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All Journal METAMORFOSA Journal of Biological Sciences Natural B Majalah Obat Tradisional JIMR - Journal of Islamic Medicine Research Community Development Journal: Jurnal Pengabdian Masyarakat JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Inovasi Penelitian Berkala Penelitian Hayati Pharmademica: Jurnal Kefarmasian dan Gizi Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Journal Community Service Consortium Hexagon
Sentot Joko Raharjo
Akademi Analis Farmasi Dan Makanan Putra Indonesia Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Community Empowerment Through 2nd Generation Tempeh Innovation Towards a Food Organized Village Raharjo, Sentot Joko; Fidyasari, Ambar
JSMARTech: Journal of Smart Bioprospecting and Technology Vol 2, No 1 (2020)
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2020.002.01.43

Abstract

Tempeh is one of Indonesia's traditional foods. Currently proposed to be a world cultural heritage in UNESCO. Tempeh cannot last long, so it takes innovation that can increase the economic value of tempeh itself. The main objective of this empowerment program is to optimize the potential of “UKM” (Small and Medium Industries) in the food sector for tempeh craftsmen and reduce the losses of tempeh craftsmen for those whose tempeh is not sold. The target partner of this community assistance program is the “UKM” group Tempeh Village in Beji Village, Junrejo, Batu City who has been economically productive as Tempeh craftsmen. The method used is Asset Based Communities Development (ABCD), where the community service focuses on assets owned by the community as the main basis for program development. The efforts carried out are assistance to SMEs to deliver material on the nutritional value of 2nd generation tempeh, workshops and 2nd generation tempe making practices, entrepreneur assistance and evaluation. This innovation is expected to be a solution to the unsold tempeh problem. This assistance was carried out for 3 months. The output of this empowerment activity is in the form of material modules, 2nd generation tempeh products, understanding of the nutritional value concept of second generation tempeh and digital marketing media plans.
Mekanisme Kerja Antibiotik Ceftriaxone, Clarithromycin, dan Senyawa Aktif Quercetin pada Streptococcus pneumoniae dengan Metoda Docking In Silico Irma Damayanti; Yan Ramona; Sentot Joko Raharjo
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p01

Abstract

Pneumolysin is a protein that plays an important role in cell death and bacterial colonization of Streptococcus pneumoniae. Ceftriaxone, clarithromycin, dan quercetin has been proven (in vivo and in vitro) to prevent the action of pneumolysin. The purpose of this study was to identify interactions between ceftriaxone, clarithromycin, and quercetin in inhibiting the pneumolysin activity through in silico. The 3D structure of pneumolysin was downloaded via RCSB PDB (PDB ID:PQQA), while 3D structure of ceftriaxone (ID:5479530), clarithromycin (ID:84029), and quercetin (ID:5280343) were downloaded via PubChem. Protein preparations were done with Discovery Studio, while ligands were prepared with OpenBabel. The docking process used HEX 8.0.0 and visualization analysis with Discovery Studio. Pneumolysin-ceftriaxone interaction produces 4 hydrogen bonds, 5 hydrophobic bonds, 1 electrostatic bond, and 2 unfavorable residue bonds with a bond energy value -329.98kJ/mol. Pneumolysin-clarithromycin interaction produces 3 hydrogen bonds, 1 electrostatic bond, and 3 unfavorable residue bonds with a bond energy value -311.12kJ/mol. Pneumolysin-quercetin interaction produces 2 hydrogen bonds, 4 hydrophobic bonds, and 2 electrostatic bonds with a bond energy value -246.52kJ/mol. Keyword: pneumolysin, ceftriaxone, clarithromycin, quercetin, in silico.
AKTIVITAS SITOTOKSIK HASIL PARTISI ETIL ASETAT EKSTRAK PETROLEUM ETER DAN EKSTRAK METANOL DAUN KAYU APU (Pistiae Folium) Sentot Joko Raharjo; Rahayu Wahyu Ningsih
Majalah Obat Tradisional Vol 20, No 3 (2015)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (814.252 KB) | DOI: 10.22146/tradmedj.8858

Abstract

This study aims to explore the cytotoxic test (LC50) towards Artemia salina L. larvae of the partition ethyl acetate fractions from methanol extracts and petroleum ether (PE) extracts of Pistiae folium. The leaves of Pistiae folium was extracted using solvents soxhletation with methanol solvent and petroleum ether solvent. Both extracts successively partitioned with n-hexane and ethyl acetate, respectively. Identification of secondary metabolites was using phytochemical screening method and TLC (Thin Layer Chromatography). TLC analysis was using the eluent chloroform: methanol 3: 1 by citroboric acid spot analysis. The cytotoxic activity was using Brine Shrimp Lethality Test (BSLT) method. The result of partition from methanol extracts was 6.25% and from PE extracts was 0.69%. The phytochemical screening test showed that it contained flavonoids, saponins and steroids. The TLC analysis identified flavonoid compounds. The cytotoxic activity (LC50) of the ethyl acetate partition from methanol extracts and petroleum ether extracts of Pistiae folium were 79.9298mg/mL and 51.7608mg/mL, respectively. The result showed that the cytotoxic activity of ethyl acetate fractions partitined from methanol extract of Pistiae folium was higher than the PE extracts.
Patchouli Oil Characteristics by Optimization Result of Distillation Time of Patchouli Leaf Dewaxing and Fermentation Sentot Joko Raharjo; Rurini Retnowati; Soebiantoro Soebiantoro
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.669 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.5

