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Identifikasi Keragaman Gen Kalpastatin (CAST) pada Ayam Lokal Indonesia (IDENTIFICATION OF POLYMORPHISM CALPASTATINE GENE IN LOCAL CHICKEN) Ahmad Saleh Harahap; Cece Sumantri; Niken Ulupi; Sri Darwati; Tike Sartika
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Calpastatin (CAST) gene is one of the genes that play a role in the process of tenderization of meat. CAST gene serves as an inhibitor of meat tenderness. The purpose of this study was to identify Single Nucleotide Polymorphisms (SNP) in the CAST gene of kampong chickens. A total of 61 kampong chickens used were used in the study including strain cobb, F1 of strain cobb-kampong, merawang, sentul, nunukan, and pelung, respectively. The method used is extracted DNA from blood samples, then amplified by Polymerase Chain Reaction (PCR) and then genotyping by DNA sequencing. The results showed that SNP was identified at position g.42988G> T in the intron 11 CAST gene of merawang chicken with genotype GG and GT. SNP was not identified in kampong chicken, strain cobb, F1 of strain cobb-kampong, sentul, nunukan, and pelung chicken, respectively. In conclusion, the intron 11 CAST gene region with 482 of product length of merawang chicken is polymorphic, whereas the other chicken species is monomorphic. ABSTRAK Gen Calpastatin (CAST) merupakan salah satu gen yang berperan dalam proses keempukan pada daging. Gen CAST berfungsi sebagai inhibitor (penghambat) keempukan daging. Tujuan dari penelitian ini adalah mengidentifikasi Single Nucleotide Polymorphisms (SNP) gen CAST pada ayam kampung. Jumlah ayam yang digunakan 61 ekor ayam kampung, ayam strain cobb, F1 ayam strain cobb-kampung, ayam merawang, ayam sentul, ayam nunukan, dan ayam pelung. Metode yang dilakukan adalah ekstrasi DNA dari sampel darah, kemudian dilakukan amplifikasi menggunakan mesin Polymerase Chain Reaction (PCR) dan selanjutnya dilakukan genotiping melalui DNA sequencing. Hasil penelitian menunjukkan bahwa gen CAST intron 11 pada ayam pengamatan ditemukan SNP posisi g.42988G>T pada ayam merawang dengan genotip GG dan GT, sedangkan pada ayam kampung, ayam strain cobb, F1 ayam kampung dengan strain cobb, ayam sentul, ayam nunukan, ayam merawang dan ayam pelung tidak ditemukan SNP. Simpulan dari penelitian ini memperlihatkan gen CAST daerah intron 11 dengan panjang produk 482 pada ayam merawang bersifat polimorfik, sedangkan jenis ayam yang lain bersifat monomorfik.

Performa Produksi Telur pada Ayam Ketarras Generasi Kedua dengan Bobot Badan yang Berbeda Ahmad Saleh Harahap; Kususiyah Kususiyah; Amir Husaini Karim Amrullah
Buletin Peternakan Tropis Vol 2, No 1 (2021)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/bpt.2.1.48-52

Ayam ketarras merupakan ayam persilangan antara ayam arab dan ayam ras petelur dengan persentase darah 75% ayam arab dan 25% ayam ras petelur. Saat ini ayam ketarras keturunannya sudah pada generasi kedua (G2). Penelitian ini bertujuan untuk melihat performa produksi telur pada ayam ketarras dengan bobot badan yang berbeda. Penelitian ini menggunakan 30 ekor ayam betina Ketarras dengan jenis kelamin betina. Penelitian dilaksanakan di Commercial Zone and Animal Laboratory (CZAL) Jurusan Peternakan, Fakultas Pertanian, Universitas Bengkulu. Kandang yang digunakan adalah kandang batteray yang merupakan kandang individu. Pada saat pemeliharan pemberikan pakan dengan komponen konsentrat khusus ayam petelur, dedak dan jagung giling. Paramater yang diambil ketika penelitian adalah produksi telur, berat telur dan persentase produksi. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap. Data yang diperoleh disajikan berdasarkan rataan dan standar deviasi. Jumlah telur yang dihasilkan dari 30 ekor selama 3 bulan pengamatan sebesar 1.728 butir dengan rataan produksi telur perbulan per ekor adalah 19 butir/ekor/bulan. Secara keseluruhan berat telur yang diperoleh sebesar 48.22 gram. Sementara persentase produksi telur secara keseluruhan yang diperoleh mencapai 63.30 %. Hasil penelitian ini dapat disimpulkan bahwa ayam Ketarras generasi ke-2 umur 11-13 bulan memiliki performa produksi, persentase produksi dan berat telur yang masih bervariasi.

Peningkatan Kapasitas Kelompok Wanita Tani Desa dalam Pemanfaatan Limbah Sawit Sebagai Silase Gustria Ernis; Nola Windirah; Ahmad Saleh Harahap
Berdikari: Jurnal Inovasi dan Penerapan Ipteks Vol 9, No 1 (2021): February
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/berdikari.v9i1.10897

Massive land conversion into palm oil tree plantation causes massive plantation waste (palm fronds) and the decrease of animal food in Jenggalu Village, Sukaraja, Seluma, Bengkulu. The solution for the problem is by utilizing palm fronds as silage (fermented animal food). The purpose of this community service is to improve the ability of female farmer group (Kelompok Wanita Tani/ KWT) Tanjung Aur in utilizing palm fronds as silage. The method applied is theory and practice on how to create silage, preparing the procedure to create silage along with the main ingredients, and then holding the workshop on processing food fermentation from palm fronds using EM-4 activator. To maintain the continuity of the program, besides choosing the person in charge of the equipment, a supervision was also done in the form of monitoring and evaluation. After the fermentation process is completely applied for the livestock, the obtained results indicate that cows really like the food, this can be seen from the consumption increase. From the observation, the animal food and its technology are categorized as ‘useful’ and ‘very useful’ based on KWT members’ understanding. From the activity, it can be concluded that the KWT members’ ability towards the technology of utilizing palm fronds as silage is improved and it will be continuously applied.

