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All Journal UNEJ e-Proceeding The Indonesian Biomedical Journal BIOSFER : Jurnal Biologi dan Pendidikan Biologi JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Aditya Kurniawan
Biology Education Study Program, Department Of Mathematic And Nature Education, Faculty Of Teacher Training And Education, Universitas Jember, Jl. Kalimantan Tegalboto No.37, Jember
Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Education Environmental Science Public Health Social Sciences

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WIF1 Qualitative-Methylation from Peripheral Blood Could Not Be Used as Biomarker for The Risk of Nasopharyngeal Carcinoma or Smoking Behavior in Yogyakarta Panel Aditya Kurniawan; Erika Diana Risanti; Saihas Suhda; Hanggoro Tri Rinonce; Ery Kus Dwianingsih; Jajah Fachiroh
The Indonesian Biomedical Journal Vol 11, No 3 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i3.810

Abstract

BACKGROUND: Tobacco smoking plays an important role in nasopharyngeal carcinoma (NPC) risk. The Wingless-related integration site (WNT) Inhibitory Factor 1 (WIF1) as one of the tumor suppressor gene (TSG), functions to control cell proliferation through suppression of WNT signaling pathway. WIF1 methylation is one of epigenetic mechanisms which causes overactivated of WNT pathway in NPC development. WIF1 methylation from buffy coat related to smoking status and NPC risk is not known yet.METHODS: This was a nested case-control study involving 39 newly diagnosed NPC patients and 40 healthy controls. All subjects were enrolled at Dr. Sardjito Hospital, Yogyakarta. Subjects were male, with known smoking status, duration, and quantity of cigarette consumed. Parallel DNA isolated from buffy coat was used for WIF1 DNA methylation analysis, by using methylation-specific PCR (MSP) method. Chi-square analysis was used to determine distribution of differences among groups. Two tailed p-value≤0.05 was considered as statistically significant.RESULTS: No significant difference between WIF1 methylation of cases and controls (p=0.30), nor smoking habit among smokers (p=0.51) and non-smokers (p=0.43).CONCLUSION: WIF1 methylation from buffy coat could not be used as an NPC marker nor as a smoking behavior marker.KEYWORDS: buffy coat, cigarette smoking, methylation, nasopharyngeal carcinoma, WIF1
Pheretima javanica K. Ethanol Extract Granules’ Effects on Eosinophil Level, Immunoglobulin E Level, and Organs Histopathology in Rattus norvegicus B. Dwi Wahyuni; Joko Waluyo; Jekti Prihatin; Fersty Isna Kusumawardani; Aditya Kurniawan
The Indonesian Biomedical Journal Vol 13, No 2 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i2.1388

Abstract

BACKGROUND: Traditional medicine that might be useful to treat typhoid fever is earthworms (Pheretima javanica K.). It is important to know whether any substances contained in Pheretima javanica K. extract are toxic and have pharmacological effects, which can be observed from the eosinophil and immunoglobulin E (IgE) levels in the blood.METHODS: Thirty Rattus norvegicus B. were included in this study and were separated into 5 groups, with 6 rats in each group. T1, T2, T3, and T4 were groups of rats treated with 50, 300, 2000, and 5000 mg/kg BW of Pheretima javanica K. ethanol extract granules (PJEEG), respectively, while the control group was a group of rats treated with 0.36 mg Ciprofloxacin. The eosinophil number and the IgE concentration were detected. Physical observations were also conducted to detect any allergic symptoms, toxicity symptoms, or histopathological changes.RESULTS: Administration of PJEEG significantly increased the number of eosinophil in every group, from 49.82 cells/μL to 259.26 cells/μL on average (p<0.001). PJEEG did not significantly affect IgE levels (p=0.624). The results showed that the administration did not cause any allergic and toxicity symptoms. Histopathological analysis of rats’ kidneys and liver also showed that the administration had no significant effect on histopathological changes.CONCLUSION: The administration of PJEEG does not cause allergic symptoms, toxicity symptoms, and histopathological changes in rats. The administrations also significantly increase eosinophil number in rats’ blood. However, the concentration of IgE in rats’ blood was not significantly changed after administration of PJEEG.KEYWORDS: allergies, typhoid fever, earthworms, Pheretima javanica K., eosinophil, immunoglobulin E
Motivasi Siswa Terhadap Kegiatan Praktikum Pembuatan Preparat Apusan Darah Tingkat SMA Di Kabupaten Jember, Indonesia Aditya Kurniawan; Bea Hana Siswati; Nadyatul Ilma Indah Savira
Biosfer : Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2021): BIOSFER: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Unpas,

