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All Journal Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention JFIOnline
. Sismindari
Fakultas Farmasi UGM Yogyakarta
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology

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VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KLT DENSITOMETRI Sugihartini, Nining; Fudholi, Achmad; Pramono, Suwidjiyo; Sismindari, .
PHARMACIANA Vol 2, No 1: Mei 2012
Publisher : PHARMACIANA

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Abstract

KLT Densitometri adalah salah satu metode yang banyak digunakan untuk penetapan kadar bahan aktif. Salah satu bahan aktif tersebut adalah epigalokatekin galat dalam ekstrak teh hijau yang banyak digunakan dalam produk krim. Metode tersebut perlu divalidasi untuk membuktikan hasil pengukuran mendekati kadar yang sesungguhnya. Tujuan penelitian ini ingin membuktikan bahwa metode KLT Densitometri yang digunakan akan memberikan linieritas, ketelitian, ketepatan yang memenuhi persyaratan serta mengetahui nilai LOD(Limit if Detection) dan LOQ (Limit of Quantitation). Penelitian menggunakan kadar 600, 1800 dan 3000 ?g dengan replikasi 3 kali untuk mengetahui ketelitian (berdasarkan nilai CV) dan ketepatan (berdasarkan nilai perolehan kembali). Linieritas diketahui dengan menghitung nilai r pada kurva hubungan antara kadar dan luas area kromatogram yang selanjutnya persamaan kurva baku digunakan untuk menghitung LOD dan LOQ. Hasil penelitian menunjukkan bahwa metode yang digunakan memenuhi persyaratan liniearitas dengan nilai r=0,98. Nilai CV untuk kadar 600, 1800 dan 3000 ug masing-masing 8,18%; 3,19% dan 1,53% dan perolehan kembali masing-masing 88,10%; 99,65% dan 111,33%. Nilai LOD adalah 827,01 ?g/ml dan LOQ adalah 2756,69 ?g/ml.
Antiproliferative Effect and Apoptosis Induced in Human Cell Lines by Bruguiera gymnorhiza Barks Methanol Extract Warsinah, .; Sismindari, .; Susidarti, Ratna Asmah
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

The Antiproliferative effects of methanol extract from Bruguiera gymnorhiza (B. gymnorhiza) barks were tested in vitro against three human cell lines: Hela, Raji and Myeloma cells. The extract was found to have antiproliferative effects against Hela, Raji and Myeloma cells with an IC50 value of 133, 504 and 384 µg/mL, respectively. The antiproliferative test was then performed on Hela, Raji,  and  Myeloma  cells.  Cytotoxicity  assay  of  the  extract  was  then  determined  using  MTT method.  There  were  some  indications  of  apoptosis,  such  DNA  fragmentation,  as  assessed  by acrydine orange- ethidium bromide staining. These results indicate that extract from B. gymnorhiza barks can induce apoptosis in human cell lines.Key words: Antiproliferative, Apoptosis, B. gymnorhiza
Antiproliferative Activities of Dianella nemorosa Lam. Leaves Methanol Extract Against HCT-116, C2C12 and 293A Cell lines Karim, Aditya Krishar; Asmara, Widya; Sismindari, .; Istriyati, .; Nohno, Tsutomu
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 2 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Dianella nemorosa Lam. (Liliaceae) is a medicinal plant traditionally used in Papua for treatment of cancer, injury, fracture, inflammation and also for antiseptic. The aim of this study was to evaluate antipriliferative activities of methanol extract of D. nemorosa leaves against HCT+116 (colorectal cell line), C2C12 (adherent mouse myoblast cell line) and 293A (Primary embryonal human kidney transformed with human adenovirus type 5 DNA). Powder of leaves was extracted using methanol. Antiproliverative activities were determined by cell proliferation reagents WST-1 assay for 1 h, 2h and 4h after 72h incubation. The result showed that methanol extract of D.nemorosa leaves showed remarkable antiproliferatiove activities against HTC-116 cell line with IC50 values of 199.31 μg/ml (1h), 197.87 μg/ml (2h) and 161.12 μg/ml (4h). The activities against C2C12 cell line resulted in the IC50 values of 405.51 μg/ml (1h), 435.12 μg/ml (2h) and 394.38 μg/ml (4h), while the IC50 values for 293A cell line were 580.81 μg/ml (1h), 442.21 μg/ml (2h) and 366.74 μg/ml (4h), respectively. Those results indicated that methanol extract of D.nemorosa leaves posses potential antiproliferative activities against HCT-116, C2C12 and 293A cell line. Further study is necessary to investigate the inhibitory mechanism of methanol extract of D. nemorosa leaves on HCT-116, C2C12 and 293A cell lines.Keywords : Dianella nemorosa Lam, cancer cell, HCT-116, C2C12, 293A
Identifikasi Senyawa Antikanker dari Fraksi Kloroform Kulit Batang Bruguiera gymnorhiza dan Aktivitas Sitotoksiknya Warsinah, .; Sismindari, .; Susidarti, Ratna Asmah
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 2 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Bruguiera gmnorhiza is one of mangrove plant that has not been widely studied as potential anticancer compounds. Previous research noted that the methanol extract of the bark of B.gymnorhiza are cytotoxic towards HeLa cells. The purpose of this study was to determine the compounds in the chloroform fraction and cytotoxic activity in HeLa cells. Stem bark of B. gumnorhiza was extracted with methanol, further fractionation by partitioning method, using of the solvent h-hexane and chloroform. Fraction of chloroform was evaporated, the tested cytotoxic activity against HeLa cells by MTT method and apoptotic induction by double staining method. Faction levels are used for 500, 250, 125, 62.5 and 31.25 mg/mL with 24-hour incubation time. Subsequent fractions were identified by GC-MS method. The result showed that the chloroform fraction contains 21 compounds, 6 in the form of fatty acids and terpenoids that have anticancer cytotoxic activity (IC50) of 134 ug/mL with apoptotic mekanism.Keywords: Apoptosis, Bruguiera gymnorhiza, cytotoxic, Fractionation, HeLa cell
Identifikasi Senyawa Antikanker dari Fraksi Kloroform Kulit Batang Bruguiera gymnorhiza dan Aktivitas Sitotoksiknya Warsinah, .; Sismindari, .; Susidarti, Ratna Asmah
Jurnal Farmasi Indonesia Vol 6, No 2 (2012)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (979.176 KB) | DOI: 10.35617/jfi.v6i2.129

