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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Animal Production : Indonesian Journal of Animal Production ZOOTEC
U Paputungan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Production of Progenies by Different Growth Hormone Genotypes (GH-MspI) of Their Parents Using PCR-RFLP in Ongole-crossbred Cattle Paputungan, U; Hakim, L; Ciptadi, G; Lapian, HFN
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.188 KB)

Abstract

Abstract. The objective of this study was to identify different growth hormone (GH) genotypes using MspI enzyme-restriction in parental cows and bulls mated by artificial insemination influencing body weight and average daily gain of their progenies. Total of 74 blood samples of Ongole-crossbred cows and their female progenies and 2 blood samples of bulls of Ongole breed were used in this study. Blood samples were screened for the presence of GH gene using PCR-RFLP method involving Msp1enzyme-restriction on 1.2 % agarose gel. To eliminate different age effects of the progenies, body weight data were adjusted for the 50 and 345days old of ages for the first and second weighing, respectively. Data were analyzed using statistical program in Excel XP. The results showed that various genetic factors of growth hormone Msp1 restricted enzyme had significant influence on growth performance and average daily gain of Ongole-crossbred progenies during 50 to 345 days of age. The heterozygous genotypes of the growth hormone Msp1+/- restricted enzyme excelled over their homozygous genotypes in respects of body weight gain. Therefore, the Msp1+/+, Msp1+/- and Msp1-/- genotypes can be used as the candidate genes in Ongole crossbred cattle to improve their body weight.Keywords: Ongole-crossbred cattle, body weight gain, growth hormone, Msp1 gene. Abstrak. Tujuan dari penelitian ini adalah untuk mengidentifikasi genotipe hormon pertumbuhan yang berbeda (GH) menggunakan pembatasan enzim MspI pada sapi induk dan pejantan yang dikawinkan melalui inseminasi buatan yang mempengaruhi bobot badan dan rata-rata pertambahan bobot badan harian keturunannya. Total 74 sampel darah sapi betina persilangan Ongole dan keturunan betinanya serta 2 sampel darah sapi pejantan bangsa Ongole digunakan dalam penelitian ini. Sampel darah diperiksa untuk kehadiran gen GH menggunakan metode PCR-RFLP yang melibatkan pembatasan enzim Msp1 pada gel agarosa 1,2 %. Untuk menghilangkan efek umur yang berbeda dari keturunan, data bobot badan disesuaikan ke arah 50 dan 345 hari untuk masing-masing penimbangan pertama dan kedua. Data dianalisis dengan menggunakan program statistik pada Excel XP. Hasil penelitian menunjukkan bahwa berbagai faktor genetik hormon pertumbuhan enzim terbatas Msp1 memiliki pengaruh yang signifikan terhadap kinerja pertumbuhan dan rata-rata pertambahan bobot badan harian keturunan persilangan Ongole pada umur 50-345 hari. Genotipe heterozigot dari hormon pertumbuhan enzim terbatas Msp1 + / - mengungguli genotipe homozigot mereka dalam hal penambahan bobot badan tubuh. Oleh karena itu, genotipe Msp1 + / + , Msp1 + / - dan Msp1 - / - dapat digunakan sebagai gen bakal pada sapi persilangan Ongole untuk meningkatkan bobot badan mereka. Kata kunci: Sapi persilangan Ongole, pertambahan bobot badan, hormon pertumbuhan, gen Msp1.U Paputungan et al./Animal Production 15(1):53-61, January 2013
RIPITABILITAS KECEPATAN LARI DAN TINGGI PUNDAK ANAK KUDA PACU UMUR BERBEDA PADA KETURUNAN PEJANTAN “MANGUNI MAKASIOW” DENGAN METODE KORELASI DALAM KLAS (Intraclass corelation) Ambo, Sitty Fatimah S.; Adiani, S.; Paputungan, U; Paat, J.
ZOOTEC Vol 34, No 2 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (583.138 KB) | DOI: 10.35792/zot.34.2.2014.5529

Abstract

SPEED AND BODY HEIGHT REPEATABILITIES OF RACING HORSE AT DIFFERENT AGE IN OFFSPRING FROM “MANGUNI MAKASIOUW” MALE PARENT WITH INTRACLASS CORRELATION . This study was conducted to determine the repeatability values of speed and body height at different ages of the offspring from “Manguni Makasiouw” male parent using intraclass correlation method. Data used in this study were the records of speed to run with various distance of race horse resulting from racehorse competition in North Sulawesi and national PORDASI competition. Running speed data were tabulated using the method of analysis of variance process to obtain the values of repeatabilities. Based on the research and discussion, running speed of racehorse was the highest in the age of five years old and the lowest in the age of two years old. The repeatability values in this study were relatively low, ranging from 0.029-0.047 (running speed) and from 0.029-0.05 (body height). The value of the correlation between running speed and body height at age 2-5 years had high correlation values ​​(0.760 to 0.961), indicating that the higher the body height, the higher the level of horse running speed. Keywords: Racing Horses, repeatability, speed, body height, different Ages.
UPAYA PENGEMBANGAN POPULASI TERNAK BABI MELALUI TEKNIK INSEMINASI BUATAN DIPROVINSI SULAWESI UTARA Mandey, Freelce J; Paputungan, U; Pudjihastuti, E
ZOOTEC Vol 38, No 1 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.557 KB) | DOI: 10.35792/zot.38.1.2018.18661

