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The Potency and Quality of Goatâs Semen for Technological Application of Artificial Insemination Pamungkas, Fitra Aji
Indonesian Bulletin of Animal and Veterinary Sciences Vol 19, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

The productivity of local goat is still relatively lower compared to that of other breed for sub-tropic area. Efforts for increasing its productivity through crossbreeding with genotypes goat could be approached by technological application of artificial insemination (AI). In supporting this technology, the viability of semen for both quality and quantity is needed. Evaluation of Indonesian goat semen characterisation shows a potency for frozen semen. The survive ability of sperm in fresh semen is very limited therefore reducing the temperature to -5Â°C (chilled semen) or -196Â°C (frozen semen) could be done to maintain its survive ability. Optimalization of frozen semen could be done by diluting in Tris extender with 6% glycerol, equilibrating for 4 hours and cooling for 4 â 5 minutes above surface of LN2 before stored in LN2 (-196Â°C). Thawing at > 7Â°C for 30 seconds resulted in the highest percentage of mortility (52.0%) and survivability (65.03%). Chilled semen is the best alternative for artificial insemination (AI) in the field condition where the supply of container and liquid nitrogen are limited. The survivability of chilled semen could be maintained for 8 days and the highest percentage of pregnancy resulted from chilled semen stored up to 24 â 48 hours.Â Key words : Goat, semen quality, frozen semen, chilled semen, artificial insemination

The Use of Vitrification Method For Cryopreservation of Mammalian Oocyte Pamungkas, Fitra Aji
Indonesian Bulletin of Animal and Veterinary Sciences Vol 20, No 3 (2010)
Publisher : Indonesian Animal Sciences Society

Technique cryopreservation of oocyte is a way to storage, maintenance, and guarantee the survival of frozen cells. Vitrification is a cryopreservation method which is increasingly popular in reproduction but it is still difficult to be done because of the size, shape, and numbers of oocytes, as well as osmotic shock and fractures. The efforts to improve the method and technique vitrification are by reducing the concentration of cryoprotectants, increasing the cooling rate and warming, recovery of meiotic spindles, and the time of fertilization. Vitrification solution consist of 15% (v/v) ethylene glycol, 15% (v/v) dimethylsulfoxide or 1,2-propanediol, and 0.5 mol/L sucrose was less toxic. Therefore, at 37Â°C, 2 â 3 minutes are usually used for the pretreatment solution and 20 â 30 seconds for exposure to the vitrification solution. In contrast, at room temperature, 5 â 15 minutes are commonly used for pretreatment and 30 â 60 seconds for exposure to the vitrification solution. Warming procedure is performed by direct immersion of the straw into a water bath. Holding the straw in air for 5 seconds before immersion can avoid bursting or performed warming and dilution at 37Â°C. While the time of fertilization performed at 2 â 3 hours after thawing and incubation for oocyte spindle to recover which is essential for the successful of oocyte cryopreservation program. Key words: Cryopreservation, vitrification, oocyte

Cauda Epididymis Spermatozoa: Cryopreservation and Utilization for Artificial Insemination and In Vitro Fertilization Pamungkas, Fitra Aji
Indonesian Bulletin of Animal and Veterinary Sciences Vol 22, No 4 (2012)
Publisher : Indonesian Animal Sciences Society

Genetic material either from animals of economical interest or from wildlife conservation can be lost anytime by unexpected death of the animal, low libido, or disorder at reproduction. In this case, an effort can be made occur to avoid the total lost of that genetic material by using an epididymis spermatozoa. Cauda epididymis spermatozoa generally motile, mature and can be used to fertilize oocytes as well as ejaculated spermatozoa. Some research indicated that cryopreservation of cauda epididymis spermatozoa for the purpose of artificial insemination and in vitro fertilization showed the ability to fertilize oocytes and produce offspring. Key words: Spermatozoa, cauda epididymis, artificial insemination, in vitro fertilization

Estimated analysis criteria of hatched weight and body weight 12 weeks of Kampung chicken selection Pamungkas, Fitra Aji
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 4 (2005)
Publisher : Indonesian Animal Sciences Society

Genetic parameter estimation for production traits are important in designing genetic selection program for Kampung chicken. The aimed of this research is to study heritability, accuracy of selection, and phenotypic and genotypic correlation of hatched weight and body weight at 12 weeks of Kampung chicken. Five hundred and fourteen head of Kampung chicken consist of 13 cocks, 65 hens, and 436 chicks were used in this study. Nested design analysis were used as described by Becker. The heritability estimation of hatched weight was calculated based on paternal half-sib, maternal half-sib, and full-sib corelation and itâs values were 0.35, 0.37, and 0.36 respectively. Heritability of body weight at 12 weeks based on paternal half-sib, maternal half-sib, and full-sib corelation were 0.27, 0.18, and 0.22 respectively. Selection accuracy of hatched weight were 59-61%, and selection accuracy of body weight at 12 weeks were 42 up to 52%. Genotypic and phenotypic correlation of hatched weight and body weight at 12 weeks estimation based on paternal half-sib, maternal half-sib, full-sib corelation were 0.29, 0.78, 0.51, and 0.17 respectively, indicated selection on one trait will affected the response on other traits positively. Â Â Key Words: Heritability, Selection, Kampung Chicken

Cryopreservation of Boer goat spermatozoa: Comparison of two freezing extenders based on post-thaw sperm quality and fertility rates ., Anwar; Pamungkas, Fitra Aji; Batubara, Aron
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 2 (2014)
Publisher : Indonesian Animal Sciences Society

Boer goat have recently been popularly used for cross breeding with local goats. However, it is currently considered a breed at very limited number with relatively high prices . In this context, the cryopreservation of spermatozoa is important because it could be conserved for a very long period of time. Egg yolk extenders are most commonly used for cryopreservation of goat sperm. The aim of this study was to compare the ability of two extenders to maintain sperm viability after cryopreservation. Semen from three male Boer goat aged about 2-3 years old was collected using artificial vagina and frozen with Tris and Triladyl extender. The results showed that percentage of motility, viability and membrane integrity of spermatozoa with Tris and Triladyl extenders at every stage of cryopreservation showed not significantly difference (P>0.05), except the percentage of sperm motility post thawing of Triladyl was higher than Tris extender (52.00Â±4.47% vs 47.50Â±2.74%, P<0.05). Cryopreserved semen in Tris extender provided the same fertility rates after cervical insemination compared to Triladyl (62.50% vs 60.00%). In conclusion, the Tris extender has the same capabilities to Triladyl in cryopreservation of Boer goat spermatozoa as to maintain sperm quality and fertility rates. Key Words: Boer Goat, Spermatozoa, Cryopreservation, FertilityÂ

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