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PEMURNIAN DAN KARAKTERISASI XILANASE Streptomyces sp. SKK1-8 Anja Meryandini; Nunuk Widhyastuti; Yulin Lestari
Makara Journal of Science Vol 12, No 2 (2008): November
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Abstract

Purification and Characterization of Streptomyces sp. SKK1-8 xylanase. Streptomyces sp. SKK1-8 is a xylanase produced bacteria. Purified xylanase has an optimum condition at pH 4.5 and 50oC. The molecular mass of purified xylanase were determined to be 14.4 kDa and 13.4 kDa. The xylanase was capable of hydrolysing p-NP-α-L-arabinofuranoside, p-NP-β-D-xylanopiranoside, p-NP-β-D-glucopiranoside, p-NP-α-D-galactopiranoside. The Km and Vmax values at 50oC measured on Birchwood xylan were 0.101 mg/ml and 0.1796 μmoles/minute/ml. Keywords: Xylanase, streptomyces, purified
STUDI MIKROBIOLOGI DAN SIFAT KIMIA MIKROORGANISME LOKAL (MOL) YANG DIGUNAKAN PADA BUDIDAYA PADI METODE SRI (System of Rice Intensification) Suhastyo, Arum Asiyanti; Anas, Iswandi; Santosa, Dwi Andreas; Lestari, Yulin
SAINTEKS Vol 10, No 2 (2013): Jurnal Sainteks Volume X No 2 Oktober 2013
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH PURWOKERTO

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Abstract

Penggunaan larutan mikroorganisme lokal (MOL) dalam metode SRI budidaya padi yang dikembangkan di Indonesia dimulai awal sejak persiapan bibit fase vegetatif, pembentukan malai dan pengisian bulir padi. MOL adalah cairan yang dapat dibuat dari bahan yang tersedia di sekitar kita seperti sisa sayuran, rebung, keong mas, buah maja, daun gamal, bonggol pisang, nasi, urine kelinci, dan lain-lain. Cairan umumnya diberikan 10, 20, 30, 40 dan 60 hari setelah tanam (HST ) atau sesuai kebutuhan. Penelitian ini menggunakan larutan MOL yang terbuat dari bonggol pisang, keong mas dan urin kelinci. Tujuan penelitian adalah untuk menentukan mikrobaa, identifikasi mikrobaa dan sifat kimia dalam MOL bonggol pisang, keong mas dan urin kelinci. Penelitian ini dilakukan di Laboratorium Bioteknologi Tanah IPB. Penelitian ini menggunakan rancangan acak lengkap dengan satu faktor (waktu) dan tiga kali pengulangan. Hasil penelitian menunjukkan MOL bonggol pisang memiliki rata-rata nilai pH terendah (4,2-4,5) dan nilai EC tertinggi (10,44-12,82 mikrodetik/cm) selama proses fermentasi. MOL keong emas memiliki rata-rata pH tertinggi (4,5-6,55) dan dan yang paling oksidatif - reduktif nilai Eh [ 269-(-381) mV ] selama proses fermentasi. MOL urin kelinci memiliki nilai rata-rata terendah dari EC (2,18-2,23 mikrodetik/cm) dan mengandung lebih banyak unsur K, Ca, Mg, Cu, Zn, Fe dan Mg dari kedua jenis MOL lainnya. Selanjutnya, Bacillus sp., Aeromonas sp. dan Aspergillus niger diidentifikasi dalam MOL dari bonggol pisang. MOL dari keong mas mengandung Staphylococcus sp. dan Aspergillus niger, sedangkan MOL urin kelinci memiliki Bacillus sp. , Rhizobium sp. , Pseudomonas sp. , Aspergillus niger dan Verticillium sp.Kata kunci : SRI, MOL, sifat kimia MOL, identifikasi mikrobaa
Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten Streptomyces spp. isolates in suppressing Xoo disease was assessed in planta and in vitro. In planta experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both Streptomyces spp. and Xoo, and plants i RATIH DEWI HASTUTI; YULIN LESTARI; ANTONIUS SUWANTO; RASTI SARASWATI
HAYATI Journal of Biosciences Vol. 19 No. 4 (2012): December 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.4.155

