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. PRIYONO
Indonesian Research Institute for Biotechnology and Bioindustry

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Propagasi in vitro tanaman kurma (Phoenix dactylifera L.) pada bioreaktor dengan perendaman sesaat Rizka Tamania SAPTARI; Masna Maya SINTA; Imron RIYADI; . PRIYONO; . SUMARYONO
E-Journal Menara Perkebunan Vol 88, No 2 (2020): Oktober,2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i2.394

Abstract

The cultivation of date palm in Indonesia has increased since the last decade. However, the superior date palm seedlings are still limited and most of them are imported from other countries. The mass supply of superior date palm seedlings can be provided by in vitro propagation in the bioreactor. Therefore, the research was conducted to develop a protocol of date palm in vitro propagation by using Temporary Immersion Bioreactor (TIB). The in vitro propagation was carried out through somatic embryogenesis technique using meristematic tissues isolated from offshoots of date palm female clone cv. Zambli as explants. The explants were sterilized and then cultured to produce embryogenic calli and somatic embryos. Afterwards, somatic embryos germination and plantlets formation were conducted in TIB with treatments of immersion period: 3, 10, and 30 minutes every 6 hours, with 8 replications, The results showed that the optimal somatic embryo germination in TIB was with the immersion period of 30 min every 6 h, resulting in the most formation of shoots and fresh biomass weight increment up to nearly threefold in 6 weeks. Thereafter, plantlets formation in TIB with immersion period of 10 min and 30 min every 6 h exhibited similar performances in producing more plantlets with higher total fresh weight and better vigor than those of 3 min every 6 h. However, there were more rooted plantlets in the TIB with immersion period of 10 min every 6 h. Based on the results, an in vitro propagation protocol via somatic embryogenesis in TIB has been successfully developed for mass propagation of date palm cv. Zambli, which produced plantlets with good vigor and rooting.
Respons tanaman tebu (Saccharum officinarum L.) terhadap aplikasi konsorsium biostimulan di tiga tipologi lahan Ciptadi Achmad YUSUP; Deddy PURWANTORO; Happy WIDIASTUTI; . SISWANTO; Djoko SANTOSO; . PRIYONO
E-Journal Menara Perkebunan Vol 89, No 2 (2021): Oktober, 2021
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i2.457

Abstract

The consortium biostimulant combines several types of biostimulant applied holistically, such as phytohormones to induce physiological processes, humic acid to improve nutrition intake and land fertility, and biofertilizer arbuscular mycorrhizal fungi to improve abiotic stress tolerance. The objectives of this research were to analyze the effect of application consortium biostimulant on the growth and productivity of Bululawang sugarcane variety planted in three land typologies, i.e. irrigated heavy soil with good drainage (BPL), irrigated heavy soil with poor drainage (BPJ), and rainfed light soil with good drainage (RHL). The research was conducted on plant cane (PC) sugarcane areal in Lumajang Regency, East Java, from July 2019 to September 2020. The treatment plot area was 1 ha for each land typologies, and the observation were conducted on 10 m plant row with ten times replications. Each treatment was replicated ten times. The results showed that the application of consortium biostimulant could induce faster growth of sugarcane shoots and better roots at one month after planting (MAP). Stalk height and diameter showed significantly different values between treatment and control at the plant age 6 to 12 MAP. In addition, the sugarcane stalk weight per meter row also increases by 13.72 – 28.57%. The growth performance of sugarcane on a commercial scale increased, also sugarcane productivity increased by 11.08 – 20.36%. The potential sugar yield increased by 15.05% in BPL land typology, 4.9% in BPJ land typology, and 9.7% in RHL land typology. The difference in land typologies affected the effectiveness of the consortium biostimulant application in increasing sugarcane productivity.
Peningkatan hasil panen kedelai (Glycine max L.) varietas Wilis dengan aplikasi biostimulan tanaman (Yield improvement of soybean (Glycine max L.) var. Wilis by the application of organic plant biostimulant) Dini Astika SARI; Irma KRESNAWATY; . PRIYONO; Asmini BUDIANI; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 87, No 1 (2019): April, 2019
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (817.538 KB) | DOI: 10.22302/iribb.jur.mp.v87i1.295

