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All Journal Jurnal Penelitian Kesehatan THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Kesehatan Prima Jurnal Ilmu dan Teknologi Kesehatan JURNAL PENELITIAN KESEHATAN Jurnal Analis Medika Biosains (JAMBS) JPP Jurnal Kesehatan Poltekkes Palembang Jurnal Ilmiah Tatengkorang Jurnal TEKNOKES

Christ Kartika Rahayuningsih

Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Surabaya

Author-ID : 2390630

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Penyimpanan dan Penambahan Kurma Ajwa Terhadap Aktivitas Antioksidan Infused Water Stroberi dan Wortel Luh Putu Rahayu Candra Dewi; Indah Lestari; Christ Kartika Rahayuningsih; Maruni Wiwin Diarti
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.274

Radikal bebas merupakan senyawa yang sangat reaktif dan dapat merusak molekul pembentuk sel dalam tubuh sehingga terjadi kemunduran fungsi organ dan memicu timbulnya penyakit degeneratif. Infused water merupakan alternatif dalam proses detoksifikasi radikal bebas sekaligus pemenuhan kebutuhan cairan tubuh harian. Stroberi banyak dijadikan sebagai bahan utama infused water karena kandungan vitamin C yang tinggi dan dikombinasikan dengan wortel yang kaya akan beta karoten sedangkan kurma ajwa dijadikan sebagai penambah cita rasa. Kandungan antioksidan total dalam infused water mudah mengalami degradasi fisik maupun kimia selama proses penyimpanan. Penelitian ini bertujuan untuk mengetahui pengaruh penyimpanan dan penambahan kurma ajwa terhadap aktivitas antioksidan infused water stroberi dan wortel. Jenis penelitian yang digunakan adalah eksperimental dengan rancangan post test only control group design yang dilaksanakan di Laboratorium Proteomik Institute Tropical Disease Universitas Airlangga dan Laboratorium Kimia AMAMI Jurusan Analis Kesehatan Poltekkes Kemenkes Surabaya pada bulan Oktober 2019 sampai Juni 2020. Penelitian ini terdiri dari dua kelompok infused water dengan dan tanpa penambahan kurma ajwa yang disimpan selama 0 jam sebagai kontrol, 2 jam dengan pengisian ulang pertama dan 4 jam dengan pengisian ulang kedua pada suhu ruang (±28oC) dan suhu refrigerator (±10oC). Masing - masing sampel diuji aktivitas antioksidan menggunakan metode DPPH yang dinyatakan dengan IC50 sedangkan untuk uji organoleptik dilakukan secara hedonik oleh panelis. Hasil analisis ANOVA menunjukkan bahwa penyimpanan dan penambahan kurma ajwa berpengaruh nyata terhadap aktivitas antioksidan infused water stroberi dan wortel (p<0,05). Aktivitas antioksidan tertinggi diperoleh dari infused water dengan penambahan kurma pada penyimpanan suhu ruang selama 2 jam dengan nilai IC50 sebesar 206,53 ppm yang menandakan aktivitas antioksidan sedang.

Indeks Massa Tubuh (IMT), Glukosa Darah Puasa dan HbA1C Pekerja Kantor dengan Obesitas Sentral Anik Handayati; Christ Kartika Rahayuningsih; Winarni Winarni
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 12, No 3 (2021): Juli 2021
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf12319

