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PENGARUH PEMBERIAN MADU KELENGKENG TERHADAP AKTIVITAS ENZIM SUPEROXIDE DISMUTASE DAN KATALASE PADA TIKUS YANG DIINDUKSI Pb Asetat Ari Yuniastuti; Kamilatussainah Kamilatussainah; Fitri Arum Sasi
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2015): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 6 2015
Publisher : Prosiding SNST Fakultas Teknik

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Abstract

Penelitian ini bertujuan untuk mengetahui  aktivitas enzim antioksidan superoksida dismutase (SOD) dan katalase dalam plasma tikus wistar yang diiunduksi Pb asetat dan suplementasi madu kelengkeng. Penelitian eksperimental laboratorik, dengan rancangan The Post Test-Only Group Designdilakukan pada bulan Maret 2015 menggunakan hewan coba tikus putih wistar umur 2-3 bulan dengan berat badan 150-250. Sebanyak 20 ekor tikus diadaptasi dan diberi pakan standar selama 2 minggu. Selanjutnya dibagi secara acak menjadi 4 kelompok, yaitu : kelompok kontrol hanya diberi pakan standar,  K(-): kelompok tikus yang diberi perlakuan Pb asetat 10 mg/kgBB, P1 : kelompok tikus yang disuplementasi madu kelengkeng 0,9 ml/200 grBB dan Pb asetat 10 mg/kgBB, P2: kelompok tikus yangdisuplementasi madu kelengkeng 1,8 ml/200 grBB dan Pb asetat 10 mg/kgBB dilakukan selama 14 hari. Setelah akhir perlakuan tikus diambil darahnya melalui plexus retroorbitalis untuk diukur kadar SOD dan CAT.  Hasil pemeriksaan dianalisis dengan uji Chi Square dengan batas signifikansi = 0,05.Hasil penelitian menunjukkan bahwa pada tikus kelompok kontrol kadar SOD 141,50μ/ml dan CAT 0,8μ/ml; K(-) kadar SOD 77,71μ/ml dan CAT 0,12μ/ml; P1 kadar SOD 289,82 μ/ml dan CAT 1,21. Sedangkan pada tikus P2,kadar SOD 391,59 dan kadar CAT 3,62. Secara statistik terdapat perbedaan signifikan antar kelompok. Kata kunci: antioksidan, katalase, madu kelengkeng, superoksid dismutase
The Effect Of Applying Guided Discovery Learning (GDL) Model on the Students' Critical Thinking Abilities In Budidaya Lele (BDL) Subject Grade 11 SMA N 10 Semarang Amalia, Devi; Yuniastuti, Ari; Isnaeni, Wiwi
Journal of Biology Education Vol 8 No 3 (2019): December 2019
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v8i3.27015

Abstract

SMA Negeri 10 Semarang is a school in Semarang which applies Budidaya Lele (BDL) subject. This subject is in accordance with the learning reference in the 2013 Curriculum. One of the goals to be achieved in the 2013 Curriculum is improving students’ critical thinking abilities. However, the facts show that students’ critical thinking abilities in SMA Negeri 10 Semarang are still low. One factor causing the low level of critical thinking abilities is conventional learning model implementation and teacher-centered. One of the learning models that can help improve students’ critical thinking abilities is Guided Discovery Learning (GDL). This study aims to investigate the effect of GDL model on students’ critical thinking abilities of grade 11 science in BDL subject. The sampling technique was purposive sampling. The research object was students in 11 science 4 class as the experimental class applying GDL model and students in 11 science 3 class as the control class applying conventional learning model. The aspects of critical thinking abilities measured were ability to provide simple explanation, ability to develop basic skills by using the indicators of considering trusted sources, ability to draw conclusion, ability to provide further explanation, and ability to manage strategies and tactics. The data was collected through tests and questionnaires. The data of test results was analyzed by using T test using SPSS and N-Gain test in Microsoft Excel 2010, while the questionnaire data was descriptively analyzed. The data analysis result of T test shows a mean difference in the experiment class and control class indicated by sig. value (2-tailed) as much as 0.000. The result of N-gain test, the experimental class has 0.43 value with medium criteria and the control class has 0.24 value with low criteria. The results of the two tests show that GDL model implementation affects students’ critical thinking abilities in the experimental class in BDL lesson. Students in the experimental class applying GDL model have better critical thinking abilities compared to students in the control class which does not apply GDL model.
MATHEMATICAL MODEL OF' GAULTHERASE INACTIVATION KINETICS FOR THE GAULTHERIN PRODUCTION FROM WINTERGREEN (Gaulteria fragantissima) Yulianto, Mohamad Endy Y; Hartati, Indah; yuniastuti, Ari
METANA Vol 5, No 2 (2007): Desember 2007
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6261.26 KB) | DOI: 10.14710/metana.v5i2.1905

