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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner Ovozoa: Journal of Animal Reproduction Jurnal Kedokteran Hewan

Sri Gustari

Departemen Reproduksi Dan Kebidanan Fakultas Kedokteran Hewan Universitas Gadjah Mada

Author-ID : 385557

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Kualitas Morfologi Oosit Sapi Peranakan Ongole yang Dikoleksi secara In Vitro Menggunakan Variasi Waktu Transportasi Agung Budiyanto; Sri Gustari; Dito Anggoro; Dwi Jatmoko; Silvana Nugraheni; Eka Wahyu Nugraha; Donata Asta
Acta VETERINARIA Indonesiana Vol. 1 No. 1 (2013): Januari 2013
Publisher : IPB University

Salah satu alternatif usaha peningkatan populasi sapi di Indonesia adalah dengan transfer embrio. Kualitas oosit yang baik akan menghasilkan tingkat pembelahan dan blastosis yang baik. Lama waktu transportasi dari rumah potong hewan (RPH) ke laboratorium merupakan salah satu faktor yang dilaporkan berpengaruh terhadap kualitas oosit. Waktu transportasi yang tepat untuk menghasilkan oosit dengan kualitas morfologi terbaik belum pernah dilakukan untuk ovarium sapi di Indonesia. Penelitian ini dilakukan untuk mengetahui pengaruh lama waktu transportasi ovarium terhadap kualitas morfologi oosit sapi yang dikoleksi secara in vitro. Koleksi ovarium dilakukan di RPH, selanjutnya dibawa ke laboratorium untuk dilakukan proses koleksi oosit dengan metode aspirasi. Ovarium dikelompokkan berdasarkan waktu transportasinya, yaitu 2, 3, 4, dan 5 jam. Oosit yang diperoleh kemudian dikelompokkan berdasarkan kualitasnya dengan mengklasifikasikan menjadi kualitas A, B, C, dan D. Pada penelitian ini ditemukan adanya perbedaan yang signifikan antara lama waktu transportasi ovarium sapi terhadap kualitas morfologi oosit (P< 0,05). Dari penelitian ini dapat disimpulkan bahwa ovarium yang mengalami perlakuan transportasi selama 2 jam menghasilkan persentase jumlah oosit dengan kualitas morfologi A dan B yang lebih baik jika dibandingkan dengan ovarium yang mengalami perlakuan transportasi selama lebih dari 2 jam.

PENGARUH PEMBERIAN PROSTAGLANDIN F-2a DAN GONADOTROPHIN RELEASING HORMON TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH YANG MENGALAMI KASUS KAWIN BERULANG Surya Agus Prihatno; Sri Gustari
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Pengujian Daya Tahan Spermatozoa Semen Beku Kambing dan Domba setelah Thawing Sri Gustari
Jurnal Sain Veteriner Vol 21, No 1 (2003): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

INTRA-UTERIN ADMINISTRATION OF PROSTAGLANDIN FOR INDUCTION IN CROSS BREEDING GOATS Sri Gustari; Asmarani Kusumawati; Slamet Subagyo; Prabowo P Putro
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8622

Productivity and reproductivity of cross breeding goats is low. It is mainly caused by hormone deficiency and difficulties in estrus detection. The research studied the effect of intrauterine administration of prostagiandin on estrus induction and on number of pregnancies.Sixteen does were divided randomly into 2 groups. Group i were injected with prostagiandin intramuscularly. Group li were given prostagiandin by intrauterine administration. In both groups, prostagiandin were given twice with interval 11 days apart. The does which coming into estrus were mated naturally.There was a significantly different in the time of estrus between group I and group II (40.25 ± 4.94 and 80.8 ± 18.35 hours). On the other hand, there was no difference in estrus induction between those two groups (Group I : 87.5% and Group II : 62.5 %). All the does which were mated naturally were pregnant (100%). Although time to estrus after intrauterine administration of prostagfandin was longer than that of intramuscular ones, but it was a cheaper method.

