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Aspek-aspek Reproduksi Cacing Diopatra neapolitana Di Cilacap Ita Purwati; Farida Nur Rachmawati; Eko Setio Wibowo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3494

Diopatra neapolitana merupakan polychaeta yang memiliki tubuh dengan struktur tabung besar. D. neapolitanan dapat dimanfaatkan sebagai pakan udang karena memiliki kandungan nutrisi yang cukup tinggi. Penggunaan D. neapolitana sebagai pakan dilakukan dengan mengambil cacing langsung dari alam sehingga dapat mengganggu keberlangsungan populasi cacing D. neapolitana di alam. Kondisi ini memerlukan adanya usaha domestifikasi dan budidaya D. neapolitana, namun informasi tentang kondisi biologis dan reproduksi yang ada masih terbatas, terutama spesies yang ditemukan di Cilacap. Penelitian ini bertujuan untuk mengetahui aspek-aspek reproduksi D. neapolitana yang dapat mendukung domestifikasi dan budidaya cacing D. neapolitana. Hasil penelitian menunjukkan nilai rasio jenis kelamin yang seimbang yaitu 1:1. Ukuran tubuh cacing betina dan jantan tidak berbeda nyata. Hasil pengamatan pada cacing betina menunjukkan rata-rata jumlah segmen 131,96 ± 28,19 buah; rata-rata berat tubuh 2.65 ± 1,15 g dan cacing berada dalam fase mature dan submature dengan diameter telur antara 199-240 µm. Cacing jantan memiliki rata-rata jumlah segmen 136,97 ± 24,51 buah; rata-rata berat tubuh 2.18 ± 1,11 g dan bentuk sperma berupa tetrad spermatid. Hasil analisis hubungan berat tubuh dan tingkat maturasi D. neapolitana menunjukkan hasil korelasi negatif dengan r= 0,009, persamaan regresi linier y= 219.868 – 0,080x; dan nilai t hitung (-0,043) < t tabel (2,07961), dan asil analisis hubungan jumlah segmen dan tingkat maturasi D. neapolitana didapat nilai r = 0,101; r2 = 1%; persamaan regresi linier y = 224.247– 0,035x; dan t hitung (-0,463) < t tabel (2,07961). Hasil analisis regresi linier menunjukkan bahwa %. Berat tubuh tidak berkorelasi positif dengan tingkat maturasi cacing D. neapolitana. Peningkatan berat tubuh tidak selalu dikuti dengan peningkatan tingakat maturasi, dan peningkatan jumlah segmen tidak selalu diikuti dengan peningkatan tingkat maturasi cacing D. neapolitana.

PENGARUH SUPLEMENTASI Chlorella vulgaris PADA PAKAN UNTUK MENINGKATKAN ALBUMIN DALAM SERUM IKAN NILEM (Osteochilus vittatus) Kiki Siti Zakiah; Sorta Basar Ida Simanjuntak; Eko Setio Wibowo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2144

Nilem fish belongs to freshwater fish commodity that has been widely cultivation. Some factors influencing fish cultivation are good quality of feed, quantity, size, and shape. Quality feed works as a primary energy source as well as improves the digestive system of fish, with the expectation that the fish growth and health are optimal. Information concerning health status in cultivation Nilem has been used as a reference for improving cultivation methods and controlling disease attacks. The population and production of fish cultivation is experiencing serious problems due to infection from various types of pathogens. Therefore, a viable alternative is needed to prevent and overcome diseases by utilizing immunostimulant substances derived from synthetic substances that can increase the activity of non-specific immune defenses and stimulate antibiotic-forming organs in fish body. Immunostimulant is a substance that is capable of increasing the mechanism of non-specific immune response of fish one of them is by adding Chlorella vulgaris extract to the feed. This research objective is to determine the influence of Chlorella vulgaris supplementation on total blood albumin in nilem fish. This research was conducted by sampling method consisting of 5 treatments with further 4 replications. The treatments were feed supplementation of 0 g.kg-1, 2 g.kg-1, 3 g.kg-1, 4 g.kg-1, and 6 g.kg-1. Independent and dependent variables were used in this research; the independent variable was the dose of Chlorella vulgaris in the feed and the dependent variable was blood albumin concentration. The measuring of plasma albumin concentration was done using the Dyasis kit method with a spectrophotometer. The obtained data were then analyzed by ANOVA, followed by the Least Significant Difference (LSD) at the 95% confidence level if there is a real difference then followed by Tukey's test at the same confidence level. The results showed that the average value of serum albumin concentration of nilem fish from all treatments was between 4.2761 – 7.1826 g/dL. The normal albumin concentration was found in the treatment 4 g.kg-1 Chlorella vulgaris supplementation which was 4.77 g/dL. Meanwhile, the highest concentration was found from the supplementation of Chlorella vulgaris at 3 g.kg-1 feed which was 6.677 g/dL.