Central obesity is excess body weight as a result of the accumulation of intra-abdominal and subcutaneous fat that occurs in the abdominal area which causes insulin resistance. Central obesity is caused by excessive food intake and decreased physical activity that often occurs in office workers. Central obesity can cause diabetes mellitus. So the purpose of this study was to analyze the correlation between BMI with fasting blood glucose and HbA1c in office workers with central obesity. The design of this study was cross-sectional. The study population was office workers with central obesity at the Surabaya Prodia Laboratory who met the inclusion criteria. The sample was selected by purposive sampling technique. Data were collected through direct measurement, then analyzed by Spearman's rho test. The results showed that BMI was not correlated with fasting blood glucose (p-value = 0.364), nor was it correlated with HbA1c (p-value = 0.815). Keywords: central obesity; body mass index; fasting blood glucose; HbA1c ABSTRAK Obesitas sentral adalah kelebihan berat badan sebagai akibat dari akumulasi lemak intra abdominal dan subkutan yang terjadi di daerah abdomen yang menyebabkan terjadinya resistensi insulin. Obesitas sentral diakibatkan karena asupan makanan berlebih dan penurunan aktifitas fisik yang sering terjadi pada pekerja kantor. Obesitas sentral dapat menyebabkan penyakit diabetes mellitus. Sehingga tujuan penelitian ini adalah menganalisis korelasi antara IMT dengan glukosa darah puasa dan HbA1c pada pekerja kantor dengan obesitas sentral. Rancangan penelitian ini adalah cross-sectional. Populasi penelitian adalah pekerja kantor dengan obesitas sentral di Laboratorium Prodia Surabaya yang memenuhi kriteria inklusi. Sampel dipilih dengan teknik purposive sampling. Data dikumpulkan melalui pengukuran langsung, lalu dianalisis dengan uji Spearman’s rho. Hasil penelitian menunjukkan bahwa IMT tidak berkorelasi dengan glukosa darah puasa (p-value = 0,364), juga tidak berkorelasi dengan HbA1c (p-value = 0,815). Kata kunci: obesitas sentral; indeks massa tubuh; glukosa darah puasa; HbA1c

Potensi Metabolit Sekunder Isolat Aktinomycetes Sebagai Penghasil Senyawa Antibakteri Terhadap Methicillin Resistant Staphylococcus aureus (MRSA) Dari Tanah Mangrove Wonorejo Surabaya Anita Dwi Anggraini; Ayu Puspitasari; Christ Kartika Rahayuningsih
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.7672

Infectious diseases are estimated to be the cause of half of all deaths worldwide. About 50-75% of deaths in hospital are reported to be due to infection. This number continues to increase rapidly in line with the development of bacterial resistance to certain antibiotics. One of the causes of resistance due to antibiotic abuse. The number of resistance events that occur encourages new discoveries in order to overcome the problem of resistance. Actinomycetes are Gram-positive microscopic bacteria that are widespread in nature. These bacteria can live in various environmental conditions that contain lots of nutrients. Actinomycetes population increases in the presence of organic matter that undergoes decomposition. Acinomycetes have the ability to produce antimicrobial compounds. The need for antibiotic compounds and chemotherapy compounds that are effective with low toxicity to the host, and the resulting waste can be degraded by the environment, is a problem that needs serious attention. The existence of resistance to antibiotics causes the need to develop new types of antibiotics from natural ingredients and microorganisms that can control microbapatogens. Compounds obtained from natural materials, especially plants and microbes, provide promising results in the development of new antibiotic compounds, among the existing types of microorganisms, Actinomycetes are the most potential source of producing antibiotics. Apart from antibiotics, actinomycetes also produce bioactive compounds of high economic value in the health sector as antiviral and anti-cancer, while in agriculture as herbicides, insecticides, and anti-parasitic compounds. This causes the need to explore actinomycetes which have the potential to produce antibiotic compounds against bacteria. This study aims to determine the antibacterial activity of secondary metabolites of actinomycetes isolates against cuts infected with Staphylococcus aureus resistant methicillin (MRSA) in winstar rats (rattus norvegicus). This type of research is carried out in an experimental laboratory. The results showed that the potential for secondary metabolites of actinomysetes isolates against MRSA bacteria was classified as strong with an inhibitory index of 10.1.

