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Yuke Rizky Amelia
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Veterinary

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Tingkat Maturasi in vitro Oosit Kambing dalam Medium dengan Suplementasi Serum dan Albumin Sri Gustari; Ni Wayan Kurniani Karja; Yuke Rizky Amelia; Ian Kurniawan; Bayu Sulistyo
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The present study aimed to study the effect of ?maturation media? on maturation rate of goat oocytesafter in vitro maturation. Goat ovaries were collected from a slaughter house in Godean, Sleman. Immediatelyafter slaughter the ovaries were collected, rinsed with physiological NaCl three times then placed in aflask containing the NaCl solutions and hept at 36-370C before transportaion to the laboratory. Oocyteswere observed under stereo microscope and its quality was classified into A, B, and C. Oocytes in vitromaturaion (IVM) was performed in TCM-199 media suplemented with : a) 0.4 mg/ml bovine serum albumin(BSA); and b) 10% newborn calf serum (NCS) then incubated at 38.50C with 5% CO2 for 24-27 h. Followingthis, oocytes were observed under inverted microscope for first polar body extrusion, then stained withaceto-orcein in order to evaluate nuck or maturation. The nuclear maturation stages including : germinalvesicles (GV), germinal vesicles break down (GVBD), metaphase I, apaphase I, telophase I and metaphaseII, respectivelly. The overall results showed that 74-74%, 52-66.6% and 21.5-23.8% of oocytes quality A, B,and C reached maturation at metaphase II, respectivelly. There were no significant differences in oocytesmaturation using media supplemented with either BSA or NCS.
Co-Authors Bayu Sulistyo Ian Kurniawan Ni Wayan Kurniani Karja Sri Gustari