THE USE OF DIFFERENT STAINING METHODS TO EVALUATE THE DEVELOPMENT AND VIABILITY OF BOVINE EMBRYOS OBTAINED IN VIVO AND IN VITRO Sri Gustari; Julian A.Bartolome Julian A.Bartolome Julian A.Bartolome
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

In an attempt to find a good method to evaluate embryos we classified the in vitro embryos into freezable and non-freezable by subjective method. These embryos were compared with in vivo freezable embryos using Giemsa staining. The embryo development and viability was evaluated by fluorescent stain (PDA and Hoechst 33342) and compared with the result after Giemsa stain.That number of cells in freezable in vitro and in vivo embryos were not different (P > 0.1), while in vitro fresszable and non-freezable embryos showed significantly differences (P < 0,001) in number of cells.The agreement between Hoechst 33342 and Giemsa stain was quite high (63%) in embryos with number of cells less than 20, and low agreement (25 - 34 %) in embryios with number of cells more than 20. There was a significantly correlation (0.82) betwen the degree of fluorescent of FDA stain and the number of cells at Giemsa stain.The subjective classification of in vitro embryos can be used in the practical situation. PDA staining is a good tool to check this classification as well as to evaluate the development and viability of embroys in different step of experimental trials. Hoechst can be a valuable

Pengaruh Pemberian GnRH pada Sapi Potong yang Mengalami Kawin Berulang (Repeat Breeding) Surya Agus Prihatno; Sri Gustari; Asmarani Kusumawati; Agung Budiyanto; Erif Maha Nugraha Setyawan; Yosua Kristian Adi
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.62761

Repeat breeding is a condition in which cows have normal estrous cycles and have been mated 3 times or more but are not pregnant. The incidence of repeat breeding in beef cattle can be detrimental to breeders in terms of time, that the calving period becomes longer so that the cows cannot give birth to calves once a year. Various ways to deal with repeat breeding have been done, but the results are not optimal. This study was conducted to determine the effect of Gonadotrophin Releasing Hormone (GnRH) in cows with repeat breeding. A total of fifteen beef cattle with repeat breeding were used in this study. The cows were divided into 3 groups of 5 each. Cows that were estrus in the first group (as a control group) were only performed artificial insemination (AI) without GnRH injection. In the second and third groups, after AI, they were injected with GnRH at AI time and 3-4 days after AI with a dose of 5 ml (Fertagyl®, 500 µg gonadorelin) intramuscularly. A pregnancy examination was carried out three months later and the results were recorded. The data obtained were analyzed descriptively and quantitatively using Chi-Square statistical analysis. The results of the analysis showed that there were differences in the pregnancy rate in the first group (control) with the second cow group and the third cow group, which were 20%, 80%, and 60% respectively. Based on statistical analysis, there was a significant difference in pregnancy rate (P <0.05) between the three groups. It was concluded that GnRH administration could increase the pregnancy rate in beef cattle that had repeat breeding.

Fetal Bovine Serum Meningkatkan Maturasi Inti Oosit Kelinci Setelah Dimaturasi Secara In Vitro Ni Wayan Kurniani Karja; Winny Plumeria Aqshani; Yesi Pratiwi Kusumawati; Veronika Gilang Pravitasari; Sri Gustari
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.921 KB)

This study was conducted to examine the meiotic competence or nuclear maturation of rabbit oocytesmatured in vitro. Oocytes were recovered by mincing the ovaries in modified phosphate buffer saline (m-PBS). Selected cumulus-oocyte complexes (COCs) with compact cumulus cell mass and a dark, homogenousooplasm were cultured in maturation medium at 38°C in a humidified atmosphere of 5% CO2 in air, andthen stained for nuclear maturation. Three experiments were carried out. In Experiment 1, COCs werecultured in maturation medium for 18-20, 22-24, and 28-30 h. The proportion of oocytes at metaphase II(MII) was similar (P>0.05) at 18-20 (69.2%), 22-24 (66.4%), and 28-30 (71.1) h of culture. In Experiment 2,COCs were cultured in either maturation medium containing 0.04% bovine serum albumine (BSA) or 5%fetal bovine serum (FBS) for 24 h. The proprotion of oocytes that reached MII were higher (P<0.05) in FBSgroup (79.2%) than those of oocytes in BSA group (64%). In Experiment 3, based on the presence or absenceof follicles and corpora lutea, the ovarian pairs were classified into follicular or luteal stages. There was nodifference (P>0.05) among oocytes collected from ovaries in follicular (79.7%) and luteal stages (78.7%) inthe ability to achieve nuclear maturation. These results indicated that nuclear maturation of rabbitoocytes in vitro was completed at 18-20 h of maturation culture and was not affected by ovary’s reproductivestage. The presence of FBS in the maturation medium enhanced the ability of rabbit oocytes to achievenuclear maturation.