Abstract

The characteristic of patchouli oil of dewaxing, fermentation and time distillation toward patchouli leaves used GC-MS have been done.   The aim of the study was to characterization of patchouli oil on distillation time of patchouli leaves of dewaxing and fermentation.  The characteristics of patchouli oil on the distillation time for 12 hours, then the distillate collected every 2 hours showed that of the best result was the 3rd fraction collected distillate time of 12 hours with a yield of 0.56 %, light yellow color, specific gravity 0.9685 g/ mL and a refractive index of 1.5095 and patchouli alcohol of 69.56 %. Characteristics of patchouli oil on distillation time (2, 4, 6, 8, 10, 12) hours showed that of the best result was time distillation for 12 hours with a yield of 6.61 %, light yellow color, specific gravity of 0.9672 g/ mL, refractive index of 1.5082 and patchouli alcohol of 45.69 %. The other components of patchouli oil detected were alpha-gurjunene, cis-thujosene, beta-patchoulene, alpha-patchoulene, beta-caryophyllene, alpha-guaiene, seychellen, aromadendrene, beta-gurjunene, alpha-humulene, alpha-bulnesene, gemacrene-D, dehidroaromadendrene, gemacrene-A, gamma-patchoulene, valencene, viridiflorene, selina-3,7-(11)-dien, nor-patchoulenol, pogostol, illudol, globulol, beta-caryophyllen oksida, viridiflorol and ledol. Patchouli oil quality to meet requirements SNI 06-2385-2006 and ISO 3757:2002.
Water Soluble Polysaccharide Yellow Bentul (Colocasia Esculenta Schott [L]) As a Candidate for Antidiabetic Agent Sentot Joko Raharjo; Mahisa Ayyuna Nailufar; Ambar Fidyasari; Erik Widarto
JIMR - Journal of Islamic Medicine Research Vol 1, No 2 (2017)
Publisher : Published by Faculty of Medicine Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.772 KB)

Abstract

ABSTRACTIntroduction: Yellow bentul tuber is one of tuber plant species which have bioactive compound of Water Soluble Polysaccharide (WSP) and potentially healthy nutrition in the therapy of metabolic syndrome disease. The purpose of this research is to prove the ability of WSP isolate to reduce blood glucose level in white mice. Methods: The yield determination of yellow bentul tube flour, yield of WSP isolates yellow bentul tuber using enzymatic method, WSP identification using HPLC with Aminex HPX-87C BIORAD5 columns, and antidiabetic activity test using white mices. Test activity was performed in six treatment groups (Normal, Induction STZ 20 mg/ kgBW, Induction STZ 20 mg / kgBW + metformin 195mg / KgBW, three treatment with STZ induction 20 mg/ kgBW and WSP isolate with concentration 200, 400, and 600mg / kgBB). Determination of blood glucose levels using glucometer and supported by observation of histologic improvement of beta pancreatic cells in white mice that have necrosis. Result: The yield of yellow bentul flour was 14%. Yield WSP isolates of yellow bentul was 4.18%. WSP levels obtained 96.91%. Blood glucose level induced by STZ 20mg/ kgBW mice decreased glucose level at 400mg/ kgBW dose in the second week and histologic improvement of beta pancreatic cells that has the most optimal necrosis at a WSP dose of 200 mg/ kgBW. Conclusion: Isolate of Water Soluble Polysaccharide of yellow bentul potentially lower blood sugar level.Kata Kunci:, Water-soluble Polysaccharides, yellow bentul tuber, antidiabetic
Potensi Antioksidan Ekstrak Kombinasi Air-Etanol Pada Simplisia Selada Air (Nasturtium officinale R. Br) Karina Tria Amanda; Sentot Joko Raharjo
PHARMADEMICA : Jurnal Kefarmasian dan Gizi Vol 1 No 2 (2022): Oktober - Maret
Publisher : LPPMKI - AKAFARMA AKFAR PIM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.959 KB) | DOI: 10.54445/pharmademica.v1i2.13

