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All Journal Jurnal Penelitian Kesehatan Health Notions JURNAL PENELITIAN KESEHATAN JURNAL MEDIA KESEHATAN INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY AloHA International Journal of Health Advancement (AIJHA) International Journal of Advanced Health Science and Technology Frontiers in Community Service and Empowerment
Anik Handayati
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya
Humanities Biochemistry, Genetics & Molecular Biology Dentistry Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Indeks Massa Tubuh (IMT), Glukosa Darah Puasa dan HbA1C Pekerja Kantor dengan Obesitas Sentral Anik Handayati; Christ Kartika Rahayuningsih; Winarni Winarni
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 12, No 3 (2021): Juli 2021
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf12319

Abstract

Central obesity is excess body weight as a result of the accumulation of intra-abdominal and subcutaneous fat that occurs in the abdominal area which causes insulin resistance. Central obesity is caused by excessive food intake and decreased physical activity that often occurs in office workers. Central obesity can cause diabetes mellitus. So the purpose of this study was to analyze the correlation between BMI with fasting blood glucose and HbA1c in office workers with central obesity. The design of this study was cross-sectional. The study population was office workers with central obesity at the Surabaya Prodia Laboratory who met the inclusion criteria. The sample was selected by purposive sampling technique. Data were collected through direct measurement, then analyzed by Spearman's rho test. The results showed that BMI was not correlated with fasting blood glucose (p-value = 0.364), nor was it correlated with HbA1c (p-value = 0.815). Keywords: central obesity; body mass index; fasting blood glucose; HbA1c ABSTRAK Obesitas sentral adalah kelebihan berat badan sebagai akibat dari akumulasi lemak intra abdominal dan subkutan yang terjadi di daerah abdomen yang menyebabkan terjadinya resistensi insulin. Obesitas sentral diakibatkan karena asupan makanan berlebih dan penurunan aktifitas fisik yang sering terjadi pada pekerja kantor. Obesitas sentral dapat menyebabkan penyakit diabetes mellitus. Sehingga tujuan penelitian ini adalah menganalisis korelasi antara IMT dengan glukosa darah puasa dan HbA1c pada pekerja kantor dengan obesitas sentral. Rancangan penelitian ini adalah cross-sectional. Populasi penelitian adalah pekerja kantor dengan obesitas sentral di Laboratorium Prodia Surabaya yang memenuhi kriteria inklusi. Sampel dipilih dengan teknik purposive sampling. Data dikumpulkan melalui pengukuran langsung, lalu dianalisis dengan uji Spearman’s rho. Hasil penelitian menunjukkan bahwa IMT tidak berkorelasi dengan glukosa darah puasa (p-value = 0,364), juga tidak berkorelasi dengan HbA1c (p-value = 0,815). Kata kunci: obesitas sentral; indeks massa tubuh; glukosa darah puasa; HbA1c
Correlation Between Implementation Intensity of Internal Quality Control and the Outcome of External Quality Control at Public Health Center Laboratories Anik Handayati; Ni Wayan Lisna Marheni
Health Notions Vol 5, No 3 (2021): March
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn50302

Abstract

Quality control is a process in a procedure conducted to evaluate testing process, with the purpose of ensuring the outcome of laboratory examinations, minimizing, as well as identifying the source of deviations. It consists of two, the internal and external quality control. This study aims to determine correlation between implementation intensity of internal quality control and the outcome of external quality control in the parameter of complete blood count in Public Health Centers Laboratories in Gianyar Bali. This study was a correlational observational study with cross-sectional approach. Total samples of 13 laboratories were taken from total population. As many as 15.38% of Public Health Center carried out internal quality control every day, 53.85% once a week, 7.7% biweekly, and 23.07% carried it out once a month. As for external quality control with parameter of complete blood count, 61.54% of the centers were good, 23.07% were average, and 15.38% were poor in handling abnormally low level of complete blood count. At normal level, 76.92% were good, 15.38% in average, and 7.7% were below average. At abnormally high level, 53.85% were good and 46.15% were in average. Statistical test results showed p-value >0.05. It can be concluded that there is no correlation between implementation intensity of internal quality control and the outcome of external quality control with parameter of complete blood count in Public Health Center laboratories in Gianyar Bali. Keywords: internal quality control; external quality control; complete blood count
Correlation of Neutrophil and Lymphocyte Counts in Patient Infected with Coronavirus (Covid-19) Nur Alina Hidayatur Rizki; Anik Handayati; Ellies Tunjung Sari Maulidiyanti
Health Notions Vol 6, No 3 (2022): March
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn60305

