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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Scripta Biologica BioEksakta : Jurnal Ilmiah Biologi Unsoed Molekul: Jurnal Ilmiah Kimia
Sorta Basar Ida Simanjuntak
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Pengaruh Pemberian Ekstrak Spirulina platensis terhadap Respon Imun Humoral Mencit setelah Uji Tantang Dengan Takizoit Sorta Basar Ida Simanjuntak; Sukarti Moeljopawiro; Wayan Tunas Artama; Subagus Wahyuono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i1.61

Abstract

Toksoplasmosis adalah penyakit yang disebabkan oleh infeksi Toxoplasma gondii. Penyakit ini sangat berbahaya pada hewan maupun manusia. Toksoplasmosis sampai sekarang masih sulit ditanggulangi. Untuk itu, dicari akternatif dengan cara pemberian imunostimulator, seperti Spirulina platensis. Tujuan penelitian ini adalah untuk mengetahui ekstrak Spirulina platensis yang paling potensial meningkatkan respon imun humoral dan mengetahui dosis Spirulina platensis yang efektif dalam meningkatkan respons imun humoral. Rancangan penelitian adalah Rancangan Acak Lengkap pola Faktorial. Faktor pertama adalah jenis ekstrak (ekstrak etil asetat, ekstrak air dan Spirulina platensis murni). Faktor kedua adalah dosis ekstrak (0, 5, 10, 15 mg/ekor mencit). Hasil penelitian menunjukkan bahwa ekstrak etil asetat dengan dosis 5 mg/ekor mencit adalah yang terbaik meningkatkan respon imun humoral. Uji lanjut dengan Latin Square Design (LSD) menunjukkan bahwa terdapat interaksi antara jenis ekstrak dan dosis ekstrak.
PENGARUH SUPLEMENTASI Chlorella vulgaris PADA PAKAN UNTUK MENINGKATKAN ALBUMIN DALAM SERUM IKAN NILEM (Osteochilus vittatus) Kiki Siti Zakiah; Sorta Basar Ida Simanjuntak; Eko Setio Wibowo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.2144

Abstract

Nilem fish belongs to freshwater fish commodity that has been widely cultivation. Some factors influencing fish cultivation are good quality of feed, quantity, size, and shape. Quality feed works as a primary energy source as well as improves the digestive system of fish, with the expectation that the fish growth and health are optimal. Information concerning health status in cultivation Nilem has been used as a reference for improving cultivation methods and controlling disease attacks. The population and production of fish cultivation is experiencing serious problems due to infection from various types of pathogens. Therefore, a viable alternative is needed to prevent and overcome diseases by utilizing immunostimulant substances derived from synthetic substances that can increase the activity of non-specific immune defenses and stimulate antibiotic-forming organs in fish body. Immunostimulant is a substance that is capable of increasing the mechanism of non-specific immune response of fish one of them is by adding Chlorella vulgaris extract to the feed. This research objective is to determine the influence of Chlorella vulgaris supplementation on total blood albumin in nilem fish. This research was conducted by sampling method consisting of 5 treatments with further 4 replications. The treatments were feed supplementation of 0 g.kg-1, 2 g.kg-1, 3 g.kg-1, 4 g.kg-1, and 6 g.kg-1. Independent and dependent variables were used in this research; the independent variable was the dose of Chlorella vulgaris in the feed and the dependent variable was blood albumin concentration. The measuring of plasma albumin concentration was done using the Dyasis kit method with a spectrophotometer. The obtained data were then analyzed by ANOVA, followed by the Least Significant Difference (LSD) at the 95% confidence level if there is a real difference then followed by Tukey's test at the same confidence level. The results showed that the average value of serum albumin concentration of nilem fish from all treatments was between 4.2761 – 7.1826 g/dL. The normal albumin concentration was found in the treatment 4 g.kg-1 Chlorella vulgaris supplementation which was 4.77 g/dL. Meanwhile, the highest concentration was found from the supplementation of Chlorella vulgaris at 3 g.kg-1 feed which was 6.677 g/dL.
PERBEDAAN DOSIS ALUMINIUM SULFAT (TAWAS) TERHADAP KUALITAS SPERMATOZOA DAN KADAR ALUMINIUM DALAM TESTIS MENCIT (Mus Musculus) SWISS WEBSTER Nuraini Hidayah Khasanah; Gratiana Ekaningsih Wijayanti; Sorta Basar Ida Simanjuntak
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

This study designated to evaluate the quality of reproductive organs system in the form of testicular weight, testicular volume and weight of epididymis; quality of spermatozoa include concentration, viability, motility, and morphology of spermatozoa; and aluminum accumulation in mice testis after administration of different aluminum sulfate dose, i.e., 0, 122,5, 245 and 490 mg/kg bb for 45 days were orally administrated using feeding tube through oral route for 45 days. The next objective is to evaluate the correlation between the doses of aluminum sulfate with each variable, as well as to assess the most influential doses of aluminum sulfate in reducing testicular weight and volume, epididymal weight, concentration, viability, motility, and morphology of cauda epididymal spermatozoa and increasing aluminum levels in testes Mice. The results showed that aluminum sulfate did not significantly (p>0.05) decrease testicular weight, testicular volume, epididymal weight, concentration and viability of spermatozoa, but significantly (p<0.05) decreased spermatozoa motility at doses of 245 and 490 mg/kg bb and reduce the proportion of spermatozoa with normal morphology at 490 mg/kg BW. The decrease of motility and percentage of spermatozoa with normal morphology is inversely proportional to the dose of aluminum sulfate each with the value of r = -0.034 and r = -457. Administration of aluminum sulfate for 45 days did not increase aluminum levels in the testes of mice. The use of aluminum sulfate in food processing or drinking water is still safe for human reproductive health.
Peran Hormon Kortisol dalam Osmoregulasi Ikan Sidat, Anguilla bicolor, pada Lingkungan Bersalinitas Untung Susilo; Farida Nur Rachmawati; Sorta Basar Ida Simanjuntak
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.3.281