Tingkat Maturasi in vitro Oosit Kambing dalam Medium dengan Suplementasi Serum dan Albumin Sri Gustari; Ni Wayan Kurniani Karja; Yuke Rizky Amelia; Ian Kurniawan; Bayu Sulistyo
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.622 KB)

The present study aimed to study the effect of ?maturation media? on maturation rate of goat oocytesafter in vitro maturation. Goat ovaries were collected from a slaughter house in Godean, Sleman. Immediatelyafter slaughter the ovaries were collected, rinsed with physiological NaCl three times then placed in aflask containing the NaCl solutions and hept at 36-370C before transportaion to the laboratory. Oocyteswere observed under stereo microscope and its quality was classified into A, B, and C. Oocytes in vitromaturaion (IVM) was performed in TCM-199 media suplemented with : a) 0.4 mg/ml bovine serum albumin(BSA); and b) 10% newborn calf serum (NCS) then incubated at 38.50C with 5% CO2 for 24-27 h. Followingthis, oocytes were observed under inverted microscope for first polar body extrusion, then stained withaceto-orcein in order to evaluate nuck or maturation. The nuclear maturation stages including : germinalvesicles (GV), germinal vesicles break down (GVBD), metaphase I, apaphase I, telophase I and metaphaseII, respectivelly. The overall results showed that 74-74%, 52-66.6% and 21.5-23.8% of oocytes quality A, B,and C reached maturation at metaphase II, respectivelly. There were no significant differences in oocytesmaturation using media supplemented with either BSA or NCS.

Pengaruh penggunaan madu sumbawa dan madu olahan berbagai konsentrasi terhadap kualitas spermatozoa epididimis domba setelah penyimpanan dalam refrigerator Siti Diyani Ludfiyaningrum; Sri Gustari
Ovozoa : Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.1-6

Artificial Insemination is used to conserve genetic resources. It is necessary to preserve spermatozoa in a certain diluent at certain temperature before artificial insemination. Information regarding the use of honey in the spermatozoa diluent is still less in number. This research aims to determine the effect of Sumbawa honey and processed honey in various concentrations on the quality of spermatozoa after storage in refrigerator. The main materials used were five ram testicles. Spermatozoa was collected from cauda epididymis by mincing and adding 5 ml PBS. The sample were divided into 7 groups of treatment: 1% Sumbawa honey, 2% Sumbawa honey, 3% Sumbawa honey, 1% processed honey, 2% processed honey, 3% processed honey, and control using 0.5 gram glucose in 100 ml Tris diluent. The assessment was carried out microscopically including motility and viability. Data were analyzed statistically using one-way analysis of variance (Anova) on the SPSS statistics 16.0 program. The results showed no significant difference (p > 0.05) in the motility and viability of spermatozoa in all treatment of Sumbawa honey (1%, 2%, 3%), processed honey (1%, 2%, 3%), and control. The conclusion of this study is that honey in spermatozoa diluent has no effect on the quality of spermatozoa.

TEPUNG BEKICOT (Achatina fulica) SEBAGAI PAKAN TAMBAHAN UNTUK MENINGKATKAN KUALITAS SEMEN DOMBA (Ovis aries) Jayanti Berliana Dewi; Delvi Ramadayani; Marchellia Dhiafifah Wahyunandha; Melati Kusuma Bunda Pertiwi; Sri Gustari; Topas Wicaksono Priyo Jr
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69114

One of the effort to provide superior rams is to pay attention to the provision of feed that has good substances and is able to improve the quality of sperm. Based on studies, snail flour has a fairly high protein content and snails can be an alternative source of protein in additional feed. This study aims to improve the quality of sheep sperm using an additional feed of snail flour (Achatina fulica) which can increase the use value and economic value. Eight sheep used in this research were divided into one group as the control and two groups as the treatment. The control group was given standard sheep forage, while the treatment groups were given standard sheep forage added with flour snail of 5% and 10%. Sperm collection using castration method. Parameters observed were motility, viability, and concentration of sperms. The results of the study showed that the addition of snail flour as additional feed for sheep affected sperm quality with a significant increase in concentration parameters, while motility and viability did not show a significant increase

Co-Authors Agung Budiyanto Agung Budiyanto Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bayu Sulistyo Delvi Ramadayani Dito Anggoro Donata Asta Dwi Jatmoko Eka Wahyu Nugraha Erif Maha Nugraha Setyawan Erif Maha Nugraha Setyawan Ian Kurniawan Jayanti Berliana Dewi Julian A.Bartolome Julian A.Bartolome Julian A.Bartolome Lalu Unsunnidhal Marchellia Dhiafifah Wahyunandha Melati Kusuma Bunda Pertiwi Mujahidatul Musfiroh Ni Wayan Kurniani Karja Prabowo P Putro Silvana Nugraheni Siti Diyani Ludfiyaningrum Slamet Subagyo Surya Agus Prihatno Topas Wicaksono Priyo Jr Veronika Gilang Pravitasari Winny Plumeria Aqshani Yesi Pratiwi Kusumawati Yosua Kristian Adi Yuke Rizky Amelia

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