Abstract

Coronavirus Disease 2019 (Covid-19) causes hyper-inflammation and poor clinical conditions leading to high mortality in the world. Neutrophil and lymphocyte counts are used to describe disease severity in many clinical conditions including COVID-19. The purpose of this study was to determine the relationship between neutrophils and lymphocyte counts in patients infected with the coronavirus (Covid-19). The type of research used was cross-sectional. The sample in this study were patients infected with the Coronavirus (Covid-19) in a special isolation room who did a complete blood check at Brawijaya Hospital Surabaya. Types of data obtained from LIS (Laboratory Information System). The results of the study on the number of neutrophils in coronavirus (Covid-19) patients at Brawijaya Hospital showed an average neutrophil count of 5,174 cells/uL and an average lymphocyte count of 1,514 cells/uL. Data analysis showed that there was no correlation between the number of neutrophils and lymphocytes in patients infected with the Coronavirus (Covid-19) at Brawijaya Hospital Surabaya, possibly related to differences in immunity in each person. Keywords: coronavirus (Covid-19); neutrophils; lymphocytes
Anticoagulant Activity of Dayak Onion Bulb (Eleutherine bulbosa) Extract on Human Blood Samples Anik Handayati; Syamsul Arifin; Monica Putji Astuti
Health Notions Vol 5, No 2 (2021): February
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn50202

Abstract

Cardiovascular disease can occur due to disruption of the hemostatic system by forming blood clots in blood vessels, or commonly known as deep vein thrombosis. The curative action that has been carried out in the form of heparin anticoagulant administration had many side effects such as bleeding, hematuria, thrombocytopenia, and hypersensitivity; therefore, alternative anticoagulant ingredients such as onion bulbs have emerged. Dayak onion bulb (Eleutherine bulbosa) is known to contain eleutherinol compound, which has anticoagulant activity. This study aims to determine the potential anticoagulant activity of Dayak onion bulbs extract (Eleutherine bulbosa) on human blood samples. This anticoagulant activity test was carried out by observing the length of time for blood clotting in the extract treatment group with several concentrations (0, 10, 12.5, 16, 25, and 50%) by Clotting Time (Lee-White) method. The results showed a significant delay in blood coagulation time in the group treated with Dayak onion bulbs extract as increasing the concentration. About 50% Dayak onion onion bulb extract was showed a twofold increase in blood coagulation time thanthat of no extract administration. So that, Dayak bulb onion has the opportunity to be an alternative natural anticoagulant. Keywords: anticoagulants; Dayak onion bulbs (Eleutherine bulbosa); clotting time
Correlation of Fasting Blood Glucose Levels and HbA1c with Body Mass Index in Type 2 Diabetes Mellitus Patients Anik Handayati; Lully Hanni Endarini; Yuni Eka Cahyana
Health Notions Vol 6, No 4 (2022): April
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn60404

Abstract

Excess body mass index is at greater risk of developing diabetes mellitus than other diseases. Monitoring blood glucose includes through examination of fasting blood glucose levels and HbA1c. This study aims to find out the relationship of fasting blood glucose levels and HbA1c with Body Mass Index in type 2 diabetes mellitus patients. This study was an observational analytical study with a cross sectional design. The research was conducted at Prima Surabaya Main Clinic in January to June 2021. The sample was a type 2 diabetes mellitus patient who conducted fasting blood glucose examination, HbA1c and Body Mass Index measurement at Prima Surabaya Main Clinic as many as 40 people, were taken purposive sampling. The results of this study found that the average fasting blood glucose level of patients with type 2 diabetes mellitus was 172.6 mg / dL and HbA1c was 7.76%. The average Body Mass Index was 24.97. Pearson correlation test results obtained significant Body Mass Index with fasting blood glucose levels p = 0.150 and HbA1c with Body Mass Index p = 0.295, which means there is no relationship between fasting blood glucose levels and HbA1c with body mass index in type 2 diabetes mellitus patients.  Keywords: fasting blood glucose; HbA1c; Body Mass Index; auto analyzer
Representation of NLR (Neutrophil Lymphocyte Ratio) Value in Corona Virus (Covid-19) Patients by Age Group at Gatoel Mojokerto Hospital Juliawan Apurwatama; Anik Handayati; Rinza Rahmawati
Aloha International Journal of Health Advancement (AIJHA) Vol 5, No 1 (2022): January
Publisher : Alliance oh Health Activists (AloHA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/aijha50104