Abstract

Abstract Wintergreen (Gaultheria fragantissima) is the source of the essential oil of wintergreen which is comprised predominantly of methyl salicylic. During this time wintergreen is not yet economically developed because of the lack of the cultivation technology. One of effort that is needed to be studied beside the cultivation tecnology and  the wintergren oil quality development is the product diversification. wintergreen comprised by far the highest concentration of both free salicylic and total salicylic acid which is expected of a plant known for the formation of the wintergreen oil, an essential oil consisting primarily of methyl salicylic. The concentration of total salicylate found in wintergreen is over 20 fold greater than the total salicylic concentration (salicylic and any derivates combined) found in Filipendula, and 100 fold greater than that found in Lemon Thyme. The active form of salicylic acid in wintergreen is Gaultherin. Gaultherin has many properties related to human health. Gaultherin consists of methyl salicylic conjugated to the disaccharide, primeverose. When plant tissues are disrupted the endogenous gaultherin is rapidly lost, presumably by enzymatic hydrolysis with the release of methyl salicylic. The problem is until this time there is not yet available an effective method for the gaultherin extraction. The difficulties faced on the gaultherin production are the fact that along the gaultherin extraction. and along with the disruption of the wintergreen tissue, the gaultherin is hydrolyzed into its individual components which are methyl salicylic and disaccharide. The alternative process for the gaultherin production from wintergreen is the gaultherin production process by enzyme inactivation and  extraction process in an inactivation extractor using a n organic solvent (alcoholic solvent extraction). This paper is intended to study the enzyme inactivation rate which is play an important role in the dimension determination of the process equipments. Enzyme inactivation can be describes as first orde reaction. The enzyme inactivation constant  kp is the temperature, pressure, and water concentration function. The dependency, model of  the temperature based on Arhenius type while the pressure dependency  is based on Eyring equation.   Keywords : gaultherin, inactivation. kinetics
Effectiveness of Tera Gymnastics and Healthy Heart Gymnastics on Blood Pressure Among Elderly With Hipertension in Sinomwidodo Village, Tambakromo Subdistrict, Pati District Rofika, Ana; Yuniastuti, Ari
Public Health Perspective Journal Vol 3, No 1 (2018): April 2018
Publisher : Universitas Negeri Semarang

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Abstract

The elderly are at high risk for degenerative diseases. Hypertension occupies the largest proportion of all degenerative diseases reported. Gymnastics Tera and Healthy Gymnastics are one of the nonpharmacology efforts to suppress Hypertension. The purpose of this study was to determine the effectiveness of Gymnastics Tera and Healthy Gymnastics Against Blood Pressure on Hypertensive Elderly. This research uses a quasi experiment method with Nonequivalent control group design. The population in this study is all elderly who suffer from Hypertension in Sinomwidodo Village Tambakromo Subdistrict, Pati Regency which according to criteria amounting to 35 elderly. Sampling with Purposive Sampling technique and sample size is determined by Slovin formula. The sample is 32 respondents with the provision of 11 people tera gymnastics group, 11 people healthy heart gymnastic group and 1o people control group. The results showed that after the intervention there was a significant effect between the group of Gymnastics Tera and Healthy Gymnastics compared with the control group with the measurement of mercury p-value (<0.05). There was no significant difference from the comparison of the post-test value of gymnastic exercise of Tera and Healthy Heart gymnastic to systolic and diatolic blood pressure in elderly hypertension p-value (> 0.05). Conclusion Gymnastics Tera and Healthy Gymnastic Gym are effective in lowering blood pressure in hypertensive elderly. The effectiveness of gymnastics Tera and Healthy Heart gymnastics are the same.
ANALISIS SEKUEN GEN GLUTATION PEROKSIDASE (GPX1) SEBAGAI DETEKSI STRES OKSIDATIF AKIBAT INFEKSI MYCOBACTERIUM TUBERCULOSIS Yuniastuti, Ari; Susanti, R.
Sainteknol : Jurnal Sains dan Teknologi Vol 11, No 2 (2013): December 2013
Publisher : Unnes Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v11i2.5569