Effect of Batik Wastewater Resulted from Biosorption Process to Blood Glucose Level on Carp (Cyprinus carpio) Safira Dwi Oktaviani; Sri Lestari; Eko Setio Wibowo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Chromium from the process of coloring batik has high toxicity to the organism. The biosorption method is the heavy metal adsorption process using inactive organisms to eliminate or decrease chromium in batik wastewater. In a polluted environment, fish can be in a stressful condition that requires energy to adapt to environmental changes; high energy requirements will stimulate an increase in blood glucose levels. Chromium exposure can increase the blood glucose level on carp (Cyprinus carpio). This study aims to determine the effect of batik wastewater resulted from the biosorption process exposure to the blood glucose levels. This study used 4 concentrations with 6 replications. Carp is placed in a mixed water tank with batik waste resulting from biosorption. The treatment of batik wastewater produced from the biosorption process concentration are of 0%v.v-1; 39,657%v.v-1; 26,438%v.v-1; and 13,219%v.v-1. Research shows that chromium was decreasing the results obtained at K0 are 68 mg/dL, at K1 is 132 mg/dL, K2 is 127 mg/dL, and at K3 is 116 mg/dL Batik wastewater resulted from biosorption process increasing the blood glucose level in fish and accelerates the rate of expenditure on fish The optimaly concentration of batik wastewater resulted from biosorption process are tried can Keyword : Batik wastewater, Biosorption, Blood glucose level, Carp

Toksisitas Subletal Limbah Cair Batik Hasil Biosorpsi terhadap Hematokrit Ikan Mas (Cyprinus carpio) Nur Hidayati; Sri Lestari; Eko Setio Wibowo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Research on the hematocrit value of fish exposed to batik wastewater resulted from the biosorption process needed because the blood is one indicator of contaminants present that entering the fish body. This research aims to determine the effects of batik wastewater resulted from biosorption process exposure on Carp (Cyprinus carpio) hematocrit value. The treatments are batik wastewater resulted from biosorption process with different concentrations, i.e 0; 1,32; 2.64 and3, 96 % v.v-1. The carp maintained for 7 days and the blood was taken in the 8 days. Measured variables were hematocrit value. The result shows that the highest of the hematocrit in 0%v/v-1 concentration was 31.67±1.49 % while the lowest in 3,96 % v.v-1 concentration was 26.00±1.15 %. The conclusion, that batik wastewater resulted from the biosorption process induced a significant increase in the hematocrit value.

Potensi Reproduksi Ikan Air Tawar Sebagai Baby Fish Nuning Setyaningrum; Eko Setio Wibowo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.2.475

Informasi dasar mengenai potensi reproduksi ikan dapat diperoleh dari tinjauan fenomena perkembangan gonad, untuk memprediksi proses reproduksi sampai ikan memijah dan menghasilkan benih sebagai baby fish. Tujuan penelitian adalah mengetahui fekunditas, diameter telur, gonado somato index (GSI) dan kelangsungan hidup larva. Metode yang digunakan survey, pengambilan sampel puposive random. Materi penelitian adalah induk Cyprinus carpio, Barbonymus gonionatus, Osteochillus vittatus, Oreochromis niloticus dan Clarias gariepinus, sebanyak 3 pasang/species. Variabel adalah fekunditas, diameter telur, gonado somato index (GSI) dan kelangsungan hidup larva. Parameter yang diukur berat ikan, berat gonad, jumlah telur sebagian, ukuran telur dan jumlah larva hidup. Metode penelitian survey, pengambilan data adalah puposive random dan data dianalisis dengan Anova. Hasil penelitian diperoleh bahwa fekunditas dan GSI pada 5 species ikan menunjukkan perbedaan (P<0,05), B. gonionotus memiliki fekunditas tertinggi rata-rata 182.320 butir dan O. vittatus memiliki nilai GSI tertinggi 35,13%. Diameter telur berbeda-beda pada semua spesies, B. Gonionotus memiliki diameter terkecil yaitu kisaran 0,486 – 0,729 mm, sedangkan diameter terbesar pada ikan C. carpio rata-rata 1,170 mm. Larva O. Niloticus menghasilkan prosentase kelangsungan hidup terbaik sebesar 97%, O. vittatus 93%, C. carpio 77,2%, C. gariepinus 51,6%, B. gonionotus 31% dan uji Least Significant Difference larva O. niloticus dan O. vittatus memiliki kelangsungan hidup sama baik, berpotensi dikembangkan sebagai baby fish. O. vittatus memiliki peluang besar dikembangkan menjadi baby fish sampai ukuran 5 g/ekor dengan fekunditas rata-rata 16.250 butir.