Abstract

Currently the world is faced with the condition of the Corona virus 2019 (Covid-19) disease pandemic whose cases are still increasing over time and widespread around the world. The Covid-19 pandemic is still a health problem throughout the country, not least in Indonesia. Covid-19 is an acute respiratory disease caused by a new type of coronavirus infection or later named Severe acute respiratory syndrome 2 (SARS-CoV-2). The purpose of this study was to find out the difference in the results of neutrophil lymphocyte ratio (NLR) between Covid-19 patients with productive age and and elderly age. In Covid-19 patients with elderly age had a high NLR value above ≥3.13 with a percentage of 83% while in Covid-19 patients of productive age also had a high NLR value above ≥3.13 by 75% but the value was still lower than in Covid-19 patients with elderly age. It could be concluded that Covid-19 patients with elderly age and productive age had differences in NLR values although the same had a high NLR value above ≥3.13 and based on the results of this study in Covid-19 patients with elderly age and productive age had low lymphocyte values or experience lymphopia. Keywords: Covid-19; elderly age; productive age; neutrophil lymphocyte ratio
PENGARUH PENYIMPANAN URINE TERHADAP JUMLAH lEUKOSIT DAN ERITROSIT PADA PENDERITA INFEKSI SALURAN KEMIH DENGAN METODE SY (STANDARD YIELD) Edy Haryanto; Pestariati .; Anik Handayati; Sri Sulami Endah Astuti
JURNAL PENELITIAN KESEHATAN Vol 13, No 1 (2015): Jurnal Penelitian Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1482.879 KB)

Abstract

Urinary tract infection ( un ) is a pathological condition that is often encountered and marked with urinesediment results indicate nitrite, leucocytes and positive bacteria. In some hospitals, inpatient specimendelivery is often delayed, causing delayed examination. Urine samples should be examined, therefore itis kept at room temperature, lysis of erythrocytes and leukocytes and urine will turn into alkaline. Thisstudy aims to prove how much the influence of the long treatment of urinary tract infection to the numberof leukocytes and erythrocytes urine sediment . "This type of research is quantitative analysis " todetermine the number of leukocytes and erythrocytes urine sediment urinary tract infection sufferers whoimmediately performed ( 0 hours) , stored for 2 hours, 3 hours and 4 hours using SY ( standard yield) .The results of the examination number of leukocytes to the 20 sediment samples with immediate unpatients obtained an average of 59 cells / mL , after the delay of 2 hours earned the average of 35 cells /mL, 3 hours on the average of 18 cells / mL and 4 hours obtained an average of 12 cells / uL. The resultsof the examination of erythrocyte sediment amount to ISK 20 patient samples obtained immediatelyperformed the average of 20 cells / mL , after the delay of 2 hours earned the average of 14 cells / mL , 3hours on the average of 9 cells / mL and 4 hours obtained the average of 6 cells / mL . From the Post Hoctest results obtained three treatment groups proved no longer influence to urinary tract infections.suspension 0 hour, 2 hours, and 3 hours to the number of leukocytes and erythrocytes urine sediment.There is no longer influence to urinary tract infections suspension 3 hours and 4 hours on the number ofleukocytes and erythrocytes urine sediment.Key words: ISK, Sedimen urtn, /eukocit dan erithrocit.
UJI STABILITAS POOLED SERA YANG DISIMPAN DALAM FREEZER UNTUK PEMANTAPAN MUTU INTERNAL DI LABORATORIUM KLINIK Anik Handayati; Juliana Christyaningsih; Tjipto Rini
JURNAL PENELITIAN KESEHATAN Vol 12, No 1 (2014): Jurnal Penelitian Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.559 KB)