Analisis Keuntungan Usaha Penangkaran Burung Murai Batu (Copsychus malabaricus) Di Kota Bengkulu Heri Dwi Putranto; Yossie Yumiati; Alven Syahril Muslim; Ahmad Saleh Harahap
Wahana Peternakan Vol. 6 No. 2 (2022): Wahana Peternakan
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v6i2.601

Abstract Nowadays, breeding captivity of white-rumped shama (Copsychus malabaricus) becomes a popular business among bird lovers in Bengkulu. It has been directed to create an independent livestock business. This captivity business aims to increase household income and gain profits. Captivity breeding has developed into a promising and potential business. This study aimed to analyze the profits of white-rumped shama breeding captivity business in Bengkulu city. The research was purposively conducted in Bengkulu City. A snowball sampling method was used to determine respondents. Total of 19 respondents were interviewed based on questionairres to determine the characteristics of respondents, total cost, total revenue and R/C ratio. The results showed that the majority of white-rumped shama breeders in Bengkulu city were male (95%) and classified into a productive age group. The average total revenue was Rp 150,744,354/year per respondent. The average of R/C ratio was 2.47, which means that white-rumped shama breeding captivity business in Bengkulu is feasible to be developed. Key words: Bengkulu City, Breeding Captivity, Profit Analyses, White-rumped Shama

Analisis Profitabilitas Usaha Penangkaran Murai Batu di Kota Bengkulu Heri Dwi Putranto; Alven Syahril Muslim; Ahmad Saleh Harahap; Dadang Suherman
Bulletin of Tropical Animal Science Vol. 4 No. 1 (2023)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/bpt.4.1.26-31

The research objective was to analyze the income and profitability of the Murai Batu bird breeding business. The implementation starts from February to March 2019 in the Murai Batu captivity. The object used was the Murai Batu breeder in Bengkulu City, Bengkulu Province. The research method used purposive sampling. Data obtained through interviews. Data was calculated for 1 year regarding technical and financial aspects. Data were analyzed descriptively. The Murai Batu breeding business earned an income of Rp. 532,701.76/pair/breeder/year with an average number of Murai Batu of 7 pairs. Production cost was Rp. 2,957,656.37/pair/breeder/year. The profitability achieved was 18.01%. In conclusion, the Murai Batu captive breeding business was profitable. Key words: Murai Batu, income, profitability ABSTRAK Tujuan penelitian untuk menganalisis pendapatan dan profitabilitas usaha penangkaran burung Murai Batu. Pelaksanaan dimulai bulan Februari hingga Maret 2019 di penangkaran burung Murai Batu. Obyek yang digunakan adalah penangkar burung Murai Batu di Kota Bengkulu, Provinsi Bengkulu. Metode penelitian menggunakan purposive sampling. Data diperoleh melalui wawancara. Data dihitung selama 1 tahun mengenai aspek teknis dan keuangan. Data dianalisis secara secara deskriptif. Usaha penangkaran burung Murai Batu memperoleh pendapatan sebesar Rp. 532.701,76/pasang/penangkar/tahun dengan jumlah burung Murai Batu rata-rata 7 pasang. Biaya produksi sebesar Rp. 2.957.656,37/pasang/penangkar/tahun. Profitabilitas yang dicapai sebesar 18,01 %. Dapat dismpulkan usaha penangkaran Murai Batu adalah profitable Kata kunci: Murai Batu, Pendapatan, Profitabilitas.

Analisis Keuntungan Usaha Penangkaran Burung Murai Batu (Copsychus malabaricus) Di Kota Bengkulu Heri Dwi Putranto; Yossie Yumiati; Alven Syahril Muslim; Ahmad Saleh Harahap
Wahana Peternakan Vol. 6 No. 2 (2022): Wahana Peternakan
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v6i2.601

Abstract Nowadays, breeding captivity of white-rumped shama (Copsychus malabaricus) becomes a popular business among bird lovers in Bengkulu. It has been directed to create an independent livestock business. This captivity business aims to increase household income and gain profits. Captivity breeding has developed into a promisingÂ and potential business. This study aimed to analyze the profits of white-rumped shama breeding captivity business in Bengkulu city. The research was purposively conducted in Bengkulu City. A snowball sampling method was used to determine respondents. Total of 19 respondents were interviewed based onÂ questionairres to determine the characteristics of respondents, total cost, total revenue and R/C ratio. The results showed that the majority of white-rumped shama breeders in Bengkulu city were male (95%) and classified into a productive age group. The average total revenue was Rp 150,744,354/year per respondent. The averageÂ of R/C ratio was 2.47, which means that white-rumped shama breeding captivity business in Bengkulu is feasible to be developed. Key words: Bengkulu City, Breeding Captivity, Profit Analyses, White-rumped Shama

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