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All Journal Borneo Journal of Pharmacy
Alfat Fadri
Sekolah Tinggi Ilmu Farmasi Makassar
Medicine & Pharmacology

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Cytotoxic Effect of the Paku Atai Merah (Angiopteris ferox Copel) Fraction on MCF-7 and HeLa Cells and its Compound Profile by GC-MS Syamsu Nur; Andi Nur Aisyah; Endang Lukitaningsih; Rumiyati Rumiyati; Alfat Fadri; Marwati Marwati
Borneo Journal of Pharmacy Vol. 5 No. 1 (2022): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v5i1.2943

Abstract

Cancer is a condition of abnormal cell proliferation of tissue cells in the body that becomes malignant. It can attack other parts of the body and affect the normal function of the body organs. The sample used in this study was tubers of paku atai merah (Angiopteris ferox Copel), then extracted using 96% ethanol eluent to obtain a thick extract. The ethanolic extract of A. ferox was fractionated using column chromatography to get the active fraction to characterize the compound using thin-layer chromatography and gas chromatography-mass spectroscopy (GC-MS) and tested its cytotoxic effectiveness on MCF-7 and HeLa cancer cells. The results of this study were obtained from fractionation using the column chromatography method to get sub-fraction C and the results of compound characterization using GC-MS and obtained variations in the class of compounds contained in the sample: amino acids, glucosinolates, alkaloids, flavonoids, and terpenoids. Based on the cytotoxic effect test of sub-fraction C on MCF-7 cells, the results obtained moderate cytotoxic effects with an IC50 value of 61.027 µg/mL, and HeLa cells had an IC50 value of 521.03 µg/mL, which was categorized as having a weak cytotoxic effect. Based on the results obtained from this study, it can be concluded that sub-fraction C of A. ferox tubers has a cytotoxic effect on MCF-7 cells to be used as a reference for tracing pure compounds from A. ferox tuber.
Phenolic and Flavonoid Content of Black Mulberry (Morus nigra L.) Stem and Their Evaluation Antioxidant and Cytotoxic Profile Syamsu Nur; Fitriyanti Jumaetri Sami; Marwati Marwati; Nursamsiar Nursamsiar; Alfat Fadri; Khairuddin Khairuddin
Borneo Journal of Pharmacy Vol. 5 No. 4 (2022): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v5i4.3760

Abstract

The black mulberry (Morus nigra) plant has been widely developed for its bioactivity as natural medicine. This study evaluated plant extracts' total phenolic and flavonoid content and their correlation to M. nigra stem's antioxidant activity and toxicity. Dry powder from the M. nigra stem was extracted by maceration with 96% ethanol to obtain a thick extract (TE) and fractionated using hexane (HF), ethyl acetate (EAF), and ethanol-aqueous (EF). The samples were analyzed for a compound using reagents and the determination of total phenolic and flavonoid content. The samples were evaluated for antioxidant activity using several parameters and their cytotoxic effects using the BSLT method. Identification of compounds in EAF was confirmed to contain phenolic, flavonoids, alkaloids, saponins, tannins, steroids, and terpenoids. EAF showed higher phenolic and flavonoid content than others. The evaluation of antioxidant activity showed that extracts and fractions from M. nigra stems showed the ability to reduce ions and free radicals. EF sample has activity in reducing Mo (IV) ion by TAC method of 98.82±0.53 µM/mg, indicating substantial antioxidant capacity. In addition, EAF samples showed potential activity in reducing DPPH, hydroxyl, and peroxide radicals in the β-carotene bleaching method with IC50 values of 12.13, 42.06, and 57.6 µg/mL, respectively. Similar activity was also seen in the cytotoxic effect of a robust EAF sample with an LC50 value of 16.31 µg/mL. The results show that EAF can be developed as a raw material for traditional medicine as an antioxidant and anticancer candidate with a significant flavonoid and phenolics content.
Co-Authors Ali Djamhuri Andi Nur Aisyah Endang Lukitaningsih Endang Lukitaningsih Fitriyanti Jumaetri Sami Khairuddin Khairuddin Marwati Marwati Nursamsiar Nursamsiar Rumiyati Rumiyati Syamsu Nur