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Glutation merupakan antioksidan yang berperan dalam fungsi imun, dan diekspresikan secara genetik oleh urutan gen yang membentuk protein enzim Glutation Peroxidase (GPx1). Bila ekspresi gen berubah maka terjadi perubahan fungsi glutation dan kerentanan terhadap stress oksidatif. Metode yang digunakan adalah Kasus-kontrol. Sampel yang digunakan adalah sampel darah. Kelompok kasus adalah sampel darah pasien tuberkulosis paru sedangkan kelompok kontrol adalah sampel darah orang sehat. Pemeriksaan gen Glutation peroxidase (GPx1) menggunakan metode Polymerase Chain Reaction (PCR) untuk melihat pita DNA pada pasien tuberkulosis par serta elektroforesis produk PCR-RFLP gen GPx1 kelompok sampel tuberkulosis. Hasil penelitian menunjukkan bahwa tidak terdapat hubungan yang bermakna antara polimorfisme gen GPx1 (p=0,365) pasein tuberkulois dengan individu sehat, sehingga tidak dapat digunakan sebagai alat deteksi kerentanan terhadap stress oksidatif pada pasien tuberkulosis. Perlu penelitian lanjutan yang menggunakan sampel lebih besar dan populasi etnik yang berbeda.
IDENTIFIKASI APOPTOSIS DENGAN METODE TUNEL PASCA PEMBERIAN EKSTRAK SAMBILOTO DAN PENGARUHNYA TERHADAP VOLUME TUMOR WH, Nugrahaningsih; Yuniastuti, Ari
Sainteknol : Jurnal Sains dan Teknologi Vol 13, No 1 (2015): June 2015
Publisher : Unnes Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v13i1.5335

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Penelitian ini dilakukan untuk membuktikan pengaruh ekstrak sambiloto terhadap apoptosis dan volume tumor bila diberikan secara oral dan diidentifikasi dengan metode TUNEL. Penelitian dilakukan dengan desain Randomized post test control group. Sebanyak 24 ekor mencit C3H yang telah tumbuh kanker mamma dibagi secara acak menjadi 4 kelompok masing-masing 6 ekor. Diberikan ekstrak sambiloto dengan dosis 5, 10 dan 15 mg per ekor per hari, dan kelompok kontrol. Pemberian ekstrak sambiloto dilakukan secara oral selama 14 hari. Pengukuran volume tumor dilakukan pada hari ke 1, 4, 8, 12 dan 15. Pada hari ke 15 mencit dimatikan dengan menggunakan eter. Jaringan kanker dibuat blok parafin untuk pemeriksaan apoptosis dengan metode TUNEL. Hasil Penelitian menunjukkan adanya peningkatan signifikan sel apoptosis (p=0,000), dengan dosis terbaik 15 mg/hari. Ada beda yang bermakna pada rerata kelipatan penambahan volume tumor (p=0,049). Hasil uji post hoc menunjukkan bahwa perbedaan terletak antara kelompok kontrol dengan kelompok dosis 10 mg/hr. Dapat disimpulkan bahwa pemberian ekstrak sambiloto secara oral meningkatkan sel yang mengalami apoptosis dengan pemeriksaan TUNEL namun belum dapat menurunkan volume tumor.
IDENTIFIKASI APOPTOSIS DENGAN METODE TUNEL PASCA PEMBERIAN EKSTRAK SAMBILOTO DAN PENGARUHNYA TERHADAP VOLUME TUMOR Nugrahaningsih, Nugrahaningsih; Yuniastuti, Ari
Sainteknol : Jurnal Sains dan Teknologi Vol 12, No 2 (2014): December 2014
Publisher : Unnes Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v12i2.5415