Abstract

The Indonesian government program of achieving self-sufficiency of soybean by 2020 requires technological innovations for the farmers. The use of plant biostimulant is an innovative strategy and proven previouslyto increase the productivity of several otherfood crops. The aim of this study was to analyze the effect of PPBBI biostimulant on the growth, productivity and quality of a Wilis variety of soybean under greenhouse conditions. PPBBI biostimulant at10 ppm and 20 ppm, was applied using foliar spray method with 20 mL volume to each plant. The applications were carried out 1 time, 2 times and 3 times. Six biostimulant treatments showed significant positive effects on the vegetative growth rate, generative organ development, and yield. The biostimulant accelerated vegetative growth to enter the generative phase earlier than that of in the control plants. The period of generative organs maturity required for treated plants was 7-14 d shorter than that of in the control plants so that the harvest period was 21 d shorter. Weight per 100 seeds of the P2-3 (application 3 times at 20 ppm); P2-2 (application 2 times at 20 ppm); P2-1 (application 1 time at 20 ppm) and P1-3 (application 3 times at 10 ppm) treatments were 20.16 g; 17.65 g; 18.89 g and 16.89 g respectively with no significant difference, while the control plants was only 11.60 g. Based on the results of all parameters e.g. average number of seeds, average weight per seed, and potential for yield improvement, the treatment of P1-3 (application 3 times at 10 ppm) was the best treatment with potential yield increase by 59.06% and oil content by 11.37%.[Key words: generative, organic biostimulant, productivity, vegetative]  AbstrakProgram pemerintah Indonesia dalam pencapaian swasembada kedelai pada tahun 2020 membutuhkan dukungan inovasi teknologi yang aplikatif untuk para petani. Biostimulan tanaman merupakan salah satu teknologi yang strategis dan terbukti dapat meningkatkan produktivitas beberapa tanaman pangan. Tujuan penelitian ini adalah untuk melakukan analisis pengaruh aplikasi biostimulan PPBBI terhadap partum-buhan, produktivitas dan kualitas hasil panen kedelai varietas Wilis pada kondisi rumah kaca. Biostimulan PPBBI dengan variasi konsentrasi   10 ppm dan 20 ppm, diaplikasikan pada tanaman kedelai dengan metode penyemprotan lewat daundengan volume 20 mL per tanaman.Penyemprotan dilakukan sebanyak 1 kali; 2 kali dan 3 kali aplikasi. Enam perlakuan biostimulanyang diujikan menunjukkan pengaruh positif yang signifikan pada kecepatan pertumbuhan vegetatif, stimulasi perkembangan organ generatif dan peningkatan hasil panen. Biostimulan PPBBI mempercepat laju pertumbuhan vegetatif untuk memasuki fase generatif lebih awal dibandingkan tanaman kontrol. Masa perkembangan dan pemasakan organ generatif polong pada tanaman perlakuan menjadi lebih pendek 7-14 hari dibandingkan tanaman kontrol sehingga secara keseluruhan masa panen tanaman perlakuan lebih singkat 21 hari. Bobot per 100 biji tanaman kedelai perlakuan P2-3; P2-2; P2-1 dan P1-3 berturut-turut mencapai 20,16 g; 17,65 g; 18,89 g; dan 16,89 g dengan tidak adanya perbedaan signifikan, sedangkan tanaman kontrol hanya 11,60 g. Berdasarkan hasil analisis seluruh peubah yaitu rerata jumlah biji, rerata bobot per biji, dan potensi produksi, maka perlakuan P1-3 (aplikasi tiga kali dengan dosis 10 ppm) merupakan perlakuan terbaik dengan potensi kenaikan produksi mencapai 59,06% dan kadar lemak 13,7%.  [Kata kunci: biostimulan organik, generatif, produktivitas, vegetatif]