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All Journal Jurnal Medik Veteriner ARSHI Veterinary Letters Veterinary Biomedical and Clinical Journal
Indah Amalia Amri
Fakultas Kedokteran Hewan, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary

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Toksisitas Larutan Perak Nitrat (AgNO3) pada Mencit Balb-c Berdasarkan Kadar SGPT dan SGOT Indah Amalia Amri; Muhamad Ferian Hendrasmara; Dahliatul Qosimah; Ajeng Aeka; Nofan Rickyawan; Wawid Purwatiningsih; Fidi Nur Aini Eka Puji Dameanti
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.251-257

Abstract

Perak Nitrat (AgNO3) sering dimanfaatkan di bidang kesehatan sebagai bahan antimikroba. Efek pada organisme yang terpapar perak nitrat dapat mengakibatkan adanya akumulasi toksik pada hepar. Penelitian ini bertujuan mengetahui dampak toksisitas perak nitrat terhadap kadar SGPT dan SGOT pada mencit Balb-c. Hewan coba yang digunakan pada penelitian ini berjumlah 20 ekor mencit Balb-c dengan berat badan 25-30  gram dan umur 2-3 bulan serta masa pemberian perlakuan selama 14 hari. Perlakuan yang diberikan terdiri dari kelompok K- (tanpa induksi), P1 (100 µg/ml), P2 (200 µg/ml), P3 (300 µg/ml), P4 (400 µg/ml). Kadar SGPT dan SGOT diamati dengan menggunakan metode spektrofotometri. Data dianalisa menggunakan uji One Way ANOVA dengan tingkat kepercayaan 95%. Hasil dan kesimpulan menunjukkan induksi larutan perak nitrat pada mencit Balb-c dengan dosis P4 (400 µg/ml) dan volume induksi 0.5 ml dapat menyebabkan toksisitas pada mencit yang ditandai dengan peningkatan kadar SGPT dan SGOT.
Peneguhan diagnosa scabiosis metode sitologi kulit pada kucing domestik di Kota Malang Dodik Prasetyo; Indah Amalia Amri; Sri Murwani; Dahliatul Qosimah
ARSHI Veterinary Letters Vol. 3 No. 2 (2019): ARSHI Veterinary Letters - Mei 2019
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.3.2.27-28

Abstract

Hewan harus terbebas dari rasa sakit dan penyakit, merupakan salah satu aspek dari “Five Freedom of Life”. Kucing domestik liar banyak dijumpai di kota Malang, baik di jalanan dan pasar yang diduga terkena Scabiosis. Kegiatan pengabdian masyarakat ini diharapkan dapat meningkatkan kesadaran, kepedulian, dan pengetahuan masyarakat terhadap kesehatan hewan melalui pelayanan pemeriksaan fisik kesehatan dan pemeriksaan penunjang dengan metode sitologi kulit. Teknik scrapping kulit digunakan untuk peneguhan diagnosa Scabiosis sebagai dasar untuk penanganan dan pengobatan. Kucing yang positif menderita scabiosis dilakukan tindakan kuratif, preventif dan pemberian supportif. Hasil sitologi pada 85 ekor kucing menunjukkan sebanyak 38 ekor kucing liar positif ditemukan parasit dan 47 lainnya tidak ditemukan parasit.
Effect of Oregano (Origanum vulgare) Extract on Kidney Histomorphometry and Histopathology in Mice (Mus musculus) Colibacillosis Model: Pengaruh Pemberian Ekstrak Oregano (Origanum vulgare) terhadap Histomorfometri dan Histopatologi Ginjal pada Mencit (Mus musculus) Model Kolibasilosis Bian Febry Rohmana Alviano; Indah Amalia Amri
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.4