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Apoptosis adalah kematian sel terprogram (programed cell death) yang bertujuan untuk mempertahankan kestabilan populai sel. Kegagalan pengaturan apoptosis dapat menyebabkan sel membelah tanpa terkendali, yang disebut sebagai kanker. Peningkatan apoptosis merupakan suatu upaya yang dikembangkan sebagai terapi kanker. Pemeriksaan apoptosis dengan metode TUNEL(Terminal deoxyribonucleotidyl transferase dUTP nick end labeling) dapat memberikan gambaran proses apoptosis pada tingkat sel tunggal sehingga lebih spesifik dan memiliki akurasi tinggi. Sambiloto merupakan salah satu tanaman obat tradisional yang digunakan sebagai anti kanker. Beberapa penelitian yang dilakukan secara in vitro menunjukkan pengaruh andrografolid yang diisolasi dari sambiloto terhadap apoptosis sel kanker pada kultur. Hasil tersebut masih memerlukan penelitian lebih lanjut, terutama pengaruhnya bila diberikan secara oral. Penelitian ini dilakukan untuk membuktikan pengaruh ekstrak sambiloto terhadap apoptosis dan volume tumor bila diberikan secara oral dan diidentifikasi dengan metode TUNEL. Penelitian dilakukan dengan desain Randomized post test control group. Sebanyak 24 ekor mencit C3H yang telah tumbuh kanker mamma dibagi secara acak menjadi 4 kelompok masing-masing 6 ekor. Diberikan ekstrak sambiloto dengan dosis 5, 10 dan 15 mg per ekor per hari, dan kelompok kontrol. Pemberian ekstrak sambiloto dilakukan secara oral selama 14 hari. Pengukuran volume tumor dilakukan pada hari ke 1, 4, 8, 12 dan 15. Pada hari ke 15 mencit dimatikan dengan menggunakan eter. Jaringan kanker dibuat blok parafin untuk pemeriksaan apoptosis dengan metode TUNEL. Hasil Penelitian menunjukkan adanya peningkatan signifikan sel apoptosis (p=0,000), dengan dosis terbaik 15 mg/hari. Ada beda yang bermakna pada rerata kelipatan penambahan volume tumor (p=0,049). Hasil uji post hoc menunjukkan bahwa perbedaan terletak antara kelompok kontrol dengan kelompok dosis 10 mg/hr. Dapat disimpulkan bahwa pemberian ekstrak sambiloto secara oral meningkatkan sel yang mengalami apoptosis dengan pemeriksaan TUNEL namun belum dapat menurunkan volume tumor.
PERAN RECIPROCAL TEACHING KOMIK TERHADAP MOTIVASI DAN HASIL BELAJAR SISWA Raida, Sulasfiana Alfi; Yuniastuti, Ari; Dewi, Pramesti
Journal of Biology Education Vol 1 No 1 (2012)
Publisher : Journal of Biology Education

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Abstract

PENGARUH MODEL GUIDED INQUIRY BERBANTUAN FISHBONE DIAGRAM TERHADAP MOTIVASI DAN HASIL BELAJAR SISWA Dewayanti, Mayang Siwi; Yuniastuti, Ari; Prasetyo, Andreas Priyono Budi
Journal of Biology Education Vol 4 No 2 (2015)
Publisher : Journal of Biology Education

