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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Biotechnology The Indonesian Biomedical Journal JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Jajah Fachiroh, Jajah
Department Of Histology And Cell Biology, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada, Jl. Farmako Sekip Utara, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Epidemiologi, Stadium, dan Derajat Diferensiasi Kanker Kepala dan Leher Nelsiani To'Bungan; Siti Hamidatul A'liyah; Nastiti Wijayanti; Jajah Fachiroh
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 1 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i1.566

Abstract

Head and neck cancer is one of the deadly types of cancer in Indonesia. The main cause of this cancer is the consumption of alcohol and cigarettes. Head and neck cancer attacks the lips, mouth, palate, pharynx and larynx. Studies about head and neck cancer have been carried out in westerncountries, while in Indonesia is still limited. Earlier studies in western countries expressed that head and neck cancer is more common in men than women. Purpose of research were to determine the epidemiology of head and neck cancer in Indonesia related to patient ratio of men and women andthe correlation of stage and differentiation level of head and neck cancer. Data were obtained from the Rumah Sakit Kanker Dharmais-Pusat Kanker Nasional Jakarta and then analyzed descriptively. The data were analyzed came from 36 patients with head and neck cancer. The results showed, menwith head and neck cancer as much as 52,77% and females 47,22%. There were 16 cases with stage IV cancer, 9 with stage III, 8 with stage II and 2 with stage I. There were 6 cases of stage IV cancer with better differentiation, and there were 2 cases of stage II cancer with a poor differentiation. Head and neck cancer is more common in men than women. There was no correlation between the degree of differentiation-stage head and neck cancer. It was influenced by immunity of each person.Keywords: differentiation level, epidemiology, head and neck cancer, stage
Tetra‐primer amplification refractory mutation system (ARMS) PCR used to detect 3’UTR rs1948 mutation in CHRNB4 Anggi Laksmita Dewi; Dewi Kartikawati Paramita; Jajah Fachiroh
Indonesian Journal of Biotechnology Vol 27, No 1 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.64933

Abstract

Rs1948 A>G is a single nucleotide variation (SNV) in the 3’‐UTR of CHRNB4. Genotyping the synonymous CHRNB4 rs1948 may be useful in identifying a lung cancer susceptibility gene. The study aimed to develop a simple and easy tetra‐primer amplification refractory mutation system (ARMS PCR) for CHRNB4 rs1948. The following steps were taken to optimize tetra‐primer ARMS PCR: 1) determining the gene sequence and position of a single mutation; 2) developing outer and inner primers; 3) amplification of target gene fragments via PCR using an outer primer; 4) genotyping PCR product using Sanger sequencing; 5) determining the optimal annealing temperature and PCR cycle; 6) determining optimal outer and inner primer ratio; and 7) testing the reproducibility of the PCR program and final validation with Sanger sequencing. Genotype (PCR result) was visualized with 3% agarose gel electrophoresis. Optimum condition was determined as annealing temperature of 64.8 ºC and 35 cycles, outer and inner primer ratio of 1:6, and DNA volume of 3 µL. Sanger sequencing confirmed the results of the tetra‐primer ARMS PCR and it was shown that ARMS PCR was able to identify three different variants of CHRNB4 rs1948.
Review of immune responses correlated with COVID-19 outcomes: the fight, debacle and aftermath in the Indonesian context. Dian Eurike Septyaningtrias; Jajah Fachiroh; Dewi Kartikawati Paramita; Dewajani Purnomosari; Rina Susilowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 52, No 3 (2020): Special Issue: COVID-19
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.092 KB) | DOI: 10.19106/JMedSciSI005203202004

Abstract

In the current pandemic, the highly contagious nature of the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) leads to an enormous burden for the global health care system and creates challenging socioeconomic problems. Respiratory mucosa, the main entrance of SARS-CoV-2 infection, are equipped with an innate immune defense system as the initial response against infection. Activation of the adaptive immune system facilitates viral clearance as well as providing immunological memory for prevention from subsequent exposure. However, despite repeated efforts at implementing appropriate interventions, severe and fatal cases are continuing to occur and reports of recurrent cases need clarification. Host factors may contribute to the severity of the diseases while viral immune evasion is a common phenomenon leading to severe outcomes and recurrent infection. Discussions of immunological-based tests for screening, herd immunity, along with the possible advantages or potentially futile efforts of development of vaccine and alternative immunotherapy have become a part of daily household conversations. In this review, evidence of innate and adaptive immune responses or lack of them, and immunological problems relevant for SARS-CoV-2 will be summarized. Finally, perspectives for future studies especially in the Indonesian population will be sketched.
WIF1 Qualitative-Methylation from Peripheral Blood Could Not Be Used as Biomarker for The Risk of Nasopharyngeal Carcinoma or Smoking Behavior in Yogyakarta Panel Aditya Kurniawan; Erika Diana Risanti; Saihas Suhda; Hanggoro Tri Rinonce; Ery Kus Dwianingsih; Jajah Fachiroh
The Indonesian Biomedical Journal Vol 11, No 3 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i3.810