Abstract

ABSTRACTEXPEDIENCY OF INCREASING PIG POPULATION BY ARTIFICIAL INSEMINATION TECHNIQUE IN NORTH SULAWESI PROVINCE.The objective of this study was to evaluate dominant factors affecting pig population breeding by artificial insemination (AI) in North Sulawesi province. Semen of boar (Landrace, Yorkshire and Duroc breeds) were usedto mate the same breeds using AI.Data of 345 piglets from 33 sows were involved in this study. Reproductive data of sows and productivity of piglet form AI were observed at laboratory and field belonging to farmers in Minahasa regency. Pair data of male and female effect were analyzed by t-test for variables.Dominant reproduction factors affecting pig population breeding in North Sulawesi were includingservice per conception, Farrowing rate, AI service efficiency, and Farrowing index.Group of male and superior piglets were higher in productivity that those of female and inferior piglets. Preweaning Average Daily Gain (PW-ADG)groups of inferior (84.85g/h/d)were significantly lower compared with normal (86.59g/h/d), butnot significantly different with PW-ADG of superior birth weight group (85.64 g/h/d).PW-ADG.Thelitter size produced from semen bya boar naturally mated with the same sowsreached the average of 21 piglets per year, while those by a boar mated using AI technique with the same sowsreached the average of 225 piglets per year. Keywords:Artificial insemination. Pig reproduction, North Sulawesi province 
PENAMPILAN REPRODUKSI KUDA BETINA PASCA PACU DI DESA PINABETENGAN RAYA KECAMATAN TOMPASO BARAT KABUPATEN MINAHASA Moningka, F A; Lapian, H; Paputungan, U; Turangan, S
ZOOTEC Vol 36, No 2 (2016)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.405 KB) | DOI: 10.35792/zot.36.2.2016.12785

Abstract

ABSTRACT   REPRODUCTION PERFORMANCE OF FORMER RACING FEMALE HORSE AT PINABETENGAN VILLAGE WEST TOMPASO DISTRICT OF MINAHASA REGENCY. Horse was the special animal to gain more money compare with any other animals. Breeding management related to reproduction of racing female horse must be taken intensively to produce a standard racing Indonesian horse called Indonesian racing horse (Kuda Pacu Indonesia, KPI). This study was conducted to evaluate reproduction performance of former racing female horse including first mating age, period time of estrus cycle, service per conception, foaling interval at Pinabetengan village, west Tompaso district of Minahasa regency. This village was a core area of farmer maintaining more racing horse in Minahasa regency. This study was done using purposive sampling method and focused on 103 former racing female horses as case study observation in obtaining primer data. The interview was also conducted to the owners of racing horse. Results showed that the averages of first mating age was 5.72 years, period time of estrus cycle was 21.67 days, service per conception was 1.65, and foaling interval was 11.98 months. Therefore, it can be concluded that former racing female horses at Pinabetengan village were included at good category of reproduction performance.   Key words: First mating age, estrus cycle period time, service per conception, foaling interval, Pinabetengan village  
Live Weight Estimation by Chest Girth, Body Length and Body Volume Formula in Minahasa Local Horse B J Takaendengan; U Paputungan; R R Noor; S Adiani
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.441 KB) | DOI: 10.5398/medpet.2012.35.2.80

Abstract

Study was conducted in the regency of Minahasa to estimate horse live weight using its chest girth, body length and body volume formula (cylinder volume formula) represented by animal chest girth and body length dimensions, particularly focused in Minahasa local horses. Data on animal live weight (LW), body length (BL), chest girth (CG) and body volume were collected from 221 stallions kept by traditional household farmers. Animal body volume was calculated using cylinder volume formula with CG and BL as the components of its formula. Regression analysis was carried out for LW with all the linear body measurements. The data were classified on the basis of age. Age significantly (P < 0.05) influenced the body measurements except animal body length (P>0.05). Animal live weight was predicted by simple regression models using dependent variable (Y) of the animal live weight and independent variable (X) of the animal body measurement, either body length, chest girth, or body volume. The correlations between all pairs of measurements were highly significant (P < 0.01) for all age groups. Regression analysis showed that live weight could be predicted accurately from body volume (R2= 0.92) and chest girth (R2= 0.90). Simple regression model that can be recommended to predict horse live weight based on body volume with their age groups ranging from 3 to ≥10 years old was as follow: Live weight (kg)= 5.044 + 1.87088 body volume (liters). The analyses of data on horse chest girth, body length and body volume formula provided quantitative measure of body size and shape that were desirable, as they enable genetic parameters for these traits to be estimated and also included in breeding programs.
Co-Authors B J Takaendengan E Pudjihastuti F A Moningka, F A G Ciptadi H Lapian, H HFN Lapian, HFN J. Paat L Hakim Mandey, Freelce J R R Noor S Adiani S Turangan, S S. Adiani Sitty Fatimah S. Ambo