Krisna N.A. Pangesti
Basic Technology Center for Biomedical and Health, National Institute of Health and Research and Development

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Antibody anti-H5N1 detection in poultry farmers and workers in poultry collection facilities in Indonesia, 2007 Setiawaty, Vivi; Sedyaningsih, Endang R.; Sudiro, Tjahyani M.; van Beest Holle, Mirna Robert D.R.; Pangesti, Krisna N.A.; Ibrahim, Fera
Medical Journal of Indonesia Vol 19, No 2 (2010): May
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.071 KB) | DOI: 10.13181/mji.v19i2.394

Abstract

Aim Between July 2005 and May 2008, Indonesia reported 133 H5N1 confirmed human cases with a case fatality proportion of 81%. Fifty-four percent of cases had a history of direct contact with poultry (chickens). Therefore, it is important to define the detection of antibody of H5N1 among people who have intensive contact with poultry have been exposed to H5N1 viruses.Methods We collected sera from healthy poultry-collecting-facility (PCF) workers in Jakarta and healthy poultry-farmers in Sukabumi which have close contact with poultry. Anti-H5N1 antibodies were tested with modified Haemagglutination Inhibition (HI) assay using A/Ck/Banten/05-1116/05(H5N1) antigen and with Neutralization (NT) assay using A/H5N1/Indo/05/IBCDC-RG virus.Results Among the 216 PCF worker sera and the 495 poultry-farmer sera that we collected, we found that all poultry-farmers were seronegative and one percent of poultry-collecting-facilities workers were seropositive by both HI and 1% by NT assays.Conclusions This study detected asymptomatic H5N1 virus infection among poultry workers in PCFs with intensive contact with various types of different poultry who had different titers of antibody, but no antibodies were detected among poultry farmers. (Med J Indones 2010; 19:124-9)Keywords: Avian infl uenza, farmers, poultry workers, seropositive
Sebaran Geografi Non Polio Enterovirus (NPEV) dari Kasus Layu Akut di Bagian Barat Indonesia 2007-2010 Pangesti, Krisna N.A.; Susanti1,, Nike; -, Yeremiah
Jurnal Biotek Medisiana Indonesia Vol 2, No 2 (2013)
Publisher : Central Basic Biomedical and Health Technology

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Abstract

Acute Flaccid Paralysis (AFP) surveillance which comprises cases and laboratory investigation has been established by WHO in order to achieve the goal of global eradication of polio. NIHRD is one of the national reference polio laboratories in Indonesia that conduct diagnostic test for AFP case specimens from the Western part of Indonesia. AFP cases can be caused by poliovirus and non polio viruses, such as enteroviruses. This recent study investigate the serotype of Non Polio Entero Viruses (NPEV) from AFP cases in 2007-2010 and its geographical distribution. This recent study used biological archieve from AFP cases in 2007-2010. APF specimens was cultured in cell line (RD/L20B) in BSL-2 laboratory. Positive isolates from RD cell lin in negative in L20B cell line in were tested by neutralization test to determine serotype of NPEV. Demography data were also collected from case investigation form of the patients and geographical data was analyzed using GIS arc View 3.3. From a total 2525 AFP cases, 6,8% are NPEV. NPEV cases were mostly found in North Sumater province (20,4%), There is variation fo the number of NPEV cases found each year in 16 province that sen AFP specimens to NIHRD. NPEV cases is commonly found in age group 1-5 years old, (54,3% in 2007, 86,7% in 2008, 62,2% in 2009 and 66,3% in 2010). Serotyping using WHO standard antisera showed that Echovirus is the common serotype found in 2007-2010. However, there were unidentified or untypeable NPEV isolates that need further investigation. NPEV is one of the etiologies that cause Acute Flaccid Paralysis (AFP) in Western part of Indonesia. Further detection of NPEV serotype using molecular tehcnology is needed to reveal other etiologies of AFP.Key words: Acute Flaccid Paralysis, Non Polio Enterovirus, Serotype AbstrakSurveilans lumpuh layu akut atau Acute Flaccid Paralysis (AFP) yang terdiri dari investigasi kasus dan pemeriksaan laboratorium telah ditetapkan oleh WHO sebagai bagian program pemberantasan polio global. Laboratium Virologi Badan Litbangkes adalah salah satu laboratium rujukan Nasional penyakit polio di Indonesia yang melakukan tes diagnostik untuk spesimen kasus AFP dari Indonesia bagian Barat. Kasus AFP dapat disebabkan oleh virus polio dan virus non polio seperti enterovirus. Tujuan penelitian ini adalah untuk menyelidiki serotipee Non Polio Entero Virus (NPEV) dari kasus AFP pada tahun 2007-2010 dan distribusi geografisnya. Studi ini menggunakan spesimen tersimpan dari kasus AFP pada tahun 2007-2010. Spesimen AFP dikultur dalam lini sel (RD/L20B) di laboratorium BSL-2. Isolat positif dari lini sel RD dan negatif pada lini sel L20B diuji netralisasi untuk menentukan serotipe NPEV. Data denografi juga dikumpulkan dari bentuk kasus investigasi dari pasien sedangkan, data geografis dianalisis menggunakan GIS View 3.3. Dari total 2625 kasus AFP, ditemukan 6,8% kasus NPEV. Kasus NPEV terbanyak ditemukan di provinsi Sumatera Utara (20,4%). Terdapat variasi jumlah kasus NPEV yang ditemukan setiap tahunnya pada 16 provinsi yang mengirim spesimen AFP ke Laboratium Badan Litbangkes. Kasus NPEV umunya ditemukan dalam kelompok usia 1-5 tahun, (54,3% pada tahun 2007, 86,7% pada tahun 2008, 62,2% pada tahun 2009, dan 66,3% pada tahun 2010). Serotyping menggunakan antiserum standar WHO menunjukkan bahwa Echovirus adalah serotipe yang paling banyak ditemukan pada spesimen AFP yang dikumpulkan pada tahun 2007-2010. Namun, terdapat isolat NPEV tidak dikenal atau untypeable yang perlu penyelidikan lebih lanjut. Echovirus merupakan salah satu etiologi yang menyebabkan Acute Flaccid Paralysis (AFP) di Indonesia bagian Barat. Deteksi lebih lanjut serotipe NPEV menggunakan teknologi molekuler diperlukan untuk mengungkapkan etiologi lain dari AFP.Kata Kunci: Acute Flaccid Paralysis, Non Polio Enterovirus,Serotype