got new business ideas to be developed in healthy snack culinary.
Workshop Pengolahan Pembuatan TeKo (Susu Tempe Kopi) pada Acara Research Camp bersama SMA Kebon Dalem Semarang Paulus Damar Bayu Murti; Martina Widhi Hapsari; Novia Anggraeni; Raka Bachtiar Kuspradanarto; Geraldus Tegar Mahardika
Magistrorum et Scholarium: Jurnal Pengabdian Masyarakat Vol 1 No 3 (2021)
Publisher : Universitas Kristen Satya Wacana Salatiga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (882.259 KB) | DOI: 10.24246/jms.v1i32021p417-427

Abstract

This community services activity was addressed at students of SMA Kebon Dalem, Semarang city through a workshop on the processing of making TeKo (coffee tempeh milk) at the Research Camp event. The activity was carried out in the form of training on the processing of making TeKo and being a jury in journal presentations from SMA Kebon Dalem students. Tempe is a local agricultural product from Indonesia which is now worldwide due to its high protein content. The purpose of this service activity is to introduce local products that can be processed into functional drinks. The method used during the service is online through google meet, where the material from the workshop was explained first as an introduction then a TeKo processing demo was carried out. In the service activity, representatives from SMA Kebon Dalem were also invited to taste the results of the TeKo demo. The reception from respondents was very good regarding TeKo which can be used as a functional drink for those who do not like tempe itself.
Workshop Pengolahan Mie Kopi Paulus Damar Bayu Murti; Raka Bachtiar Kuspradanarto; Anggara Mahardika; Martina Widhi Hapsari; Novia Anggraeni; Windy Rizkaprilisa
To Maega : Jurnal Pengabdian Masyarakat Vol 6, No 2 (2023): Juni 2023
Publisher : Universitas Andi Djemma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35914/tomaega.v6i2.1672

Abstract

Mie merupakan jenis pangan yang dapat digunakan sebagai pengganti karbohidrat dari nasi. Biasanya mie ini tergolong sebagai makanan instan karena tidak memerlukan proses memasak yang begitu lama. Pandemi mengajarkan untuk tetap di rumah saja, dan kita dituntut untuk terus kreatif dan inovatif walau di rumah saja. Kegiatan pengabdian masyarakat ini bertujuan untuk memberikan pelatihan pengolahan mie kopi kepada masyarakat khususnya kepada Ibu-Ibu PKK tingkat Kota Semarang, Provinsi Jawa Tengah. Metode yang digunakan dalam kegiatan pengabdian kepada masyarakat ini adalah metode pelatihan demo memasak dilakukan secara daring menggunakan platform zoom meeting. Kegiatan berlangsung dimulai dengan tahapan persiapan, pelaksanaan, tanya jawab, dan pelaporan. Sebanyak kurang lebih 40 orang jumlahnya yang mengikuti kegiatan pelatihan mie kopi ini. Materi yang disampaikan dalam kegiatan workshop mie kopi ini adalah pembuatan mie dari bahan dasar tepung, penggilingan, pembentukan mie, dan proses memasak mie kopi menjadi sebuah hidangan. Kegiatan ini mendapatkan respon positif dari para peserta online yang hadir dengan berbagai pertanyaan disampaikan saat workshop berlangsung. Setelah kegiatan dilaksanakan, diharapkan peserta memperoleh ide inovasi pangan baru dalam bentuk mie kopi.
KARAKTERISTIK HEDONIK DAN GIZI CENDOL IKAN NILA (OREOCHROMIS NILOTICUS) DENGAN PENAMBAHAN KOPI Novia Anggraeni; Windy Rizkaprilisa; Martina Widhi Hapsari; Ratih Paramastuti
Jurnal Borneo Saintek Vol 6, No 1 (2023): Jurnal Borneo Saintek
Publisher : Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/borneo_saintek.v6i1.4200

Abstract

Cendol is one of the popular drinks in Indonesia which is made from flour with incomplete nutritional content, so it is necessary to modify the formulation of cendol by adding tilapia fish meat and coffee grounds to complement the nutritional value of cendol products. The purpose of this study was to determine the effect of the addition of coffee grounds on consumer acceptance and the nutritional value of tilapia cendol products. This study used a completely randomized design (CRD), with 4 treatments adding coffee powder 0 g, 4 g, 6 g, and 8 g. The results showed that the addition of coffee grounds had an effect on the hedonic results of tilapia cendol. The best cendol according to consumer acceptance based on hedonic code K2 results with the addition of 6 g coffee powder with a moisture content of 65.34%, ash content of 0.48%, fat content of 0.52%, protein content of 11.13%, and carbohydrate content of 23 .53%.