Abstract

Empirically, selada air has a potency as a vegetable rich in antioxidants, but there has been no study of the antioxidant activity potency of selada air simplisia maceration with water-ethanol combination solvent. This study aims to determine the optimization of yield and antioxidant activity (IC50 value) of selada air simplisia maceration using ethanol-water combination solvent. The method of this research is the determination of the yield and IC50 value using the DPPH method from the maceration of selada air using a water-ethanol combination solvent (96, 70, 30 and 0%). The yields of selada air simplisia maceration with water-ethanol combination solvent (96, 70, 30 and 0%) are 9.25±1.62%, 15.63±1.25%, 23.63±2.38% and 19.99±3.82%. The IC50 values ​​are 613.77±1.90 ppm, 190.892±5.41 ppm, 350.78±1.77 ppm, and 2418.59±137.80 ppm. In conclusion, the optimal yield of maceration result is shown by the water-ethanol combination solvent of 30% (23.63%) and the optimal IC50 value is shown by the water-ethanol combination solvent of 70% (190.892 ppm).
EDUKASI PENERAPAN PROGRAM “GOOD MANUFACTURING PRACTICES” (GMP) DAN KEAMANAN PANGAN UNTUK PENGEMBANGAN KAMPUNG TEMPE Ambar Fidyasari; Sentot Joko Raharjo
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 1 No. 3 (2020): Volume 1 Nomor 3 Tahun 2020
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v1i3.972

Abstract

Tempe merupakan salah satu produk pangan nabati yang memiliki nilai gizi cukup tinggi. Di desa Beji kota Batu merupakan salah satu tempat pusat pengrajin tempe. Tempe yang dihasilkan merupakan tempe yang khas daerah tersebut, namun kendala yang dihadapi pengrajin belum menerapkan pentingnya Good Manufacturing Practices (GMP) dan keamanan pangan sehingga tempe yang dihasilkan kurang konstan.Adanya potensi kampung tempe tersebut, maka dilakukan program edukasi kepada para pengrajin tempe. Tujuan utama dalam program pendampingan ini adalah meningkatkan pemahaman dan pengetahuan kepada pengrajin tempe akan pentingnya penerapan Good Manufacturing Practices (GMP) dan keamanan pangan bagi produk agar dapat memperluas pasar usahanya. Adapun Mitra sasaran program pendampingan masyarakat ini adalah kelompok Perajin tempe di Kampung Tempe, Desa Beji Junrejo Kota Batu yang telah produktif secara ekonomi sebagai pengrajin Tempe. Adapun usaha yang akan dilakukan adalah edukasi kepada pengrajin tempe tentang kondisi sanitasi lingkungan, sarana dan prasarana produksi tempe serta hubungannya dengan proses produksi dan bahan baku yang digunakan apakah telah memenuhi syarat. Edukasi ini diharapkan adanya perubahan perilaku pengrajin dalam proses produksi. Metode yang digunakan adalah Participatory Action Research (PAR) berupa penyuluhan, demonstrasi serta pendampingan. Pendampingan dilakukan selama 3 bulan. Hasil pendampingan menunjukkan bahwa 95,00% responden setuju bahwa kegiatan pengabdian masyarakat bermanfaat, 89,00% responden setuju kegiatan edukasi ini sangat meningkatkan kualitas, 97.00% responden menyatakan ilmu bertambah setelah mengikuti pelatihan, dan 85.00% responden menyatakan bahwa keseluruhan kegiatan pelatihan sudah baik.
PATCHOULI ALKOHOL ISOMERS POGOSTEMON HERBA PREDICTED VIRTUALLY Sentot Joko Raharjo; Chanif Mahdi; Nurdiana Nurdiana; Wolfgang Nellen; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (565.727 KB) | DOI: 10.23869/142

Abstract

The aim of our research is predicting the alpha-patchouli alcohol isomer Pogostemon Herba as inhibitors cyclooxygenase (COX-1 and COX-2) isoenzymes. The data for the alpha-patchouli alcohol isomer (CD521903, CD442384, and/or CD6432585) Pogostemon Herba were explored from the pubchem database. Molecular interaction studies with COX-1 and COX-2 from mouse were done using the molecular docking tools Hex 6.12 and LeadIT2 Bisolve. The analysis of the alpha-patchouli alcohol compounds of patchouli oil showed that alpha-Patchouli alcohol (CD521903) binds to COX-1 at active sites including: LEU223B, ASP228B, LEU237B, ARG 332B, TRP 138A, GLU 139A, SER 142A, ASN 143A, and the interaction to COX-2 at active site including: GLN 289B, GLU 290B, ARG 222B, LYS 211B, THR 212B, HIS 214B, ASN 382B, HEM682B, GLN 454B, HIS 386B, TRP 387B, HIS 388B, VAL 274B, GLN 203B, VAL 291B, VAL 295B. The interaction hydrogen bond energy between alpha-patchouli alcohol: (CD521903-COX-1 complexes (-4 kJ/mol) and CD521903-COX-2 complexes (-8 kJ/ mol) by LeadIT2 Biosolve. This suggests alpha-patchouli alcohol CD521903 as candidate for a selective COX-2 inhibitor. These in silico data need further analyses of biological function activity.
Edukasi dan Pendampingan Pengelola Sayur Organik Baron Agro Lesanpuro Kota Malang Sentot Joko Raharjo; Ernanin Dyah Wijayanti
TRI DHARMA MANDIRI: Diseminasi dan Hilirisasi Riset kepada Masyarakat (Jurnal Pengabdian kepada Masyarakat) Vol 1, No 1 (2021)
Publisher : JTRIDHARMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.98 KB) | DOI: 10.21776/ub.jtridharma.2021.001.01.17