Abstract

Stability of control sera is very important in order to assess the performance of a laboratory, including quality instruments and reagents. Some small laboratories are using pooled sera as a control sera for day-to- day examination. In general, the temperature of the freezer is used to save serum in - 7 to - 4the freezer is frequently opened  and closed so it is likely the temperature is too unstable. This study was an experiment laboratory, with  the time series control group design. The serum was analyzed the influence of storage time in the freezer at a temperature of -7 to -4ºC and a temperature of – 15stability of the normal and abnormal of pooled sera and examination were using the parameters of glucose, cholesterol and uric acid in serum. Samples were divided into 9 groups: 0,  1, 2 , 3 , 4 , 5, 6, 7 , 8 weeks. Data were analyzed with regression equations. The storage effect on normal and abnormal of blood glucose level in the freezer temperature -7 º to -4 º C for 8 weeks but at - 15temperature had no effect on both normal and abnormal of serum glucose level. There was no effect of storage in a freezer temperature -7 º to -4 º C and -15 º C for 8 weeks on cholesterol levels and normal and abnormal uric acid. Key Word: stability, control of sera, freezer, internal quality, clinical laboratory
Diagnostic test of Rapid antigen SARS Cov-2 against RT-PCR on suspected symptomatic COVID-19 patients at Rato Ebu Hospital Bangkalan Anik Handayati; Edy Haryanto
International Journal of Advanced Health Science and Technology Vol. 2 No. 3 (2022): June
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v2i3.3

Abstract

COVID-19 is caused by the SARS-CoV-2 virus. Two tests to detect the SARS-CoV-2 virus are the rapid antigen test and RT-PCR. The gold standard for testing for COVID-19 is RT-PCR. The high number of Covid-19 sufferers in Madura plus the RT-PCR examination takes a long time. Rapid antigen examination is one of the Covid-19 screening solutions that should be tested because it offers fast examination times. This study aims to determine the sensitivity and specificity of the SARS COV-2 rapid antigen test to RT PCR for the diagnosis of COVID 19. This type of research is an analytical study with a cross-sectional design. The study was conducted at Rato Ebu Hospital Bangkalan from July-September 2021. The sample of this study was suspected symptomatic COVID-19 patients who were examined using the SARS-CoV-2 rapid antigen and RT-PCR using purposive sampling of as many as 60 people. Diagnostic test method by measuring the sensitivity and specificity of rapid antigen to RT-PCR. Based on the results of the study, it can be concluded that the sensitivity of the SARS Cov-2 Rapid Antigen to RT-PCR is 82.97% and the specificity of the SARS Cov-2 Rapid Antigen to RT-PCR is 100%.
Description of Platelet Count Patients Infected with Corona Virus (Covid-19) with Grouping of Productive and Elderly Age Hapipah Hapipah; Anik Handayati; Nur Vita Purwaningsih
International Journal of Advanced Health Science and Technology Vol. 2 No. 1 (2022): February
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.272 KB) | DOI: 10.35882/ijahst.v2i1.2

Abstract

Routine hematology tests are one of the supporting tests in diagnostics to assess the severity of the disease and predict risk in COVID-19 patients, and can be used as supporting data on RT-PCR tests. Some blood tests that are widely used as monitoring tools and predicators of COVID-19 are the number of leukocytes, the  number of lymphocytes, the number of neutrophils, the  number of platelets and NLR (Neutrophil lymphocyte ratio).  Thrombocytopenia occurs due to excessive use of tromocytes in the body as compensation for damage to lung parenchym cells, the lungs are organs where platelets are released from megacaryocytes, so this damage causes disruption of platelet maturation process. The type of research used is quantitative descriptive with the aim to find out the results of blood tests complete with platelet parameters in patients infected with the COVID-19 coronavirus at Muhammad Noer Pamekasan General Hospital. The results of the study were platelet count test results at normal productive age of 67 respondents (96%) and decreased platelet count (Thrombocytopenia) by 3 respondents (4%). While in normal elderly age a number of 26 respondents (87%) and a decreased platelet count (Thrombocytopenia) amounted to 4 respondents (13%).
Co-Authors Anggie Banowati Wibiseno Anita Dwi Anggraini Christ Kartika Rahayuningsih Edy Haryanto Edy Haryanto Ellies Tunjung Sari Maulidiyanti Evy Diah Woelansari Fadia Rakhmalia Fitria Yulfirda Arini Hapipah Hapipah Juliana Christyaningsih Juliawan Apurwatama Kadek Profit Hartani Lully Hanni Endarini Mohd Nazil Salleh Monica Putji Astuti Nada Nabilah Wulandari Ni Made Risma Fridayanti Ni Wayan Lisna Marheni Nur Alina Hidayatur Rizki Nur Vita Purwaningsih, Nur Vita Pestariati Pestariati Pestariati Pestariati Rinza Rahmawati Samsudin Sri Sulami Endah Astuti Sri Sulami Endah Astuti Suhariyadi Suhariyadi Syamsul Arifin Tjipto Rini Winarni Winarni Yuni Eka Cahyana