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All Journal Jurnal Penelitian Kelapa Sawit
Erwin Nazri
Pusat Penelitian Kelapa Sawit
Agriculture, Biological Sciences & Forestry

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Sidik Jari DNA Material Kultur Jaringan Menggunakan SSR dan AFLP Sri Wening; Dian Rahma Pratiwi; Erwin Nazri; Ernayunita Ernayunita; Hernawan Yuli Rahmadi
Jurnal Penelitian Kelapa Sawit Vol 28 No 2 (2020): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v28i2.109

Abstract

Kultur jaringan dimanfaatkan sebagai alat dalam program pemuliaan dan perbanyakan material komersial kelapa sawit. Untuk mengontrol proses kultur jaringan di laboratorium, analisis DNA dapat dilakukan dalam usaha menjamin kebenaran informasi identitas serta untuk mengetahui kestabilan genetik material pada tiap tahap proses kultur jaringan. Penelitian ini dilakukan untuk mengkaji ekstraksi DNA material kultur jaringan kelapa sawit serta sidik jari DNA pada material kultur pada tiap tahapan proses kultur jaringan, menggunakan 11 marka SSR dan 6 kombinasi primer selektif AFLP. Hasil menunjukkan bahwa protokol ekstraksi DNA yang dilakukan dapat digunakan untuk memperoleh DNA dengan kuantitas dan kualitas yang cukup untuk PCR-SSR dan PCR-AFLP. Profil SSR yang sama ditunjukkan pada semua cuplikan material yang dianalisis pada tiap tahap proses kultur jaringan. Terdapat variasi hasil sidik jari DNA menggunakan AFLP, dimana terdapat profil AFLP yang berbeda pada material yang sama pada tahap kalus dan eksplan, serta embrio dan ramet. Perbedaan profil DNA pada material yang sama pada tahap kultur yang berbeda tersebut menunjukkan adanya perubahan genetik material kultur yang mungkin disebabkan oleh pengaruh proses kultur jaringan. SSR dapat digunakan untuk identifikasi atau verifikasi identitas material kultur, sedangkan marka DNA yang menunjukkan ketidakstabilan genetik material kultur dapat digunakan untuk kajian lebih lanjut mengenai perubahan genetik material kultur dalam kaitannya dengan abnormalitas klon.
Penggunaan Alkohol dan Sodium Hipoklorit sebagai Sterilan Tunggal untuk Sterilisasi Eksplan Kelapa Sawit Dian Rahma Pratiwi; Sri Wening; Erwin Nazri; Yurna Yenni
Jurnal Penelitian Kelapa Sawit Vol 29 No 1 (2021): Jurnal Peneltian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v29i1.120

Abstract

Oil palm micropropagation through tissue culture is initiated with explants sterilization. Sterilization is a crucial stage because it determines the production rate of sterile plant cultures. Concentration and exposure time of sterilizing agents must be determined empirically to gain effective method which produces explants with low mortality. This research aimed to obtain effective protocols for unopened-leaves sterilization of oil palm using single sterilizing agents. Alcohol and sodium hypochlorite (NaOCl) at certain concentrations and duration of exposure were used as sterilization treatments. Treatments of alcohol did not show any significant differences on contamination, browning, and growth response rate based on analysis of variance (ANOVA) as well as sodium hypochlorite. The best results were shown by 70% alcohol for 5 minutes and 10% NaOCl for ten minutes. These treatments were sufficiently effective in reducing contamination with the lowest percentage of browning explants and high growth response rate. Application with higher concentration of alcohol (80% and 90%) caused death of explants, while higher concentration of sodium hypochlorite increased browning explants. The type of contamination found in culture were bacteria and fungi. Domination of bacteria was found in alcohol treatment while fungi contamination dominated in NaOCl treatment.
Peran Sumber Ortet, Nomor Daun dan Waktu Inkubasi Terhadap Hasil Kalus Kelapa Sawit (Elaeis guineensis Jacq.) Ernayunita Ernayunita; Erwin Nazri; Retno Diah Setiowati; Hernawan Yuli Rahmadi; Yenni Yenni
Jurnal Penelitian Kelapa Sawit Vol 30 No 3 (2022): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v30i3.186

Abstract

Callus production is an important key stage to reproduce oil palm through tissue culture in determining the number of produced clones. Callus production rate were affected by culture medium, organ, ortet genotypes and incubation time in the culture room. Leaf of four different individuals from two genotype: DS29D x LM2T dan BJ126D x LM2T were used as ortet on this study and only leaf number -4, -5, -6, -7 and -8 were used as an explant. Callus production of each ortet source was observed monthly and used as a parameter to determine the performance of each ortet. The result showed that BJ126D x LM2T as an ortet source had significantly higher callus production compared to DS29D x LM2T. Meanwhile the callus production from different leaf number were not significantly different. The first 6 months of explant incubation was the best time to produce callus
Co-Authors Dian Rahma Pratiwi Dian Rahma Pratiwi Ernayunita Ernayunita Hernawan Yuli Rahmadi Retno Diah Setiowati Sri Wening Yenni Yenni Yurna Yenni