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All Journal BIOMA Universa Medicina
Nugrahaningsih Nugrahaningsih
Semarang State University
Agriculture, Biological Sciences & Forestry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Andrographis paniculata extract induced apoptosis of adenocarcinoma mammae in C3H mice Nugrahaningsih Nugrahaningsih; Sarjadi Sarjadi; Edi Dharmana; Hartanto Wahyu Subagio
Universa Medicina Vol. 32 No. 2 (2013)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2013.v32.99-107

Abstract

BACKGROUNDApoptosis plays an important role in tumorigenesis. Induction of apoptosis is a strategy for developing cancer therapy. In vitro study found that andrographolide isolated from Andrographis paniculata has anticancer activity by an apoptotic mechanism in cancer cell lines. The aim of the present study was to prove theeffect of Andrographis paniculata extract administered orally on apoptosis of mammary adenocarcinoma in C3H mice.METHODSThis study was of post test randomized control group design. Twenty four C3H mice with transplanted mammary adenocarcinomas were divided into four groups. To three groups Andrographis paniculata extract was administered orally for14 days, at doses of 5, 10 and 15 mg/day, respectively, whereas to the control group no Andrographis paniculata extract was administered. On day 15 the mice were terminated. The mammary adenocarcinomas were examined by the terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) method. The values of the apoptotic index were expressed as mean±SD and analyzed using ANOVA and Pearson’s correlation test.RESULTSThe mean apoptotic index values differed significantly among the experimental groups (p=0.001). The highest value was found in the group receiving Andrographis paniculata extract 15 mg/day, while the lowest was in the control group, the values being significantly correlated (r=0.974).CONCLUSIONSOral administration of Andrographis paniculata extract induced apoptosis in C3H mice with mammary adenocarcinoma
Association of TCF7L2 Gene Polymorphism With Non Obesetype 2 Diabetes Mellitus in Javanese Population Ari Yuniastuti; R Susanti; Nugrahaningsih Nugrahaningsih; Rahmatika Saputri R
Bioma : Jurnal Ilmiah Biologi Vol 12, No 2: October 2023
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v12i2.15365

Abstract

Type 2 diabetes is characterized by insulin deficiency due to inadequate insulin secretion or decreased tissue response to insulin at one or more points. Three important factors that influence the pathogenesis of type 2 DM are individual factors or ethnic genetics, factors of pancreatic β cell damage and insulin resistance factors. The TCF7L2 gene is a gene that codes for TCF7L2 protein, a group of proteins that act as transcription factors in the Wnt signal pathway. TCF7L2 gene polymorphism occurs when cytosine is replaced with thymine in the intron 3 position in wild-type due to alternative splicing.The purpose study was to determine the frequency of TCF7L2 gene polymorphism in Javanese type 2 non-obese DM patients in Semarang. Detection of TCF7L2 gene polymorphism was carried out on 49 people with type 2 non-obese DM patients and 38 people not with type 2 DM using the PCR-RFLP method. The restriction enzyme used is Rsa1. The allele frequency of TCF7L2 gene polymorphism was analyzed by Chi-square and analysis of sample characteristics was carried out with Odds Ratio. The results showed that there were significant differences in the allele frequency of TCF7L2 gene polymorphism and in the characteristics of age, KGD and systole between case subjects and control subjects. From this study it was concluded that TCF7L2 gene polymorphism is associated with the type 2 DM through interactions between genetic factors (T allele) and life style.
Co-Authors Ari Yuniastuti Edi Dharmana Hartanto Wahyu Subagio R Susanti Rahmatika Saputri R Sarjadi Sarjadi