Abstract

BACKGROUND: Tobacco smoking plays an important role in nasopharyngeal carcinoma (NPC) risk. The Wingless-related integration site (WNT) Inhibitory Factor 1 (WIF1) as one of the tumor suppressor gene (TSG), functions to control cell proliferation through suppression of WNT signaling pathway. WIF1 methylation is one of epigenetic mechanisms which causes overactivated of WNT pathway in NPC development. WIF1 methylation from buffy coat related to smoking status and NPC risk is not known yet.METHODS: This was a nested case-control study involving 39 newly diagnosed NPC patients and 40 healthy controls. All subjects were enrolled at Dr. Sardjito Hospital, Yogyakarta. Subjects were male, with known smoking status, duration, and quantity of cigarette consumed. Parallel DNA isolated from buffy coat was used for WIF1 DNA methylation analysis, by using methylation-specific PCR (MSP) method. Chi-square analysis was used to determine distribution of differences among groups. Two tailed p-value≤0.05 was considered as statistically significant.RESULTS: No significant difference between WIF1 methylation of cases and controls (p=0.30), nor smoking habit among smokers (p=0.51) and non-smokers (p=0.43).CONCLUSION: WIF1 methylation from buffy coat could not be used as an NPC marker nor as a smoking behavior marker.KEYWORDS: buffy coat, cigarette smoking, methylation, nasopharyngeal carcinoma, WIF1
Development of Tetra-primer Amplification Refractory Mutation System (ARMS) PCR for Detection of CHRNA3 rs8040868 Anggi Laksmita Dewi; Dewi Kartikawati Paramita; Jajah Fachiroh
The Indonesian Biomedical Journal Vol 13, No 2 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i2.1463

Abstract

BACKGROUND: Single nucleotide variations (SNV) have been mapped to be associated with several human conditions and diseases. To validate the association between SNV to certain human traits or diseases, a large number of subjects must be included. Thus, in need of a fast, relatively economic, and reliable genotyping method. This can be achieved through the use of tetra-primer amplification refractory mutation system polymerase chain reaction (Tetra-primer ARMS PCR). This study reports strategy to develop Tetra-primer ARMS PCR-based genotyping of CHRNA3 rs8040868.METHODS: The optimization of Tetra-primer ARMS PCR was done through these steps: identification of gene sequence and position of single mutation; designing outer and inner PCR primers; amplification of target gene fragments through PCR by using outer primer; confirming genotype of the PCR product by using sequencing; determining an optimum ratio of outer and inner primer; and determining optimum annealing temperature and cycles for the PCR program. The PCR products were run in 2% gel agarose electrophoresis and visualized under UV illumination.RESULTS: Outer and inner primer ratio of 1:3 with annealing temperature of 64.4°C and 40x cycles was found to be the most optimum condition. Tetra-primer ARMS PCR was able to confirm the results of the DNA sequence of 2 samples, confirming wild-type variants (TT allele) and the heterozygous mutant (CT allele).CONCLUSION: Tetra-primer ARMS PCR was able to genotype rs8040868 of the CHRNA3 gene.KEYWORDS: tetra-primer ARMS PCR, CHRNA3, rs8040868, genotyping
Association of Peripheral Blood RASSF1A and CDKN2A Methylation Status with Smoking Behaviour in Nasopharyngeal Carcinoma Erika Diana Risanti; Aditya Kurniawan; Laila Wahyuningsih; Ery Kus Dwianingsih; Hanggoro Tri Rinonce; Jajah Fachiroh
The Indonesian Biomedical Journal Vol 10, No 2 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i2.381

Abstract

BACKGROUND: Hypermethylation of RASSF1A and CDKN2A is one of epigenetic factor underlies nasopharyngeal carcinoma (NPC) development. Smoking behavior as an NPC’s risk factor causes aberrant DNA methylation. RASSF1A and CDKN2A promoter hypermethylation from peripheral blood cells correlates with smoking behavior. The use of body fluids including peripheral blood as a specimen for DNA methylation analyzes are widely developed, as less invasive method compared to the use of tissue biopsy. This study aims to observe the association between RASSF1A and CDKN2A methylation in peripheral blood and smoking behavioramong NPC patients.METHODS: Newly diagnosed NPC subjects were recruited from ear-nose-throat (ENT) outpatient clinic of Dr. Sardjito Hospital, Yogyakarta. DNA from buffycoat of 19 smokers and 20 non-smokers NPC’s patients were isolated. Bisulphite modification was applied to 500 ng of the isolated DNA. The methylation status was detected by MSP (methylation-specific polymerase chain reaction (PCR)). The association between smoking status and promoter hypermethylation was analysis using Chi-Square test.RESULTS: MSP analysis of RASSF1A showed that 68.42% smoker and 75% non-smoker NPC’s patients were methylated. MSP analysis of CDKN2A showed that 21.05% smoker and 25% non-smoker NPC’s patients were methylated. There was no association between smoking behavior with RASSF1A and CDKN2A methylation (p>0.05).CONCLUSION: Statistical analysis showed that smoking behavior is not associated with methylation of RASSF1A and CDKN2A among NPC’s patients.KEYWORDS: DNA methylation, CDKN2A, RASSF1A, Nasopharyngeal carcinoma, Smoking
Biobank ensuring sustainability in health research Jajah Fachiroh;     
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 1, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss1.art2

Abstract

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Co-Authors Aditya Kurniawan Aditya Kurniawan Anggi Laksmita Dewi Anggi Laksmita Dewi Dewajani Purnomosari Dewi Kartikawati Paramita Dewi Kartikawati Paramita Dewi Kartikawati Paramita Dian Eurike Septyaningtrias Dwianingsih, Ery Kus Erika Diana Risanti Hanggoro Tri Rinonce Hanggoro Tri Rinonce Laila Wahyuningsih Nastiti Wijayanti Rina Susilowati Saihas Suhda To'bungan, Nelsiani      ‘Aliyah, Siti Hamidatul