PENGARUH PEMBERIAN AIR KELAPA MUDA UNTUK MENINGKATKAN KADAR KALIUM DARAH PADA MENCIT Christ Kartika Rahayuningsih
Jurnal Ilmu dan Teknologi Kesehatan Vol 3 No 2 (2016): Maret
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.478 KB)

Young coconut water (Cocos nucifera Linn) is a natural isotonic beverage which contains most of the potassium that can replace lost minerals in the body and has great potential to prevent dieses, so this study aims to determine the effect of coconut water (Cocos nucifera lin) to increase levels potassium blood in mice (Mus musculus). This study is experimental with design 'Postes Only Control Group Design' usingmultisensor to 24 mice of the same species is Mus musculus into 4 groups (6 mice pergroup) with the criteria of sex male mice, aged 2-3 months, weight about 20-40 g withnormal potassium levels from 4.30 to 5.60 mmol / L. Of the samples analyzed obtainedblood potassium levels in mice that is group A without drinking coconut water (control)of 4.65%, group B before taking 1 cc of coconut water amounted to 4.62% and afterdrinking increases to 7.53%, group C before drinking 0.5 cc coconut water amounted to4.87% and after taking into 6.27%, group D before drinking coconut water 0.1 cc of4.20% and after taking into 5.40%. Results of One Way Anova analysis we canconclude that there is the effect of coconut water (Cocos nucifera lin) to increase bloodpotassium levels in mice (Mus musculus).

PENGARUH PENAMBAHAN MINYAK ZAITUN SAAT PEREBUSAN TERHADAP KADAR LIKOPEN PADA WORTEL Christ Kartika Rahayuningsih
Jurnal Ilmu dan Teknologi Kesehatan Vol 3 No 2 (2016): Maret
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.87 KB)

Lycopene is an antioxidant carotenoid class of compounds found in many vegetables and fruits that are red and orange as carrots. Lycopene levels may be increased in the processing of the oil medium, but will decrease in the heating process such as boiling. Purpose of the experiment was to determine the effect of olive oil in the boiling process in maintaining the levels of lycopene in carrots. This study is experimental with quantitative analysis using spectrophotometric method with extraction at 10 carrot samples drawn at randomly in the districts Semampir Surabaya criteria carrots same size and color. Of the samples analyzed earned on average levels of lycopene in carrots boiled for + 10 minutes at 100 °C without the addition of olive oil amounted to 0.2633% and the average levels of lycopene in carrots boiled for + 10 minutes at 100 °C with the addition of 1 ml of oil Olive at 0.3236%, the addition of 2 ml of olive oil amounting to 0.4077%, the addition of 3 ml of olive oil amounting to 0.4904%. The results of One Way Anova analysis showed that the levels of lycopene in carrots increased with the addition of olive oil and the more olive oil is added when boiling, the higher the levels of lycopene in carrots.

THE SUCROSE LEVEL’S DIFFERENCES OF PACKAGED DATES AND BULK PURCHASING DATES THROUGH IODOMETRI LUFF SCHROOL METHODS Indah Lestari; Christ Kartika Rahayuningsih; Aprilia Irmawati
Jurnal Kesehatan Prima Vol 10, No 1 (2016): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Fruits, vegetables and honey contain a high sucrose, when digested (hydrolyze), sucrose will be seperated into glucose and fructose. Chemically, sugar level on dates consists of sucrose, glucose and fructose. Basically, food packaged aims to maintain the quality of freshness, giving simplicity of storage and distribution. Above all, it can reduce the air contamination, water, and soil. Therefore, the purpose in this research was to find out the differences of sucrose level on packaged dates and bulk purchasing dates. This study was a comparative quantitative analysis technique using iodometry method with Luff Schrool to each 4 sample of packaged dates and bulk purchasing dates which were chosen selectively at the Ampel Surabaya market with sample criteria, packaged dates with no brand and bulk purchasing dates in plastic. From the samples analyzed, sucrose concentration obtained was average 9.73% of packaged dates and bulk purchasing dates was14.45%. The analysis results with normality test and T test two indipendent samples can be concluded that there were differences between the sucrose level of packaged dates and bulk purchasing dates. People should consume bulk purchasing dates due to it can be used as an alternative solution to obtain higher substitute energy than packaged dates, it is caused by sucrose level on bulk purchasing dates is higher.