Abstract

Lesanpuro merupakan salah satu wilayah yang memiliki potensi dan daya tarik tersendiri karena memiliki hasil alam yang cukup dan luas lahan pertanian. Hal tersebut membuat wilayah tersebut menjadi penyumbang potensi yang berpengaruh bagi komoditas pertanian kota Malang, khususnya sayur organik dan buah yang sudah sangat jarang sekali ditemui lahan pertanian di tengah kota, namun kelompok usaha ini belum memahami benar arti pentingnya cara produksi yang baik dan keamanan produk pangan sayur organik. Edukasi dan pendampingan kelompok usaha pengolahan sayur organik yang benar dan pengetahuan meliputi semua aspek penanganan bisnis tersebut. Tujuan pengabdian masyarakat ini adalah edukasi dan pendampingan kelompok usaha dalam pengelolaan sayur organik yang baik agar menjadi produk pangan yang aman sehingga menjadi peluang usaha baru untuk meningkatkan kesejahteraan dan kemandirian dalam lingkungan, kesehatan, dan perekonomian. Metode yang digunakan adalah PAR (Participatory Action Research) berupa penyuluhan, pelatihan, demonstrasi, serta pendampingan. Pendampingan dilakukan selama 3 bulan. Hasil pendampingan menunjukkan bahwa 95% responden setuju bahwa kegiatan pengabdian kepada masyarakat bermanfaat, 89% responden setuju kegiatan edukasi ini sangat meningkatkan kualitas, 97% responden menyatakan ilmu bertambah setelah mengikuti pelatihan, dan 85% responden menyatakan bahwa keseluruhan kegiatan pelatihan sudah baik. Kesimpulan pengabdian adalah kegiatan edukasi dan pendampingan kelompok usaha dalam pengelolaan sayur organik agar menjadi produk pangan yang aman mendapatkan respons yang baik dan menjadi peluang usaha baru untuk meningkatkan kesejahteraan dan kemandirian dalam lingkungan, kesehatan, dan perekonomian.
UJI TOKSISITAS AKUT MINUMAN PROBIOTIK SIRSAK GUNUNG (Annona montana Macf.) DENGAN METODE BSLT (Brine Shrimp Lethality Test) Ambar Fidyasari; Sentot Joko Raharjo; Melani Setyowati
Jurnal Inovasi Penelitian Vol 1 No 5: Oktober 2020
Publisher : Sekolah Tinggi Pariwisata Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47492/jip.v1i5.174

Abstract

Soursop fruit (Annona montana Macf.) is one of the plants can be used as as traditional medicine. This plant contains terpenoid and acetogenin which can cause toxicity. The fruit has a flavor that is tasteless so the innovation becomes probiotic drinks. This drink has been proven as an antioxidant, antibacterial, antihyperuricemia and antidiarrheal. The aim of this study was to know about acute toxicity of probiotic drink of soursop juice using brine shrimp lethality test method which will be indicated by LC50 value. This study used experimental methods conducted in the Laboratory of Farmakoknosi. There are several variations in concentration in this study, namely 10000 ppm, 20000 ppm, 30000 ppm, 40000 ppm, 50000 ppm, 60000 ppm, 70000 ppm, 80000 ppm and replication was done 3 times with total number of test animals used was 270. The results showed that probiotic drink of soursop juice can provide acute toxic effects on test animals with LC50 value of 29717,23 ppm. LC50 values ​​indicate that the mountain soursop probiotic drink is not potentially toxic because it has a value of >1000 ppm.
Co-Authors Ambar Fidyasari Andini Andini Andini Andini Audry Regita Ayu Fardi Chanif Mahdi Erik Widarto Ernanin Dyah Wijayanti Fadli Muhammad Fatchiyah Fatchiyah Irma Damayanti Karina Tria Amanda Kiantoro Andiek Setiawan Mahisa Ayyuna Nailufar Meiria Istiana Sari Melani Setyowati Nurdiana Nurdiana Rahayu Wahyu Ningsih Rurini Retnowati Soebiantoro Soebiantoro Wolfgang Nellen Yan Ramona