Abstract

Colibacillosis is a disease that infects the digestive tract due to infection with the pathogen Escherichia coli. E. coli is able to cause bacteremia so that E. coli can reach the kidney through blood flowing to the kidney. To reduce the side effects of treatment using antibiotics, the herbal medicine oregano (Origanum vulgare) which contains phenols (carvacrol and thymol) works as an antibacterial. The benefits of this research are as a source of information and the basis for further research related to the use of oregano extract in the treatment of colibacillosis due to E. coli infection. The study used 20 male mice, divided into 5 applicable groups, namely K-, healthy mice were not given any treatment; K+ was induced by Streptomycin 20 mg and infected with E.coli 3 x 109 CFU/ml, 0.5 ml; P1 was induced by Streptomycin 20 mg and infected with E.coli 3 x 109 CFU/ml, 0.5 ml + Origanum vulgare extract 25 mg/kg; P2 was induced by Streptomycin 20 mg and infected with Escherichia coli 3 x 109 CFU/ml, 0.5ml + Origanum vulgare extract 50mg/kg; P3 was induced by Streptomycin 20 mg and infected with E.coli 3 x 109 CFU/ml, 0.5 ml + Origanum vulgare extract 100 mg/kg. Kidney was taken for histopathological preparations with hematoxylin eosin (HE) staining. After that, it was analyzed using Kruskal Wallis and continued with Mann Whitney. Histomorphometry semiquantitative measures the width of the Bowman's glomerular capsule and the length of the tubular lumen and then analyzed using One Way Analysis of Variance (ANOVA). The results of this study showed that oregano extract had an effect on histomorphometry and histopathology of renal, with an optimal dose is 5 mg/mice.
Efficacy of Oregano Extract Ointment on Fibroblast Cells and Epidermis in Albino Rats with Excisional Wound Model Muhammad Arfan Lesmana; Indah Amalia Amri; Ardilla Rachma Cahyanissa; Kartika Myrnandari Geoputri; Agri Kaltaria Anisa; Andreas Bandang Hardian
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.262-270

Abstract

This study aimed to determine the effect of oregano extract ointment therapy on the number of fibroblast cells and the epidermal thickness in excised wounds. The experimental animals used were 20 male Wistar strain albino rats, 12 weeks old, weighing 100–150 g, divided into 5 treatment groups i.e., (C-) not excised and without ointment, (C+) excised and without ointment, and the treatment group were excised using a 5 x 5 mm biopsy punch and treated with oregano extract ointment using concentrations of (T1) 3%, (T2) 6% and (T3) 9% twice daily for 14 days in an excised wound. The variables observed in this study were the number of fibroblast cells and the epidermal thickness by the HE staining method then measured using ImageJ. Data analysis used the One-Way ANOVA test followed by the Tukey test (p < 0,05). The results showed a decrease in the number of fibroblast cells and an increase in the epidermal thickness in the 6% concentration ointment. This study concluded that the administration of oregano extract ointment with a concentration of 6% was the optimal concentration in accelerating the final proliferative phase, characterized by a decrease in fibroblast cells and an increase in epidermal thickness.
Identification of Colibacillosis and Antibiotic Resistance Test in Free-range Chicken: Identifikasi Kolibasilosis dan Uji Resistensi Antibiotik pada Ayam Kampung Eka Wulandari; Indah Amalia Amri
Veterinary Biomedical and Clinical Journal Vol. 4 No. 1 (2022)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2022.004.01.4

Abstract

Escherichia coli are normal flora in the digestive tract of animals and humans but can be opportunistic under certain conditions. Apart from normal flora, there are also pathogenic strains of the E. coli bacteria that can cause disease in the hospes. The disease that can be caused by E. coli bacteria is colibacillosis which often occurs in poultry. One of the clinical symptoms that appear in chickens suffering from colibasillosis is diarrhea which can arise due to enteritis. This study aims to identify the incidence of colibacillosis and antibiotic resistance of E. coli bacteria in Free-range Chicken. Clinical symptoms showed that the animal looked weak, had diarrhea, and had feces around the cloaca. The necropsy results showed the presence of hemorrhage in the duodenum and caecum. Samples of organ swabs that experienced hemorrhage were then tested for isolation and identification of E.coli bacteria and antibiotic resistance using the Kirby-Bauer method for five types of antibiotics, penicillin, bacitracin, gentamicin, cefadroxil, and ciprofloxacin. The test results showed that the Free-range Chicken had colibacillosis. The E. coli bacteria that cause colibacillosis were resistant to penicillin and bacitracin antibiotics, intermediates to cefadroxil, and sensitive to ciprofloxacin and gentamicin.
Co-Authors Agri Kaltaria Anisa Ajeng Aeka Ardilla Rachma Cahyanissa Bian Febry Rohmana Alviano Dameanti, Fidi Nur Aini Eka Puji Dodik Prasetyo Eka Wulandari Hardian, Andreas Bandang Kartika Myrnandari Geoputri Muhamad Ferian Hendrasmara Muhammad Arfan Lesmana Nofan Rickyawan Qosimah, Dahliatul Wawid Purwatiningsih