Abstract

Xanthomonas oryzae pv. oryzae (Xoo), a causal agent of bacterial leaf blight (BLB), is one of the most important pathogens of rice. The effectiveness of ten Streptomyces spp. isolates in suppressing Xoo disease was assessed in planta and in vitro. In planta experiments were carried out in a greenhouse and arranged in a randomized completely block design (RCBD) with three replications. Twenty treatments were tested which included plants inoculated with both Streptomyces spp. and Xoo, and plants inoculated with only Streptomyces spp. Plants inoculated with Xoo and sprayed with a chemical bactericide, and plants inoculated with only Xoo served as positive controls, whereas plants not inoculated with either Streptomyces spp. or Xoo were used as negative controls. The results showed that the effect of endophytic Streptomyces spp. on BLB disease expressed as area under disease progress curve (AUDPC) was not significantly different to that on control plants (P > 0.05). However, plants inoculated with endophytic Streptomyces spp. were significantly taller and produced higher tiller number than control plants (P < 0.05). Streptomyces spp. isolate AB131-1 gave the highest plant height. In vitro studies on biocontrol mechanisms of selected Streptomyces spp. isolates showed that isolate LBR02 gave the highest inhibition activity on Xoo growth, followed by AB131-1 and AB131-2. Two isolates (AB131-1 and LBR02) were able to produce chitinase, phosphatase, and siderophore which included biocontrol characteristics. Morphological and colonization studies under SEM and light microscopy confirmed that the three isolates were endophytic Streptomyces spp. from different species. These studies found that the paddy plant which was inoculated with endophytic Streptomyces spp. AB131-1 and infected by Xoo could increase the height of plant and number of tillers.
The Existence of Endophytic Actinobacteria from Rhododendron zoelerri Revealed by Culture-Dependent and Culture-Independent Approaches Yulin Lestari; Lia Aseptin Murdini; Dedy Duryadi Solihin
HAYATI Journal of Biosciences Vol. 25 No. 2 (2018): April 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (559.98 KB) | DOI: 10.4308/hjb.25.2.54

Abstract

Endophytic actinobacteria from medicinal plant may play a significant role in producing bioactive compounds. The information regarding their diversity is an important.  Rhododendron are traditionally used for treating human disorders. One of the selected Rhododendron used in this study was R.  zoelleri from Papua origin, which has been conserved and grown in Cibodas Botanical Garden, West Java, Indonesia. The aim of this study was to assess the existence of endophytic actinobacteria from R. zoelleri based on a culture-dependent and their community structure based on a culture-independent approach. Culturable actinobacteria were isolated and cultured on HV medium. Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) targeting the metagenomic 16S rRNA was used to analyse the structure of the actinobacterial community. Six culturable endophytic actinobacteria (200 cfu/g fresh weight) from R. zoelleri were successfully isolated, three isolates from leaf, and the other isolates were obtained from stem. The six culturable isolates were RZP 1.3, RZP 1.1, RZP 2.2, RZPB 1.1, RZPB 7.1, RZPB 4.1. Based on their morphological characteristics, the endophytes have Streptomyces characters. The existence of Streptomyces spp. were also confirmed with molecular analysis based on 16S rRNA gene. The phylogenetic analysis based on 16S rRNA gene to the reference strains available in EzTaxon-e database showed that six isolates were closely related to S. djakartensis strains of NBRC 15409ᵀ(99.19%), S. tritolerans strains of DAS 165T(99.90%), S. coelicoflavus strains of NBRC 15399T(99.59). However, they showed differences in morphological characteristics as compared with the reference strains. The metagenomic analysis of the DGGE profile based on 16S rRNA gene showed the community structure of endophytic actinobacteria from R. zoelleri which was represented by 13 DGGE bands. The bands were closely related to Agromyces, Gordonia, Microbacterium, Micromonospora, Propionibacterium, Saccharomonospora, Streptomyces which have 93.18%-100% similarity. Based on the data, it showed diversity of endophytic actinobacteria from R. zoelleri which may be further assess for their novelty and bioprospecting.
EFFECT OF POVIDONE IODINE TREATMENT ON BACTERIAL COMMUNITY ASSOCIATED WITH WHITE SHRIMP (LITOPENAEUS VANNAMEI) LARVAE Lestari, Yulin; Suwanto, Antonius; Pangastuti, Artini; Suhartono, Maggy T.
Marine Research in Indonesia Vol 34, No 2 (2009)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/mri.v34i2.471

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The efficacy of povidone iodine as a surface disinfectant was assessed in white shrimp (Litopenaeus vannamei) eggs and larvae. Eggs and nauplii were exposed to povidone iodine 20 ppm for 20 seconds. Culture-based method and Terminal Restriction Fragment Length Polymorphism (T-RFLP) were used to monitor the total number of bacteria and diversity of the bacterial community associated with shrimp eggs and larva at each developmental stage. Povidone iodine reduced the total culturable bacteria, especially Vibrio, on eggs and nauplii, as well as the total bacteria in the whole community as estimated by T-RFLP results. Povidone iodine also reduced the diversity of bacterial community and altered the evenness of phylotypes distribution suggesting that the use of povidone iodine as a surface disinfectant in shrimp aquaculture should be reconsidered.
Characterization of Ethanolic Extract of Streptomyces sp. as a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12 Fitri, Lenni; Meryandini, Anja; Iswantini, Dyah; Lestari, Yulin
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8907

