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All Journal Jurnal Ilmu Ternak dan Veteriner JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES
RONI RIDWAN
Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Bogor 16911Indonesian
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology Veterinary

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 1 
Utilization of Probiotic and Organic-Cn on Ruminal Ecosystem In Vitro Astuti, Wulansih Dwi; Ridwan, Roni; Tappa, Baharuddin
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 4 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.992 KB) | DOI: 10.14334/jitv.v12i4.493

Abstract

Improvement of nutrient intake was needed to increase cattle productivity. Probiotics and organic Cr, as feed additives, might have beneficially improve ruminant microbial ecosystem, and optimize digestion process by rumen microbes. The objective of this study was to examine the effects of combination between probiotic or mixed probiotcs and organic Cr on rumen fermentation activity. An in vitro study was held based on randomized block design with 5 treatments and 3 replications. The treatments were (A) Control ration, (B) Control ration + probiotic TSD 10, (C) Control ration + probiotic TSD 10 + organic Cr, (D) Control ration + mixed probiotics, and (E) Control ration + mixed probiotics + organic Cr. Organic Cr concentration was supplemented 2 ppm, whereas probiotics supplement contained 109 cfu/g. Supplementation of probiotic and organic Cr increased dry matter and organic matter digestibility but decreased total VFA and NH3 concentration. Treatment E (combination between mixed probiotics and organic Cr) gave the highest dry matter (52.45 %) and organic matter (51.96 %) digestibilities. It also tended to increase NH3 and total VFA production. Supplementation of single or mixed probiotics showed no difference for dry matter and organic matter digestibilities, and proportion of individual VFA. Mixed probiotics gave higher VFA and NH3 concentration compared to single probiotic. Probiotics supplementation resulted in a tendency for higher acetate proportion, while organic Cr supplementation resulted in a tendency of higher propionate proportion. Key Words: Probiotic, Organic Cr, Ruminal Microbes, VFA
Giving of Formulated Pellet on Javan Porcupine (Hystrix javanica F. Cuvier, 1823): Effects on Feed Intake, Feed Conversion, and Digestibility in Pre-Domestication Condition Farida, Wartika Rosa; Ridwan, Roni
JURNAL BIOLOGI INDONESIA Vol 7, No 1 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i1.3136

Abstract

ABSTRAKPemberian pelet formula pada landak Jawa (Hystrix javanica F. Cuvier, 1823) : pengaruhnyaterhadap konsumsi, konversi pakan, dan kecernaan pada kondisi pra budidaya. Penelitiantelah dilakukan untuk mengetahui pengaruh pemberian pelet formula pada landak Jawa terhadapkonsumsi, konversi pakan, dan kemampuan cerna. Penelitian berlangsung selama 40 hari (12hari preliminary dan 28 hari masa pengumpulan data) di Penangkaran Mamalia Kecil, BidangZoologi, Pusat Penelitian Biologi – LIPI, Cibinong. Selama penelitian, delapan ekor landakmasing-masing ditempatkan di dalam kandang metabolik berukuran 1,4 m x 1,2 m x 1,0 m (panjangx lebar x tinggi). Rancangan penelitian adalah rancangan acak lengkap terdiri dari 4 perlakuanransum dan dua ulangan yaitu ransum kontrol (T0), ransum diberi pelet formula 50 g/ekor/hari(T1), ransum diberi pelet formula 100 g/ekor/hari (T2), dan ransum diberi pelet formula 150 g/ekor/hari (T3). Hasil penelitian menunjukkan tingkat kesukaan landak Jawa terhadap jenispakan penelitian berturut-turut adalah bengkuang, ketimun, talas belitung, pelet formula,kangkung, jagung manis, daun kitengis, kelapa, ubi jalar, dan kunyit. Konsumsi nutrien, kecualilemak pada perlakuan pemberian pelet formula (T1, T2, T3), lebih tinggi dibanding perlakuankontrol (T0). Konversi pakan pada landak T0 lebih tinggi dibandingkan pada landak T1, T2,dan T3, masing-masing 12,45%; 8,15%; 6,98%;, dan 5,58%. Kecernaan bahan kering adalah94,44% (T0); 95,79% (T1), 95,08% (T2); dan 97,16% (T3), sedangkan kecernaan bahan organikadalah 94,55% (T0), 96,16% (T1); 95,64% (T2); dan 97,45% (T3). Total digestible nutrien(TDN) tinggi pada semua perlakuan, yaitu 90,40% (T0); 92,69 % (T1); 92,03% (T2); dan 94,25%(T3) yang menggambarkan landak mampu mencerna semua nutrien pakan dengan baik.Kata kunci: pelet formula, konsumsi, konversi pakan, kecernaan, Hystrix javanica
Diversty of Actinomycetes from Soil Samples Collected from Lombok Island, Indonesia Lisdiyanti, Puspita; Tamura, Tomohiko; Ratnakomala, Shanti; Ridwan, Roni; Kartina, Gina; Lestari, Yulin; Katsuhiko, Ando; Widyastuti, Yantyati
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.783 KB) | DOI: 10.1234/52