THE EFFECT OF ADDING CYPERUS ROTUNDUS TO DECREASE PEROXIDE LEVEL AT WASTE COOKING OIL Christ Kartika Rahayuningsih; Indah Lestari; Inggit Rianingtyas
Jurnal Kesehatan Prima Vol 10, No 1 (2016): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Jelantah (waste cooking oil) is the oil which is used repeatedly and posseses adequate high peroxide level, which can lead to cancer when the oil is used continually. The addition of antioxidants in the oil is aimed to inhibit the oxidation process, where the Cyperus rotundus is one of the wild plants that contains a high potential as natural antioxidants. Therefore, the purpose in this research is to find out the influence of additional Cyperus rotundus to decrease peroxide level at waste cooking oil. The method applied in this research was experimental with quantitative analysis using iodometric method to 6 samples of waste cooking oil which were randomly choosen. A sample criterion was waste cooking oil which had been used for more than three times by fried seller at Beluru village, Sidoarjo district. Based on the samples analyzed, average peroxide value of waste cooking oil before the additional was a large amount of 16.5309 mEq / 1000 grams, the average value of peroxide after the addition of Cyperus rotundus as much as 5 g of 10.9604 mEq / 1000 g, and the addition 30 g Cyperus rotundus a large amount of 7.3508 mEq / 1000 g, the addition of 50 g Cyperus rotundus a large amount of 5.0103 mEq / 1000 g. The research was analyzed by One Way Anova, and it can be concluded that there was no significant effect on the peroxide of wastecooking oil with the addition of Cyperus rotundus.

THE ADDITION OF SIWALAN BORASSUS FLABELLIFER WATER AGAINTS TOTAL HARDNESS LEVEL IN WELL WATER Christ Kartika Rahayuningsih; Indah Lestari
Jurnal Kesehatan Prima Vol 11, No 2 (2017): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Hardness is the ability of water to form foam when it is mixed with soap. If calcareous water enters the body in excessive amounts in long periods of time can lead to kidney stones disease thus the hardness level in the water must be decreased and one of the methods is the implementation of Siwalan Borassus Flabellifer water, containing lactic acid and alcohol which is capable of dissolving minerals Calcium (Ca2+) in water. The purpose of this study was to determine the effect of the addition of Siwalan Borassus Flabellifer water againts total hardness levels in well water. The analysis is conducted quantitatively and it was experimental study with complexometric titration method to 5 well water samples taken in the Tuban area by grab sampling technique. From the analyzed samples obtained the average value of total hardness control (without the addition of Siwalan Borassus Flabellifer water) 2626.26 mg/L CaCO3, with the addition of 10 mL Siwalan Borassus Flabellifer water of 2346.54 mg/L CaCO3, the addition of 20 mL of Siwalan Borassus Flabellifer water of 2284.38 mg/L CaCO3, and the addition of 30 mL of Siwalan Borassus Flabellifer water of 2206.68 mg/L CaCO3. The analysis results with statistical test One Way Anova can be concluded that there was no significant effect on the total hardness level of well water with the addition of Siwalan Borassus Flabellifer water.

