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UJI STABILITAS POOLED SERA YANG DISIMPAN DALAM FREEZER UNTUK PEMANTAPAN MUTU INTERNAL DI LABORATORIUM KLINIK Anik Handayati; Juliana Christyaningsih; Tjipto Rini
JURNAL PENELITIAN KESEHATAN Vol 12, No 1 (2014): Jurnal Penelitian Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.559 KB)

Abstract

Stability of control sera is very important in order to assess the performance of a laboratory, including quality instruments and reagents. Some small laboratories are using pooled sera as a control sera for day-to- day examination. In general, the temperature of the freezer is used to save serum in - 7 to - 4the freezer is frequently opened  and closed so it is likely the temperature is too unstable. This study was an experiment laboratory, with  the time series control group design. The serum was analyzed the influence of storage time in the freezer at a temperature of -7 to -4ºC and a temperature of – 15stability of the normal and abnormal of pooled sera and examination were using the parameters of glucose, cholesterol and uric acid in serum. Samples were divided into 9 groups: 0,  1, 2 , 3 , 4 , 5, 6, 7 , 8 weeks. Data were analyzed with regression equations. The storage effect on normal and abnormal of blood glucose level in the freezer temperature -7 º to -4 º C for 8 weeks but at - 15temperature had no effect on both normal and abnormal of serum glucose level. There was no effect of storage in a freezer temperature -7 º to -4 º C and -15 º C for 8 weeks on cholesterol levels and normal and abnormal uric acid. Key Word: stability, control of sera, freezer, internal quality, clinical laboratory
The Effectiveness of Moringa Oleifera Leaf Extract on Hepatotoxic Case Reviewing from Cadmium, SGOT and SGPT Blood Levels in white Rats (Rattus norvegicus) Induced with Cadmium (Cd) Devi Puspitasari; Indah Lestari; Christ Kartika Rahayuningsih; Juliana Christyaningsih
Jurnal Teknokes Vol 15 No 3 (2022): September
Publisher : Jurusan Teknik Elektromedik, POLTEKKES KEMENKES Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/teknokes.v15i3.278

Abstract

Moringa leaf (Moringa oleifera Lamk.) is part of herbal plants that have been known for their good natural chelating agent and hepatoprotective properties, especially in hepatotoxic events due to cadmium (Cd) exposure, where flavonoid compounds are contained in the form of quercetin and kaempferol, vitamin A, vitamin C and several phenol components that can inhibit the accumulation of cadmium metals and neutralize free radicals by lowering SGOT and SGPT levels, so this study aimed to determine the effectiveness of moringa leaf extract (Moringa oleifera Lamk.) against hepatotoxic in white rats (Rattus norvegicus) induced with cadmium. This study was expected to provide potential of Moringa leaves in hepatotoxic case at dose 400 mg/kgwb, 500 mg/kgwb and 600 mg/kgwb in white rats. This research was an experimental study with quantitative analysis conducted at the Faculty of Veterinary Medicine, Airlangga University, Surabaya Health Laboratory Center, and Bakti Analisa Laboratory in November 2021 – July 2022. The independent variable was the dose of Moringa leaf extract and the dependent variables were levels of cadmium, SGOT and SGPT in white rat blood. Analysis of blood cadmium levels using an atomic absorption spectrophotometer and analysis of SGOT SGPT levels using the BS-200 Chemistry Analyzer. The results showed that the average blood cadmium, SGOT and SGPT levels in treatment group 1 were 0.0605 μg/dL, 260.175 U/L, and 143.85 U/L; in treatment group 2 were 0.075 μg/dL, 250.925 U/L and 77.575 U/L; in treatment group 3 were 0.08125 μg/dL, 171.475 U/L and 66.075 U/L. Based on statistical analysis of One-way Anova test, sig. p value > 0.05, there was no significant difference in cadmium and SGOT levels. Then, the results of the Kruskal wallis test on SGPT levels showed sig. p value > 0.05, there was no significant difference in all treatments. Moringa leaf extract at a dose of 600 mg/kgbw/day had the best effect as chelating agent and hepatoprotective compared to doses of 400 mg/kgbw/day and 500 mg/kgbw/day at cadmium induction for 10 days. From this study, we recommend to consume about 66,522 g/day Moringa fresh leaves as the natural chelating agent and hepatoprotector for overcoming acute hepatotoxic case. INDEX TERMS Moringa Leaves, Cadmium, SGOT, SGPT, Rattus norvegicus, Atomic Absorption Spectrophotometer, Chemistry Analyzer
PEMERIKSAAN DENYUT NADI, TEKANAN DARAH, DAN GLUKOSA DARAH DI POSYANDU LANSIA ABIYOSO POLKESBAYA Ervi Husni; Tatarini Ika Pipit Cahyani; Sherly Jeniawaty; Dina Isfentiani; Ani Media Harumi; Ira Puspitasari; Ira Rahayu Tiyar Sari; Yuni Ginarsih; Dwi Purwanti; Ulfa Hidayah; Puspita Zella Wigati; Luthfi Rusyadi; Juliana Christyaningsih; Melania Rizerda Pebianti
Edukasi Masyarakat Sehat Sejahtera (EMaSS) : Jurnal Pengabdian kepada Masyarakat Vol. 6 No. 2 (2024): Juli
Publisher : Poltekkes Kemenkes Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37160/emass.v6i2.400

Abstract

Upaya pemeliharaan kesehatan lansia dilakukan sejak lansia berusia 60 tahun dengan tujuan untuk menjaga agar tetap hidup sehat, berkualitas, dan mandiri. Kesehatan fisik dan mental menjadi faktor penting yang perlu diperhatikan pada lansia. Kegiatan pengabdian kepada masyarakat yang diselenggarakan pada tanggal 6 September 2023, berlokasi di Poltekkes Kemenkes Surabaya, dihadiri 38 lansia. Alat yang digunakan untuk pemeriksaan tekanan darah dan denyut nadi menggunakan alat tensimeter sedangkan pemeriksaan glukosa darah acak menggunakan alat point of care. testing (POCT). Hasil yang didapat adalah lansia yang ikut program ini terbanyak 68% berjenis kelamin perempuan, selain itu ada 12 dari 26 (32%) lansia memiliki memiliki tekanan darah tinggi. Terdapat 1 dari 38 (3%) lansia memiliki denyut nadi di atas batas normal dan 3 dari 38 (8%) lansia dengan kadar glukosa darah di atas batas normal. Hal ini menunjukkan bahwa kondisi kesehatan lansia harus tetap dipantau anggota keluarga lainnya agar lansia selalu sehat, Kata kunci: Denyut nadi, tekanan darah lansia, kadar glukosa darah, Posyandu lansia Abiyoso Polkesbaya