Claim Missing Document
Check
Articles

Found 16 Documents
Search

TINJAUAN HISTOLOGIS PEMBULUH DARAH TIKUS PUTIH (Rattus norvegicus) DIABETES YANG DIBERI REBUSAN DAGING BUAH MAHKOTA DEWA (Phaleria macrocarpha (Scheff.) Boerl.) Sulistyoningrum, Evy; Kautsari, Septina; Susatyo, Priyo
MANDALA of Health Vol 4, No 2 (2010): Mandala Of Health
Publisher : Jurusan Kedokteran FK Unsoed

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Prevalence of Diabetes Mellitus among adult in Indonesia reached 4,1% in 1995 and projected to 6,5% in 2025. Complication of diabetes mellitus which come earlier and widely distributed yis atherosclerosis.Atherosklerosis can lead to other serious complications such as gangrene, stroke, cardiomiopathy, andcoronary artery diseases. This research was aimed to know the effect of Mahkota dewa (Phaleriamacrocarpha (Scheff.) Boerl.) boiled water to regeneration of aorta’s atherosclerosis post diabetic inductionwith alloxan. The research was conduct in four weeks to 25male albino rats (Rattus norvegicus) 3 monthaged, 120-180 grama weighed. The subjects was divided into five groups:positive control, negative control,A, B and C. A, B and C groups was treated Mahkota dewa boiled water with 4,5%, 9%, and 13,5%concentration respectively. The drug was administered orally. III, IV, and V groups are injected by alloxan toinduce diabetes. Atherosclerosis frequency was counted from histological section stained with Haematoxylineosin.Statistical analysis using Chi square analysis showed that Mahkota Dewa boiled water significantlyreduced atherosclerosis frequency in aorta. It can be concluded that Mahkota dewa (P.macrocarpha(Scheff.) Boerl.) regenerates atherosclerosis injury in diabetic blood vessel.
Effects of Some Feed Suplements Types to the Growth of Javaen Barb/Brek Fish (Puntius orphoides) Second Filial as Domestication Product Susatyo, Priyo; Sugiharto, Sugiharto; Hana, Hana; Chasanah, Titi
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5517

Abstract

Aquaculture can be improved by a diversified approach in the form of domestication research of a wild fish. The research has been done by cultivating Javaen Barb/Brek (Puntius orphoides) from Serayu river, Banyumas at a natural pond and laboratory scale. Brek fish is relative slow in growth and development of gonad. This research is aimed to determine: (1) the rate of general growth of Brek in the provision of some kinds of additional food; (2) Relative Growth Rate (RGR); (3) Specific Growth Rate (SGR) and Feed Conversion (FC). The research used experimental methods. The method was a completely randomized design (CRD) of 4 treatments and 3 replicationsof: A = 100% pellet; B = 70% soybean sprout and 30% pellet; C = 70% Ipomoea aquatica leaf and 30% pellet; D = 35% soybean sprouts, 35% I. aquatica leaf and 30% pellet. Quantitative data were analysed by analysis of variance (ANOVA) of the fishes growth.The results show influence of feed types to the Relative Growth Rate (RGR) of Javaean Barb. The best RGR with the value of 72.40 was given by feed types in the D combination. Meanwhile, Specific Growth Rate (SGR) and Feed Conversion (FC) were not affected by the feed types. The study were expected to provide suggestions for efficient feeding techniques in attempts at domestication of wild fish.How to CiteSusatyo, P., Sugiharto, S., Hana, H., & Chasanah, T. (2016). Effects of Some Feed Suplements Types to The Growth of Javaen Barb/Brek Fish (Puntius orphoides) Second Filial As Domestication Product. Biosaintifika: Journal of Biology & Biology Education, 8(3), 278-285.
Antioxidant Effect of Clorella vulgaris on Wistar Rat Kidney Induced by CCl4: A Histopathological Review Susatyo, Priyo; Rifanda, Achmad Akbar; Simanjuntak, Sorta Basar Ida; Chasanah, Titi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13398