Histopatologi Hepar Mencit (Mus musculus) Jantan yang Diberi Air Rebusan Plastik terhadap Antioksidan Alami Nuridha Audinia Safitri; Atang Atang; Eko Setio Wibowo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4606

Plastic in Indonesian daily life cannot be avoided anymore. Materials for the manufacture of plastic can cause free radicals to the body, thus affecting the immune system. These materials are including phthalates and bisphenol-A (BPA), which commonly called as plasticizers. Free radicals in the body can be inhibited with antioxidants. Antioxidants can be obtained from endogenous (inside the body) and exogenous (outside the body). The purpose of this study was to determine the damage level of liver histopathological structure as the result of plastic boiled water induction towards natural antioxidants feeding on male mice (Mus musculus). This study used an experimental method with a completely randomized design (CRD) consisting of six treatments and five replications for 21 days. The treatments included two control groups and four other groups treated with plastic boiled water and natural antioxidants. The independent variable is natural antioxidants and the dependent variable is the liver histopathology of male mice. The qualitative parameters observed were the liver histopathological structure of male mice is in the state of inflammatory cell infiltration, degeneration, and necrosis. The research data is an overview of the liver histopathological structure of male mice. The obtained data were analyzed using descriptive qualitative analysis method. Oral induction of boiled-plastic water can affect the changes of liver histological structure. Natural antioxidants given in male mice can maintain the tissue structure as the result of boiled-plastic water induction. The results showed the damage level of male mice liver histopathological structure as reversible degeneration.

Diferensial Leukosit Beberapa Spesies Ikan Tangkapan Dari Familia Cyprinidae Di Sungai Banjaran, Kabupaten Banyumas Nabilla Gina Irwanti; Priyo Susatyo; Eko Setio Wibowo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4703

Familia Cyprinidae dapat ditemukan di hampir seluruh sungai Indonesia termasuk Sungai Banjaran, Kabupaten Banyumas. Diferensial leukosit bertujuan mengetahui perbedaan persentase sel leukosit. Penelitian ini untuk mengetahui hubungan antara lokasi pengambilan sampel (hulu, tengah, dan hilir) dengan diferensial leukosit dan mengetahui gambaran apusan darah dari beberapa spesies ikan familia Cyprinidae. Pengambilan sampel menggunakan teknik purposive sampling pada tiga stasiun yang berbeda. Data yang diperoleh dianalisis dengan metode Analysis of Variance (ANOVA) dengan tingkat kesalahan 5% dan gambaran hasil pengamatan mikroskopis jenis-jenis leukosit. Hasil penelitian menunjukkan bahwa perbandingan persentase sel lekosit pada Ikan Nilem (Osteochilus vittatus), Ikan Brek (Barbonymus balleroides), dan Ikan Lunjar (Rasbora argyrotaenia) dengan lokasi Hulu, Tengah, Hilir Sungai Banjaran, Banyumas, berbeda secara signifikan (p<0,05). Persentase sel leukosit yang paling tinggi ada pada zona Hilir di Sungai Banjaran, Banyumas yaitu sel neutrofil dan sel monosit. Presentase sel neutrofil Ikan Nilem didapatkan sebesar 9,5%, Ikan Brek sebesar 8,5% dan Ikan Lunjar sebesar 9,4%. Presentase sel monosit pada Ikan Nilem sebesar 8,9%, Ikan Brek sebesar 8,7% dan Ikan Lunjar sebesar 8,5%. Sel neutrofil memiliki sitoplasma bergranula halus dan ditengahnya terdapat inti bersegmen. Sel eosinofil mempunyai sitoplasma berwarna lebih merah, inti yang terletak memanjang di tepi sel, memiliki granula besar. Sel limfosit memiliki sitoplasma berwarna biru gelap dan inti berbentuk bulat hingga oval. Sel monosit memiliki sitoplasma berwarna biru pucat dan bentuk inti bervariasi ada yang berbentuk seperti ginjal hingga yang berbentuk dua lobus besar.

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