Abstract

Bruguiera gmnorhiza is one of mangrove plant that has not been widely studied as potential anticancer compounds. Previous research noted that the methanol extract of the bark of B.gymnorhiza are cytotoxic towards HeLa cells. The purpose of this study was to determine the compounds in the chloroform fraction and cytotoxic activity in HeLa cells. Stem bark of B. gumnorhiza was extracted with methanol, further fractionation by partitioning method, using of the solvent h-hexane and chloroform. Fraction of chloroform was evaporated, the tested cytotoxic activity against HeLa cells by MTT method and apoptotic induction by double staining method. Faction levels are used for 500, 250, 125, 62.5 and 31.25 mg/mL with 24-hour incubation time. Subsequent fractions were identified by GC-MS method. The result showed that the chloroform fraction contains 21 compounds, 6 in the form of fatty acids and terpenoids that have anticancer cytotoxic activity (IC50) of 134 ug/mL with apoptotic mekanism.Keywords: Apoptosis, Bruguiera gymnorhiza, cytotoxic, Fractionation, HeLa cell
Antiproliferative Activities of Dianella nemorosa Lam. Leaves Methanol Extract Against HCT-116, C2C12 and 293A Cell lines Karim, Aditya Krishar; Asmara, Widya; Sismindari, .; Istriyati, .; Nohno, Tsutomu
Jurnal Farmasi Indonesia Vol 6, No 2 (2012)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.903 KB) | DOI: 10.35617/jfi.v6i2.125

Abstract

Dianella nemorosa Lam. (Liliaceae) is a medicinal plant traditionally used in Papua for treatment of cancer, injury, fracture, inflammation and also for antiseptic. The aim of this study was to evaluate antipriliferative activities of methanol extract of D. nemorosa leaves against HCT+116 (colorectal cell line), C2C12 (adherent mouse myoblast cell line) and 293A (Primary embryonal human kidney transformed with human adenovirus type 5 DNA). Powder of leaves was extracted using methanol. Antiproliverative activities were determined by cell proliferation reagents WST-1 assay for 1 h, 2h and 4h after 72h incubation. The result showed that methanol extract of D.nemorosa leaves showed remarkable antiproliferatiove activities against HTC-116 cell line with IC50 values of 199.31 μg/ml (1h), 197.87 μg/ml (2h) and 161.12 μg/ml (4h). The activities against C2C12 cell line resulted in the IC50 values of 405.51 μg/ml (1h), 435.12 μg/ml (2h) and 394.38 μg/ml (4h), while the IC50 values for 293A cell line were 580.81 μg/ml (1h), 442.21 μg/ml (2h) and 366.74 μg/ml (4h), respectively. Those results indicated that methanol extract of D.nemorosa leaves posses potential antiproliferative activities against HCT-116, C2C12 and 293A cell line. Further study is necessary to investigate the inhibitory mechanism of methanol extract of D. nemorosa leaves on HCT-116, C2C12 and 293A cell lines.Keywords : Dianella nemorosa Lam, cancer cell, HCT-116, C2C12, 293A
Co-Authors . Warsinah Achmad Fudholi Aditya Krishar Karim Istriyati, . Istriyati, . Nastiti Wijayanti Nining Sugihartini Peni Indrayudha Ratna Asmah Susidarti Suwidjiyo Pramono Tsutomu Nohno Widya Asmara