Abstract

Kidney is very susceptible to damage by toxicological compounds such as carbon tetrachloride (CCl­4). CCl4 produce free radicals, which cause lipid peroxidation and kidney damage and free radical release, which can be prevented by the administration of exogenous antioxidants, such as Chlorella vulgaris.The aim of study was to determine an effect of antioxidant of C. vulgaris on the histopathological features of Wistar rat  kidney which is induced by CCl4. Experimental study with completely randomized design. The variable was histopathology features of the kidneys. The doses of C. vulgaris extract were 3 mg, 4 mg, and 5 mg per 100 grams of rat body weight (BW). The administration of C. vulgaris extract was performed within 30 days, while the CCl4 induction (0.25 ml / 100 g BW) was administered orally on the day 9, 12, 16, 19, 23, and 26. Parameters were histopathology features of renal damage, proportion of tubular cell damage, and Bowmans space diameter. The results showed the administration of C. vulgaris extract was able to reduce the impact of damage caused by CCl4 (p<0.05). This was supported by histologic observations, which was showing a decrease of picnotic and vacuolated cells, normal brush border, and decrease of Bowmans space. In conclusion, 5 mg / 100 g BW of C. vulgaris extract can effectively protect the kidney from damage        caused  by        CCl4. The results of this study strongly support further research on immunostimulant content test C. vulgaris and determine the efficient dose for representative animals mammals also in humans.
Reproduction Characteristics of Rice Field Eel (Monopterus albus Zuieuw) on Several Functionally Changed Lands in Banyumas Regency Susatyo, Priyo; Setyaningrum, Nuning; Winarni, Elly Tuti; Chasanah, Titi; Atang, Atang
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.976 KB) | DOI: 10.11594/jtls.08.02.12

Abstract

Functional change of agricultural lands into non-agricultural lands will obviously have an impact on the dimin-ished or loss of habitat of the rice field eels (Monopterus albus Zuieuw). The aim of this research was to un-derstand the difference between (1) reproduction parameters (fecundity, gonadal maturity level (GML), and gonadal maturity index (GMI); (2) histological features of female and male gonadal development (oogenesis and spermatogenesis); (3) range of body length in each sexual phase; and (4) range of body length in each GML stage of captured eel from 4 sub-districts of Banyumas regency, Central Java (Ajibarang, Sumpyuh, Banyumas, and Sumbang). The total number of captured subjects were 631 eels; which consisted of 155; 227; 157; and 92 eels from Ajibarang, Sumpyuh, Banyumas, and Sumbang, respectively. We observed oogenesis in the stages of GML-1 (chromatin nucleolar stages and perinucleolar stage); GML-II (cortical alveolar formation stage or globular yolk stage), GML-3 (late globular yolk stage), GML-IV (mature or ripe stage), and follicle atretic stage. Also, we observed male gonadogenesis and spermatogenesis in the intersex. The range of GMI of captured eels was between 0.023 and 0.686. Eel in GML-I, GML-2, GML-3, and GML-IV had GMI within the range of 0.023-0.096; 0.096-0.425; 0.427-0.686; and >0.686, respectively. Eels achieved stage of GML-IV in body length range of 22.6-34.5 cm in Sumpyuh, 22.6-38.5 cm in Ajibarang, and 26.6-34.5 cm in Banyumas. There was no eel with the status of GML-IV in Sumbang, which had the most residential or functionally changed lands in its area. Based on these findings, can be concluded that the condition of rice fields or habitat of eel in functionally changed lands may affect eel’s growth and gonad maturation.
AKTIVITAS ANTI MAKAN EKSTRAK DAUN SIRSAK (ANNONA MURICATA L.) DAN PENGARUHNYA TERHADAP INDEKS NUTRISI SERTA TERHADAP STRUKTUR MEMBRAN PERITROFIK LARVA INSTAR V SPODOPTERA LITURA F. Trisnowati B. Ambarningrum; Endang A. Setyowati; Priyo Susatyo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 2 (2012): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2019.948 KB) | DOI: 10.23960/j.hptt.212169-176

