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All Journal Jurnal Penelitian Kelapa Sawit
Dian Rahma Pratiwi
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Agriculture, Biological Sciences & Forestry

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Pengaruh Waktu Paparan Zat Pengatur Tumbuh Terhadap Tingkat Abnormalitas Klon Kelapa Sawit Dian Rahma Pratiwi; Sri Wening; Erwin Nazri
Jurnal Penelitian Kelapa Sawit Vol 28 No 1 (2020): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1036.007 KB) | DOI: 10.22302/iopri.jur.jpks.v28i1.96

Abstract

One of components frequently found in plant culture media is plant growth regulator (PGR), which is needed to stimulate plant culture growth. Excessive PGR addition can induce clone abnormality. It is needed comprehensive study to understand the effect of time exposure of PGR to oil palm clone abnormality in the field. This research aims to study the effect of total exposure time of PGR as well as exposure time of each medium containing different PGRs to abnormality rate of oil palm clones in the field. Four lines of oil palm clones with aged 4 years (P,Q, R, and S) were used for tracking the incubation time in the laboratory and abnormality rate in the field. The relation between variables was analyzed by using correlation, multiple linear regression and simple linear regression analysis. The results showed that length of PGRs exposure time significantly affected the clone abnormality in the field. Longer the exposure time (more than 171 days), higher the abnormality rate. From the three media containing PGR, medium containing 2,4-D and 2,4,5-TCPP significantly affected and had high significant positive correlation to clone abnormality than medium containing 2,4-D and BAP. Medium containing NAA did not show any correlation with clone abnormality rate. On the contrary, the results showed that length of incubation time in media without any PGR did not affect rate of abnormality significantly and it also had negative correlation. Linear model can be used to estimate the clone abnormality rate in the field, based on exposure time of PGR during the in vitro process.
Penggunaan Alkohol dan Sodium Hipoklorit sebagai Sterilan Tunggal untuk Sterilisasi Eksplan Kelapa Sawit Dian Rahma Pratiwi; Sri Wening; Erwin Nazri; Yurna Yenni
Jurnal Penelitian Kelapa Sawit Vol 29 No 1 (2021): Jurnal Peneltian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v29i1.120

Abstract

Oil palm micropropagation through tissue culture is initiated with explants sterilization. Sterilization is a crucial stage because it determines the production rate of sterile plant cultures. Concentration and exposure time of sterilizing agents must be determined empirically to gain effective method which produces explants with low mortality. This research aimed to obtain effective protocols for unopened-leaves sterilization of oil palm using single sterilizing agents. Alcohol and sodium hypochlorite (NaOCl) at certain concentrations and duration of exposure were used as sterilization treatments. Treatments of alcohol did not show any significant differences on contamination, browning, and growth response rate based on analysis of variance (ANOVA) as well as sodium hypochlorite. The best results were shown by 70% alcohol for 5 minutes and 10% NaOCl for ten minutes. These treatments were sufficiently effective in reducing contamination with the lowest percentage of browning explants and high growth response rate. Application with higher concentration of alcohol (80% and 90%) caused death of explants, while higher concentration of sodium hypochlorite increased browning explants. The type of contamination found in culture were bacteria and fungi. Domination of bacteria was found in alcohol treatment while fungi contamination dominated in NaOCl treatment.
Co-Authors Erwin Nazri Erwin Nazri Sri Wening Sri Wening Yurna Yenni