Abstract

The osmoregulatory capacity of Anguilla bicolor in the sea water is influenced by hormonal activities.  Therefore, the aim of this study was to know the influence of cortisol on osmoregulation of the eel at the different levels of salinity medium. An experimental method with six treatments on randomized completely design was used in this study. The treatments were (1) fish acclimated at water salinity 15 ppt without hormone injection; (2) fish acclimated at water salinity of 30 ppt without hormone injection; (3) fish acclimated at water salinity of 15 ppt and injected with 4 µg cortisol/gr body weight; (4) fish acclimated at water salinity 15 ppt and injected with 8 µg cortisol/gr body weight; (5) fish aclimated at water salinity of 30 ppt and injected with 4 µg cortisol/g body weight; (6) fish acclimated at water salinity of 30 ppt and injected with 8 µg cortisol/g body weight. All of the treatments were replicated four times. Data were analyzed using One way ANOVA followed by Least Significant Difference. The results showed that the cortisol has significant effect (P<.05) on plasma osmolality only at the early of acclimation on medium 30 ppt after injection, especially at 6 and 12 hours after cortisol treatment, and there was no significant efffect of cortisol treatment (P>.05) if the acclimation increased. Osmoregulatory capacity on medium of 15 ppt and 30 ppt were influenced by cortisol treatment at 6 hours acclimation (P<.05), but only that aclimated in medium opf 5 ppt was influenced by cortisol treatment at 12 hours acclimation. Increased acclimation on medium of 15 ppt and 30 ppt was not influenced by cortisol treatment (P>.05). Water body content was not influenced by cortisol treatment (P>.05) at all medium acclimation. At six hour acclimation, treatment cortisol has significant difference (P<.05) on hematocrite only at medium 30 ppt, but cortisol treatment has significant difference (P<.05) at medium 15 ppt and 30 ppt in 12 hours acclimation. Increased acclimation has no significant difference (P>.05) on treatment cortisol. Cortisol injection has no significant difference (P>.05) on plasma glucose on all medium and only on 7 days aclimation, the plasma glucose has significant difference (P<.05) after injected by cortisol. It could be concluded that cortisol treatment has a role on eel osmoregulation at early acclimation. Haematocrite account was also influenced by cortisol injection, but only at the early acclimation. Water body content, blood glucose and total body energy were not influenced by cortisol.
Lipase Activity, Hematological and Blood Biochemistry of Osphronemus gouramy Fed with Suplementation of Spirulina platensis and Chlorella vulgaris Sorta Basar Ida Simanjuntak; Hana Hana; Riska Yunida; Maditaningtyas Hawwa Zuwanda
Molekul Vol 17 No 1 (2022)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2022.17.1.5602

Abstract

Spirulina platensis and Chlorella vulgaris are algae that contain high nutrients, such as protein, vitamins, and minerals. The purpose of this study was to determine the lipase activity, hematological, blood biochemistry and to obtain the most effective supplementation of Spirulina platensis and Chlorella vulgaris in feed to enhance lipase activity, hematological parameter, blood biochemistry of gourami. The study was conducted experimentally in which 100 gourami fish were assigned randomely to the following treatments: P1 = Spirulina platensis 6 g kg-1 feed; P2 = Chlorella vulgaris 4 g kg-1; P3 = Spirulina platensis 3 g kg-1 + Chlorella vulgaris 2 g kg-1; P4 = Spirulina platensis 2 g kg-1 + Chlorella vulgaris 3 g kg-1; and C = feed without supplementation as control, in four replicates. Lipase activity was measured in various digestive organs at pH 2, 5, 7, 8 and 10. The results showed that supplementation of Spirulina platensis and Chlorella vulgaris affected lipase activity; hematological parameter and blood biochemistry of gourami. The combination Spirulina platensis + Chlorella vulgaris supplementation in feed showed the highest increased of the lipase activity, hematological parameter and blood biochemistry of gourami. Spirulina platensis + Chlorella vulgaris supplementation in feed might improve growth and immunity since the increase of digestive enzyme functioning which enhances feed utilization and the increase of biochemical parameters of blood, respectively.
Co-Authors Eko Setio Wibowo Farida Nur Rachmawati Gratiana Ekaningsih Wijayanti Hana Hana Kiki Siti Zakiah Maditaningtyas Hawwa Zuwanda Nuraini Hidayah Khasanah Riska Yunida Subagus Wahyuono Sukarti Moeljopawiro Untung Susilo Wayan Tunas Artama