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Abstract

Penelitian ini bertujuan untuk menguji pengaruh model guided inquiry berbantuan fishbone diagram terhadap motivasi dan hasil belajar siswa pada materi sistem pencernaan. Penelitian ini menggunakan rancangan Quasi Experimental Design dengan menggunakan pola non randomized control group pretest-posttest design. Populasi dalam penelitian ini adalah seluruh siswa kelas VIII SMP Negeri 1 Ambarawa, sedangkan sampel dalam penelitian ini adalah siswa kelas VIII B (kelas eksperimen) dan kelas VIII C (kelas kontrol). Data keterlaksanaan model guided inquiry berbantuan fishbone diagram diperoleh melalui angket tanggapan yang diisi oleh siswa. Data hasil belajar dalam ranah kognitif siswa dikumpulkan dari tes materi sistem pencernaan, ranah psikomotorik dan ranah afektif diperoleh melalui lembar observasi. Data motivasi belajar siswa diperoleh melalui angket skala psikologi siswa. Hasil uji hipotesis pengaruh model guided inquiry berbantuan fishbone diagram terhadap motivasi belajar siswa diperoleh nilai χ2= 32,00 dengan p &lt; 0,05 (p = 0,010) dan pengaruh model guided inquiry berbantuan fishbone diagram terhadap hasil belajar siswa diperoleh χ2= 20,67 dengan p &lt; 0,05 (p = 0,014). Hal ini menunjukkan bahwa model guided inquiry berbantuan fishbone diagram berpengaruh terhadap motivasi dan hasil belajar.   This research aimed to examine the effect of guided inquiry models with fishbone diagram on students’s motivation and learning achievement on the subject of digestive system. This research was Quasi Experimental Design. The design of this research was non randomized control group pretest posttest. The population in this research was all students, VIII grade class at SMP Negeri 1 Ambarawa, VIII B grade class was treated as experimental class and VIII C as control class. Questionare was used to collect data of  the implementation level guided inquiry models with fishbone diagram. Test was used to collect data of students learning achivement on cognitive, psychomotoric domains while affective domains were  collected by observation. Psychology scale was used to collect data of students motivation. The hypothesis result of the influence of guided inquiry models with fishbone diagram toward upon students learning motivation was χ2= 32,00 with p &lt; 0,05 (p = 0,010) and the influence guided inquiry models with fishbone diagram toward students learning achievement was χ2= 20,67 with p &lt; 0,05 (p = 0,014). The model guided inquiry with fishbone diagram toward had significant effect upon students’ learning motivation and achievement.
PENGEMBANGAN OLAHAN HASIL TERNAK MELALUI DIVERSIFIKASI PRODUK BERBAHAN DASAR SUSU DI KELURAHAN CEPOKO GUNUNGPATI SEMARANG Yuniastuti, Ari; Setiati, Ning; Susanti, R
Rekayasa : Jurnal Penerapan Teknologi dan Pembelajaran Vol 15, No 1 (2017): Juli 2017
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/rekayasa.v15i1.12561