Abstract

Antifeeding activity of soursop leaf extract (Annona muricata L.) and its effect on nutrition indices and the microscopic structure of peritrophic membrane of the fifth-instar larvae of Spodoptera litura F. were studied. Antifeeding test was conducted by choice method. Discs of caisin leaf were dipped into the ether fraction of soursop leaf extract with concentrations of 0; 0.63; 2.50; and 10.00%. Nutrition indices and peritrophic membrane structure were observed by giving fifth-instar larvae one of the five diets, every group of diet was added to various concentrations of the ether fraction of soursop leaf extract, containing either 0; 0.63; 1.25; 2.50; and 5.00%. The result showed that soursop leaf extract had antifeeding activity at tested concentration 2.50%. Relative consumption rate (RCR), relative growth rate (RGR), and efficiency of conversion of ingested food (ECI) were significantly lower in the case of treated larvae than that of the controls. However the efficiency of conversion of digested food (ECD), approximate digestibility (AD), and peritrophic membrane structure treated with soursop leaf extract were not significantly affected as compared to those in controls.
Hubungan beberapa Aspek Biologi Reproduksi dengan Panjang Tubuh Ikan Palung (Hampala macrolepidota Kuhl & Van Hasselt 1823) di Waduk Panglima Besar Jenderal Soedirman Dhita Rasyida Soraya; Priyo Susatyo; Siti Rukayah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3481

Abstract

Penelitian ini bertujuan untuk mengetahui mengetahui rasio kelamin, TKG, IKG, fekunditas, ukuran pertama kali matang gonad ikan palung (H. macrolepidota) yang tertangkap di Waduk Panglima Besar Jenderal Soedirman dan hubungan antara beberapa aspek biologi ikan palung dengan panjang tubuh. Pengambilan sampel ikan dilakukan dua kali pada November dan Desember 2017. Hasil penelitian menujukkan bahwa ikan palung di Waduk Panglima Besar Jenderal Soedirman memiliki rasio kelamin 1 : 2,82. Tingkat Kamtangan Gonad (TKG) ikan palung yang ditangkap berada pada TKG I-IV. Rata-rata ukuran pertama kali matang gonad pada ikan palung betina adalah 447,6 mm dan ikan palung jantan 295,8 mm. Fekunditas ikan palung sebanyak 841-35.896 butir. Hubungan TKG dengan panjang total pada ikan betina berkorelasi positif (r = 0,827) dan jantan memiliki korelasi positif (r = 0,566). Hubungan IKG dengan panjang total ikan betina berkolerasi positif (r = 0,670). Hubungan IKG dengan panjang total ikan jantan berkolerasi positif (r = 0,392). Hubungan fekunditas dan panjang total memiliki korelasi positif (r = 0,978)
Status Reproduksi Belut Sawah (Monopterus albus) yang tertangkap di Desa Kalitinggar, Kabupaten Purbalingga Sa'adatul Amalia; Priyo Susatyo; Sugiharto Sugiharto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3393

Abstract

Ricefield eel is one type of fish whose habitat is in paddy fields and the eel's body does not have scales. Ricefield eel has a high protein, so it is widely consumed and sold in local and international markets. Eel reproduction is a protogynous hermaphrodite thathas a replacement cycle from female sex to the male sex. This research was conducted in the laboratory of the Faculty of Biology, Jenderal Soedirman University, by taking samples of Ricefield Eel in Kalitinggar Village, Padamara District, Purbalingga Regency. The purpose of this study was to know the relationship between GSI with a certain eel body length, as well as the gonad histology picture of rice eels in each fishing month. The results of the study as many as 54 eel catches. Ricefield eel was caught in May, June, and August 2019. Eel body length ranges from 16-35 cm with a GSI value of 0.32 – 10.36 %. There is a correlation between eels' body length toward GSI with the value p < 0.01, which was tested using Pearson correlation in the SPSS application. Based on observations using a microscope, many of the eels produced females with a body length ranges of 19-32 cm.
DIFERENSIAL LEUKOSIT IKAN GURAMI (Osphronemus gouramy Lac.) DENGAN PERBEDAAN LEVEL SUPLEMENTASI Spirulina platensis DALAM PAKAN Hikmah Widyaningrum; Sorta Basar Ida Simanjutak; Priyo Susatyo
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (710.971 KB) | DOI: 10.20884/1.sb.2017.4.1.383