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/biosfer.v6i2.4849

Abstract

Pandemi COVID-19 telah melanda Indonesia sejak Maret tahun 2020. Sejak saat itu kegiatan diubah dari luring menjadi daring termasuk kegiatan praktikum. Siswa hanya melihat kegiatan praktikum melalui tayangan video yang telah dibuat oleh guru dan laboran. Di bulan September 2021, angka kasus COVID-19 terus turun. Hal ini menyebabkan sekolah – sekolah mulai untuk melakukan kegiatan belajar mengajar secara tatap muka secara terbatas. Perlu dilakukan pengukuran motivasi siswa saat pertama kali melakukan praktikum luring di sekolah. Metode yang dilakukan saat kegiatan praktikum kepada 11 siswa SMAN Rambipuji Jember adalah Participatory Action Research (PAR) dengan tahapan umum berupa Perencanaan, Kegiatan, Refleksi dan Evaluasi. Tingkat motivasi siswa terhadap pembelajaran Biologi khususnya praktikum pembuatan preparat apusan darah adalah sebesar 86%. Motivasi siswa yang sangat tinggi ini dikarenakan siswa dilibatkan dan terjun langsung dalam proses pembuatan preparat sehingga melatih kecerdasan psikomotorik dan kognitif.
Peningkatan Keterampilan Proses Sains Siswa dalam Pembuatan Preparat Apusan Darah pada Praktikum Biologi SMA di Jember Bea Hana Siswati; Nadyatul Ilma Indah Savira; Aditya Kurniawan
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 6 NOMOR 1 MARET 2022 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.168 KB) | DOI: 10.30595/jppm.v6i1.12067

Abstract

Tujuan dari kegiatan pengabdian kepada masyarakat ini adalah untuk meningkatkan Keterampilan Proses Sains praktikum biologi bagi siswa SMA di Kabupaten Jember. Metode yang dilakukan selama menjalankan kegiatan pengabdian kepada masyarakat adalah Participatory Action Research (PAR) dengan tahapan umum berupa Planning (Perencanaan), Action (kegiatan), Reflection (Refleksi), Evaluation (Evaluasi). Kegiatan pertama yaitu perencanaan yang berisi persiapan dan sosialisasi kegiatan pengabdian, kegiatan kedua yaitu aksi/kegiatan yang dilakukan dengan pemberian materi dan pelatihan pembuatan preparat, kegiata ketiga adalah refleksi yang dilakukan dengan cara melakukan pendampingan kepada siswa dan guru dalam membuat preparat apusan darah dan terakhir adalah evaluasi yang dilakukan dengan cara membahas bagaimana keberlanjutan program bersama sekolah mitra. Keterampilan Proses Sains siswa sebelum pelatihan dilakukan memiliki nilai rata-rata kelas 53 dan setelah pelatihan menjadi 94, dengan demikian ada kenaikan sebesar 77,3%. Hasil dari kegiatan pengabdian ini adalah meningkatnya Keterampilan Proses Sains melalui kegiatan praktikum yang dilakukan oleh siswa SMA. Praktikum tersebut berupa kegiatan pembuatan preparat apusan darah.
DIGITAL MARKETING TRAINING TO INCREASE MSME INCOME IN WATES HAMLET, TEJO VILLAGE, MOJOAGUNG, JOMBANG Sudarti Sudarti; Rif’ati Dina Handayani; Aditya Kurniawan; Nurul Faridah
UNEJ e-Proceeding 2022: E-Prosiding Kolokium Hasil Penelitian dan Pengabdian kepada Masyarakat
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Nur Janah's shop is one of the micro businesses in Wates Hamlet that has felt the impact of Covid-19. Nur Janah's shop is quite large and complete, providing various kinds of household needs, but Nur Janah's shop does not have employees, this is because the daily income is not clear and is not enough to pay employees. Merchandise is piling up, there is no marketing effort and not familiar with online sales because of a lack of knowledge of technology. In addition, this shop is also located quite hidden in the hamlet, with the target market only local villagers. This activity aims to provide digital marketing training for the family of the owner of the Nur Janah Store. The training method is carried out through direct private training for 6 times every Saturday. The results of the activity show that Mrs. Nur Janah and her 2 children are already skilled in digital marketing. The impact of this activity is that there is an optimization on the Tokopedia MSME account of the Nur Janah store in terms of product design and description Keywords: Digital marketing, MSME, Marketplace
Co-Authors Bea Hana Siswati Dwi Wahyuni Dwianingsih, Ery Kus Erika Diana Risanti Fersty Isna Kusumawardani Hanggoro Tri Rinonce Jajah Fachiroh, Jajah Jekti Prihatin Joko Waluyo Nadyatul Ilma Indah Savira Nurul Faridah Rif’ati Dina Handayani Saihas Suhda Sudarti Sudarti