Abstract

A total of 137 strains of Actinomycetes were isolated from 14 soil samples collected in 3 part of Lombok Island, Indonesia  using  SDS-YE (SY) and Rehydration and Centrifugation  (RC)  isolation methods  and Humic Acic Vitamin (HV) agar as isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. On the basis of their morphology and 16S rRNA gene sequence, 67% of isolates were belonged  to the Streptomyces Group and 33% of isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Nine families and 15 genera were found from the samples. It is indicated the richness of actinomycetes in these area. The genus  Streptomyces  is the most abundant in all soil samples, occupying 67% of all isolates by using SY method; while the genusActinoplanes  is mainly found by using RC as isolation method. Two strains belong to the genus Virgisporangium and Catenulispora that showed taxonomic interest need further study for describing as ne species.   Keywords: Actinomycetes, Streptomyces Group, Non-Streptomyces Group, Diversity
Ecological Study of Rare-Actinomycetes in Soils and Leaf-Litters Lisdiyanti, Puspita; Ratnakomala, Shanti; Ridwan, Roni; Widyastuti, Yantyati; Otoguro, Misa; Katsuhiko, Ando
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.626 KB) | DOI: 10.1234/49

Abstract

A total of 268 strains of Actinomycetes were isolated from soil and leaf-litter samples collected in Jambi (Sumatera) and Cibinong (West Java) using three selective isolation methods. All the isolates were identified by morphological characteristic and by analysis of 16S rDNA sequence. On the basis of their morphology and 16S rDNA sequence, 164 isolates were belonged to the Streptomyces Group and 104 isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Furthermore, 40% of isolates in Streptomyces Group and 62% of isolates in Non-Streptomyces Group are supposed to be new taxa. It is indicated the richness of Actinomycetes in these two area. The genus Streptomyces is the most abundant in soil samples, occupying 60- 75% of all isolates; while the genus Actinoplanes is mainly found in leaf-litter samples (70%). The use of selective isolation media is important to elucidate the microbial diversity.Key words: Actinomycetes, microbial diversity
GIVING OF FORMULATED PELLET ON JAVAN PORCUPINE (HYSTRIX JAVANICA F. CUVIER, 1823): EFFECTS ON FEED INTAKE, FEED CONVERSION, AND DIGESTIBILITY IN PRE-DOMESTICATION CONDITION Farida, Wartika Rosa; Ridwan, Roni
JURNAL BIOLOGI INDONESIA Vol 7, No 1 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i1.3136

Abstract

ABSTRAKPemberian pelet formula pada landak Jawa (Hystrix javanica F. Cuvier, 1823) : pengaruhnyaterhadap konsumsi, konversi pakan, dan kecernaan pada kondisi pra budidaya. Penelitiantelah dilakukan untuk mengetahui pengaruh pemberian pelet formula pada landak Jawa terhadapkonsumsi, konversi pakan, dan kemampuan cerna. Penelitian berlangsung selama 40 hari (12hari preliminary dan 28 hari masa pengumpulan data) di Penangkaran Mamalia Kecil, BidangZoologi, Pusat Penelitian Biologi ? LIPI, Cibinong. Selama penelitian, delapan ekor landakmasing-masing ditempatkan di dalam kandang metabolik berukuran 1,4 m x 1,2 m x 1,0 m (panjangx lebar x tinggi). Rancangan penelitian adalah rancangan acak lengkap terdiri dari 4 perlakuanransum dan dua ulangan yaitu ransum kontrol (T0), ransum diberi pelet formula 50 g/ekor/hari(T1), ransum diberi pelet formula 100 g/ekor/hari (T2), dan ransum diberi pelet formula 150 g/ekor/hari (T3). Hasil penelitian menunjukkan tingkat kesukaan landak Jawa terhadap jenispakan penelitian berturut-turut adalah bengkuang, ketimun, talas belitung, pelet formula,kangkung, jagung manis, daun kitengis, kelapa, ubi jalar, dan kunyit. Konsumsi nutrien, kecualilemak pada perlakuan pemberian pelet formula (T1, T2, T3), lebih tinggi dibanding perlakuankontrol (T0). Konversi pakan pada landak T0 lebih tinggi dibandingkan pada landak T1, T2,dan T3, masing-masing 12,45%; 8,15%; 6,98%;, dan 5,58%. Kecernaan bahan kering adalah94,44% (T0); 95,79% (T1), 95,08% (T2); dan 97,16% (T3), sedangkan kecernaan bahan organikadalah 94,55% (T0), 96,16% (T1); 95,64% (T2); dan 97,45% (T3). Total digestible nutrien(TDN) tinggi pada semua perlakuan, yaitu 90,40% (T0); 92,69 % (T1); 92,03% (T2); dan 94,25%(T3) yang menggambarkan landak mampu mencerna semua nutrien pakan dengan baik.Kata kunci: pelet formula, konsumsi, konversi pakan, kecernaan, Hystrix javanica
Co-Authors ANDO KATSUHIKO Baharuddin Tappa GINA KARTINA MISA OTOGURO PUSPITA LISDIYANTI SHANTI RATNAKOMALA TOMOHIKO TAMURA Wartika Rosa Farida Wulansih Dwi Astuti Yantyati Widyastuti YULIN LESTARI