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All Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal
Febriastuti Cahyani
Faculty Of Dental Medicine, Universitas Airlangga,Surabaya,Indonesia
Dentistry

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Anti-glucan effects of propolis ethanol extract on Lactobacillus acidophillus Ira Widjiastuti; Adioro Soetojo; Febriastuti Cahyani
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 1 (2017): March 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.392 KB) | DOI: 10.20473/j.djmkg.v50.i1.p28-31

Abstract

Background: In deep dentinal caries cases, bacteria mostly found are Lactobacillus acidophilus classified as gram positive bacteria and as facultative aerobes producing glucosyltransferase (GTF) enzyme. GTF enzyme can alter sucrose into glucans. Glucan is sticky and insoluble in water. As a result, GTF enzyme can facilitate plaque formation and microorganism colonization on tooth surface. In addition, Lactobacillus acidophilus also can form acid leading to demineralization of organic and inorganic materials, resulting in dental caries. Multidrug-resistant phenomena, on the other hand, have led to the use of natural resources, one of which is propolis as an antimicrobial material and as a new anti-infective therapeutic strategy. Propolis is a resinous substances collected by worker bees (Apismellifera) from barks and leaves of plants. Propolis has a complex chemical composition and biological properties, such as antibacterial, antiviral, antifungal, anti-inflammatory, and antitumor. Purpose: This research aimed to reveal anti-glucan effects of propolis ethanol extract generated from honey bee, Apis mellifera spp on Lactobacillus acidophilus bacteria. Method: Before antiglucan test was conducted, glucan-formation test was performed on Lactobacillus acidophilus bacteria using SDSpage. Meanwhile, anti-glucan adhesion test on Lactobacillus acidophilus bacteria was carried by culturing the bacteria at 37ºC temperature in a jar with 10% CO2. Test tubes were placed at an angle of 30º for 18 hours to review the attachment of bacteria at the glass surfaces. After the incubation, the culture of bacteria was vibrated using a mixer vortex for a few minutes, and then cultured in solid MRS A media. Bacteria grown were measured by using colony counter. Result: The ethanol extract of propolis with a concentration of 1.56% was the lowest concentration inhibiting the attachment of glucan to Lactobacillus acidophilus bacteria. Conclusion: The ethanol extract of propolis with a concentration of 1.56% can be used as an anti-glucan material for Lactobacillus acidophilus bacteria.
Biocompatibility of 0,78% tannin of garciniamangostanalinnpericarp extract and 0,2% chlorhexidinegluconateagainst BHK-21 fibroblast cells culture Monika Werdiningsih; Ira Widjiastuti; Febriastuti Cahyani; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (588.872 KB) | DOI: 10.20473/cdj.v10i1.2020.36-39

Abstract

Background.Chlorhexidinegluconate is one of endodontic irrigants potential due to its antibacterial activity. Although it is an effective antibacterial agent, chlorhexidinegluconate cannotdissolve organic substances and necrotic tissue present in the root canal. In addition, same as other chemicals, chlorhexidinegluconate also cause a cytotoxic effect. Tannin extracts of mangosteenpericarp (Garciniamangostana Linn.) demonstrated various biological activities including antibacterial, antioxidant, anticancer and anti-inflammatory. Tannin extracts of mangosteenpericarp can be considered as an alternative endodontic irrigation for dental application.Purpose.The aim of this study was to compare the biocompatibility between 0,78% tannin extracts of mangosteen pericarp and 0,2% chlorhexidinegluconate to BHK-21 fibroblass cell. Method.Tannins obtained from extracts of mangosteenpericarp. Preliminary test was conducted in the biocompatibility of tannin extracts of mangosteenpericarp in various concentrations of the BHK-21 fibroblass. Tannins extracts of mangosteenpericarp was at concentrations of 0,78% less toxic than the other concentrations. Then biocompatibility of 0,78% tannin extracts of mangosteenpericarp compare with 0.2% chlorhexidinegluconate using cytotoxicity test on BHK-21 fibroblass cells with MTT method and observed by ELISA reader. Results.0,78% tannins extracts of  mangosteenpericarp showed good biocompatibility with fibroblass BHK-21 than 0,2% chlorhexidinegluconate. There was a significant differences between the results of treatment with 0,78% tannin extracts of mangosteenpericarp and 0.2% chlorhexidinegluconate. Conclusion. 0,78% tannin extracts of mangosteenpericarp has better biocompatibility than 0,2% chlorhexidinegluconate as an endodontic irrigants.
Single visit root canal treatment with post crown restoration Kun Ismiyatin; Widya Saraswati; Febriastuti Cahyani; Olivia Vivian Widjaya; Dede Ghozali Pangestu; Dea Arshila
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.57-62