Abstract

Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of Streptomyces sp. AEBg12 endophytes. Supernatant of Streptomyces sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp Artemia salina. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic Streptomyces sp. AEBg12 contained flavonoids. IC50 value of ethanol extract was 180.83 g/ml. The result of TLC showed that ethanolic extract of Streptomyces AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of Streptomyces sp. AEBg12 was higher than wild bangle and bangle tissue culture. The information from this study can be be used as a basic data for further characterization of the active compound, which might be developed as an antiobesity agent through its pancreatic lipase inhibitory activity.
Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent Fitriandini, Risa; Budiarti, Sri; Lestari, Yulin
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10323

Abstract

Rhododendron has long been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria has not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from Rododendron spp. and assesed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from Rhododendron spp. using HV medium, and purified in ISP2 medium. The antibacterial activity was assayed against Enteropathogenic Escerichia coli (EPEC) K1.1 resistant to ampicillin and Bacillus pumilus. The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twenty three of endophytic actinobacteria were successfully isolated from 7 Rhododendron species. Two of them, i.e. RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against B. pumilus, respectively. The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 oC. At 250 g/mL to 1750 g/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against B. pumilus was at 1250 g/mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated B. pumilus showed elongated cells and viewer in cell number, compared with the untreated one. From this work, the existence of endophytic actinobacteria from Rhododendron spp. and their antibacterial activity contribute to the understanding of their diversity and potency as antibacterial agent.
Exploration of Potential Actinomycetes from CIFOR Forest Origin as Antimicrobial, Antifungus, and Producing Extracellular Xylanase Sipriyadi, Sipriyadi; Lestari, Yulin; Wahyudi, Aris Tri; Meryandini, Anja; Suhartono, Maggy Thenawidjaja
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5052

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This study aimed to isolate and explore the actinomycetes of CIFOR forest origin as an antimicrobial and antifungal agent, to produce an extracellular xylanase, and to identify isolates based on 16S rRNA gene sequences. Actinomycetes were isolated using Humic-acid Vitamin-B agar (HV) media. Actinomycetes colonies that grow on the medium HV was subsequently purified by growing them on yeast malt agar (YMA) media), then an antagonistic test of selected bacteria against Bacillus sp., Escherichia coli, Fusarium oxysporum, and Sclerotium sp was performed. Xylanase activity test was detected by observing a clear zone, followed by identification. Total of 35 isolates of actinomycetes isolated based on their colony morphology characteristics and diverse types of spore chains showed Streptomyces spp. of isolates CFR-06, CFR-15, CFR-17, CFR-18, and CFR-19 were able to inhibit the growth of Bacillus sp.. The highest inhibition zone has a diameter of 10.1 mm (isolate CFR-17). Isolates CFR-01 and CFR-15 were able to inhibit the growth of E. coli with the highest inhibition zone diameter of 5.1 mm (isolate CFR-15). Isolates CFR-29 and CFR-12 were able to inhibit the growth of F. oxysporum while isolate CFR-35 were able to inhibit the growth of Sclerotium sp.. Xylanase activity test showed that isolates CFR-12, CFR-20, CFR-22, CFR-24, CFR-25, CFR-30, CFR-33, CFR-34 have an ability to produce extracellular xylanase enzyme. Actinomycetes isolate (Xyl_22) as a potential xylanase enzyme producer was closely related with Streptomyces drozdowicii by the maximum similarity of 99%.How to CiteSipriyadi, S., Lestari, Y., Wahyudi, A., Meryandini, A., & Suhartono, M. T. (2016). Exploration Potential CIFOR Forest actinomycetes origin as Antimicrobial, Anti Fungus and Producing Enzymes Extracellular Xylanase. Biosaintifika: Journal of Biology & Biology Education, 8(1), 94-102.
Diversty of Actinomycetes from Soil Samples Collected from Lombok Island, Indonesia Lisdiyanti, Puspita; Tamura, Tomohiko; Ratnakomala, Shanti; Ridwan, Roni; Kartina, Gina; Lestari, Yulin; Katsuhiko, Ando; Widyastuti, Yantyati
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.783 KB) | DOI: 10.1234/52