Abstract

Kelurahan Cepoko is one of 16 urban villages in Gunungpati sub-district. The source of income of Cepoko village residents is in the field of livestock, as much as 77 residents of Cepoko urban village who rely their life in the field of farms, both chicken farms, dairy cows and meat cows. Dairy cattle breeding activities do not provide sufficient income, because the selling price of milk purchased by cooperatives is too cheap, so it can not cover operational costs. Therefore, one of the citizens of SME RW 09 kelurahan Cepoko that is Ibu Ismiyati took the initiative to make processed foods made from milk. Mrs. Ismiyati as a pioneer of milk sticks production business that is personal with employ 5% of women PKK group. Dairy products that have been done just limited to milk stick. Though dairy products have the potential to be developed in order to increase farmers’ income. One of the obstacles to the development of dairy products is the lack of mastery of information on production and marketing techniques by mothers, the limited knowledge and skills of mothers in the processing of milk into products worth selling, causing dairy products are less varied and not devel- oped. The methods undertaken to improve knowledge on milk-based food production techniques are socialization, training and monitoring. PKK ladies are very enthusiastic to follow the milk process- ing into a variety of food products that are proclaimed with the presence of mothers despite the rain, they still spend time attending training activities. PKK ladies become aware of the diversification of products based , and skilled in processing milk into various foods as well as skilled in the produc- tion manejemn such as kenmas and marketing processed products kamanan Participants want more follow-up is more real in terms of assistance and continuous monitoring and marketing of dairy- based food products broadly and poured in MoU between FMIPA and Gunungpati District
Co-Authors Abdurrahman Ali, Nur Aini Abudu, Komaria Ode Adevia Maulidya Chikmah, Adevia Maulidya Adhisti Lutfia Hanan Adiba, Daniar Arof Adiba, Daniar Arof Aennie Widiastuti, Vinka Afniyati, Nur Afniyati, Nur Agnes Dwi Safarina Aidah, Nur Aisa Nikmah Rahmatih Amalia, Devi Andreas Priyono Budi Prasetyo Ariyanto, Andhika Arum Kartikadewi Arum Kartikadewi Arum Kartikadewi Asih Kuswardinah Asyumdah, Asyumdah Budi Laksono Budi, Dini Wurnaning Budu - Candrawan, Roni Prasetyo Devi Dwi Jayanti Dewi Mustikaningtyas Dyah Rini Indriyanti Elissa, Intan Ayu Ell Khaq, Arrina Ely Rudyatmi, Ely Eunike Raffy Rustiana Fathnin, Fildza Huwaina Feri Dwi Agustina Fidia Fibriana Fitri Arum Sasi, Fitri Arum Halimah, Khori Handayani, Kasmini Hidayah, Arifatul Hidayah, Salis Nur I Wayan Suardana Ibnul Mubarok Indah Hartati Innayah, Nurul Irawan Yusuf Isnaeni, Lili Astuti Isnaeni, Lili Astuti Isti Mulyawati Istianah Istianah Istiqomah Dwi Andari, Istiqomah Dwi Istiqomah, Annisa Aulia Jiwanjaya, Yoga Juwita, Fitra Kamala Nuriyah Kamilatussainah Kamilatussainah Kamisih, Kamisih Karomika, Ati Kasimi, Oktia Woro Khikmatul Maula, Lia Kirana Rannoe Tirta Krispinus Kedati Pukan lathifah, Ummu Lindasari, Pamela Lisdiana Lisdiana Lyndasari, Pamela Mahalul Azam Mayang Siwi Dewayanti, Mayang Siwi Merzistya, Aufiena Nur Ayu Mohamad Endy Y Yulianto Muchamad Dafip Muchamad Dafip Muh. Nasrum Massi Nabila Azzahra Luthfiah Nadhifah, Shiliya Lana Nadhifah, Shiliya Lana Nbaia, Sara M Mohamed Negarani, Eryca Nur Bela Negarani, Eryca Nur Bela Niken Subekti Ning Setiati Nirmala Al Fatiah Noor Aini Habibah Nugrahaningsih Nugrahaningsih Nugrahaningsih Nugrahaningsih Nugrahaningsih W H Nugrahaningsih WH, Nugrahaningsih Nur Kusuma Dewi Nur Rahayu Utami NURUL HIDAYAH Oktia Woro Oktia Woro Kasmini H Oktia Woro Kasmini Handayani Oktia Woro Kasmini Handayani Pramesti Dewi Pramusinta, Linda Prasetyowati, Tri Ari Priyantini Widiyaningrum R Susanti R Susanti R Susanti R Susanti R Susanti R Susanti R Susanti R. Susanti R. Susanti Rahayu, Rr. Sri Ratna Rahmatih, Aisa Nikmah Ratna Rahayu, Sri Ratna Rahayu, Sri Retno Sri Iswari Retno Sri Iswari Rofika, Ana Rohmah, Amalia Nor Rr. Sri Ratna Rahayu Sa’adah, Siti Rofiatus Saiful Ridlo Satriani Satriani Sekar Arum, Vriska Roro Setyoko Setyoko Sholikah, Anik Sholikah Shovrotul Khoiriyah, Ely Siti Alimah Siti Baitul Mukarromah Siti Isnaeni SITI MAHMUDAH Sri Mulyani Endang Susilowati, Sri Mulyani Sri Ratna Rahayu, Rr Sulasfiana Alfi Raida, Sulasfiana Alfi Sulistiani, Duwi Sulistyawati, Anita Syarifah, Aidah Nur Talitha Widiatningrum Totok Sumaryanto Florentinus, Totok Sumaryanto Tyas Agung Pribadi Umam, Syaiful Ishlahul Utami, Hanna Dewi Utami, Hanna Dewi Virtanti, Uji Wahyuningtyas, Arini Tri Widya Harry Cahyati Wiwi Isnaeni Woro K H, Oktia Yanti, Erny Yuni Yanuarita Tursinawati Yanuarita Tursinawati Yanuarita Tursinawati Yanuarita Tursinawati Yuni Wijayanti yuniarti, christina ary Zunikhah Zunikhah