FATTY LEVEL ON FRIED CHICKEN FLOUR SOLD BY FAST FOOD RESTAURANT AND STREET FOODS Christ Kartika Rahayuningsih; Indah Lestari
Jurnal Kesehatan Prima Vol 12, No 1 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Fat as a source of the greatest energy than carbohydrates and protein, and also serves as a source of vitamin A, D, E, and K. Fat consumption in excessive amounts can cause a coronary heart disease, high blood pressure, cholesterol, and atherosclerosis. Fatty level in fast food is high, such as the fried chicken and the current public interest on the fried chicken is extremely high. The objective study was to know a difference of fatty level on fried chicken at fast food restaurant and street food. This research was comparative with analysis technique against 10 fried chickens at fast food restaurants and also 10 friend chickens from street food which were taken selectively around the area of East Surabaya by using Soxhlet method that conducted at the Chemical Laboratory Unit of Surabaya Health Polytechnic. The analysis result was obtained the average value of the fatty level on fried chicken at fast food restaurant was 19.71% and street food of 25.29%. It can be concluded that there are differences in the levels of fat on fried chicken flour at fast food restaurants and street foods.Keywords: Levels of Fat; Fried Chicken Flour.

PROSES PENGOLAHAN IKAN BANDENG (Chanos chanos) TERHADAP KADAR PROTEIN Christ Kartika Rahayuningsih; Sri Sulami Endah Astuti
JURNAL PENELITIAN KESEHATAN Vol 15, No 1 (2017): Jurnal Penelitian Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.264 KB)

Ikan merupakan salah satu bahan pangan penghasil protein terbesar yang sangat bermanfaat bagi tubuh manusia maupun hewan, salah satu contoh adalah ikan bandeng. Beberapa industri rumah tangga mengolah ikan bandeng dengan berbagai macam olahan. Penelitian ini dilakukan dengan tujuan untuk menganalisis kandungan protein pada ikan bandeng dengan beberapa proses pengolahan, yaitu pengasapan, pemindangan, dan penggorengan, serta bertujuan untuk mengetahui ada tidaknya pengaruh beberapa cara pengolahan ikan bandeng terhadap kadar protein. Jenis penelitian eksperimental dengan analisa kuantitatif menggunakan metode Kjeldahl yang dilakukan di Balai Riset dan Standarisasi Industri (Baristan) Surabaya. Sampel penelitian adalah ikan bandeng segar sebagai kontrol, ikan bandeng yang diasap, ikan bandeng yang dipresto dan ikan bandeng yang digoreng. Berdasarkan hasil penelitian, diperoleh kadar protein ikan bandeng segar sebagai kontrol sebesar 19,79%, ikan bandeng asap sebesar 23,33%, ikan bandeng presto sebesar 21,59%, dan ikan bandeng goreng sebesar 26,17%. Uji statistik menunjukkan bahwa ada pengaruh cara pengolahan ikan bandeng (Chanos chanos) terhadap kadar protein.Kata Kunci : Ikan Bandeng, Kadar Protein, Proses Pengolahan

Co-Authors Alfiah, Siti Anik Handayati Anita Dwi Anggraini Aprilia Irmawati Aprilyadi, Nadi Astuti Setiyani Ayu Puspitasari Bambang Hadi Sugito Darjati Darjati Dedi Kurniawan Demes Nurmayanti Devi Puspitasari Devi Puspitasari Diah Titik Mutiarawati Evi Yunita N Hadi Suryono, Hadi Hotmaida Siagian Imam Khambali Indah Lestari Indah Lestari Indah Lestari Indira Ayuni Sri Pramasti Inggit Rianingtyas Ira Puspita Sari Isfentiani, Dina Juliana Christyaningsih Juliana Christyaningsih Kiaonarni OW Lembunai Tat Alberta, Lembunai Tat Luh Putu Rahayu Candra Dewi Lully Hani Endarini Lully Hanni Endarini Maruni Wiwin Diarti N. S. Widodo Ni Made Risma Fridayanti Nur Hatijah Retno Sasongkowati Rieke Dwi Ramadhany Riya Agustin Riya Agustin Sri Sulami Endah Astuti Suliati Suliati Sumasto, Hery Tacik Idayanti Teresia Retna Puspitadewi Vernanda Arsya Nabilla Winarni Winarni Woelansari, Evy Diah Yusianti Silviani

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