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All Journal Jurnal Kedokteran Brawijaya Jurnal Agroekoteknologi
Sri Rianawati
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Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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Pengaruh Antosianin Ubi Ungu terhadap TNF-α, Apoptosis dan Memori Spasial Hipokampus Tikus Model Diabetes Melitus Darwatik, Darwatik; Ratnawati, Retty; Rianawati, Sri; Sarwono, Imam
Jurnal Kedokteran Brawijaya Vol 30, No. 1 (2018)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2018.030.01.3

Abstract

Hiperglikemia pada diabetes melitus menyebabkan penurunan fungsi memori spasial. Terapi yang efektif terhadap gangguan fungsi kognitif akibat hiperglikemia yang sampai saat ini masih dalam perdebatan, menjadikan pendekatan pencegahan menjadi pilihan. Ubi ungu (Ipomoea batatas L.) merupakan tanaman tradisional yang mengandung antosianin, sebagai antiinflamasi, antioksidan, berhubungan dengan peningkatan signalling neuronal otak dalam memperbaiki fungsi memori. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian antosianin ubi ungu (Ipomoea batatas L.) terhadap ekspresi TNF-α, apoptosis dan fungsi memori spasial tikus wistar/(Rattus novergicus) model diabetes melitus. Pendekatan ekperimental dilakukan dalam 5 kelompok yaitu kontrol negatif, kontrol positif, antosianin dosis 10, 20, dan 80mg/kgBB selama 6 minggu. Ekspresi TNF-α dan apoptosis diukur menggunakan imunohistokimia, fungsi memori spasial dengan Morris water maze test. Dosis Antosianin 80mg/kgBB menurunkan ekspresi TNF-α dan apoptosis secara bermakna dibandingkan kontrol positif (p=0,028, p=0,025). Dosis 10 dan 20mg/kgBB menurunkan waktu tempuh Morris water maze hari ke 54 pada semua kuadran secara bermakna dibandingkan kontrol positif (p=0,000). Pemberian antosianin dosis 80mg/kgBB menurunkan ekspresi TNF-α dan apoptosis sel hipokampus, antosianin dosis 10 dan 20mg/kgBB menurunkan waktu tempuh Morris water maze pada tikus wistar/Rattus novergicus model diabetes melitus.
DAYA REGENERASI PLBs ANGGREK DENDROBIUM VAR. JACQUELINE THOMAS x WALTER OUMAE DAN KUMALA AGRIHORTI PADA JENIS MEDIA KULTUR IN VITRO DENGAN PENAMBAHAN AIR KELAPA Sri Rianawati; Sulastri Isminingsih; Nuniek Herminta; Luqyana Ulfa Riyadi
Jurnal Agroekoteknologi Vol 13, No 2 (2021)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v13i2.13167

Abstract

Dendrobium is the epiphytic orchid and high demand because of its long-lasting freshness, color variants, shape, and high productivity. The addition of organic complex Coconut Water (CW) to the in vitro media such as MS and VW because in general organic complex compounds are sources of sugars, vitamins, growth regulators, and amino acids. The purpose of this study was to obtain the best media for plantlet regeneration from PLBs of Dendrobium orchids var. JTWO and var. Kumala Agrihorti. This study used a Completely Randomized Design consisting of two factors with five replications. The combination of  planting media with the addition of coconut water was consist of 10 levels, ½ MS+0 ml/l CW, ½ MS+75 ml/l CW, ½ MS+100 ml/l CW, ½ MS+125 ml/l CW, ½ MS+150 ml/l CW, VW+0 ml/l CW, VW+75 ml/l CW, VW+100 ml/l CW, VW+125 ml/l CW and VW+150 ml/l CW. The results showed that the variety treatment gave a significant effect on the percentage of PLBs regeneration, the number of shoots, and the increase in plantlet height. The treatment of media gave a significant effect on the percentage of PLBs regeneration, percentage of PLBs life, the number of leaves, and the number of roots. The interaction between treatments significantly affects the percentage of PLBs regeneration.
Co-Authors Darwatik, Darwatik Imam Sarwono Luqyana Ulfa Riyadi Nuniek Herminta Retty Ratnawati Sulastri Isminingsih