Abstract

Background: Single visit endodontics has been shown to be an effective treatment modality when compared with multiple visit therapy and it does not deviate from achieving the objectives of proper biomechanical preparation, debridement, shaping, disinfection and 3 dimensional obturation of root canal system and is more beneficial to the patient and dentist provided there is careful case selection and strict follow of standard endodontic protocols. Symptomatic teeth can also be managed by single visit after controlling the abscess infection with antibiotic, provided the root canal is dry without any discharge.Purpose: This study aims to perform single visit root canals on teeth with irreversible pulpitis and restoration with post core and crown. Case(s): A 48-years-old female comes with dental complaints of upper left rear spontaneous pain at night. The tooth has had cavities since about two years ago and has never been previously treated. The patient said had not any systematic health disorder. There is a spontaneous pain that the patient feels. Case Management: From the examination that has been carried out, a clinical diagnosis of tooth 25 were symptomatic pulpitis irreversible with normal apical tissue. The restoration endodontic treatment plan was a single visit endodontic and the restoration is post core and crown. Conclusion: Symptomatic pulpitis irreversible with normal apical tissue treatment can be carried out single visit root canal treatment. Restoration of post endodontic treatment in this case need fiber post and core using composite and finally restoration using crown that made of zirconia. Zirconia has a higher level of material resistance than lithium disilicate. This is what makes zirconia the choice, especially for posterior teeth.
One Visit Endodontic with Internal Bleaching on Tooth 12 Post Trauma: A Case Report Reyz Pasenda Mulyadi; Sofi Arnesti Wahab; Febriastuti Cahyani; Widya Saraswati
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.63-67

Abstract

Introduction: Post-traumatic non-vital teeth is one of the indications for One Visit Endodontic. Trauma to the teeth may arise from conditions that cause the teeth to become necrotic so that the teeth will look darker and experience discoloration. Clinical conditions that indicate the death of the dental pulp and teeth require endodontic treatment. This situation will cause discoloration of the teeth that occur as a result of the metamorphosis of calcified pulp. Internal whitening is a treatment that can be done after the first treatment. Purpose: To provide information about discolored teeth management after trauma which causes pulp necrosis by doing root canal treatment and internal bleaching to change tooth color. Case: A 31-year-old woman came to the RSKGMP Universitas Airlangga with a referral from the orthodontics department where her teeth were darker in color and interfered with her appearance. In the previous history, the patient had an accident and trauma 10 years ago. Case Management: The first step is access opening and a good glide path. Root canal preparation was performed using the Crown Down Pressureless technique. The obturation technique used is Single Cone. The results were good and there were no symptoms during the control. Conclusion: One visit endodontic can be a treatment option for post-traumatic non-vital teeth. Internal bleaching with Walking Bleach Technique is also proven effective in restoring the original tooth color without excessive reduction of the dental crown.
Shock wave enhanced emission photoacoustic streaming (SWEEPS): An alternative to conventional irrigation in endodontic treatment Jesslyn Jordana; Jason Nicholas Tandean; Jennifer Fiona Salim; Febriastuti Cahyani; Galih Sampoerno
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.68-72

Abstract

Background: Irrigation is an essential step in root canal treatment (RCT). However, the complex structure of the root canal can limit the penetration of irrigants, causing RCT failure. Shock wave enhanced emission of photoacoustic streaming (SWEEPS) has been developed to improve root canal cleaning by increasing the penetration of irrigants. SWEEPS utilized the Er:YAG laser to generate micropulse, producing bubbles delivered in liquid, causing powerful photodynamic streaming. However, its superiority compared to conventional irrigation remains unclear. Thus, we aim to review the available evidence to assess the benefits of SWEEPS over conventional irrigation. Purpose: This study aims to review the available evidence to assess the benefits of SWEEPS over conventional irrigation. Reviewa: Article searches were conducted on Pubmed, ScienceDirect, EBSCOhost, and ProQuest using SWEEPS and root canal irrigation as keywords. Articles were included if they aim to assess the benefits of SWEEPS over conventional irrigation in removing smear layer, debris, pulp tissues, or bacteria. A total of 833 articles were initially retrieved from systemic search of literature and 6 articles following inclusion criteria were included in the review. Out of 6 articles, 4 articles suggested that SWEEPS could increase the removal of smear layer, debris, pulp tissues, and bacteria in the root canal compared to conventional irrigation. However, 2 articles found contradictory results, in which SWEEPS and conventional irrigation had similar outcomes in terms of penetration depth and could not completely eradicate biofilm. Conclusion: This review suggests that SWEEPS offers more benefits over conventional irrigation since it can improve the removal of smear layer, debris, pulp tissues, and bacteria in the root canal.
Co-Authors Adioro Soetojo Dea Arshila Dede Ghozali Pangestu Dian Agustin Wahjuningrum Galih Sampoerno Ira Widjiastuti Jason Nicholas Tandean Jennifer Fiona Salim Jesslyn Jordana Kun Ismiyatin Monika Werdiningsih Olivia Vivian Widjaya Reyz Pasenda Mulyadi Sofi Arnesti Wahab Widya Saraswati