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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Indonesian Journal of Business and Entrepreneurship (IJBE) Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil AGRIKAN Jurnal Ilmiah Agribisnis dan Perikanan Prosiding Seminar Nasional Kemaritiman dan Sumber Daya Pulau-Pulau Kecil LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Hibualamo : Seri ilmu-ilmu Alam dan Kesehatan Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil GANESHA: Jurnal Pengabdian Masyarakat Agrikan: Jurnal Agribisnis Perikanan HIRONO : Jurnal Pengabdian Masyarakat
Febrina Olivia Akerina
Fakultas Sains, Teknologi Dan Kesehatan, Universitas Hein Namotemo, Tobelo
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Public Health Social Sciences Veterinary

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Journal : Jurnal Pengolahan Hasil Perikanan Indonesia

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Isolation and Characterization of Antibacterial Compounds from Sea Urchin Febrina Olivia Akerina; Tati Nurhayati; Ruddy Suwandi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 18 No 1 (2015): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3760.038 KB) | DOI: 10.17844/jphpi.v18i1.9564

Abstract

Sea urchin is highly valued seafood. Its bioactive compound also has a potential as natural antibacterial agent. The aim of this research was to screen the antibacterial activity using cup-plate method, toxicity potential using brine shrimp lethality test (BSLT) method, bioactive compound using phytochemical method, and proximate composition by AOAC. This research was devided into two phases, the preliminary research was to determine the best body part of sea urchin showing antibacterial activity. Sea urchins were collected from Pramuka Island and extracted by meseration method. For the preliminary research, gonad extract showed the high antibacterial activity against E. coli and S. aureus at 1.83 ± 0.74 mm and 1.5 mm, respectively. The main research includes the determination of proximate composition, toxicity, bioactive compound, and antibacterial activity from the best body part of sea urchin. The proximate composition from gonad of sea urchin showed that water content 64.97 ± 0.08%; ash 2.72 ± 0.13%; lipid 19.73 ± 0.04%; protein 12.26 ± 0.3%, and 0.33 ± 0.17%, respectively. The detected bioactive compounds from the three different solvents of gonads extracts were steroid, triterpenoid and saponin. The result of lethal toxicity (LC50) from the three gonads extract was 471.861 ppm (n-hexane), 563.226 ppm (ethyl acetate) and 577.531 ppm (methanolic), respectively. Gonads ethyl acetate extracts showed the highest antibacterial activity than n-hexane and methanolic extract, its inhibition zone was 2.71 mm against S. aureus and 4.13 against E. coli. Keyword : Antibacteria, bioactive compound, D. setosum, sea urchin
Co-Authors Angga Rivanly Kitong Anggeline Amahorseja Anita Dilly Femsy Kour Femsy Kour Hendriane Namotemo Janer Sangaji Kadek Intan Dwi Anggari Kour, Femsy Rendy Simange Riki Rikardo Ada Ruddy Suwandi TATI NURHAYATI Zakarias Dilago