Abstract

The aim of this research was to know the effect of supplemented Spirulina platensis in diet on leukocytes differential of gourami (Osphronemous gouramy Lac.) and to obtain the best dose. This research was carried out experimentally using a completely randomized design (RAL), which consists of 4 treatments and 3 repetitions. The fish fed with diets which contain different levels of (0, 2.4, and 6g/kg) Spirulina platensis were investigated. Sampling was done 4 times at intervals of 2 weeks of the feeding experiment, leukocyte differential (monocyte, lymphocyte, neutrophil, eosinophyl) of blood taken from fish were determined. The results showed that the highest percentage of lymphocytes was found in level of supplementation of 4 g/kg of diets, whereas the highest percentage of monocytes, neutrophils and eosinophils were found at the level suplemetation 2 g/kg of diets. Level supplementation of S. platensis 4 g/kg of diets is an efficient and effective level in stimulating immune system cells of gourami, particularly lymphocytes cell.
Induksi Pematangan Gonad dengan Ovaprim-C: untuk Penyediaan Benih Belut Sawah (Monopterus albus) Berkelanjutan Priyo Susatyo; Sugiharto Sugiharto; Elly Tuti Winarni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 3 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.3.247

Abstract

Rice eel (Monopterus albus Zuiew), have economical value is high in Banyumas, national to international markets, but rarely is grown intensively, the seed is still dependent on the catch. Reproduction of swamp eel unlike other fish species in the presence of phase teleostei  intersex / hermafroditus and in nature only spawn once a year. This study will seek to accelerate the scale seed production with natural canvas with several techniques triggers lust and sex organ maturation eels based preparedness aspects of reproductive anatomy, hormones estradiol and testosterone profiles and the intrinsic need for hormonal regulation and the mechanism for the reproductive cycle of the eel rice. In the first year aims to determine (1) the profile of estradiol and testosterone hormonal parent during the natural reproductive cycle, (2) histologic gonadogenesis (development of the testes and ovaries) eel rice during the natural reproductive cycle was evaluated by measuring fecundity. Results: In the control group, body weight gradually decreased during the study. Unlike the control group, the treatment groups were observed every two weeks showed an increase in body weight; Results of the study, in the control group (A0), the value of female eels GSI average of the first two weeks until the fourth two weeks is 0.67 % and 1.78%. While the treatment group A1 was 1.42% - 4.28% (DM-1 s / d DM-4), for the treatment A2 GSI average value is 2.52% - 7.05%; fecundity eggs increased during induction period; calibration titer two types of hormones in the first two weeks to the fourth two weeks showed improvement when compared with the control group; histological profile of rice eel gonad after induction of ovaprim-C show that up to the fourth two weeks, gonad has reached late-stage yolk globule (advanced primary oocyte), even the most mature oocyte has reached the stage: in male eels up to the fourth two weeks has reached the stage of spermatozoa
Pengujian Skala Karamba Jaring Apung Benih F2 Lukas (Puntius bramoides) Produk Pradomestikasi (I): Kualitas Telur, Sintasan Benih dan Kemampuan Memijah Priyo Susatyo; Sugiharto Sugiharto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.3.306

Abstract

Pre domestication research has been done on fish catches from the Serayu river,  Banyumas, lukas fish (Puntius bramoides) and brek fish (P. orphoides) in natural ponds. The parents as pre domestication product is already successfully demonstrated the ability of adaptive in the new environment in terms of aspects of natural food, has been known to their periodically hormonal profiles, profiles of gametogenesis and spawning capabilities in the new environment (natural pond). Further research aims to determine : (1) The degree of eggs hatching of lukas, (2) survival rate of  F2 seeds and (3) the degree of mortality in the floating net on Serayu river. (4) how long it will take and how the ability of the  post spawning F1 parents to be able to spawn again. Research using survey method. Some units of floating net used for maintenance, testing spawning and growth of parents and seeds of lukas. The results : (1) The degree of eggs hatching between 51%-89%; (2) degree of viability of seeds in floating net  is 54%-92%; (3) while the mortality rate of  seeds 8%-48% and (4) the length of time it takes  male and female parents to reach the next spawning period is ± 6 months. The F1 parents spawn after 6 months on a scale of cages.