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All Journal Jurnal Medik Veteriner
Suwarno Suwarno
Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga
Biochemistry, Genetics & Molecular Biology Veterinary

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Serodeteksi Brucellosis dengan Metode Rose Bengal Test dan Complement Fixation Test pada Sapi Perah di Banyuwangi Estiary Wilujeng; Suwarno Suwarno; Ratih Novita Praja; Iwan Sahrial Hamid; Maya Nurwartanti Yunita; Prima Ayu Wibawati
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.188-195

Abstract

Tujuan penelitian adalah untuk mendeteksi adanya antibodi dari Brucella pada ternak sapi perah betina di Banyuwangi menggunakan uji Rose Bengal Test (RBT) untuk uji cepat dan dikonfirmasi dengan menggunakan Complement Fixation Test (CFT). Sebanyak 84 sampel dari 516 sapi perah betina di Banyuwangi, kemudian diambil secara acak. Sampel yang diambil berasal dari serum sapi perah betina. Hasil penelitian ditemukan dua sampel yang dinyatakan positif Brucellosis dengan uji RBT dan CFT. RBT merupakan uji screening yang dapat memberikan hasil positif palsu, dan kemudian dikonfirmasi dengan uji CFT untuk melihat titer hasil vaksin atau infeksi alam. Hasil positif pada uji RBT memiliki hubungan dengan hasil titer CFT karena titer antibodi pada RBT dapat diukur dari derajat aglutinasi yang terbentuk. Hasil seropositif penyakit Brucellosis di Banyuwangi sebesar 2%.
Detection of Brucellosis in Dairy Cattle in Turen District Malang Regency using Rose Bengal Test (RBT) and Complement Fixation Test (CFT) Methods Imbi Kusumastuti; Wiwiek Tyasningsih; Ratih Novita Praja; Suwarno Suwarno; Maya Nurwartanti Yunita; Aditya Yudhana
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.42-47

Abstract

The purpose of this research was to detect Brucellosis in female dairy cattle in Turen District, Malang Regency. A total of 78 samples were used in this research taken from 351 total population of female dairy cattle that have been calculated based on the slovin formula. The methods used in this research were Rose Bengal Test (RBT) for the screening test and continued with Complement Fixation Test (CFT) for the confirmation test. The results RBT of this research clearly showed that positive results from 10 samples were confirmed as a final diagnostic test of Brucellosis using CFT. CFT showed a positive result from 9 samples and negative in 1 sample. The percentage of Brucellosis occurrence in the Turen District was recorded at 11.5%. Moreover, Turen District also became an area with a high Brucellosis occurrence (>2%).
Co-Authors Aditya Yudhana Estiary Wilujeng Imbi Kusumastuti Iwan Sahrial Hamid Maya Nurwartanti Yunita Prima Ayu Wibawati Ratih Novita Praja Tyasningsih, Wiwiek