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AMARILA MALIK
UNIVERSITAS INDONESIA
Medicine & Pharmacology

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Isolasi dan Karakterisasi Protease Serupa Tripsin (PST) dari Lactobacillus plantarum FNCC 0270 TRISMILAH TRISMILAH; WAHONO SUMARYONO; AMARILA MALIK; MOHAMAD SADIKIN
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 1 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Trypsin is an enzyme which is important for research as well as for the pharmaceutical industry, medicine and health, which is especially developed as a raw material for digestive enzymes. This study aimed to characterize PST from Lactobacillus plantarum FNCC 0270 that have not been studied as a producer of trypsin. Fermentation for producing PST by L . plantarum FNCC 0270 was performed in the LKB fermentor with working volume of 3.5 liters, at temperature of 37 oC, pH value of 8, agitation rate of 77 rpm and aeration of 0.5 vvm. The media composition were 3.64% baker’s yeast, 1.21% glucose, 0.13% skim milk. The fementation resulted in enzyme activities of 0.00129 U/mL and protein content of 0.49 mg/mL. Purification of the PST conducted by ultrafiltration Hollow Fiber Catridge 5 kD, presipitated with ammonium sulfate saturated (30-70%), 30 kD membrane polyesthersulfone, ion exchange column chromatography Q-XL and HiTrap affinity column chromatography. All of system increased purity of the crude PST. SDS-PAGE of PST indicated the molecular weights of 47.35; 38.42;21.40 and 12.96 kD. The PST was stable at pH value of 8 and temperature range of 25-35 oC. It is proved that the same half-life was 693.2 minutes. Km and Vmax values of the PST were 0.231 mM and 0.001 U/mL, respectively using N-α-benzoyl-DL-arginine-p-nitroanilide as the substrate. PST was inhibited by EDTA, Ca2+, Zn2+, Mg2+, Mn2+ and specific substrates. Based on the result of characterization of PST from L. plantarum FNCC 0270 can be concluded that the trypsin–like protease (PST) was trypsin.
Pengkulturan Sampel Susu Fermentasi yang Mengandung Probiotik dan Identilikasinya dengan PCR AMARILA MALIK; INDRIANI INDRIANI; LILIANA SARAGIH; MAKSUM RADJI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 1 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Lactic Acid Bacteria (LAB) has long been used in fermented milk because its stability, unique taste and texture and also for its health benents that are provided by the probiotic. There are many fermented dairy products in the market nowadays that declared containing living culture or as probiotics. The aim of this study was to identify LAB from fermented milk product by Visual inspection on plate culture for their morphology, as well by molecular technique performing PCR and DNA sequencing methods. Morphology and growth incubation time of each isolate was observed. A pair of primer that is highly speciiic for LAB targeting the gene encoding for 168 rRNA, which represents sequences of region 677 to 693 of E. coli numbering has been used for PCR identiiication in this study. A total of nineteen isolates were obtained from 10 fermented milk samples that were grown on de Mann Rogosa Sharpe Agar (MRSA) media. Nine out of 19 isolates which represent each of the samples were selected. Genomic DNA extracted from those 9 isolates were used as template for PCR. The results showed that 8 amplicons were positively identitied as LAB, but only three identities indicated similar species as declared, while 5 others did not.
Respon Galur Leuconostoc mesenteroides dan Weissella confusa terhadap pH Kondisi Pertumbuhan Menggunakan Antibiotik sebagai Indikator AMARILA MALIK; DEWI PUSPITA; RIZKI AYU AMALIA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Lactic acid bacteria is known to contribute to human health as exopolysaccharide producer, bacteriocin producer, live vaccins, starter in fermentation process and as probiotics. This study aimed to observe Leuconostoc mesenteroides and Weisella confusa strains response under manipulated growth condition for carbon source composition and on pH as a model, using various antibiotics as indicator. MRS and modified MRS (50% normal dextrose concentration) were used. Modification of pH representing acidic and alkaline condition were 4.6 and 9.0. Disc diffusion method was employed using six different antibiotics. Result showed that all strains grown in acidic standard MRS tend to be more sensitive to amoxicillin (Am), chloramphenicol (Ch) and polymyxin B (PoB), but more resistant to ciprofloxacin (Ci), while in the acidic modified MRS, the strains respond more sensitive to Am, Ch, Ci and PoB. When grown in alkaline standard MRS, all strains respond more sensitive to Am, Ci and PoB, but more resistant to Ch, while in alkaline modified MRS, they respond more resistant to Am and Ch but more sensitive to Ci and PoB. Most of the strains did not show inhibition against vancomycin and none of strains showed inhibition against sulphamethoxazole-trimethoprim under conditions applied.
Skrining Bakteriosin dari beberapa Galur Bakteri Asam Laktat Isolat Lokal Genus Streptococcus dan Weissella RAFIKA SARI; CESILIA ANITA; MAKSUM RADJI; AMARILA MALIK
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 2 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Many lactic acid bacteria (LAB) are known to produce antimicrobial peptides, termed bacteriocins. Bacteriocins are ribosomally synthesized antimicrobial peptides which kill closely related bacteria. Bacteria producing bacteriocin are also known to produce bacteriocin immunity protein (bip). This study aimed to identify bacteriocins activity in an attempt to isolate the bacteriocin and the bip. In this study, genus Streptococcus and Weissella from our LAB collection were screened on well diffusion agar for antagonism assay. Growth inhibition zone observation has been carried out by antagonism assay using Leu. mesenteroides TISTR 120, Leu. mesenteroides JCM 6124, Staphylococcus aureus FNCC 0047, Bacillus subtilis FNCC 0061, Escherichia coli FNCC 0183, Salmonella typhi FNCC 0165, Pseudomonas aeruginosa FNCC 0063, and Listeria monocytogenes FNCC 0156 as indicator strains. Protease K, catalase, and trypsin were also used following the first screening assay for confirmation test. Results showed that both Strep. macedonicus MBF10-2 and W. confusa MBF8-1 posses bacteriocin activity although both against Leu. mesenteroides only, the TISTR 120 and JCM 6124 indicator strains
Co-Authors CESILIA ANITA Dewi Puspita INDRIANI INDRIANI LILIANA SARAGIH Maksum Radji Mohamad Sadikin RAFIKA SARI RIZKI AYU AMALIA TRISMILAH TRISMILAH Wahono Sumaryono