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A total of 137 strains of Actinomycetes were isolated from 14 soil samples collected in 3 part of Lombok Island, Indonesia  using  SDS-YE (SY) and Rehydration and Centrifugation  (RC)  isolation methods  and Humic Acic Vitamin (HV) agar as isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. On the basis of their morphology and 16S rRNA gene sequence, 67% of isolates were belonged  to the Streptomyces Group and 33% of isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Nine families and 15 genera were found from the samples. It is indicated the richness of actinomycetes in these area. The genus  Streptomyces  is the most abundant in all soil samples, occupying 67% of all isolates by using SY method; while the genusActinoplanes  is mainly found by using RC as isolation method. Two strains belong to the genus Virgisporangium and Catenulispora that showed taxonomic interest need further study for describing as ne species.   Keywords: Actinomycetes, Streptomyces Group, Non-Streptomyces Group, Diversity
Antioxidant and Antiglycation Activity of Rhizosphere and Endophytic Actinobacteria of Xylocarpus granatum Ariansyah, Alifian; Batubara, Irmanida; Lestari, Yulin; Egra, Saat
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.478 KB) | DOI: 10.15294/biosaintifika.v11i2.20018

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Utilization of endophytic bacteria such as actinobacteria is one of the alternatives to obtain the bioactive compounds similar to the host plant. This study aimed to identify compounds produced by rhizosphere and endophytic actinobacteria isolated from Xylocarpus granatum for their antioxidant and antiglycation activity. Actinobacterial culture supernatant was extracted using ethyl acetate with a ratio of 1:1 (v/v). Antioxidant activity was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Whole TLC-bioautography and phytochemical tests were used to predict the group of active compounds. Antiglycation activity was identified based on the ability to inhibit the formation of advanced glycation end products (AGEs). Fifteen actinobacterial isolates had been isolated from rhizosphere (ten isolates), fruit (two isolates), and leaf (two isolates) of X. granatum. All isolates had been tested for their antioxidant and antiglycation activity. Isolate XR2 had the highest antioxidant activity with IC50 value of 1719.26 mg/L. Meanwhile, the highest antiglycation activity was obtained from isolate XR8 with IC50 value of 327.62 mg/L. This study informs that actinobacteria also live in the fruit, leaves and rhizosphere of X. granatum. The existence of rhizosphere and endophytic actinobacteria from X. granatum and their antioxidant and antiglycation activities contributes to the understanding of their diversity and potency as an antioxidant and antiglycation agent.
Co-Authors . Mahyarudin Abdjad Asih Nawangsih Amor Tresna Karyawati, Amor Tresna Anas, Iswandi Ando Katsuhiko ANDO KATSUHIKO Anja Meryandini Anja Meryandini Anja Meryandini Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R ARI SUSILOWATI Ariansyah, Alifian Ariansyah, Alifian Aris Tri Wahyudi ARTINI PANGASTUTI Arum Asiyanti Suhastyo, Arum Asiyanti Arum Asriyanti Suhastyo BAMBANG MARWOTO Batara, Lily Noviani Budi Wirawan Deden Saprudin DWI ANDREAS SANTOSA Dyah Iswantini DYAH NOOR HIDAYATI Egra, Saat Endang Sukara EVI TRIANA Evi Triana Ferry Mutia Fitriandini, Risa Fitriandini, Risa Gina Kartina GINA KARTINA GINA KARTINA Hamim Hamim HIDEKI YAMAMURA Iman Rusmana Indah Puspita SARI Irmanida Batubara Iswandi Anas Iswandi Anas KATSUHIKO ANDO Katsuhiko Ando Laksmita Prima SANTI Lenni Fitri Lia Aseptin Murdini Lilis Nuraida Maggy T. Suhartono Maggy Thenawidjaja Suhartono Marina Ratte Mazidah Noer Inayah Min Rahminiwati MISA OTOGURO Misa Otoguro MONA PRIMANITA Niken Financia Gusmawati NITA ROSALINDA PRAYITNO Nita Rosalinda Prayitno Nunuk Widhyastuti NUNUK WIDHYASTUTI PUSPITA LISDIYANTI Puspita Lisdiyanti PUSPITA LISDIYANTI PUSPITA LISDIYANTI Puspita Lisdiyanti RASTI SARASWATI RASTI SARASWATI RATIH DEWI HASTUTI RATIH DEWI HASTUTI Ratih Dwi Hastuti ROHMATUSSOLIHAT ROHMATUSSOLIHAT Roni Ridwan RONI RIDWAN RONI RIDWAN SASMIATI FARACH DITA Shanti Ratnakomala SHANTI RATNAKOMALA Shanti Ratnakomala SHANTI RATNAKOMALA SHINJI MIYADOH Sipriyadi Sri Budiarti Poerwanto Suryo Wiyono SUSILOWATI1 SUSILOWATI1 TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Titi Candra Sunarti dan Michael (E-Jurnal Agro-Industri Indonesia) TOMOHIKO TAMURA Tomohiko Tamura TOMOHIKO TAMURA TRI RATNA SULISTIYANI TRIO HENDARWIN Umi Fatmawati Winanda, Witri YANTYATI WIDYASTUTI Yantyati Widyastuti Yantyati Widyastuti Yantyati Widyastuti